母鸡输卵管子宫部初级感觉神经元的CB-HRP法定位研究

应用CB-HRP溶液注入母鸡输卵管子宫部浆膜下,四甲基联苯胺(TMB)组织化学呈色,以探察母鸡输卵管子宫部初级感觉神经元的定位。结果表明:母鸡输卵管子宫部的初级感觉神经元位于双侧T1-LS13脊神经节、颈静脉神经节和结状神经节。标记细胞数分布不均,在体左侧多于体右侧,在脊神经节多于颈静脉神经节和结状神经节。在脊神经节内标记细胞有T5-LS1和LS8-LS11前后两个相对集中区,峰值分别在T7和LS11。说明尽管母鸡输卵管子宫部是单侧发育成熟脏器,但其感觉沿双侧脊神经和迷走神经传入中枢,以体左侧传入为主;且

Efecto de concentraciones de aceite de algodón sobre la viabilidad, patogenicidad, estabilidad de la cepa CB-32 de Beauveria bassiana (Bals y vuils)

En Nicaragua, Plutella xylostella es la plaga que causa mayores daños al cultivo de repollo (Brassica oleracea) lo que implica una gran inversión en el control de la misma. Para contribuir a la búsqueda de alternativas para el Manejo integrado de esta plaga se realizó este estudio en las instalaciones del Centro Nacional de Protección Vegetal (CENAPROVE) y en la Universidad Nacional Agraria durante el periodo comprendido entre Febrero 1991 y Febrero 1992. Se evaluó la Cepa CB-32 del hongo entomopatógeno Beauveria bassiana (Bals & Vuils) en 6 formulaciones conteniendo 0%, 2%, 4%, 6%. 8% y 10% de aceite de algodón en agua sobre larvas del segundo instar de P. xylostella. La concentración de conidias en las formulaciones fué de 3.35x108 En un rango de tiempo de 14-30 horas después de la inoculación en PDA se distinguieron con un mayor porcentaje de germinación de conidias las formulaciones con O y 2% de aceite de algodón. Las mismas demostraron mayor sedimentación reportando 0.2 y 0.5 ml seis horas después de ser sometidas a reposo. Para la evaluación del efecto de la radiación solar sobre las conidias de B. bassiana se realizó un análisis de varianza y pruebas de Tukey con un 95% de confianza, las respaldan que las formulaciones pueden separarse en cuatro categorías estadísticas diferentes de las cuales en primer lugar tenemos las formulaciones con O y 2% de aceite que presentan el mayor porcentaje de germinación de conidias expuestas a 24 horas de radiación solar (86.61% y 80.36%) respectivamente. A la variable patogenicidad de las conidias de B. bassiana en huésped de Plutella xylostella se efectúo un análisis de varianza y prueba de Tuckey y resulto que las formulaciones pueden clasificarse en 3 categorías estadísticas, sobresaliendo la patogenicidad de las conidias de las formulaciones con O y 2% de aceite reportando 95 y 80% respectivamente. El tiempo letal para las formulaciones no varió provocando la mortalidad dos días después de la inoculación de las larvas por el método de inmersión.

Estudo da enzima Horseradish peroxidase (HRP) no descoramento dos corantes têxteis Azul Drimaren X-3LR, Azul Drimaren X-BLN, Rubinol Drimaren X-3LR e Azul Drimaren CL-R

