Síndrome de Guillain-Barré: epidemiologia, prognóstico e fatores de risco

Introduction. Guillain-Barré syndrome (GBS) is an immune-mediated polyneuropathy and the principal cause of acute neuromuscular paralysis. The most prominent GBS subtypes are: acute inflammatory demyelinating polyneuropathy (AIDP), acute motor axonal neuropathy (AMAN), acute motor-sensory axonal neuropathy (AMSAN) and Fisher syndrome (FS). Differences in geographical distribution of variants have been reported. In Brazil, there are few studies describing the characteristics of GBS, but none on the frequency of GBS variants and their clinical manifestations. Infection-induced aberrant immune response resulting from molecular mimicry and formation of cross-reacting antibodies, contribute to complement activation. Functional biallelic polymorphism in immunoglobulin receptors that influence the affinity of IgG subclasses and the type of immune response have been described, suggesting genetic susceptibility to developing disease. It remains unclear whether individuals carrying different FCGR alleles have differential risk for GBS and⁄or disease severity. The goals of this study were: (1) To characterize GBS and describe the clinical findings in a cohort of patients with GBS from the state of Rio Grande do Norte, Brazil; (2) to determine whether polymorphism in FCGR were associated with development of GBS, and (3) to tease out whether the global gene expression studies could be a tool to identify pathways and transcriptional networks which could be regulated and decrease the time of disease. Methods. Clinical and laboratory data for 149 cases of GBS diagnosed from 1994 to 2013 were analyzed. Genomic DNA and total RNA were extracted from whole blood. Antigangliosides antibodies were determined in the sera. In addition, we also assessed whether FCGR polymorphism are present in GBS (n=141) and blood donors (n=364), and global gene expressions were determined for 12 participants with GBS. Blood samples were collected at the diagnosis and post-recovery. Results. AIDP was the most frequent variant (81.8%) of GBS, followed by AMAN (14.7%) and AMSAN (3.3%). The incidence of GBS was 0.3 ⁄ 100,000 people for the state of Rio Grande do Norte and cases occurred at a younger age. GBS was preceded by infections, with the axonal variant associated with episodes of diarrhea (P = 0.025). Proximal weakness was more frequent in AIDP, and distal weakness predominant in the axonal variant. Compared to 42.4% of cases with AIDP (P<0.0001), 84.6% of cases with the axonal variant had nadir in <10 days. Individuals with the axonal variant took longer to recover deambulation (P<0.0001). The mortality of GBS was 5.3%. A worse outcome was related to an axonal variant (OR17.063; P=0.03) and time required to improve one point in the Hughes functional scale (OR 1.028; P=0.03). The FCGR genotypes and allele frequencies did not differ significantly between the patients with GBS and the controls (FCGR2A p=0.367 and FCGR3A p=0.2430). Global gene expression using RNAseq showed variation in transcript coding for protein isoforms during acute phase of disease. Conclusions. The annual incidence of GBS was 0.3 per 100,00 and there was no seasonal pattern. A predominance of the AIDP variant was seen, and the incidence of the disease decreased with age. The distribution of weakness is a function of the clinical variants, and individuals with the axonal variant had a poorer prognosis. Early diagnosis and variant identification leads to proper intervention decreasing in long-term morbidity. FCGR polymorphisms do not seem to influence susceptibility to GBS in this population. This study found deregulated genes and signs of transcriptional network alterations during the acute and recovery phases in GBS. Identification of pathways altered during disease might be target for immune regulation and with potential to ameliorate symptoms.

