998 resultados para Flavobacterium columnare G4


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Starch is found in sugarcane as a storage polysaccharide. Starch concentrations vary widely depending on the country, variety, developmental stage, and growth conditions. The purpose of this study was to determine the starch content in different varieties of sugarcane, between May and November 2007, and some characteristics of sugarcane starch such as structure and granules size; gelatinization temperature; starch solution filterability; and susceptibility to glucoamylase, pullulanase, and commercial bacterial and fungal α-amylase enzymes. Susceptibility to debranching amylolytic isoamylase enzyme from Flavobacterium sp. was also tested. Sugarcane starch had spherical shape with a diameter of 1-3 µm. Sugarcane starch formed complexes with iodine, which showed greater absorption in the range of 540 to 620 nm. Sugarcane starch showed higher susceptibility to glucoamylase compared to that of waxy maize, cassava, and potato starch. Sugarcane starch also showed susceptibility to debranching amylolytic pullulanases similar to that of waxy rice starch. It also showed susceptibility to α-amylase from Bacillus subtilis, Bacillus licheniformis, and Aspergillus oryzae similar to that of the other tested starches producing glucose, maltose, maltotriose, maltotetraose, maltopentose and limit α- dextrin.

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The odor and taste profile of cocoa bean samples obtained from trees cultivated in southern Mexico were evaluated by trained panelists. Seven representative samples (groups) of a total of 45 were analyzed. Four attributes of taste (sweetness, bitterness, acidity and astringency), and nine of odor (chocolate, nutty, hazelnut, sweet, acidity, roasted, spicy, musty and off-odor) were evaluated. A sample (G7) with higher scores in sweet taste and sweet and nutty odors was detected, as well as a high association between these descriptors and the sample, analyzed through principal component analysis (PCA). Similarly, samples that showed high scores for non-desired odors in cocoas such as off-odor and musty were identified and related by PCA to roasted odor and astringent taste (G2 and G4). Based on this scores, the samples were listed in descending order by their sensory quality as G7> G5> G6> G3> G1> G4> G2.

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Tämän tutkimuksen lähtökohtana oli havainto siitä, että maailmanlaajuisen jihadismin toimet muistuttivat suuresti kumouksellista sodankäyntiä. Tarkastelemalla ilmiötä lähemmin oli nopeasti havaittavissa, että globaaliksi jihadistiseksi liikkeeksi tunnistettava toimija kävi ja oli kehittänyt taitoa käydä tällaista sotaa. Näin keskeiseksi tavoitteeksi tutkimukselle muodostuikin selvittää miten globaali jihadistinen liike oli kehittänyt kumouksellisen sodankäynnin sotataitoa ja sotataidollista ajattelua. Tämän tutkimuksen asetelma on perusluonteeltaan vertaileva. Se tarkastelee teoriaohjaavan sisällönanalyysin kautta kumouksellista sotataidollista kehitystä, tarkemmin sotataidollisen ajattelun kehitystä, vertaamalla globaalin jihadistisen liikkeen strategista ajattelua kumouksellista sotaa käyneiden strategien klassisiin mallinnuksiin. Strategioiden välinen vertailu suoritettiin tarkastelemalla globaalin jihadistisen liikkeen strategista ajattelua klassisista strategioista löydettävien vertailutasojen kautta. Globaalia jihadismia tarkastellessa kävi selville sen monisyinen luonne. Tätä toimijaa ei sellaisenaan voi kutsua yhdeksi organisaatioksi, eikä pelkästään ideologiaksi. Globaali jihadistinen liike tässä tutkimuksessa hahmotettiin sosiaaliseksi liikkeeksi, joka pitää sisällään yhteisen päämäärän eteen pyrkiviä, samaa ideologiaa tunnustavia organisaatiota, soluja ja yksittäisiä toimijoita. Vertailevan analyysin perusteella voidaan sanoa, että sosiaalisliikemäisestä luonteesta huolimatta ja osaltaan sen ansiosta globaali jihadistinen liike on kyennyt kehittämään kumouksellisen sodan strategista ajattelua suuntaan, jossa hajaantunut organisaatiomuoto, rajaamaton toiminta-alue ja kauas tulevaisuuteen tähtäävä tavoitteenasettelu ovat yhteiseen ideologiseen katsontaan sitoutuneina muovanneet liikkeestä toimijan, joka on kyennyt selviämään hengissä mittavista vastatoimista huolimatta. Se on myös kyennyt ylläpitämään itsetarkoitukseksi muodostunutta operaatiotahtia tavalla joka asettaa modernille turvallisuusympäristölle jatkuvia ja vakavia haasteita.

