992 resultados para 143-868A


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Albumin, the most abundant protein components of blood plasma, is synthesized and secreted by liver cells in vertebrates. Recently, it was demonstrated that frog Bombina maxima albumin is also expressed in skin. Both B. maxima albumins from skin and serum (BmA-skin and BmAserum) have similar biochemical characteristics except that the former contains haem b. Present studies showed that BmA-skin exhibited cytotoxic activity on H9 and C8166 cells. Pretreated with hemin to induce erythroid differentiation, K562 cells lost their resistance to cytotoxicity of BmAskin. After treating cells with BmA-skin for 48 h, 50 percentage cytotoxic concentrations (CC50) of BmA-skin on H9, C8166 and hemin-treated K562 cells were 1.31±0.09, 1.59±0.08 and 2.28±0.06 μM, respectively. The cell death induced by BmA-skin was mediated by apoptosis of the tested cell lines, as demonstrated by nuclear morphological changes, DNA fragmentation and DNA hypodiploidy of apoptosis cells. At BmA-skin concentration of 2 μM, 27.3%, 19.7% and 17.8% of H9, C8166 and hemin-treated K562 cells were found to be apoptotic. In contrast, BmA-serum possessed no cytotoxic and apoptosis-inducing activity on all the cell lines tested, even with concentration used up to 15 μM. These results indicated that bound haem b in BmA-skin contributed significantly to its cytotoxic and apoptosis-inducing activity on the cell lines assayed.

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采用ICP刻蚀的方法,在SOI材料上制作出了中心波长为1.5509μm、信道间隔为200GHz的5×5阵列波导光栅(AWG).测试中心波长与设计值相差0.28nm,测试波长间隔与设计值相差在0.02nm之内,相邻信道串扰接近10dB,信道插入损耗均匀性为0.7dB,测试结果表明该器件能够初步达到分波功能.

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用金属有机物气相外延设备,在氮化镓/蓝宝石复合衬底上快速外延生长铟镓氮薄膜,并对其进行了X射线三晶衍射、光致发光、反射光谱及霍尔测量等实验测试。确定该薄膜为单晶,其中In组分可以从0增加到0.26;在光致激发下发光光谱为单峰,且峰值波长在360~555nm范围内可调;其发光机理被证实为膜内载流子经带隙跃迁而直接复合;并具有很高的电子浓度。但InGaN薄膜的结晶质量却随着In含量的增加而变差。

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分析了GaAs/AlGaAs半导体多量子阱光开关器件的室温激子吸收行为及光调制特性,优化设计了多量子阱结构,研制出常通型和常关型两种类型光开关器件,并对器件的光调制特性进行了测量与研究。实验得出的结构与理论计算相符合。

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于2010-11-23批量导入

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于2010-11-23批量导入

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于2010-11-23批量导入

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The tunable ridge waveguide distributed Bragg reflector (DBR) lasers designed for wavelength-division-multiplex (WDM) communication systems at 1.55 um by using selective area growth (SAG) is reported. The threshold current of the DBR laser is 62mA and the output power is more than 8mW. The isolation resistance between the active region and the Bragg region is 30K Ohm. The total tuning range is 6.5nm and this DBR laser can provide 6 continuous standard WDM channels with 100GHz channel spacing; in the tuning range, the single mode suppression ratio (SMSR) is maintained more than 32dB and the maximum output power variation is less than 3dB.

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The behaviors of lateral propagating modes in the aperture and the oxidized regions are investigated numerically for selectively oxidized vertical-cavity surface-emitting lasers (VCSELs). The results show that the lateral propagating modes in the oxidized region are greatly affected by the oxide layer due to its low index, the modes are divergence for the VCSELs with sufficient thick double oxide layers. So the coupling between the modes in the aperture and oxidized regions is very weak, and we can expect that the lateral spontaneous emission is greatly affected in this case. Ignoring the contribution of the lateral spontaneous emission, we calculate spontaneous emission factor by counting the total number of the guided modes in selectively oxidized VCSELs with double oxide layers. The results agree very well with the reported measurements and are inversely proportional to the lateral index step.

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分析并比较现有网络安全态势评估方法,从网络拓扑结构出发,引入图论算法和数据挖掘的聚类方法,提出一种针对分布式系统的安全态势评估方法.利用图聚类算法生成分布式系统网络连通图的聚类结果,计算拓扑完整性参考值,作为衡量分布式系统拓扑完整程度的指标,进行安全态势的量化分析.根据该方法实现低人为干预的安全态势评估原型系统,使用网络仿真工具验证了算法正确性和适用性.

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提出一种新的无线局域网认证密钥协商协议,可以提供双方相互认证及密钥确认.该协议在密钥设置上基于挑战响应协议和KAS方案,在密钥预分配上基于Diffie-Hellman协议,可以提供完美前向安全性,抵抗被动攻击、字典攻击、中间人攻击、假冒攻击等.并对协议的计算代价和通信代价进行分析.

