O Tratamento com a fluoxetina (mas não com outros farmacos antidepressivo e ansioíiticos) reverte o déficit de memória aversiva causado por estresse agudo de contenção em camundongos

The physiologist H. Selye defined stress as the nonspecific response of the body to any factors that endanger homeostasis (balance of internal environment) of the individual. These factors, agents stressors, are able to activate the Hypothalamic-Pituitary-Adrenal (HPA) axis, thus resulting in the physiological responses to stress by the release of glucocorticoids that leads to psychophysiological changes, including effects on cognitive functions such as learning and memory. When this axis is acutely stimulated occurs a repertoire of behavioral and physiological changes can be adaptive to the individual. Notwithstanding, when the HPA axis is chronically stimulated, changes may favor the development of, such as anxiety disorders. Some drugs used in the clinic for the treatment of anxiety disorders these can exert effects on cognitive function, on the HPA axis and on the anxiety. In this context, the aim of our study was to investigate the effects of administration i.p. acute of diazepam (DZP, 2 mg/kg), buspirone (BUS, 3 mg/kg), mirtazapine (MIR, 10 mg/kg) and fluoxetine (FLU, 10 mg/kg) in male mice submitted to acute restraint stress, and evaluated using plus-maze discriminative avoidance task (PMDAT), which simultaneously evaluates parameters such as learning, memory and anxiety. Our results demonstrated that (1) the administration of DZP and BUS, but not FLU, promoted anxiolytic effects in animals; (2) administration mirtazapine caused sedative effect to animals; (3) in the training session, the animals treated with BUS, MIR and FLU learned the task, on the other hand DZP group showed impairment in learning; (4) in the test session, animals treated with DZP, BUS, and MIR showed deficits in relation to discrimination between the enclosed arms, aversive versus non-aversive arm, demonstrating an impairment in memory, however, animals treated with FLU showed no interference in the retrieval of this memory; (5) acute stress did not interfere in locomotor activity, anxiety, or learning on the learning task, but induced impairment in retrieval memory, and the group treated with FLU did not demonstrated this deficit of memory . These results suggest that acute administration of drugs with anxiolytic and antidepressant activity does not interfere with the learning process this aversive task, but impair its retrieval, as well as the acute restraint stress. However, the antidepressant fluoxetine was able to reverse memory deficits promoted by acute stress, which may suggest that modulation, even acutely serotonergic neurotransmission, by selectively inhibiting the reuptake of this neurotransmitter, interferes on the process of retrieval of an aversive memory

Efeitos do tratamento com lítio na memória aversiva, comportamentos relacionados à ansiedade e depressão e na expressão de BDNF em ratos

