Vicilina de sementes da leguminosa selvagem Anadenanthera macrocarpa (AmV): purificação, caracterização, efeito deletério e mecanismo de ação para bruquídeo callosobruchus maculatus

Grains and legume seeds are foods that form the basis of the diets of many cultures around the world, winch contritbute to the daily nutrient requirements of humans. Vicilins (7S globulin) are storage proteins found in legume seeds, and may have an additional function constitutive defense of the embryo against pests and pathogens. In this work the vicilin from Anadenanthera macrocarpa - AmV (red-angico), was purified and partially characterized, its effect on development and larval survival and adult emergence of Callosobruchus maculatus was evaluated by determination of LD50, WD50 and ED50 in system bioassay. Purification of vicilin was initiated by the chitin affinity chromatography and then gel filtration (Superdex 75 Tricorn 10x300 mm) FPLC system followed by reverse phase chromatography (C8 phenomenex) on HPLC system. Bioassays WD50 and LD50 for larvae were 0.32% and 0.33% (w:w) respectively, since the ED50 for adults was 0.096%. The probable mechanism of action was evaluated by testing digestibility of AmV in vitro, and observed for the involvement of two fragments vicilins immunoreactive against polyclonal Anti-vicilin from Erythrina velutina (Anti-EvV) about of 22 and 13 kDa chitin binding. The AmV in its native form has been recognized by the anti-EvV, indicating that there is a conserved region in the vicilin and is probably corresponding to the chitin binding domains. These results point to a new vicilin chitin binding that can subsequently be used as a possible biopesticide protein source, in order to control insect pest C. maculatus and confirm literature findings that demonstrate vicilin in the presence of different kinds of ligands to conserved regions chitin not yet characterized

Simulação do escoamento monofásico em um estágio de uma bomba centrífuga utilizando técnicas de fluidodinâmica computacional

Oil production and exploration techniques have evolved in the last decades in order to increase fluid flows and optimize how the required equipment are used. The base functioning of Electric Submersible Pumping (ESP) lift method is the use of an electric downhole motor to move a centrifugal pump and transport the fluids to the surface. The Electric Submersible Pumping is an option that has been gaining ground among the methods of Artificial Lift due to the ability to handle a large flow of liquid in onshore and offshore environments. The performance of a well equipped with ESP systems is intrinsically related to the centrifugal pump operation. It is the pump that has the function to turn the motor power into Head. In this present work, a computer model to analyze the three-dimensional flow in a centrifugal pump used in Electric Submersible Pumping has been developed. Through the commercial program, ANSYS® CFX®, initially using water as fluid flow, the geometry and simulation parameters have been defined in order to obtain an approximation of what occurs inside the channels of the impeller and diffuser pump in terms of flow. Three different geometry conditions were initially tested to determine which is most suitable to solving the problem. After choosing the most appropriate geometry, three mesh conditions were analyzed and the obtained values were compared to the experimental characteristic curve of Head provided by the manufacturer. The results have approached the experimental curve, the simulation time and the model convergence were satisfactory if it is considered that the studied problem involves numerical analysis. After the tests with water, oil was used in the simulations. The results were compared to a methodology used in the petroleum industry to correct viscosity. In general, for models with water and oil, the results with single-phase fluids were coherent with the experimental curves and, through three-dimensional computer models, they are a preliminary evaluation for the analysis of the two-phase flow inside the channels of centrifugal pump used in ESP systems

Térmitas como bioindicadores de qualidade de habitat na caatinga, Brasil: há uma sintonia entre as variáveis estruturais dos habitats e as taxocenoses amostradas?