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人类长期且大量的使用药品及个人护理用品(PPCPs),使其持续不断地输入环境,导致它们在环境中的残留浓度日益增加,并对人类和其他生物产生了不良的生态毒理效应。本文以四种典型PPCPs(对乙酰氨基酚、土霉素、三氯生、佳乐麝香)为研究对象,采用常规的毒理学实验方法,研究了它们对小麦种子发芽、根伸长的急性毒性,以及低浓度长期暴露对小麦幼苗叶绿素(CHL)、可溶性蛋白(SP)、过氧化物酶(POD)和超氧化物歧化酶(SOD)等生理生化特性的影响。由于PPCPs具有生态风险性,而传统的水处理工艺却不能有效的将这些物质去除。因此,开发更多的新技术以强化处理PPCPs,使它们在环境中残留的浓度尽可能降低是一项很重要的工作。本研究以零价铁处理有机物的原理为依据,自制铁铜双金属复合物,研究了其对PPCPs溶液的处理效果及影响因素,并对整个过程中的反应机理进行了初探。实验结果表明: 实验浓度下,四种PPCPs与小麦根长及芽长抑制率之间具有良好的剂量-效应关系。根据线性回归方程计算的EC50可知,不同种类的PPCPs对小麦种子发芽的毒性大小不同,而且小麦的芽及根部对不同PPCPs胁迫的敏感部位不同。对乙酰氨基酚、土霉素、三氯生对小麦根长的抑制较芽长显著,它们的EC50分别为668.8 mg/L、34.7 mg/L和147.8mg/L;而佳乐麝香对小麦芽长的抑制更为显著,EC50为143.4 mg/L。实验浓度范围内,四种PPCPs对小麦种子的发芽率均没有显著的抑制作用。四种PPCPs暴露21天后,2.8~22.4 mg/L对乙酰氨基酚、0.15~2.4 mg/L土霉素以及0.2~3.0 mg/L三氯生和佳乐麝香显著抑制小麦叶片/根部中的CHL和SP,而且使POD和SOD酶活性降低或上升,从而打破抗氧化系统的动态平衡;暴露21天内对CHL、SP、POD和SOD等含量在不同PPCPs胁迫下的变化趋势具有各自的特点。 以零价铁降解有机物的原理为依据,自制了铁铜双金属复合体系,以强化处理PPCPs溶液。电子扫描显微镜(SEM)及X-衍射(XRD)分析得出铁铜复合物(FeCu/Cu2O)由Fe、Cu及Cu2O组成。与零价铁相比,FeCu/Cu2O显著提高了对PPCPs的去除效果,主要是由于Cu的存在扩大了反应的电极电位差,即增加了Fe-Cu之间的外加阳极电流,从而加速Fe的阳极溶解,产生更多的高活性物质以更好的去除PPCPs。溶解氧是影响FeCu/Cu2O对PPCPs处理效果的重要因素,它们可以在FeCu/Cu2O表面获得电子后生成O2-,形成类Fenton体系。此外,由于Cu2O的光化学活性,光照也可以强化FeCu/Cu2O对PPCPs的处理效果。不同的反应条件下体系内的pH值及自由基的产生量不同。根据实验现象及相关的经典理论,本文对FeCu/Cu2O处理PPCPs的作用机理进行了初探,认为整个反应过程包括Fe的还原反应、Cu2O的光催化反应、Fe-Cu微电解反应以及絮凝过程等。

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沈阳是东北地区重要的老工业基地,也是该地区的交通枢纽。本文在对沈阳市进行广泛调查并采集苔藓植物标本的基础上,系统地研究了沈阳市苔藓植物的种类构成、区系、分布格局及其与环境因子的关系,同时利用苔藓植物对沈阳市的大气质量进行了指示。得出的主要结论有: (1)对882份苔藓植物标本进行鉴定,共记录苔藓植物30科67属143种及变种,其中包括苔类(含角苔)9科11属14种,藓类21科56属128种1变种。在记录中发现辽宁省新记录属1个,新纪录种36种,东北新纪录种18种,主要以丛藓科(Pottiaceae)和真藓科(Bryaceae)等地面生藓类为主。 (2)将研究区苔藓植物划分为10个区系成分,其中以北温带分布占据绝对优势,其次为东亚分布和中国特有分布,具强烈的温带性质。通过相似性系数的计算,表明沈阳市苔藓植物区系与鞍山市和抚顺市的亲缘性较大;与上海市和杭州市亲缘性较远。 (3)应用TWINSPAN和DCA,以样点为对象,苔藓植物的重要值为指标,对研究区苔藓植物分布格局进行了分析。结果将样点划分为三个组,从组一到组三,物种丰富度和苔藓盖度逐渐增加。分类与排序的结果与样点的实际物种分布特点基本一致。 (4)采用CCA对沈阳市35种主要地面苔藓植物与环境因子间的关系进行了分析。根据环境因子的影响,将沈阳市主要地面苔藓植物分为两类。同时发现土壤含水量、乔木层郁闭度和人为干扰是影响沈阳市苔藓植物分布的主要因素。 (5)采用大气净度指数法(IAP)和重金属元素(Mn, Fe, Cu, Cr和Pb)化学分析法对沈阳市的大气质量进行了监测。结果表明市区污染远重于郊区,市区西部污染重于东部,南部重于北部;各区以Pb污染最为严重,值得重视。同空气污染指数(API)进行比较,发现IAP与Cu、Pb和API之间具显著负相关性,说明随着重金属含量和API值的升高,IAP值降低,即苔藓植物的种类和盖度下降。以IAP值为自变量,API值为因变量,建立了回归方程y=-0.914x+101.849,进一步说明了IAP值与API值之间的关系。