A novel hydrogen peroxide sensor based on horseradish peroxidase immobilized in DNA films on a gold electrode
Data(s) |
2006
|
---|---|
Resumo |
A novel third-generation hydrogen peroxide (H2O2) biosensor was developed by immobilizing horseradish peroxidase (HRP) on a biocompatible gold electrode modified with a well-ordered, self-assembled DNA film. Cysteamine was first self-assembled on a gold electrode to provide an interface for the assembly of DNA molecules. Then DNA was chemisorbed onto the self-assembled monolayers (SAMs) of cysteamine to form a network by controlling DNA concentration. The DNA-network film obtained provided a biocompatible microenvironment for enzyme molecules, greatly amplified the coverage of HRP molecules on the electrode surface, and most importantly could act as a charge carrier which facilitated the electron transfer between HRP and the electrode. Finally, HRP was adsorbed on the DNA-network film. The process of the biosensor construction was followed by atomic force microscopy (AFM). Voltammetric and time-based amperometric techniques were employed to characterize the properties of the biosensor derived. The enzyme electrode achieved 95% of the steady-state current within 2 s and had a 0.5 mu mol l(-1) detection limit of H2O2. Furthermore, the biosensor showed high sensitivity, good reproducibility, and excellent long-term stability. |
Identificador | |
Idioma(s) |
英语 |
Fonte |
Song YH;Wang L;Ren CB;Zhu GY;Li Z.A novel hydrogen peroxide sensor based on horseradish peroxidase immobilized in DNA films on a gold electrode,SENSORS AND ACTUATORS B-CHEMICAL,2006,114(2):1001-1006 |
Palavras-Chave | #DIRECT ELECTROCHEMISTRY #GRAPHITE ELECTRODE #BIOSENSOR #NANOPARTICLES #MONOLAYERS #PROTEINS #AU(111) #NETWORK |
Tipo |
期刊论文 |