The composition of termite assemblages was analyzed at three Caatinga sites of the Seridó Ecological Station, located in the municipality of Serra Negra do Norte, in the state of Rio Grande do Norte, Brazil. These sites have been subjected to selective logging, and cleared for pasture and farming. A standardized sampling protocol for termite assemblages (30h/person/site) was conducted between September 2007 and February 2009. At each site we measured environmental variables, such as soil granulometry, pH and organic matter, necromass stock, vegetation height, tree density, stem diameter at ankle height (DAH) and the largest and the smallest crown width. Ten species of termites, belonging to eight genera and three families, were found at the three experimental sites. Four feeding-groups were sampled: wood-feeders, soil-feeders, wood-soil interface feeders and leaf-feeders. The wood-feeders were dominant in number of species and number of encounters at all sites. In general, the sites were not significantly different in relation to the environmental variables measured. The same pattern was observed for termite assemblages, where no significant differences in species richness, relative abundance and taxonomic and functional composition were observed between the three sites. The agreement between the composition of assemblages and environmental variables reinforces the potential of termites as biological indicators of habitat quality

Ecologia alimentar de aves insetívoras de um fragmento de mata decídua do extremo norte da Mata Atlântica

The diet study of birds has contributed historically as a model for use to understanding ecological patterns and strategies used by several other groups of vertebrates, which are observed in season patterns and temporal availability of resources, and other. This study has as objective generate information concerning the diet of insectivorous birds during rainy season and dry season, as well as analyze Index food importance, niche overlap, niche breadth, electivity, and seasonal availability of prey. The study was conducted in a fragment of about 270 ha (center coordinates and 5 º 53'S 35 ° 23'W). The sampling of birds occurred between March 2008 and December 2009 in three pre-established trails. Catches of birds were performed using 10 mist nets placed in line, where each trails was sampled once a month. Samples of pellets were obtained by means of tartar emetic. Sampling of availability of prey occurred between February 2009 to December 2009. We used two methods of sampling (pitfall traps and Shake cloths). We captured 269 individuals of 21 species of insectivorous birds. We collected 4116 invertebrates of which 3259 in the rainy season and 857 in the dry season. We obtained 174 samples stomach, where 10 species were exclusively insectivorous diet, nine fed on insect/plant material, an insect/plant material/vertebrate and one for insect/vertebrate. During the rainy season was observing difference between the consumption of items with higher food importance. The Coleoptera was item with higher food importance (73%), followed by Formicidae (7%) and Araneae (6%). During the dry season, no difference was found difference between the consumption of items with higher food importance. The Coleoptera was item with higher food importance (34%), followed by seeds (29%) and Formicidae (18%). The highest levels of niche overlap occurred during the rainy season, while the dry season was characterized by high levels of niche 11 segregation. This indicates that the local insectivorous birds community was structured differently between periods. No was found correlation between the values of niche breadth to the mean weight of the body size. We observed seasonal patterns in prey availability, with the peak availability of invertebrates observed seasonal patterns in rainy season. The insectivorous birds selected the same species richness during both periods, showing a specialized diet. Thamnophilus pelzelni was the only species that had their diet influenced by seasonality. Regarding the overall diet of insectivorous birds, observed a high consumption of prey, whose food availability caused the birds could invest and increase their food resources

Mortalidade da população em idade ativa (PIA) por doença cardiovascular e câncer nas capitais e nas regiões metropolitanas brasileiras: associação com indicadores socioeconômicos

Universidade Estadual do Rio Grande do Norte

Prática desportiva e qualidade de vida de crianças do bairro de nova descoberta Natal / RN

Objective: This study aimed to investigate the contribution of sports activities for children participating in social programs. Method: It s dealt with in a survey of a descriptive nature. The sample was composed of 51 participants from the Project Nova Descoberta, ranging in ages from 8 to 17, males (n=29) and females (n=22). A semi-directed interview was applied with 10 items over a period of 2 consecutive years (2007-2008) with children and adolescents, besides obtaining documental data in the schools that have an agreement with the Project. Results: The performance of the participants in the groups that joined and did not join in terms of schoolwork production in the year 2007 did not present statistical differences (p<0,05). A significant difference (p<0,05) was observed in the discipline of Portuguese when compared between the groups, with a higher average for the students who joined in 2008. Discussion: In the face of the context found in social sports projects, emerges a series of indicators that contribute for a reliable evaluation. The manifestation of other characteristics in the environment could be interfering in the participation and involvement in physical activity and consequently in the health and quality of life situation of the children and adolescents. Conclusion: It was confirmed that the school work performance indicators can be taken together with other indicators, such as a development of various abilities, participation in other activities, motivation for the activities, behavior and attitudes at home and at school, for evaluation of social projects