Proposta de válvula bypass para controle de velocidade de PIGs instrumentados: protótipo e simulação em bancada

In the Oil industry, oil and gas pipelines are commonly utilized to perform the transportation of production fluids to longer distances. The maintenance of the pipelines passes through the analysis of several tools, in which the most currently used are the pipelines inspection cells, popularly knowing as PIG. Among the variants existing in the market, the instrumented PIG has a significant relevance; acknowledging that through the numerous sensors existing in the equipment, it can detect faults or potential failure along the inspected line. Despite its versatility, the instrumented PIG suffers from speed variations, impairing the reading of sensors embedded in it. Considering that PIG moves depending on the speed of the production fluid, a way to control his speed is to control the flow of the fluid through the pressure control, reducing the flow rate of the produced flow, resulting in reduction of overall production the fluid in the ducts own or with the use of a restrictive element (valve) installed on it. The characteristic of the flow rate/pressure drop from restrictive elements of the orifice plate is deducted usually from the ideal energy equation (Bernoulli’s equation) and later, the losses are corrected normally through experimental tests. Thus, with the objective of controlling the fluids flow passing through the PIG, a valve shutter actuated by solenoid has been developed. This configuration allows an ease control and stabilization of the flow adjustment, with a consequent response in the pressure drops between upstream and downstream of the restriction. It was assembled a test bench for better definition of flow coefficients; composed by a duct with intern diameter of four inches, one set of shutters arranged in a plate and pressure gauges for checking the pressure drop in the test. The line was pressurized and based on the pressure drop it was possible to draw a curve able to characterize the flow coefficient of the control valve prototype and simulate in mockup the functioning, resulting in PIG speed reduction of approximately 68%.

Um controlador dual subótimo com fator de peso variável no tempo

This work concerns a refinement of a suboptimal dual controller for discrete time systems with stochastic parameters. The dual property means that the control signal is chosen so that estimation of the model parameters and regulation of the output signals are optimally balanced. The control signal is computed in such a way so as to minimize the variance of output around a reference value one step further, with the addition of terms in the loss function. The idea is add simple terms depending on the covariance matrix of the parameter estimates two steps ahead. An algorithm is used for the adaptive adjustment of the adjustable parameter lambda, for each step of the way. The actual performance of the proposed controller is evaluated through a Monte Carlo simulations method.

Os possessivos de segunda pessoa em cartas de leitores de jornais brasileiros dos séculos XIX e XX

Based on the theoretical and methodological presuppositions of the theory of language variation and change (cf. WEINREICH; LABOV; HERZOG, 2006 [1968]), it is described and analyzed in this article the process of variation/change concerning the second person possessive pronouns in letters from readers of Brazilian newspapers from the XIX and XX centuries. These letters feature a portrait of the Brazilian press from the South (Santa Catarina), Southeast (Rio de Janeiro) and Northeast (Bahia and Rio Grande do Norte) regions in each century and are part of the Project for Brazilian Portuguese History‘s (PHPB) printed common minimal corpus. The point of departure of this work is the idea that the use of variant forms of expressing second person possessive pronouns – teu and seu – results from the interaction characterizing the varied social roles performed by the letters‘ senders. Arranging communicative units, which gather elements/features denoting time and space, conditioned and determined by socio-historical and cultural aspects, the readers‘ letters, turn out to be a promising research field under the light of this paper. More specifically, In the row of presented results in studies about the pronominal system in the diachroneity of/in Brazilian Portuguese (PB) (FARACO, 2002; LORENGIAN-PENKAL, 2007; CALLOU; LOPES, 2003; LOPES; DUARTE, 2003; MENON, 2005; ARDUIN; COELHO, 2006; LOPES, 2009; MARCOTULIO, 2010), the results featured in here point at different usages of the possessives, noticing the coexistence of the forms teu/tua and seu/sua strongly conditioned by the socio-discursive nature of the readers‘ letters in the course of the centuries and through different regions.