Lithium (Li) is the first choice to treat bipolar disorder, a psychiatric illness characterized by mood oscillations between mania and depression. However, studies have demonstrated that this drug might influence mnemonic process due to its neuroprotector, antiapoptotic and neurogenic effects. The use of Li in the treatment of cognitive deficits caused by brain injury or neurodegenerative disorders have been widely studied, and this drug shows to be effective in preventing or even alleviating the memory impairment. The effects of Li on anxiety and depression are controversial and the relationship of the effects of lithium on memory, anxiety and depression remain unknown. In this context, this study aims to: evaluate the effects of acute and chronic administration of lithium carbonate in aversive memory and anxiety, simultaneously, using the plus maze discriminative avoidance task (PMDAT); test the antidepressant effect of the drug through the forced swimming test (FS) and analyze brainderived neurotrophic factor (BDNF) expression in structures related to memory and emotion. To evaluation of the acute effects, male Wistar rats were submitted to i.p. administration of lithium carbonate (50, 100 or 200 mg/kg) one hour before the training session (PMDAT) or lithium carbonate (50 or 100 mg/kg) one hour before the test session (FS). To evaluation of the chronic effects, the doses administered were 50 or 100 mg/kg or vehicle once a day for 21 days before the beginning of behavioral tasks (PMDAT and FS). Afterwards, the animals were euthanized and their brains removed and submitted to immunohistochemistry procedure to quantify BDNF. The animals that received acute treatment with 100 and 200 mg/kg of Li did not discriminated between the enclosed arms (aversive and non-aversive) in the training session of PMDAT, showing that these animal did not learned the task. This lack of discrimination was also observed in the test session, showing that the animals did not recall the aversive task. We also observed an increased exploration of the open arms of these same groups, indicating an anxiolytic effect. The same groups showed a reduction of locomotor activity, however, this effect does not seem to be related with the anxiolytic effect of the drug. Chronic treatment with Li did not promote alterations on learning or memory processes. Nevertheless, we observed a reduction of open arms exploration by animals treated with 50 mg/kg when compared to the other groups, showing an anxiogenic effect caused by this dose. This effect it is not related to locomotor alterations since there were no alterations in these parameters. Both acute and chronic treatment were ineffective in the FS. Chronic treatment with lithium was not able to modify BDNF expression in hippocampus, amygdala and pre-frontal cortex. These results suggest that acute administration of lithium promote impairments on learning in an aversive task, blocking the occurrence of memory consolidation and retrieval. The reduction of anxiety following acute treatment may have prevented the learning of the aversive task, as it has been found that optimum levels of anxiety are necessary for the occurrence of learning with emotional context. With continued, treatment the animals recover the ability to learn and recall the task. Indeed, they do not show differences in relation to control group, and the lack of alterations on BDNF expression corroborates this result. Possibly, the regimen of treatment used was not able to promote cognitive improvement. Li showed acute anxiolytic effect, however chronic administration 4 promoted the opposite effect. More studies are necessary to clarify the potential beneficial effect of Li on aversive memory

Efeito do alerta e valência emocional nos componentes de lembrança e familiaridade da memória episódica

The episodic memory system allows us to retrieve information about events, including its contextual aspects. It has been suggested that episodic memory is composed by two independent components: recollection and familiarity. Recollection is related to the vivid e detailed retrieval of item and contextual information, while familiarity is the capability to recognize items previously seen as familiars. Despite the fact that emotion is one of the most influent process on memory, only a few studies have investigated its effect on recollection and familiarity. Another limitation of studies about the effect of emotion on memory is that the majority of them have not adequately considered the differential effects of arousal and positive/negative valence. The main purpose of the current work is to investigate the independent effect of emotional valence and arousal on recollection and familiarity, as well as to test some hypothesis that have been suggested about the effect of emotion on episodic memory. The participants of the research performed a recognition task for three lists of emotional pictures: high arousal negative, high arousal positive and low arousal positive. At the test session, participants also rated the confidence level of their responses. The confidence ratings were used to plot ROC curves and estimate the contributions of recollection and familiarity of recognition performance. As the main results, we found that negative valence enhanced the component of recollection without any effect on familiarity or recognition accuracy. Arousal did not affect recognition performance or their components, but high arousal was associated with a higher proportion of false memories. This work highlight the importance of to consider both the emotional dimensions and episodic memory components in the study of emotion effect on episodic memory, since they interact in complex and independent way

Estampa temporal na memória de reconhecimento em estudantes universitários

Several studies have shown that there is a circadian modulation of explicit memory. This modulation can occur independently in each one of the mnemonic processes. The aim of this study was to evaluate the influence of time of training on short-term memory (STM) and long-term memory (LTM), using a recognition task. Moreover, a possible circadian modulation in retrieving was investigated when this process matched the acquisition hour (time stamp). The chronotype variable was also considered. Fifty-seven undergraduate students aging between 18 and 25 years (21,72 ± 2,14; 28 ♂) participated in this study. In the training phase (acquisition) the subjects heard a ten word list. Following this, they answered a recognition test to evaluate STM and one week later they answered a recognition test to evaluate LTM. In each chronotype, the subjects were divided in groups according to the training hour, part of them in the morning and the other in the afternoon. One week later some of the subjects in each group underwent LTM testing in the morning and others in the afternoon. When the subjects performances were analyzed together, independently of the chronotypes, a training hour effect was found in the LTM. The subjects trained in the afternoon had better performance. No time of day effect was found in the STM and in retrieving from the LTM. However, the morning types who were trained and tested in the same hour had a better performance in the LTM when compared to morning types trained and tested in different hours. This effect did not occur when the other chronotypes were analyzed. The circadian modulation seems to occur at least in two different ways. First, there is a circadian modulation in the acquisition/consolidation processes, with a better performance occuring in the afternoon. Secondly, there is a modulation in the retrieval mnemonic process, called time stamp phenomenon. This phenomenon, that occurred in the morning types, is showed for the first time in humans