O presente trabalho avaliou o potencial da enzima HRP no descoramento dos corantes têxteis: Azul Drimaren X-3LR (DMBLR), Azul Drimaren X-BLN (DMBBLN), Rubinol Drimaren X-3LR (DMR) e Azul Drimaren CL-R (RBBR). Parâmetros como concentração do corante, temperatura, concentração de peróxido de hidrogênio (H2O2) e tempo de reação foram otimizados. Os ensaios de descoramento dos corantes foram realizados a partir desses resultados. As melhores condições reacionais determinadas para os corantes estudados foram: concentração do corante = 120 mg L-1, temperatura = 35C, concentração de H2O2 = 0,55 mM e tempo de reação = 1 hora. Os percentuais de descoramento dos corantes DMBLR, DMBBLN, DMR e RBBR, após o tratamento enzimático foi de 99, 77, 94 e 97%, respectivamente. O tempo reacional de 5 minutos foi suficiente para os corantes DMBLR e RBBR apresentarem elevada porcentagem de descoramento, 96% para ambos. Já os corantes DMBBLN e DMR só apresentaram elevado grau de descoramento após 1 hora de reação, sendo o corante DMBBLN o mais recalcitrante, apresentando uma melhora de 10% na porcentagem de descoramento, após 24 horas de reação. Além do grau de descoramento, também foi avaliada a toxicidade dos corantes antes e após o tratamento enzimático utilizando Daphnia pulex e Artemia salina como bioindicadores de toxicidade. Resultados toxicológicos utilizando Daphnia pulex não foram conclusivos, indicando que esse bioindicador não foi adequado para avaliar a toxicidade dos corantes estudados no meio reacional utilizado. Com o uso da Artemia salina na avaliação toxicológica foi observado uma redução da toxicidade para os corantes DMBLR, DMR e RBBR após tratamento enzimático, e um aumento da toxicidade não significativo para o corante DMBBLN. Os resultados obtidos no trabalho ressaltam a eficiência da enzima HRP no descoramento dos corantes têxteis estudados, sem a geração de produtos tóxicos e prejudiciais ao meio ambiente

神经束路电镜追踪中影响HRP-TMB反应产物因素的分析

采用HRP-TMB-ST法对小鼠延髓下橄榄核下端外侧网状核细胞的HRP-TMB反应产物影响因素分析表明: (1)较高的pH值(7.0以上)是反应产物消失的关键因素; 02mol/L PB, pH5.0-6.0 对HRP-TMB反应灵敏度、反应产物的稳定性及组织超微结构的保存是适宜的; 当pH值相 同时, 缓冲液浓度不同, 反应产物消失的程度和速度也不相同。(2)酒精有稳定产物的作用 。(3)反应前的预浸、灌注液的成分、浓度和固定时间等则与反应产物的丢失无关。此外, 用pH5.5和pH7.0的缓冲液处理的切片经DAB强化、锇化后, 组织的超微结构无明显的差异。 图2表3参18

用CB微核法预测癌细胞的放射敏感性

用细胞质分裂阻断微核技术（简称ＣＢ微核法）研究了癌细胞的放射敏感性，结果表明；ＣＢ 微核法可用来预测癌细胞对重离子和γ射线的放射敏感性，具有省时且成功率高的特点。细胞 受重离子作用后，每双核细胞的微核与剂量的效应关系适合方程 Y＝ c＋ ｋＤｎ或 Y＝ｃ＋ａＤ＋βＤ２，受γ射线作用后，每双核细胞的微核与剂量的效应关系适合方程 Y＝ｃ＋ ａＤ。在相同剂量下，重离子效应高于ｒ射线效应。

A novel hydrogen peroxide sensor based on horseradish peroxidase immobilized in DNA films on a gold electrode

A novel third-generation hydrogen peroxide (H2O2) biosensor was developed by immobilizing horseradish peroxidase (HRP) on a biocompatible gold electrode modified with a well-ordered, self-assembled DNA film. Cysteamine was first self-assembled on a gold electrode to provide an interface for the assembly of DNA molecules. Then DNA was chemisorbed onto the self-assembled monolayers (SAMs) of cysteamine to form a network by controlling DNA concentration. The DNA-network film obtained provided a biocompatible microenvironment for enzyme molecules, greatly amplified the coverage of HRP molecules on the electrode surface, and most importantly could act as a charge carrier which facilitated the electron transfer between HRP and the electrode. Finally, HRP was adsorbed on the DNA-network film. The process of the biosensor construction was followed by atomic force microscopy (AFM). Voltammetric and time-based amperometric techniques were employed to characterize the properties of the biosensor derived. The enzyme electrode achieved 95% of the steady-state current within 2 s and had a 0.5 mu mol l(-1) detection limit of H2O2. Furthermore, the biosensor showed high sensitivity, good reproducibility, and excellent long-term stability.