Fatores associados à mortalidade dos idosos com as condições de vida da população do Nordeste do Brasil

As condições de vida são usualmente entendidas como importantes fatores intervenientes das causas básicas de mortalidade e da qualidade de vida da população. Ao considerar esta questão em uma população idosa e sua influência nas causas mais relevantes que as levam à morte, teve-se como objetivo principal, estudar a associação da mortalidade dos idosos por doenças cardiovasculares para ambos os sexos, com indicadores que expressam as condições de vida da população do Nordeste, nos anos 2000 e 2010. Para atingir tal propósito foi traçado um desenho de estudo ecológico com cortes transversais nos anos 2000 e 2010 tendo como unidades de análises as microrregiões e a mesorregiões do Nordeste. Utilizou-se a Modelagem de Equações Estruturais (MEE) para explicar essas associações, tomando como base as estatísticas de óbitos por doenças cardiovasculares (construto endógeno) e variáveis socioeconômicas e demográficas (construto exógeno) das microrregiões do Nordeste, para o ano 2000. Utilizou-se o Método dos Componentes Principais como recurso metodológico na construção de um índice sintético que permitiu classificar as condições de vida nas mesorregiões do Nordeste, tomadas como unidades de análises, nos anos 2000 e 2010, de acordo com as condições de vida e as principais causas de mortalidade por doenças cardiovasculares. A aplicação da MEE sugeriu uma forte associação do construto exógeno condições de vida, composto pelos indicadores: anos de estudo dos homens idosos, percentual de idosos em domicílios com banheiro/água encanada e probabilidade de sobrevivência aos 60 anos, com a variável desfecho taxa de mortalidade por doenças cardiovasculares dos homens e mulheres idosos. O índice sintético construído permitiu identificar os diferentes ritmos de envelhecimento populacional entre as regiões e distintos processos de evolução dos níveis de mortalidade pelas doenças cardiovasculares, muitas delas compatíveis com as condições de vida no Nordeste. Os resultados sugerem que o construto condições de vida está fortemente associado por esta causa de morte entre os idosos para ambos os sexos no Nordeste do Brasil. Neste sentido, sugere-se que estes resultados sejam considerados como relevantes para formulação de políticas de saúde regionais voltadas para este contingente populacional do Nordeste do Brasil