Investigação da memória autobiográfica em idosos com demência de Alzheimer nas fases leve e moderada

Conselho Nacional de Desenvolvimento Científico e Tecnológico

Arquitetura com elevada taxa de processamento e reduzida largura de banda de memória para a estimação de movimento em vídeos digitais

Nowadays several electronics devices support digital videos. Some examples of these devices are cellphones, digital cameras, video cameras and digital televisions. However, raw videos present a huge amount of data, millions of bits, for their representation as the way they were captured. To store them in its primary form it would be necessary a huge amount of disk space and a huge bandwidth to allow the transmission of these data. The video compression becomes essential to make possible information storage and transmission. Motion Estimation is a technique used in the video coder that explores the temporal redundancy present in video sequences to reduce the amount of data necessary to represent the information. This work presents a hardware architecture of a motion estimation module for high resolution videos according to H.264/AVC standard. The H.264/AVC is the most advanced video coder standard, with several new features which allow it to achieve high compression rates. The architecture presented in this work was developed to provide a high data reuse. The data reuse schema adopted reduces the bandwidth required to execute motion estimation. The motion estimation is the task responsible for the largest share of the gains obtained with the H.264/AVC standard so this module is essential for final video coder performance. This work is included in Rede H.264 project which aims to develop Brazilian technology for Brazilian System of Digital Television

Processos aleatórios não-markovianos: perfis de memória

One of the mechanisms responsible for the anomalous diffusion is the existence of long-range temporal correlations, for example, Fractional Brownian Motion and walk models according to Elephant memory and Alzheimer profiles, whereas in the latter two cases the walker can always "remember" of his first steps. The question to be elucidated, and the was the main motivation of our work, is if memory of the historic initial is condition for observation anomalous diffusion (in this case, superdiffusion). We give a conclusive answer, by studying a non-Markovian model in which the walkers memory of the past, at time t, is given by a Gaussian centered at time t=2 and standard deviation t which grows linearly as the walker ages. For large widths of we find that the model behaves similarly to the Elephant model; In the opposite limit (! 0), although the walker forget the early days, we observed similar results to the Alzheimer walk model, in particular the presence of amnestically induced persistence, characterized by certain log-periodic oscillations. We conclude that the memory of earlier times is not a necessary condition for the generating of superdiffusion nor the amnestically induced persistence and can appear even in profiles of memory that forgets the initial steps, like the Gausssian memory profile investigated here.

Os 'troncos velhos' e os 'quilombinhos': memória genealógica, território e afirmação étnica em Boa Vista dos Negros (RN)

Durante a pesquisa visando à elaboração do relatório antropológico de Boa Vista dos Negros, comunidade quilombola do Seridó (RN), verificamos que o grupo conserva uma longa memória genealógica e que, apesar das mudanças ocorridas na organização econômica e social do grupo, as principais formas de solidariedade foram mantidas. Queremos, aqui, refletir sobre a importância da noção de família, a transmissão dos nomes e da memória genealógica na definição do território quilombola. Na ocasião, serão analisados como os elementos da cultura tradicional são ressignificados ao longo do processo de afirmação étnica