A hydrogen peroxide biosensor combined HRP doped polypyrrole with ferrocene modified sol-gel derived composite carbon electrode

A novel amperometric biosensor for the detection of hydrogen peroxide is described. The biosensor was constructed by electrodepositing HRP/PPy membrane on the surface of ferrocenecarboxylic acid mediated sol-gel derived composite carbon electrode. The biosensor gives response to hydrogen peroxide in a few seconds with detection limit of 5x10(-7) mol (.) L-1 (based on signal : noise=3). Linear range is up to 0.2 mmol (.) L-1.

辐射效应对LDPE/CB复合物电性能的影响

研究了γ射线辐照交联后的低密度聚乙烯 (LDPE) /碳黑 (CB)复合物在不同温度下的导电性能。应用扫描电子显微镜研究了不同剂量对复合体系正温度系数 (PTC)效应以及负温度系数(NTC)效应的影响。结果表明 ,辐射剂量对PTC和NTC效应的影响很大。在 4 0 0kGy时 ,NTC效应基本消除 ,当辐射剂量继续增加时 ,NTC效应又重新出现。

Designing ionic liquids with boron cluster anions: alkylpyridinium and imidazolium [nido-C(2)B(9)H(11)] and [closo-CB(11)H(12)] carborane salts

A range of new alkylpyridinium and imidazolium carborane salts with [nido-C2B9H12](-), [closo-CB11H12](-), and [RC2B11H11](-) (R = methyl or butyl) anions have been prepared and characterized by physical and thermal methods, including the solid state structures of five of the salts determined by single crystal X-ray diffraction. The tendency of the salts to form low-melting ionic liquids has been assessed; all the salts studied with [nido-C2B9H12](-) anions melted below 100 degrees C and, significantly, have melting points that are 25-85 degrees C lower than those of the corresponding [closo-CB11H12](-) analogs, demonstrating that a wider range of boron-rich ionic liquid materials can be readily accessed.

Cannabidivarin-rich cannabis extracts are anticonvulsant in mouse and rat via a CB 1 receptor-independent mechanism

BACKGROUND AND PURPOSE Epilepsy is the most prevalent neurological disease and is characterized by recurrent seizures. Here, we investigate (i) the anticonvulsant profiles of cannabis-derived botanical drug substances (BDSs) rich in cannabidivarin (CBDV) and containing cannabidiol (CBD) in acute in vivo seizure models and (ii) the binding of CBDV BDSs and their components at cannabinoid CB 1 receptors. EXPERIMENTAL APPROACH The anticonvulsant profiles of two CBDV BDSs (50–422 mg·kg −1 ) were evaluated in three animal models of acute seizure. Purified CBDV and CBD were also evaluated in an isobolographic study to evaluate potential pharmacological interactions. CBDV BDS effects on motor function were also investigated using static beam and grip strength assays. Binding of CBDV BDSs to cannabinoid CB 1 receptors was evaluated using displacement binding assays. KEY RESULTS CBDV BDSs exerted significant anticonvulsant effects in the pentylenetetrazole (≥100 mg·kg −1 ) and audiogenic seizure models (≥87 mg·kg −1 ), and suppressed pilocarpine-induced convulsions (≥100 mg·kg −1 ). The isobolographic study revealed that the anticonvulsant effects of purified CBDV and CBD were linearly additive when co-administered. Some motor effects of CBDV BDSs were observed on static beam performance; no effects on grip strength were found. The Δ 9 -tetrahydrocannabinol and Δ 9 -tetrahydrocannabivarin content of CBDV BDS accounted for its greater affinity for CB 1 cannabinoid receptors than purified CBDV. CONCLUSIONS AND IMPLICATIONS CBDV BDSs exerted significant anticonvulsant effects in three models of seizure that were not mediated by the CB 1 cannabinoid receptor and were of comparable efficacy with purified CBDV. These findings strongly support the further clinical development of CBDV BDSs for the treatment of epilepsy.

Mechanism of charge transport in castor oil-based polyurethane/carbon black composite (PU/CB)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Carbamide Peroxide Determination in Tooth Whitening Using a Reagentless HRP-Biosensor

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Efeito citotóxico do sistema HRP/Indóis em células McCoy in vitro

Pós-graduação em Biociências e Biotecnologia Aplicadas à Farmácia - FCFAR