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The addition of commercial nitrifying bacterial products has resulted in significant improvement of nitrification efficiency in recirculating aquaculture systems (RAS). We developed two nitrifying bacterial consortia (NBC) from marine and brackish water as start up cultures for immobilizing commercialized nitrifying bioreactors for RAS. In the present study, the community compositions of the NBC were analyzed by universal 16S rRNA gene and bacterial amoA gene sequencing and fluorescence in situ hybridization (FISH). This study demonstrated that both the consortia involved autotrophic nitrifiers, denitrifiers as well as heterotrophs. Abundant taxa of the brackish water heterotrophic bacterial isolates were Paenibacillus and Beijerinckia spp. whereas in the marine consortia they were Flavobacterium, Cytophaga and Gramella species. The bacterial amoA clones were clustered together with high similarity to Nitrosomonas sp. and uncultured beta Proteobacteria. FISH analysis detected ammonia oxidizers belonging to b subclass of proteobacteria and Nitrosospira sp. in both the consortia, and Nitrosococcus mobilis lineage only in the brackish water consortium and the halophilic Nitrosomonas sp. only in the marine consortium. However, nitrite oxidizers, Nitrobacter sp. and phylum Nitrospira were detected in both the consortia. The metabolites from nitrifiers might have been used by heterotrophs as carbon and energy sources making the consortia a stable biofilm.

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The composition and variability of heterotrophic bacteria along the shelf sediments of south west coast of India and its relationship with the sediment biogeochemistry was investigated. The bacterial abundance ranged from 1.12 x 103 – 1.88 x 106 CFU g-1 dry wt. of sediment. The population showed significant positive correlation with silt (r = 0.529, p< 0.05), organic carbon (OC) (r = 0.679, p< 0.05), total nitrogen (TN) (r = 0.638, p< 0.05), total protein (TPRT) (r = 0.615, p< 0.05) and total carbohydrate (TCHO) (r = 0.675, p< 0.05) and significant negative correlation with sand (r = -0.488, p< 0.05). Community was mainly composed of Bacillus, Alteromonas, Vibrio, Coryneforms, Micrococcus, Planococcus, Staphylococcus, Moraxella, Alcaligenes, Enterobacteriaceae, Pseudomonas, Acinetobacter, Flavobacterium and Aeromonas. BIOENV analysis explained the best possible environmental parameters i.e., carbohydrate, total nitrogen, temperature, pH and sand at 50m depth and organic matter, BPC, protein, lipid and temperature at 200m depth controlling the distribution pattern of heterotrophic bacterial population in shelf sediments. The Principal Component Analysis (PCA) of the environmental variables showed that the first and second principal component accounted for 65% and 30.6% of the data variance respectively. Canonical Correspondence Analysis (CCA) revealed a strong correspondence between bacterial distribution and environmental variables in the study area. Moreover, non-metric MDS (Multidimensional Scaling) analysis demarcated the northern and southern latitudes of the study area based on the bioavailable organic matter

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Partiendo de la importancia que ha adquirido la Responsabilidad Social (RS) a nivel mundial y bajo el entendimiento que la cadena de suministro es de importancia para la efectiva ejecución de una política de RS de cualquier organización, en el presente trabajo se pretende realizar un diagnóstico de la RS de los contratistas que tienen potenciales impactos en los asuntos considerados pertinentes por Equión Energía según los lineamientos de la norma ISO 26000. El proceso se centra en Equión Energía, organización petrolera radicada en Colombia con operación de proyectos de exploración y explotación de hidrocarburos en el departamento de Casanare. Para el desarrollo de las actividades la empresa, Equión cuenta con aproximadamente 150 organizaciones contratistas a nivel internacional y nacional. Mediante la realización del diagnóstico a una muestra representativa del grupo de contratistas, el estudio servirá como insumo para definir una ruta de ajuste gradual para promover la RS en la cadena de suministro de Equión. El fin último del ajuste es mejorar la gestión de la RS en los procesos de compras y contratación, contribuyendo así, con el cumplimiento de la misión y visión de la compañía donde se espera que la organización contribuya con el desarrollo sostenible de la región donde opera y del país.

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El objetivo de este artículo es describir la operación del área de Sostenibilidad de Alpina S.A. en Colombia, como parte de un proyecto de investigación que permita entender el funcionamiento de dicha área. Los métodos de recolección de información utilizados fueron la entrevista semiestructurada y la consulta de fuentes secundarias. Para lograr el objetivo, se definieron indicadores agrupados en cuatro variables así: Evolución del área, Plataforma estratégica, División del trabajo y Coordinación del trabajo. La información recolectada se codificó y clasificó de acuerdo a las variables de análisis. Los resultados exponen que el área de Sostenibilidad actúa de manera transversal en la organización y está integrada a la estrategia del negocio, siendo parte fundamental en las decisiones y los proyectos de las distintas áreas de la compañía.

