Therapeutic Delivery of miR-200c Enhances Radiosensitivity in Lung Cancer

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Desenvolvimento e validação de uma técnica quantitativa de análise de imagem para avaliação do teste do cometa corado pela prata

The comet assay is a method of DNA damage analysis widely used to quantify oxidative damage, crosslinks of DNA, apoptosis and genotoxicity of chemicals substances as chemical, pharmaceuticals, agrochemicals products, among others. This technique is suitable to detect DNA strand breaks, alkali-labile sites and incomplete excision repair sites and is based on the migration of DNA fragments by microeletroforesis, DNA migrates for the anode forming a “tail”, and the formed image has the appearance of a comet. The slides can be stained with fluorescence or silver, having differences in the microscopy type used for the analysis and the possibility of storage of the slides, moreover, the first one is a stained-method with more difficulties of accomplishment. The image analysis can be performed by a visual way, however, there is a disadvantage as the subjectivity on the results, that can be minimized by an automated method of digital analysis. This process was studied in this report with the aim to perceive the validation of the digital analysis turning it a quantitative method with larger reproductibility, minimizing the variability and imprecision due to the subjective analysis. For this validation we selected 50 comets photographed in a standardized way and printed, afterwards, pictures were submitted to three experienced appraisers, who quantified them manually. Later, the images were processed by free software ImageJ 1.38x, printed and quantified manually by the same appraisers. The intraclass correlation was higher to comet measures after image processing. Following, an algorithm of automated digital analysis from the measures of the comet was developed; the values obtained were compared with those 12 estimated manually after the processing resulting high correlation among the measures. The use of image analysis systems increases ...(Complete abstract click electronic access below)

Avaliação da toxicidade da vinhaça utilizando Oreochromis niloticus (Perciformes: Cichlidae) como organismo teste

Contamination of rivers and lakes, waste disposed by infiltration into the soil as fertilizer, for example, has been highlighted around the world, since such compounds can contain toxic elements, causing changes in aquatic and/or terrestrial ecosystem. Due to the possible presence of these elements in these wastes, it is possible assess damage to organisms exposed through breaks in genetic material and mutations. In this sense, this work aimed at exploring the cytotoxic, mutagenic and genotoxic vinasse pontential, residue used as fertilizer, from the processing of sugar cane into alcohol, using fish (Oreochromis niloticus - Cichlidae) as a test organism. The individuals were exposed to different dilutions of vinasse in the water and later carried out analysis of their erythrocytes using the micronucleus test and nuclear abnormalities associated and the comet assay. In the first bioassay, tilapia exhibited 5% and 10% vinasse concentrations died after 48h. The number of micronucleated erythrocytes was statistically significant for the 1% vinasse concentration and also for nuclear abnormalities such as broken-egg and nuclear bud, when compared with negative and positive controls; were also significant lobed type erythrocytes, when compared to the negative control. The test results of the comet assay were not significant for 1% vinasse when compared to the negative control, due to the high rate of comets with damage control, which is unusual. The same was observed for changes as notched and blebbed type. In the second chemical analyzes conducted, the detection of heavy metals in the vinasse sample, charged only the presence of copper and chromium, the latter with a concentration 89 times higher than in the first test. Differentially the first bioassay, fish exposed to dilution of 5% did not die... (Complete abstract click electronic access below)

Diagnóstico antropogeomorfológico em bacia hidrográfica urbana: o caso do Córrego do Jardim São João, Araras (SP)

Man can transform the nature according to their needs through technique which enables agriculture, cattle raising, constructions of buildings for housing, transport routes, etc. This action has brought significant transformations to the environment, and relief is one of the most changed elements, mainly at urban areas, which concentrates most of the Brazilian population. In these areas geomorphologic systems have undergone alterations in their flow of matter and energy by the action of man. Thus, it is possible o understand the action of man as geomorphologic action, nowadays, the tecnógeno, since the human action can interfere qualitatively and quantitatively in the input and output of these systems. The anthropic geomorphology has contributed to the analyses of the effects due to the urbanization at the form and at the geomorphologic process in the urban watersheds, indicating the transformations at the shape of the relief and the alterations due to the acceleration of the flow of matter and energy. From these questions, this study had the main diagnose the anthropogenic changes of the relief at the basin of Corrego do Jardim São João, Araras (SP), comparing two scenes, 1997 and 2006, through geomorphological mapping evolutionary in the area. The geomorphological feature altered at the basin are directly linked to the use of soil, mainly at the urban use, which caused effects in the relief at the studied area, making possible to define periods of the urban evolution and impacts caused by the urbanization. Among the changes it was found the decrease of the topographic breaks and at the riverbed channels, as the increase of the accumulation of the plain and riverbed terrace. The elaboration of a letter of the legal restrictions of the basin make possible to evaluate some of the environmental urban problems that occur, which are due to the occupation of the relief in the areas of permanent preservation that brings significant impacts to...