Farmacêuticos e suas atividades em farmácias comunitárias: uma análise de perfil

The aim of this study was to establish the profile of the pharmacist technician responsible for community pharmacies in the city of Natal/RN, featuring personal elements, perceived their role and place of pharmaceutical care, levels of job satisfaction, type and quality of services provided in human and structural framework. To that end, we made an exploratory cross-sectional study applying a questionnaire containing open and closed questions, which was applied to pharmaceutical technicians responsible for community pharmacies in Natal/RN, from September 2010 to September 2011. The sample was established by calculating the simple random sample, with a confidence level of 95% and a significance level of 0.05. To evaluate the satisfaction level of the activities performed by pharmacists in community pharmacies was used Simple Satisfaction Scale (Likert, 1935). To assess the attitudes and perceptions of pharmacists in relation to aspects of pharmaceutical care, we used the Model Attitude toward the object (Fishbein, Ajzen, 1975). The answers were converted into data were analyzed statistically using Epi Info 3.5.2 The results showed that the strengths and weaknesses in relation to the profile of the pharmacist and their activities in community pharmacies in Natal/RN are not different in other cities in the country . The most important aspects were: 51% (n = 90) of the establishments visited, the pharmacist was absent; 46% (n = 80) did not have postgraduate and of those who are or have completed 33% (n = 51) are in the area of Clinical Analysis; 56% (n = 98) 08h for day work and 64% (n = 111) claim that this load influence its performance; 83% (n = 146) receive as salary, the floor pharmacist regarding the state of Rio Grande do Norte; 44% (n = 76) are unhappy about the salary, which is the main difficulty cited; 78% (n = 136) say they are always sought by users and the receptivity of these considered good (52%, n = 91). The activities of higher satisfaction are those related to pharmaceutical care and lower the administrative. As regards attitudes and perceptions, the score was more negative to the question 'if the pharmacist feels working as a team with the doctor', in which 59% (n = 103) responded 'never'. 49% (n = 86) reported being "able" to take questions from users and 39% (n = 68) are 'dissatisfied' with respect to the structure of the practice of pharmacy to pharmaceutical care. Action is needed on the obstacles to the exercise of the pharmacist in the solution and minimize the negative and positive stimulus to

UMA FONTE DE ÁGUA VIVA: A participação das mulheres no assentamento Arizona São Miguel do Gostoso-RN

Cette étude cherche á comprendre la signification sociale de La participation des féminine dans le contexte de l organisation des périmétres de réforme agraire. Elle est construite autour de la mise en évidence des effets de cette participation dans la vie des femmes et dans les relations qu elles établissent avec leurs familles et avec la communauté du périmétre. Les données ont été obtenues á partir d observations participatives, entretiens et discussions. Elles ont permis d identifier les relations de genre et de pouvoir, construites autour du processus d organisation du périmétre de Arizona, dans la préfecture de São Miguel do Gostoso, Rio Grande do Norte, région de l étude. L analyse s est concentrée sur lês dynamiques liées á la lutte pour l accés á la terre, pour identifier différentes formes de participation de femmes et d hommes, en recherche d alternatives pour s installer dans ce contexte. On constate la participation des femmes á diverses formes d activités inhérentes á l organisation du quotidien en milieu rural, principalement pour la recherche d améliorations des conditions de vies, pour elles et leurs familles. Pour cela elles tissent des liens de solidarité et d amitié, occupent les espaces politiques et de décision, dans le périmétre de réforme agraire et de façon plus large, au niveau de la préfecture. Elles pratiquent le travail en groupe, comme forme d organisation et développent des activités productives dans le domaine de l agroécologie. On constate que les femmes ont une place essentielle dans l économie domestique et s affirment comme des acteurs productifs et sociaux. Il faut noter enfin, que cette étude est une invitation au débat, sur le quotidien de l acteur féminin dans les périmétres de réforme agraire, dans la mesure oú le substrat symbolique est avancé comme un support pour expliquer la participation différenciée de la femme dans la lutte pour la terre et dans son quotidien

A dimensão simbólica e midiática da política e a história da dona de casa que virou guerreira : a construção da imagem pública de Wilma de Faria em 2002 e 2006

This paper examines two aspects. First, the symbolic dimension of politics and some of the elements that make up this universe, as the scenario, the representation, the myth, the spectacle, the media and the political and electoral marketing. We assume that the policy brings together a set of traits related to both reason and the human subjectivity, and can not be summed up in just a few calculations based on rationality. In the case of elections, in a process (ritual, according Irlys Barrier) of choice, there is a meeting of two systems of representations: to that transmitted by a political actor, in a scene from a particular context, based on a life trajectory unique, and the other from the public, crossed by social relations, situations own wishes, desires, expectations and unique perspectives. Between them there are the means of mass media (especially television), and with them the advent of language media and advertising applied to politics, changing the layout of public visibility and inaugurating what Rejane Accioly Carvalho will call the "aesthetics of mostrabilidade". This does not necessarily mean a preponderance of media on politics as a whole but only its adaptation to that with regard to contact with the public, the ad extra portion of the policy, according to Wilson Gomes. In a second aspect, try to apply these elements to a specific study to verify them in building an effective public image, in this case, the current governor of Rio Grande do Norte, Wilma de Faria. The concept of public image is from the book of Wilson Gomes The transformation was visible in the mass media, and relates to a conceptual image to fix "personality traits" through political history, personal conduct, action of image makers and the public reception. For this we will review some videos aired on Free Time for political propaganda in the years 2002 and 2006.

