The distribution of penaeid prawns in Ceylon waters

Thirty one species of penaeid prawns have been discovered in Ceylon waters. A taxonomic study of these species was carried out and their distribution in relation to hypothetical oceanographical barriers was discussed (De Bruin 1965.) As penaeid prawns are of important commercial value, a study was made of their distribution and relative abundance in the estuaries and seas round the island. The results of this study are reported in this publication.

Fluctuations in species composition of Penaeid prawns in estuaries

During a study of the distribution of penaeid prawns in Sri Lanka waters a difference was noticed in the species composition from one estuary to another. Moreover, a marked monthly fluctuation in the relative abundance of the different penaied species was also observed. This indicated migratory behaviour. In order to study this phenomenon in the different species of prawns, regular samples of prawns were collected from two estuaries on the south-west coast of Sri Lanka, namely at Moratuwa/Panadura and at Negombo. The investigation was spread over a period of three years from 1957-1959.

State of the stocks of shallow water prawns at Sofala Bank

In 1977 a preliminary assessment of the shallow water prawn (Penaeus indicus ) stocks at Sofala Bank (Mozambique) was made (Ulltang et al., 1980). Using additional data from 1977-1982, the first assessment was updated in 1980 (Ulltang, 1980) and in 1983 (Ulltang et al., 1983). The present report includes a detailed description of data and explanations of the methods used in the last assessment. Data from 1983, which were not available during the last assessment, are also included.

Estimating the population density of prawns by the use of catch per effort data from prawn trawlers at Chilaw

The penaeid prawns of Sri Lanka from estuaries and sea are an important commercial fishery resource. This resource has been exploited over the last century or more by local fishermen using indigenous fishing gear from locally sail-driven or oar-driven fishing crafts. In more recent times, the Fisheries Research Division of the Department of Fisheries undertook surveys of the seas and lagoons of Sri Lanka with a view to ascertain whether any unexploited resources of prawns existed. These publications deal with the species composition, biology, distribution and abundance in the lagoons and inshore waters of Sri Lanka.

Fishery and biology of Penaeid prawns at Harnaii, Mahatashtra

Penaeid prawn fishery at Harnaii in Ratnagiri District of Maharashtra was investigated during fishing seasons of 2002-03 and 2003-04 from mechanized (MLD) and hand operated trawlers (HT). During the two years, MLD contributed 86% and HT 14% to the average annual penaeid prawn catch of 2,242 t. The catch showed two peaks, a major during October-December and a minor during April-May in both the gears but abundance of the individual species differed. P. stylifera, Metapenaeopsis affinis, Solenocera crassicornis, Metapenaeopsis brevicornis, P. merguiensis and Metapenaeopsis dobsoni mainly constituted the fishery and their species composition, seasonal abundance, annual size distribution and monthly mean size were investigated. Biological studies on food, size at maturity, spawning period, sex-ratio and juvenile abundance were carried out to explain temporal abundance of the species in the fishery. Among the species P. stylifera, M. affinis and S. crassicornis exhibited distinct seasonality with two spawning peaks, one in pre-monsoon and the other in post monsoon period to produce two discrete broods while P. merguiensis despite two spawning peaks exhibited a single dominant brood. M. brevicornis showed monsoon and post-monsoon spawning while M. dobsoni showed only post-monsoon spawning. Migrations between nearshore and offshore waters resulted in mixing of the broods and they remained inseparable in the catch.

Comparison of physicochemical properties of muscle proteins in cultured and wild prawns (Penaeus (Fenneropenaeus) orientalis Kishinouye, 1918)

Protein physicochemical properties in cultured and wild prawns (Penaeus (F.) orientalis Kishinouye, 1918) were studied and compared. Protein fractions were separated into water-soluble, salt-soluble, alkali-soluble, and stroma. The results showed that salt- and alkali-soluble proteins were slightly higher in wild prawns and water-soluble proteins were higher in cultured prawns. There were only slight differences in Ca2+-ATPase, MG2+-ATPase, and ATP sensitivities. The textural values of wild prawns were significantly higher than the cultured ones.

ISOLATION AND PROPERTIES OF A BLOOD-COAGULATION FACTOR-X ACTIVATOR FROM THE VENOM OF KING COBRA (OPHIOPHAGUS HANNAH)

A specific blood coagulation factor X activator was purified from the venom of Ophiophagus hannah by gel filtration and two steps of FPLC Mono-Q column ion-exchange chromatography. It showed a single protein band both in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and alkaline polyacrylamide gel electrophoresis. The mol. wt was estimated to be 62,000 in non-reducing conditions and 64,500 in reducing conditions by SDS-PAGE. The isoelectric point was found to be pH 5.6. The enzyme had weak amidolytic activities toward CBS 65-25, but it showed no activities on S-2266, S-2302, thrombin substrate S-2238, plasmin substrate S-2251 or factor Xa substrate S-2222. It had no arginine esterase activity toward substrate benzoylarginine ethylester (BAEE). The enzyme activated factor X in vitro and the effect was absolutely Ca2+ dependent, with a Hill coefficient of 6.83. It could not activate prothrombin nor had any effect on fibrinogen and thus appeared to act specifically on factor X. The procoagulant activity of the enzyme was almost completely inhibited by serine protease inhibitors like PMSF, TPCK and soybean trypsin inhibitor; partially inhibited by L-cysteine. Metal chelator EDTA did not inhibit its procoagulant activity. These results suggest that the factor X activator from O. hannah venom is a serine protease.