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Averiguar el valor de los modelos de desamparo aprendido y la teoría de la atribución en su papel explicativo. Ver si la incontrolabilidad produce desamparo. Ver si la atribución causal mediatiza la aparición de síntomas. Medir el efecto de aprendizaje con elementos comunes y del cuestionario atribucional. Muestreo por selección al azar. Número: 123 estudiantes de BUP de un colegio privado. Generalización: estudiantes de BUP. Los sujetos fueron asignados al azar a uno de los 5 grupos siguientes: G1: prueba inicial resoluble, cuestionario y prueba de anagramas. G2: prueba inicial irresoluble, cuestionario y prueba de anagramas. G3: prueba inicial resoluble y prueba de anagramas. G4: prueba inicial irresoluble y prueba de anagramas. G5: prueba de anagramas. Variable independiente: prueba de incontrolabilidad. VD: cuestionario atribucional. VD: prueba de anagramas. Prueba de incontrolabilidad: juego de cartulinas de colores, produce la situación de control y no control. Cuestionario atribucional: medir el estilo atribucional de los sujetos con respecto al éxito o al fracaso en la tarea previa. Prueba de anagramas: juego de cartulinas con letras, mide el número de aciertos y de errores, las latencias post error, latencia post acierto y latencia total. Contraste de diferencias de medias. La conclusión más notable a que se llega después de ver los resultados arrojados por el tercer estudio, es que los factores práctica y cuestionario son ciertamente importantes, si no determinantes, en relación con dichos resultados. Los datos indican que ambos contribuyen a mejorar la ejecución de la tarea de prueba y que dicha contribución parece ser distinta en condiciones de resolubilidad o irresolubilidad. Asimismo, podemos suponer que ambos efectos interactúan, no simplemente se suman, de alguna forma compleja, que deberá ser investigada con mayor detalle. En estos estudios se aporta más evidencia sobre la importancia de evaluar los componentes motivacionales, pues se supone que este componente contrarresta al efecto de incontrolabilidad, de forma que los sujetos sometidos a la no contingencia rinden más por un incremento de la motivación en dirección opuesta a la del desamparo, derivado, quizás, de haber realizado la primera tarea y de haber contestado al cuestionario. Por lo tanto, en el futuro será necesario tener presente el efecto motivacional a la hora de planificar estudios de este tipo.

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[1] High-elevation forests represent a large fraction of potential carbon uptake in North America, but this uptake is not well constrained by observations. Additionally, forests in the Rocky Mountains have recently been severely damaged by drought, fire, and insect outbreaks, which have been quantified at local scales but not assessed in terms of carbon uptake at regional scales. The Airborne Carbon in the Mountains Experiment was carried out in 2007 partly to assess carbon uptake in western U.S. mountain ecosystems. The magnitude and seasonal change of carbon uptake were quantified by (1) paired upwind-downwind airborne CO2 observations applied in a boundary layer budget, (2) a spatially explicit ecosystem model constrained using remote sensing and flux tower observations, and (3) a downscaled global tracer transport inversion. Top-down approaches had mean carbon uptake equivalent to flux tower observations at a subalpine forest, while the ecosystem model showed less. The techniques disagreed on temporal evolution. Regional carbon uptake was greatest in the early summer immediately following snowmelt and tended to lessen as the region experienced dry summer conditions. This reduction was more pronounced in the airborne budget and inversion than in flux tower or upscaling, possibly related to lower snow water availability in forests sampled by the aircraft, which were lower in elevation than the tower site. Changes in vegetative greenness associated with insect outbreaks were detected using satellite reflectance observations, but impacts on regional carbon cycling were unclear, highlighting the need to better quantify this emerging disturbance effect on montane forest carbon cycling.