O SABOR DO SANGUE: uma análise sociocultural do chouriço sertanejo

Cette étude propose de réaliser une lecture du chouriço, une confiture faite avec du sang, fabriquée et consommée dans tout le Seridó potiguar. Grace à l observation ethnographique réalisée entre 2003 et 2007, nous découvrons une économie domestique, une organisation sociale, des formes de sociabilité et de solidarité contruites autour de l élevage des porcs et des pratiques culinaires. Cette observation a aussi révélé un style alimentaire, des discours, des perceptions, des goûts et des comportements relatifs à la consommation de la confiture qui résultent, em grande partie, de la relation dynamique entre une norme idéalisée et des pratiques observées qui ont montré des tensions et des contradictions entre ce qui est dit et ce qui est fait. Avec l étude du chouriço, nous reálisons une lecture de la culture alimentaire; notre intention a été de problématiser l applicabilité des normes sociales et leur inscription dans la réalité. Ainsi, em faisant une analyse simbolique du chouriço, nous considérons ensemble les aspects sociaux et symboliques et nous décrivons comment les habitants du Seridó pensent leur alimentation en corrélation avec les pratiques alimentaires et les formes de sociabilité. L étude a révélé que les príncipes de confiance et d interconnaissance, fondés sur les liens sociaux, structurent les relations constituées autour de l élevage des porcs et de la production, de la distribution et de la consommation du chouriço. D un point de vue symbolique, le sang est central dans le style alimentaire: il apparaît comme une anti-nourriture et révèle des prohibitions et des transgressions. La contrastivité entre les pratiques et les discours est en relation directe avec la nature du chouriço, fait avec le sang du porc et considéré comme un dessert

Caminho possível para o desenvolvimento do RN: a utopia do Polo Gás Sal

La présente étude sur la modernisation économique qui s est opérée dans le Rio Grande do Norte, constitue un débat concernant les tentatives de faire aboutir un projet de développement industriel fondé sur l exploitation des matières premières locales telles que le sel, le calcaire, les eaux mères et le pétrole, entre les années 70 et 90 du XXe siècle. Elle cherche à montrer, à partir de l observation de l action planifiée de l État national et régional, soutenu par le capital international et par les groupes économiques et politiques locaux, comment on a tenté d implanter, dans des conjonctures économiques distinctes, une industrie d intrants de base moyennant la création de deux pôles de développement : le Pólo Químico Industrial (1974) et le Pólo Gás Sal (1996). Elle est centrée sur l analyse des actions orientées vers la concrétisation de ces pôles, et prendra pour objet les politiques de développement émanant de l État national, en particulier celles qui relevaient du II PND et qui visaient, au cours des années 70, à l implantation d une industrie décentralisée dans la région Nordeste ; les règles fixées par le Plano Nacional de Desestatizações, destinées à promouvoir les privatisations des entreprises publiques brésiliennes, lesquelles ont rendu possible la vente d Alcanorte au groupe industriel commandé par Fragoso Pires, et les politiques définies par l État national et régional, rassemblées sous le titre de « guerre fiscale » afin d attirer des capitaux internationaux susceptibles de rendre viable le Pólo Gás Sal. Il y est démontré que l État a coordonné un ensemble d actions dans les domaines de l infrastructure et des exonérations fiscales et financières, dans le but d accélérer la transition d une économie primaire et exportatrice vers une économie moderne, industrialisée. Et que le concours simultané de forces économiques et sociales particulièrement expressives le capital national et international, les élites locales n a pas suffi pour promouvoir la modernisation industrielle escomptée des secteurs chimique et pétrochimique. Parmi les différentes raisons qui ont contribué à un tel échec, on peut mentionner : l absence d une accumulation interne préalable de capital ; la dépendance de l économie locale par rapport à la technologie et au capital des groupes économiques internationaux ; l inconsistance des stratégies de développement relevant de la politique qui caractérisait la « guerre fiscale », et la fragilité de la représentation politique locale