Implementação da metodologia TPM em uma indústria do setor de higiene pessoal, saúde e beleza

This work presents a project of Action Search in a consumption industry of the sector of personal care, health and beauty. Following the implementation steps of Autonomous Maintenance Pillar of TPM methodology, stands for Total Productive Maintenance, this work aims to ensure the advancement of some machines in a production line of the company to the next steps of the methodology implementation, demonstrating the results achieved by the TPM. In the company in question, the TPM has been implemented in the past but lost strength over the years and some of its concepts were abandoned, producing then a drop in the equipments efficiency, increased wastes and breaks in the processes, as well as loss in product quality. Then, the need arose to restart the implementation process from the beginning, to strictly follow all the steps of the methodology, ensuring increased efficiency of equipment and processes. Through training and a changing in the company culture, it was possible a joint effort between Operation and Maintenance in order to enhance the knowledge of the operators on their machines. Initially, it was developed a general cleanliness program of equipments so that it could be possible to find the anomalies in the process. Subsequently, operators and maintainers were trained to detect anomalies, enabling equipments to work under their basic conditions of operation and subsequently building provisional standards of equipment cleaning, lubrication and inspection. Through the improvement presented by some indicators such as OEE, wastes, bankruptcies and unavailability, it was proved the importance and the positive effects of TPM implementation in manufacturing

Um estudo de caso sobre a organização escolar e as relações interpessoais dentro do âmbito educacional

Violence and school organization are always current issues. And to investigate these two issues, the present study was carried within a public school in the city for being considered a model of educative institution. The analysis focused on finding out why some institutions are considered good and others not and also to explain how these good relations within the school studied. Therefore, we first observed the school in its stages: entry and exit of students, breaks and classroom activities. Then consult the official documents, such as the Management Plan, Rules, Standards, among others. And finally we interviewed staff, targeting participants in the educational process (principal, teacher, staff, students). Facing this data we verified that a good relationship is needed on the beliefs and the search for a favorable environment is the aim for everyone, especially regarding to teaching-learning processes of a student, that is: teamwork. And as every institution has its flaws, we nee greater participation, especially of what we call school community (parents and relatives).

Avaliação da atenuação da toxicidade de lodo de esgoto por associação com resíduo vegetal da indústria sucro-alcooleira

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

El nuevo regionalismo y el Consejo de Defensa Suramericano

Pós-graduação em Relações Internacionais (UNESP - UNICAMP - PUC-SP) - FFC

Letramento e Oralidade: Notas sobre Pontuação em um Bate-papo Virtual Infantil

Based on a heterogeneous concept of writing, this paper aims at analyzing ‘breaks’ in utterances of a virtual chat for writers between 8 and 12 years of age. The hypothesis is that the segmentation of an utterance in different lines in a short period of time characterizes the writing on the internet as nonconventional and relates it to the prosody and rhythm of the language not restricted to the phonic or graphic dimension of the verbal language.

O uso da aprendizagem baseada em problemas e a atuação docente

Recent national education policy documents have emphasized the importance of methodologies and problem-centered learning processes. The purpose of this article is to promote reflection on the problem-based learning (PBL) understood as a methodology that favors learning processes and the active role of students. PBL emerged in undergraduate medical and has been gaining ground not only in higher education but also in basic education. This conception of teaching-learning breaks with the traditional relationship teacher-student- knowledge, introducing new dynamics of relationships between subjects and those with knowledge. From this perspective the educational performance turns and brings new challenges to the teacher.

Estudo sobre o funcionamento de sistema de controle de tração em veículos automotores

This study explores the subject of traction control systems through bibliografy review on the spot of Mechanical Engineering. This paper has the objetivo of high light the main components and tecnology involved since MaxTrac system in 1971. It describes systems controled by throttle valve and breaks. Additionally, it i also presented basic knowlodged regarding electronic control unit and wheel sensors

Planejamento e controle da produção: reestruturação no layout de apertadeiras eletrônicas em uma linha de produção do ramo de autopeças

In industrial plants, there is a department responsible for planning the use of productive resources to find the best possible way to set out plans in strategic, tactical and operational levels. This department is known as PCP or PPCP, which means Planning and Production Control and Planning, Programming and Production Control, respectively. This work presents the use of some of the tools from this department, in particular the Aggregate Production Planning, to propose a new layout of electronic nutrunners for an auto parts industry output line. Through some process indicators analyzes, was identified some productivity losses. The higher loss rate occurred by the electronic nutrunners breaking, and so this work was focused in these devices. Some premises were adopted for the use of electronic nut runners, setting an ideal cycle time for the operation of the production line and making calculations to define the minimum amount of electronic nutrunners to find the production demand without damaging the equipment. With this work we observed that a relevant factor for the constant breaks of electronic nutrunners is because these are working with overload, in other words, the amount of installed equipment is not enough to supply the demand without failures

Ética e trabalho: Concepção de uma antítese social

Pós-graduação em Serviço Social - FCHS

Análise do binômio saúde: adoecimento de professores e alunos

Pós-graduação em Educação Escolar - FCLAR

Mulheres silenciadas: vivências de viuvez nos anos de chumbo (1970-1980)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)