Mito e memória na construção de uma identidade local

CAVIGNAC, J. A. Mito e memória na construção de uma identidade local. Organon, Rio Grande do Sul, v. 21, n.42, jan./jun. 2007. Disponível em: . Acesso em:

Perfil imunológico associado à susceptibilidade, resistência e cura na infecção por Leishmania ( Leishmania ) infantum

Visceral Leishmaniasis (VL) is endemic in Brazil and the northeast region had the highest incidence of the disease , despite, in the last 30 years, it has spread to all geographic regions of the country. Leishmania infantum is the m ain etiological agent of VL in Latin America, Europe and North Africa. However, not all infected individuals develop the disease; in fact, the majority present spontaneous re solution of infection without symptoms. The evaluation of the immunological profil e has been mostly conducted stimulating, with Leishmania spp. antigen, peripheral blood mononuclear cells isolated from subjects with VL. These studies showed that VL patients had an inhibition of both, lymphocyte proliferation and proinflammatory response to Leishmania spp. antigen. Our study aimed to evaluate the immune response in active LV, cured post treatment and asymptomatic infection. To reach this aim, we analyzed immunophenotypic features related to activation, Treg and memory lymphocytes, by flow cytometry, as well as, evaluation of cytokine production, in ex vivo or in whole blood culture. In active VL volunteers, a longitu dinal study was conducted with reassessment at 4 and 14 months after clinical cure. The control group included individuals th at live d in endemic region and were either Positive Control, consisting of individuals with positive anti - L eishmania spp. serology and/or positive PCR for Leishmania  spp. and Negative Control composed by individuals with negative anti - Leishmania antibodie s serology and negative PCR for Leishmania . During VL, CD4 lymphocytes showed greater activation and memory profile s and were the major source of cytokines in culture when compared to CD8 lymphocytes , and these were not Leishmania specific. There were act ivated lymphocytes during VL (CD4 + CD69 + :4.9%) when compared to control groups, Positive (CD4 + CD69 + :1.96%, p=0.0045) and Negative (CD4 + CD69 + :1.35%, p=0.006), on the other hand, this was non - specific activation. The lymphocyte activation profile remain ed el evated even 14 months post treatmen t. A fter clinical cure , the activation was Leishmania specific (CD4 + CD25 + absence of SLA: 8.4%, and presence of SLA: 10.7% p=0.0279). CD8 + CD25 + lymphocytes were able to produce Leishmania specific IFN - γ in both, Positive Controls (absence of SLA 5.2% and presence of SLA: 9.5%, p=0.0391) and Cured 4 month (absence of SLA: 3.9%; presence of SLA: 10.7% p=0.0098). Whole blood culture cells, of VL patients, were able to produce IFN - γ, by SLA stimulation (absence of SLA: 28.0 pg ∕mL, and presence: 44.3 pg∕mL p=0.0020) as well as recovered groups (absence of SLA 2.3 pg∕mL and presence of SLA 139.8 pg∕mL, p=0.0005). However, the high level of IL - 10 seem ed to inhibit pro - inflammatory activity of IFN - γ and TNF - α during symptomatic dis ease . Unlike other pro - inflammatory cytokines, active VL group d id not produce Leishmania specific IL - 2 (absence of SLA 2.4 pg∕mL and presence of SLA: 2.6 pg∕mL). Based on these data we conclude that the restoration of lymphocyte activation and decreased i n IL - 10 Leishmania specific production were related to a protective immune profile.