Antonin artaud: a crueldade pelos desenhos e autorretratos

Antonin Artaud, a name that reminds us of areas ranging from theater to poetry, linguistics to psychoanalysis, is a multipurpose name that transits as poet, painter, writer, actor, screenwriter, playwright and theater director. Artaud s route is raw material for researchers of various hues interested in a life and work that allows panning in different fields of knowledge. It raises the question of language and manipulation of signals in terms of magical forces and the relationship maintained through them with the cosmos and the divine. Artaud searches through a language of signals, gestures and objects that express themselves by objective forms and the use of words as solid objects. For him, the language of words must give way to the language of signals, whose objective aspect is what strikes us most immediately. Our work indicates the possibility of realization and recognition of the aesthetic of cruelty present in the writing drawings of artaudian s work, realizing thus, that art as a record of culture hence as double of life allows us a more critical and transforming look to the society, thinking about the aesthetics of cruelty as Artaud proposes and thinks cruelty: as appetite for life. Our dialogue held during the construction of this journey has the company, besides the one of Antonin Artaud, other authors such as Jacques Derrida, Gilles Deleuze and Felix Guatarri, among others which, during the hike and framing of this route help us to think about the aesthetics of cruelty in an Artaudian perspective

Mudanças demográficas e homicídios. Que relação é essa? Um estudo nas Regiões Metropolitanas de Maceió, Natal, Recife e São Paulo

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior

Mortalidade infantil e condições socioeconômicas nas microrregiões do Nordeste Brasileiro

The study aims to answer the following question: what are the different profiles of infant mortality, according to demographic, socioeconomic, infrastructure and health care, for the micro-regions at the Northeast of Brazil? Thus, the main objective is to analyze the profiles or typologies associated mortality levels sociodemographic conditions of the micro-regions, in the year 2010. To this end, the databases of birth and death certificates of SIM and SINASC (DATASUS/MS), were taken from the 2010 population Census microdata and from SIDRA/IBGE. As a methodology, a weighted multiple linear regression model was used in the analysis in order to find the most significant variables in the explanation child mortality for the year 2010. Also a cluster analysis was performed, seeking evidence, initially, of homogeneous groups of micro-regions, from of the significant variables. The logit of the infant mortality rate was used as dependent variable, while variables such as demographic, socioeconomic, infrastructure and health care in the micro-regions were taken as the independent variables of the model. The Bayesian estimation technique was applied to the database of births and deaths, due to the inconvenient fact of underreporting and random fluctuations of small quantities in small areas. The techniques of Spatial Statistics were used to determine the spatial behavior of the distribution of rates from thematic maps. In conclusion, we used the method GoM (Grade of Membership), to find typologies of mortality, associated with the selected variables by micro-regions, in order to respond the main question of the study. The results points out to the formation of three profiles: Profile 1, high infant mortality and unfavorable social conditions; Profile 2, low infant mortality, with a median social conditions of life; and Profile 3, median and high infant mortality social conditions. With this classification, it was found that, out of 188 micro-regions, 20 (10%) fits the extreme profile 1, 59 (31.4%) was characterized in the extreme profile 2, 34 (18.1%) was characterized in the extreme profile 3 and only 9 (4.8%) was classified as amorphous profile. The other micro-regions framed up in the profiles mixed. Such profiles suggest the need for different interventions in terms of public policies aimed to reducing child mortality in the region