Os possessivos de segunda pessoa em cartas de leitores de jornais brasileiros dos séculos XIX e XX

Based on the theoretical and methodological presuppositions of the theory of language variation and change (cf. WEINREICH; LABOV; HERZOG, 2006 [1968]), it is described and analyzed in this article the process of variation/change concerning the second person possessive pronouns in letters from readers of Brazilian newspapers from the XIX and XX centuries. These letters feature a portrait of the Brazilian press from the South (Santa Catarina), Southeast (Rio de Janeiro) and Northeast (Bahia and Rio Grande do Norte) regions in each century and are part of the Project for Brazilian Portuguese History‘s (PHPB) printed common minimal corpus. The point of departure of this work is the idea that the use of variant forms of expressing second person possessive pronouns – teu and seu – results from the interaction characterizing the varied social roles performed by the letters‘ senders. Arranging communicative units, which gather elements/features denoting time and space, conditioned and determined by socio-historical and cultural aspects, the readers‘ letters, turn out to be a promising research field under the light of this paper. More specifically, In the row of presented results in studies about the pronominal system in the diachroneity of/in Brazilian Portuguese (PB) (FARACO, 2002; LORENGIAN-PENKAL, 2007; CALLOU; LOPES, 2003; LOPES; DUARTE, 2003; MENON, 2005; ARDUIN; COELHO, 2006; LOPES, 2009; MARCOTULIO, 2010), the results featured in here point at different usages of the possessives, noticing the coexistence of the forms teu/tua and seu/sua strongly conditioned by the socio-discursive nature of the readers‘ letters in the course of the centuries and through different regions.

Variabilidade genética de Leishmania spp. circulantes entre seres humanos e cães e infecção de flebotomíneos em áreas endêmicas para as leishmanioses no Rio Grande do Norte

Leishmania infantum is the main etiologic agent of visceral leishmaniasis in the New World. The pattern of distribution of leishmaniasis has changed substantially and has presented an emerging profile within the periphery of the Large Urban Centers. Leishmania infection can compromise skin, mucosa and viscera. Only 10% of the individuals infected develop the disease and 90% of human infection is asymptomatic. The main factors involved in the development of the disease are the host immune response, the vector’s species and the parasite’s genetic content. The sequencing of Leishmania isolated seeks to increase the understanding of the symptoms of individuals. The aim of this study was to evaluate the genetic diversity of circulating Leishmania strains among humans, and symptomatic and asymptomatic, and dogs from endemic areas of Rio Grande do Norte State and analyze sandflies from endemic areas for cutaneous and visceral disease. The genetic variability was evaluated by the use of markers hsp70 , ITS1 and a whole genome sequencing was also carried out. The amplified hsp70 and ITS1 of samples were analyzed and assembled using a Phred / Phrap package. The dendograms were constructed using the same methodology, but adding 500 bootstraps, followed by inferences on the relationships between Leishmania variants. The sequences of the 20 Brazilian isolates were mapped to the reference genome L. infantum JPCM5, using the Bowtie2 program and the identification of 36 contigs. The information of the valid SNPs were used in the PCA. SNPs were visualized by Geneious 7.1 and IGV. The genome annotations were transferred to their respective chromosomes and displayed on Geneious. The matching sequences of all chromosomes were aligned using Mauve. The phylogenetic trees were calculated according to maximum likelihood and JTT models. Sandflies were analyzed by PCR for the identification of Leishmania infection, a blood meal source and GAPDH sand fly. As a result, hsp70 and ITS1 were not capable of identifying genetic variability among human isolates from symptomatic and asymptomatic, and dogs. The complete sequencing of the 20 Brazilian isolates revealed a strong similarity between the circulating Leishmania strains in Rio Grande do Norte. The isolates collected in the city of Natal from humans and canines remained grouped in all analyzes, suggesting that there is genotypic and geographic proximity among the isolates. The isolated samples in the 1990s had a higher genotypic diversity when compared to freshly isolated samples. All isolates presented 36 chromosomes with variable ploidy among them, no correlation was found between the number of amastina genes copies, gp63, A2 and SSG with such clinic forms. In general, we did not find correlation between symptomatic and asymptomatic clinical forms and the gene content of the Brazilian isolates of Leishmania. 34,28% of the sandflies collected in the upper west region were L. longipalpis and the main sources of blood meal were humans, dogs and chickens.