A cafeína como agente modulador do ciclo atividade-repouso e memória em saguis (Callithrix jacchus)

Caffeine is the most consumed psychostimulant, with effects on attention, memory, and arousal. But when this substance is ingested near to bedtime there is a decrease on sleep, interfering on mnemonic processes. So, our ain was to investigate how the caffeine ingested near to sleep onset acts on sleep and memory in marmosets. We used 16 adult marmosets, single housed, in a 12:12h light-dark cycle. For registering locomotor activity were used two kinds of sensors. The gyroscope sensor registers activity each 30 sec and detects motion with good accuracy. Because of this we used this sensor for detecting nocturnal activity. The second sensor was based on infrared and accumulates activity each 5 min and it’s not able to detect nocturnal activity, just diurnal activity. We also used camera for registering Rest phase of one marmoset. For the cognitive task, the animals needed to learn a rewarded context (CR) when compared to a non-rewarded context CNR). This experiment comprises 5 phases: 1) Two days of habituation to apparatus; 2)Training for 8 days; 3) oral administration of caffeine (10 mg/kg) or placebo administration ±1h before sleep onset, for 8 days, with marmosets receiving placebo or caffeine; 4) retraining to apparatus and after that, placebo administration (placebo group-GP), or caffeine administration (with continuous group-GC and acute groupGA); 5) Test, for evaluating learning to CR. The sessions were filmed and each one had 8 min of duration. At 7 am started the habituation, training and test sessions, and at 3:15 pm started retraining. The results for gyroscope sensor showed that there was coincidence of 68,57% with nocturnal register of the cameras. Then, the gyroscope sensors detected nocturnal activity for all experimental groups Moreover, when compared sensor gyroscope with sensor based on infrared, was observed that both sensor presented similarity on patterns of activity curve. When we observed the effects of caffeine on Activity-Rest Cycle in GP, GA and GC, is possible to see that that gyroscope sensors and based on infrared presented only intra group differences. As behavioral results, the marmosets learned to discriminate CR when compared to CNR. Moreover, GP presented deficits on memory recall during the test, and GA increased the memory recall, when both were compared to GP. We concluded that the marmosets were able to learning the cognitive task and that the caffeine ingested near to sleep onset acts modulating memory in these animals. Moreover the gyroscope sensor can be used as alternative tool for investigating nocturnal activity. Then, the utilization of this non-invasive device allows marmosets exhibit their behavior within the laboratory conditions as natural as possible.

Os fios da memória: presença afro-brasileira em Acari no tempo do algodão

La historia de la expansión colonial en los sertónes del Seridó del siglo XVIII y el asentamiento de los primeros pobladores alrededor de las haciendas de ganado y más tarde de la cultura de algodón, oculto la presencia afrodescendiente que ya regia esa vasta región. Por otro lado, la esclavitud se observaba como un fenómeno de segunda prioridad y leve, por el hecho de tener un número de esclavos muy reducido en relación al litoral azucarero; sin embargo no se puede minimizar las marcas que dejaron más de tres siglos de dominación colonial, pues la violencia simbólica en su descripción aun existe. Este trabajo tiene como objetivo reflexionar sobre las causas y consecuencias de la extinción precoz de la presencia afro-brasileña y de la invisibilidad de los núcleos familiares en el municipio de Acari. A través de las memorias de las familias Nunes, Inácio y Pereira, antiguos moradores del Saco de los Pereira y de las familias Pedros, Paula, Higinos y Félix outroras moradores de las haciendas de la región, pretendemos reflexionar sobre las actividades de sobrevivencia, las relaciones de trabajo, la propiedad de la tierra y los robos ocurridos en los siglos XIX – XX, así como mostrar la importancia de las tradiciones familiares en la elaboración de los discursos sobre el pasado y de las variadas identidades. La metodología utilizada durante la investigación, mantuvo como foco las entrevistas que contemplan historias de la vida y las memorias de nuestros interlocutores, en particular los afrodescendientes. Los relatos colocan una luz sobre las vivencias en el período algodonero, los oficios realizados en las haciendas (vaquero, bordado, culinaria, losa) donde se muestra la importancia de las familias negras para entender el escenario Acarienses. También fotos y documentos cartoriáles que ayudaron a componer las historias de vida. El estudio revela la presencia de muchas familias negras vinculadas a las haciendas, demostrando que existe otra versión de la historia local, teniendo como protagonistas aquellos cuya memoria fue silenciada y quedando así marcados por el estigma de la esclavitud.

