Para cá e para lá dos muros: relações familiares na interface entre o interior e o exterior da prisão

Tese de Doutoramento em Sociologia

Ashbya gossypii beyond industrial riboflavin production: a historical perspective and emerging biotechnological applications

The filamentous fungus Ashbya gossypii has been safely and successfully used for more than two decades in the commercial production of riboflavin (vitamin B2). Its industrial relevance combined with its high genetic similarity with Saccharomyces cerevisiae together promoted the accumulation of fundamental knowledge that has been efficiently converted into a significant molecular and in silico toolbox for its genetic engineering. This synergy has enabled a directed and sustained exploitation of A. gossypii as an industrial riboflavin producer. Although there is still room for optimizing riboflavin production, the recent years have seen an abundant advance in the exploration of A. gossypii for other biotechnological applications, such as the production of recombinant proteins, single cell oil and flavour compounds. Here, we will address the biotechnological potential of A. gossypii beyond riboflavin production by presenting (a) a physiological and metabolic perspective over this fungus; (b) the molecular toolbox available for its manipulation; and (c) commercial and emerging biotechnological applications for this industrially important fungus, together with the approaches adopted for its engineering.

Comparing different methods to measure biofilm thickness: the techniques are: optical coherence tomography, confocal laser scanning microscopy and low load compression test

Dissertação de mestrado integrado em Engenharia Biomédica (área de especialização em Engenharia Clínica)

Indicadores chave de sustentabilidade para edifícios residenciais em estrutura metálica

Dissertação de mestrado integrado em Engenharia Civil

Why should we expect worlds to collide? - Exploratory reflections departing from a case study analysis of “Jornal de Notícias” (Portugal)

As 2008 came to a close the avalanche of discourse on the demise of newspapers (and traditional media in general) grew to such an extent that consideration of any alternative scenario became almost difficult to utter. Academic articles, conferences, newspaper and magazine features were abundantly produced on thematic variations which went from ‘The End of Newspapers’ to ‘The End of Journalism’ (testing these expressions in a popular search engine we can easily get in excess of 23 thousand references for the first one and over 290 thousand references for the second one and there is even a dedicated ‘Newspaper Death Watch’ site with constant updates). The broad assumption of this production – particularly the one that identifies one possibility with the other – revolves around notions like the collapse of rigid business models, the breakdown of producer/user fidelity/trust, and the failings of a self-centred and entrenched professional (the journalist). The present seems to be enunciated as a ‘the end of days’ period, with images of irrevocable perdition funnelling our reasoning towards one single possible outcome – the imperious necessity of complete reinvention, not necessarily with the same agents.

Mobilidade geográfica dos trabalhadores e implicações processuais

Dissertação de mestrado em Direito Judiciário

O impacto das redes socias na cessação do contrato de trabalho: liberdade de expressão versus justa causa de despedimento

Dissertação de mestrado em Direito e Informática

Citric acid production by Y. lipolytica W29 from crude glycerol: strain improvement by mutagenesis

[Excerpt] Citric acid, an important and versatile organic acid extensively used in several industries, is originally produced by Aspergillus niger in submerged fermentation from molasses [1]. However, Yarrowia lipolytica have been studied and demonstrate a great potential as citric acid producer from several carbon sources [1–5] including crude glycerol, a low cost byproduct from the biodiesel industry [6]. The simultaneous production of the isomer isocitric acid is the major problem in using this yeast in the citric acid production. (...)

Desconformidade como alteração da vontade na venda de bens de consumo

Dissertação de mestrado em Direito dos Contratos e da Empresa