Variabilidade genética de Leishmania spp. circulantes entre seres humanos e cães e infecção de flebotomíneos em áreas endêmicas para as leishmanioses no Rio Grande do Norte

Leishmania infantum is the main etiologic agent of visceral leishmaniasis in the New World. The pattern of distribution of leishmaniasis has changed substantially and has presented an emerging profile within the periphery of the Large Urban Centers. Leishmania infection can compromise skin, mucosa and viscera. Only 10% of the individuals infected develop the disease and 90% of human infection is asymptomatic. The main factors involved in the development of the disease are the host immune response, the vector’s species and the parasite’s genetic content. The sequencing of Leishmania isolated seeks to increase the understanding of the symptoms of individuals. The aim of this study was to evaluate the genetic diversity of circulating Leishmania strains among humans, and symptomatic and asymptomatic, and dogs from endemic areas of Rio Grande do Norte State and analyze sandflies from endemic areas for cutaneous and visceral disease. The genetic variability was evaluated by the use of markers hsp70 , ITS1 and a whole genome sequencing was also carried out. The amplified hsp70 and ITS1 of samples were analyzed and assembled using a Phred / Phrap package. The dendograms were constructed using the same methodology, but adding 500 bootstraps, followed by inferences on the relationships between Leishmania variants. The sequences of the 20 Brazilian isolates were mapped to the reference genome L. infantum JPCM5, using the Bowtie2 program and the identification of 36 contigs. The information of the valid SNPs were used in the PCA. SNPs were visualized by Geneious 7.1 and IGV. The genome annotations were transferred to their respective chromosomes and displayed on Geneious. The matching sequences of all chromosomes were aligned using Mauve. The phylogenetic trees were calculated according to maximum likelihood and JTT models. Sandflies were analyzed by PCR for the identification of Leishmania infection, a blood meal source and GAPDH sand fly. As a result, hsp70 and ITS1 were not capable of identifying genetic variability among human isolates from symptomatic and asymptomatic, and dogs. The complete sequencing of the 20 Brazilian isolates revealed a strong similarity between the circulating Leishmania strains in Rio Grande do Norte. The isolates collected in the city of Natal from humans and canines remained grouped in all analyzes, suggesting that there is genotypic and geographic proximity among the isolates. The isolated samples in the 1990s had a higher genotypic diversity when compared to freshly isolated samples. All isolates presented 36 chromosomes with variable ploidy among them, no correlation was found between the number of amastina genes copies, gp63, A2 and SSG with such clinic forms. In general, we did not find correlation between symptomatic and asymptomatic clinical forms and the gene content of the Brazilian isolates of Leishmania. 34,28% of the sandflies collected in the upper west region were L. longipalpis and the main sources of blood meal were humans, dogs and chickens.

Arte y Astronomía-Astrología de mediados del Quattrocento a Peruzzi y Rafael

Enlazando con el legado de la Antigüedad de Homero a Firmico Materno y el Planisferio Bianchini, se alude a Manilio clave en estas reflexiones sobre Astronomía-Astrología; asimismo quedan referenciadas las aportaciones y variantes medievales orientales y occidentales, hasta Pietro d’Abano en Padua. Tras unas apreciaciones respecto a Venecia como potencia editorial desde fines del siglo XV, y desde luego con referencia a Aldo Manuzio, son considerados varios ejemplos del Quattrocento, de Rimini a Florencia, con vértice en la corte de Ferrara, Borso d’Este, Cosmè Tura y la referencia expresa a Manilio en el Salone dei Mesi. El periplo concluye en Roma, con las contribuciones de Rafael y Peruzzi, en obras para los papas Julio II y León X, así como para el Magnífico Agostino Chigi.