O paradoxo da superdifusão de uma caminhada aleatória com memória exponencial

The random walk models with temporal correlation (i.e. memory) are of interest in the study of anomalous diffusion phenomena. The random walk and its generalizations are of prominent place in the characterization of various physical, chemical and biological phenomena. The temporal correlation is an essential feature in anomalous diffusion models. These temporal long-range correlation models can be called non-Markovian models, otherwise, the short-range time correlation counterparts are Markovian ones. Within this context, we reviewed the existing models with temporal correlation, i.e. entire memory, the elephant walk model, or partial memory, alzheimer walk model and walk model with a gaussian memory with profile. It is noticed that these models shows superdiffusion with a Hurst exponent H > 1/2. We study in this work a superdiffusive random walk model with exponentially decaying memory. This seems to be a self-contradictory statement, since it is well known that random walks with exponentially decaying temporal correlations can be approximated arbitrarily well by Markov processes and that central limit theorems prohibit superdiffusion for Markovian walks with finite variance of step sizes. The solution to the apparent paradox is that the model is genuinely non-Markovian, due to a time-dependent decay constant associated with the exponential behavior. In the end, we discuss ideas for future investigations.

Cartilha do silêncio: sob o signo da modernidade e da memória

This research starts from the presupposition that Cartilha do Silêncio(1997), a novel by the Brazilian writer Francisco Dantas, has a double articulated shift. One of the moves is towards the modern experience, with the idea that modernity is filled with contraries, as remarked by Nietzsche; the other is linked to the livelihoods ashore on traditional experiences, which encompasses the notion of memory as individual and collective ownership. The aim here is to analyze such perspective, social and critical issues within the characters' life stories that regards the calling of past as clear example that tradition is not gone, though modern life presents its own signs. Such dynamics gives to the plot a paradoxal feature. This work is mainly grounded on Marshall Berman' s thoughts in All That Is Solid Melts Into Air: The Experience of Modernity (1982) as well as on Antoine Compagnon'sFive Paradoxes of Modernity (1994). Assuming that Francisco Dantas' Novel is set as a split narrative, outcome of social memory originated on individual experiences aside social process and patriarchal family, this research brings into play the concept of memory by Jacques Le Goff in History and Memory (1992) along EcléaBosi's study in Memória e Sociedade: lembranças de velhos (1979). Keen to check how Cartilha do silêncio adjoins modern livelihoods with aesthetics order, the method articulates text and context, literary and social life, according to Antonio Candido'sLiteratura e Sociedade (1965). Thus, after reading the novel, it is possible to notice how the identity of the characters are built throughout the plot and it is also kept against settling on its social context during the transition from patriarchal tradition to modernity, creating a taut mood between both registries.

A céu aberto: garimpando a memória e a identidade dos mineradores de Brejuí

The Brejuí Mine, situated in the municipality of Currais Novos (RN), was responsible for the local economy growth between the years of 1943 and 1990, causing the interest of miner's working class to its urban center. After the end of scheelita extraction, the mine became a "theme park", in 2004. The company left its mark on the city by building several monuments in reference to the mining activity and the company's founder, Tomaz Salustino. However, there are no written records that describe such activities on its early years, nor their pioneers in the mining subject. Therefore, it is important to investigate the miner’s memories and ask if, along the more than five decades that lasted the heyday of mining, an identity linked to the profession was built; the extent to which workers make reference to this history as membersand what are the symbols that are being triggered in the development of a working class identity. Further, I investigate what memory was mad concerning the pioneers, mostly farmers attracted by miner work. To do so, I make use of audiovisual archival resources and oral records of the interlocutors that were filmed in the preparations of the documentary “Lembranças de velhos garimpeiros". We have noticed that, in the script of the official history of the Brejuí Mine, the "boss" figure overlaps the workers and that the forms patronage, originated from the rural world, followed punctuating the social relations in the mine. Today, with the resumption of the mining activity, it is possible that the strengthening of the working class and the Seridó miner identity desire reborn.