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The polymorphisms of endothelial nitric oxide synthase (eNOS) are associated with reduced eNOS activity. Aerobic exercise training (AEX) may influence resting nitric oxide (NO) production, oxidative stress and blood pressure. The purpose of this study was to investigate the effect of AEX on the relationship among blood pressure, eNOS gene polymorphism and oxidative stress in pre-hypertensive older people. 118 pre-hypertensive subjects (59 +/- A 6 years) had blood samples collected after a 12 h overnight fast for assessing plasma NO metabolites (NOx) assays, thiobarbituric acid reactive substances (T-BARS) and superoxide dismutase activity (ecSOD). eNOS polymorphism (T-786C and G-894T) was done by standard PCR methods. All people were divided according to the genotype results (G1: TT/GG, G2: TT/GT + TT, G3: TC + CC/GG, G4: TC + CC/GT + TT). All parameters were measured before and after 6 months of AEX (70% of VO(2 max)). At baseline, no difference was found in systolic and diastolic blood pressure, ecSOD and T-BARS activity. Plasma NOx levels were significantly different between G1 (19 +/- A 1 mu M) and G4 (14.2 +/- A 0.6 mu M) and between G2 (20.1 +/- A 1.7 mu M) and G4 (14.2 +/- A 0.6 mu M). Therefore, reduced NOx concentration in G4 group occurred only when the polymorphisms were associated, suggesting that these results are more related to genetic factors than NO-scavenging effect. After AEX, the G4 increased NOx values (17.2 +/- A 1.2 mu M) and decreased blood pressure. G1, G3 and G4 decreased T-BARS levels. These results suggest the AEX can modulate the NOx concentration, eNOS activity and the relationship among eNOS gene polymorphism, oxidative stress and blood pressure especially in C (T-786C) and T (G-894T) allele carriers.

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A funerary gold mask from the Museum of Sican, Ferranafe, Peru was analyzed in 30 different areas using a portable equipment using energy-dispersive X-ray fluorescence. It was deduced from the measurements that the main sheet of the mask and the majority of the pendants have a similar composition and are made of tumbaga, which means a poor gold alloy enriched at the surface by depletion gilding, and have a similar `equivalent` gilding thickness of about 5 mu m. The nose, also on tumbaga, has different composition and a thickness of about 8 mu m. The clamps are on gilded or on silvered copper. The red pigment dispersed on the surface of the mask is cinnabar. Copyright (C) 2009 John Wiley & Sons, Ltd.

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The purpose of this study was to evaluate the influence of different light sources and photo-activation methods on degree of conversion (DC%) and polymerization shrinkage (PS) of a nanocomposite resin (Filtek (TM) Supreme XT, 3M/ESPE). Two light-curing units (LCUs), one halogen-lamp (QTH) and one light-emitting-diode (LED), and two different photo-activation methods (continuous and gradual) were investigated in this study. The specimens were divided in four groups: group 1-power density (PD) of 570 mW/cm(2) for 20 s (QTH); group 2-PD 0 at 570 mW/cm(2) for 10 s + 10 s at 570 mW/cm(2) (QTH); group 3-PD 860 mW/cm(2) for 20 s (LED), and group 4-PD 125 mW/cm(2) for 10 s + 10 s at 860 mW/cm(2) (LED). A testing machine EMIC with rectangular steel bases (6 x 1 x 2 mm) was used to record the polymerization shrinkage forces (MPa) for a period that started with the photo-activation and ended after two minutes of measurement. For each group, ten repetitions (n = 40) were performed. For DC% measurements, five specimens (n = 20) for each group were made in a metallic mold (2 mm thickness and 4 mm diameter, ISO 4049) and them pulverized, pressed with bromide potassium (KBr) and analyzed with FT-IR spectroscopy. The data of PS were analyzed by Analysis of Variance (ANOVA) with Welch`s correction and Tamhane`s test. The PS means (MPa) were: 0.60 (G1); 0.47 (G2); 0.52 (G3) and 0.45 (G4), showing significant differences between two photo-activation methods, regardless of the light source used. The continuous method provided the highest values for PS. The data of DC% were analyzed by Analysis of Variance (ANOVA) and shows significant differences for QTH LCUs, regardless of the photo-activation method used. The QTH provided the lowest values for DC%. The gradual method provides lower polymerization contraction, either with halogen lamp or LED. Degree of conversion (%) for continuous or gradual photo-activation method was influenced by the LCUs. Thus, the presented results suggest that gradual method photo-activation with LED LCU would suffice to ensure adequate degree of conversion and minimum polymerization shrinkage.

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The aim of the present study was to evaluate the effects of low-dose therapeutic ionizing radiation on different aesthetic dental materials. Forty five specimens (n = 45) of three different aesthetic restorative materials were prepared and randomly divided into five groups: G1 (control group); G2, G3, G4, G5 experimental groups irradiated respectively with 0.25, 0.50, 0.75, and 1.00 Gy of gamma radiation by the (60)Co teletherapy machine. Chemical analyses were performed using a FT-IR Nicolet 520 spectrophotometer with reflectance diffuse technique. Even a minimal exposition at ionizing radiation in therapeutic doses can provide chemical changes on light-cured composite resins. The three studied restorative materials showed changes after exposure at gamma radiation, however the increase of the radiation dose did not contribute to an increase in this effect.