Cuaderno de hule negro

El poeta nicaragüense Rubén Darío fue uno de los principales representantes del modernismo y uno de los escritores que han tenido mayor influencia en la literatura en lengua española de finales del siglo XIX y principios del XX. Su archivo personal, formado por más de 5.000 documentos, reúne materiales producidos entre los años 1893-1923 relativos a su actividad diplomática, a la literaria y a su vida personal. La tipología documental es muy variada: borradores, fotografías, cartas, notas manuscritas, postales, tarjetas de visita, testamentos, telegramas, recortes de prensa, oficios, facturas, menús, etc. Entre todos ellos podemos destacar el "cuaderno de hule negro" que contiene poemas autógrafos de Darío, dibujos infantiles y otros escritos relativos a su ámbito familiar más íntimo. Está inventariado dentro del Archivo con el nº 540, carpeta 6. Se trata de un cuaderno escolar, con tapas de hule, que llevó consigo Rubén Darío durante su viaje a Nicaragua de 1907 a 1908 y que trajo de regreso a España. Tanto las tapas de hule como las páginas acusan la acción del tiempo, así como la huella de otros usos, como el de servir de cuaderno de dibujo para su hijo Rubén Darío Sánchez "Güicho", ya de vuelta en el hogar, apreciándose dibujos infantiles, rayas y garabatos a lápiz en casi todas las páginas, incluso en la que contienen autógrafos de Darío. Faltan cuatro hojas al principio y las páginas han sido numeradas a posteriori. Las primeras 38 contienen una serie de composiciones poéticas autógrafas, completas e incompletas de Rubén, varios poemas editados, algunos de ellos con variantes entre las versiones autógrafas y las versiones impresas, tratándose de los primeros originales, y varios poemas y cinco estrofas de un poema inéditos. Destacan el "Poema de otoño" (páginas 1-11), incompleto, y el manuscrito original del poema "Canción otoñal" (páginas 13-14). Abriendo el cuaderno por el final (páginas 41-59) se observan páginas copiadas por Francisca Sánchez, en letra irregular, probablemente de un capítulo de la novela inédita de Darío "La isla del oro". Francisca fue la compañera del poeta desde 1899 y a ella la enseñaron a escribir el propio Darío y Amado Nervo. En la página 40 del cuaderno se observa una curiosa anotación de puño y letra de Darío con claves telegráficas para comunicarse Rubén y Francisca. Tras el fallecimiento de Rubén en 1916, Francisca Sánchez custodió el conjunto documental en un baúl en su hogar de Navalsaúz (Ávila) hasta 1956, cuando decidió donarlo al Ministerio Español de Educación Nacional. El archivo permaneció en la Facultad de Filología de la Universidad Complutense de Madrid hasta 2008. En esta fecha ingresó en la Biblioteca Histórica de la UCM, institución que alberga y da servicio público del Archivo actualmente

Aproximaciones al análisis del discurso en los estudios identitarios

Este artículo se propone realizar una revisión sobre el estudio de la identidad a partir del análisis del discurso. Dicha propuesta emerge a partir de la investigación desarrollada en mi tesis doctoral en Ciencias Sociales, donde analicé las identificaciones de jóvenes trabajadores precarizados a partir de la teoría de la enunciación. Buscando complementar el recorrido teórico-metodológico allí desarrollado, este artículo busca analizar las potencialidades y limitaciones del análisis del discurso (en sus diversas variantes teóricas) para su aplicación en los estudios identitarios. El mismo se compone de dos ejes de revisión teórica: la identidad como objeto de estudio en las Ciencias Sociales y el análisis del discurso como estrategia metodológica, las cuales serán puestas en diálogo utilizando como insumo las vinculaciones empíricas de ellas realizadas.