Cloning and purification of alpha-neurotoxins from king cobra (Ophiophagus hannah)

Thirteen complete and three partial cDNA sequences were cloned from the constructed king cobra (Ophiophagus hannah) venom gland cDNA library. Phylogenetic analysis of nucleotide sequences of king cobra with those from other snake venoms revealed that obta

Natural diet of penaeid prawns in the coastal waters of Mumbai

Foregut contents of eight commercially important species of penaied prawns namely Penaeus merguiensis, Metapenaeus affinis, M. monoceros, M. brevicornis, Parapenaeopsis stylifera, P. hardwickii, P. sculptilis and Solenocera crassicornis were investigated from inshore, nearshore and offshore fishing grounds of Mumbai. Feeding intensity and index of preponderance (IP) of the dietary items were compared statistically for the species, sexes, fishing areas and maturity condition of females. All the species except M. monoceros and P. sculptilis showed that females were better fed than males. The feeding intensity in the three depth-zones was different for M. affinis, M. brevicornis, M. monoceros, P. hardwickii and S. crassicornis, and uniform for P. merguiensis, P. stylifera and P. sculptilis. Acetes spp., prawn remains, polychaetes, benthic crustaceans, foraminifers and fish remains were the important food items of the prawns. Dietary comparison between the two sexes of the species did not show any difference, but mature females of M. monoceros and P. sculptilis had different diets. Comparison of food items for all the species together showed significant difference between the three areas. Crustacean diet was the favorite in the inshore and nearshore, and polychaetes in the offshore waters. All the species except P. hardwickii showed difference in their dietary composition in the three depth-zones. It is concluded that these coexisting species are primarily carnivorous and exhibit diverse food preferences in different depth-zones by browsing on interstitial organisms, chasing epipelagic prey, raptorial predation, scavenging on dead organisms or adopting different temporal abundance to avoid inter-specific competition for food.

Isolation and cloning of a metalloproteinase from king cobra snake venom

A 50 kDa fibrinogenolytic protease, ohagin, from the venom of Ophiophagus hannah was isolated by a combination of gel filtration, ion-exchange and heparin affinity chromatography. Ohagin specifically degraded the alpha-chain of human fibrinogen and the pr

Molecular characterization of L-amino acid oxidase from king cobra venom

An L-amino acid oxidase from Ophiophagus hannah snake venom (Oh-LAAO) was purified by successive gel filtration, ion-exchange and heparin chromatography. Oh-LAAO did not induce platelet aggregation; however, it had potent inhibitory activity on platelet a

Isolation, expression and characterization of a novel dual serine protease inhibitor, OH-TCI, from king cobra venom

Snake venom Kunitz/BPTI members are good tools for understanding of structure-functional relationship between serine proteases and their inhibitors. A novel dual Kunitz/BPTI serine proteinase inhibitor named OH-TCI (trypsin- and chymotrypsin-dual inhibito

A method for the preparation of cooked-peeled-frozen prawns

Several consignments of cooked-peeled-frozen prawns exported from India were rejected last year due to high total plate count (TPC) at 30°C. The specified temperature of incubation for TPC in our country is 37°C. Hence the effect of incubation at 30 and 37°C on TPC was studied. It is seen that the count is higher on incubation at 30°C. A method for production of cooked-peeled-frozen prawns conforming to the specification for TPC at 30°C is standardized and is reported. It consists of recooking the cooked-peeled prawns followed by packing and freezing without further contamination. The method minimizes batch to batch or sample to sample variation in TPC.

Bacteriology of spoilage of canned prawns

Spoilage characteristics of different types of bacteria isolated from bacteriologically defective cans and processing factory environs were studied by inoculating pure cultures into sterile prawn meat. The pattern of spoilage, namely, production of off odour, bulging of the cans and disintegration of meat were observed. Data on spoilage under aerobic as well as anaerobic conditions are presented. Most of the cultures produced some kind of spoilage, though differences were observed in the extent of spoilage produced by different types of bacteria. Gram positive spore formers were found to be the major spoilers and the extent of spoilage was more with mixed cultures.

Preparation of edible powder from Jawla (Acetes sp.) prawns

Four methods were employed for the preparation of prawn (Acetes) powder in this study. The analytical characteristics and bacteriological quality of the edible powders are presented for each method.