Clear hero and dark hero in the cinematographic narration

The role of the hero is changing, slightly irregular, but it’s purpose is constant. The line that separates good from evil is frequently so thin that sometimes the main hero can act in a negative way, casting doubts about his own nature in the pursuit of good. This paper aims to inquire into the character’s heroic condition showing two variations of this archetype in the context of postmodern cinema: the “clear hero”, and the “dark hero”. The former keeps the most traditional values of the hero, while the latter provides a dimension which distinguishes it from the previous one: his experience executing evil as a part of his process to heroici­ty. Postmodernity implies an ambivalence in the character’s identity which can be comparable to the hero’s role, and to his way of making good. The “clear hero” does not imply negative features in the active nature of the hero, whilst the “dark hero” does include them. Through the protagonists of Gladiator (Scott, 2000) and Batman Begins (Nolan, 2005) are offered both heroic perspectives based on a selection of their principal ac­tions which have been subjected to analysis according to some parameters: behavior, implication, and cause. The decisive action will also be signalized, according to the narration’s teleonomy in reason of the favorable or unfavorable sense for the hero. Finally, each character will be observed from its archetype. By analyzing their actions from these perspectives, we observe the clear hero and the dark hero as affordable categories for the heroic role.

Enigmas monetarios en el derecho local. Los mencales y maravedís en los fueros castellanos

La historia de la moneda en la Castilla medieval ha estado siempre mediatizada por la convivencia no siempre armónica entre dos sistemas monetarios muy diferentes. Uno basado en la plata, de origen europeo, otro centrado en el oro, de raíces árabes. La necesidad de conectar y de establecer unas equivalencias entre ellos se convirtió pronto en una necesidad, máxime cuando las monedas de oro incrementaron sus variantes. En esta compleja situación aparecen mencales y maravedís citados conjuntamente en muchos fueros: Zorita, Uclés, Cuenca,... sin que su naturaleza quede del todo clara. Este artículo compara estas referencias y analiza las equivalencias que los unen. Como colofón al trabajo podemos afirmar que los maravedís citados en cada texto corresponden a monedas áureas de distinto peso y valor mientras el mencal es identificado con un ponderal de oro que los relaciona.

The magical transformation contest in the ancient storytelling tradition

El concurso de transformación mágica, esquema narrativo difundido en la tradición popular, se presenta en dos variantes principales: los hechiceros que compiten pueden metamorfosearse en varios seres o crear esos seres por medios mágicos. En cualquier caso el concursante ganador da a luz criaturas más fuertes que superan las de su oponente. La segunda variante fue preferida en el antiguo Cercano Oriente (Sumeria, Egipto, Israel). La primera se puede encontrar en algunos mitos griegos sobre cambiadores de forma (por ejemplo, Zeus y Némesis). El mismo esquema narrativo puede haber influido en un episodio de la Novela de Alejandro (1.36-38), en el que Darío envía regalos simbólicos a Alejandro y los dos monarcas enemigos ofrecen contrastantes explicaciones de ellos. Esta historia griega racionaliza el concurso de cuento de hadas, transfiriendo las fantásticas hazañas de creaciones milagrosas a un plano secundario pero realista de metáfora lingüística.

La fijación léxica en el "Corpus diacrónico del español del Reino de Granada (CORDEREGRA)"

El estudio de las combinaciones léxicas según su grado de fijación y su distinción en combinaciones libres, colocaciones y locuciones ha sido realizado desde la perspectiva sincrónica. Planteamos la posibilidad de aplicar las pautas para distinguir estos tipos de estructuras en materiales de tipo diacrónico. Concretamente, nos basamos en los documentos que componen el Corpus del Español del Reino de Granada (CORDEREGRA) para valorar los materiales de este corpus histórico-lingüístico y comprobar si los criterios sincrónicos se pueden aplicar al estudio de documentos de otros siglos.

La escritura de los jóvenes en los chats en el siglo XXI

El presente ensayo versa sobre la conversación escrita que mantienen los jóvenes hoy en sus chats. Se tratan las diferentes variedades lingüísticas. La escritura de los chats equivale a una conversación: los textos escritos se convierten en textos orales, las conversaciones se transcriben y las normas lingüísticas se rompen. Esto no significa que los jóvenes no sepan cuáles son, pero en los chats, no les interesan.