455 resultados para 5S


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通过高温固相法首次合成并报道了兰紫色ZnO Al2O3 SiO2长余辉陶瓷,系统地研究了其发光和缺陷性质。在强度0.6mW·cm-2,主峰254nm的UVP紫外灯下激发15min,然后关闭激发源,样品发射兰紫色长余辉。撤去激发源以后5s,余辉初始强度为230mcd·m-2,色坐标为(0.1292,0.0984)。暗视场中,8h以后余辉仍然肉眼可辨。样品的紫外可见发射和不同时间的余辉发射光谱显示:荧光发射位于390nm,来源于基质的自致发光;而余辉有两个发射峰,主峰位于390nm,肩峰位于520nm。这表明样品中存在两种余辉发射中心。由余辉衰减曲线可以看出,这两种余辉发光都由一个快过程和一个慢过程组成。其中,慢过程决定了材料的长余辉时间。从时间依赖的余辉强度倒数曲线可以看出,余辉强度与时间成反比,这表明余辉发光的机理为电子空穴复合过程。热释光谱显示:样品分别在92和250℃附近出现两个宽的热释峰,说明材料中至少存在两种具有不同陷阱深度的电子或空穴缺陷中心。

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以分子量为550的聚乙二醇单甲醚为侧链,苯乙烯/马来酸酐共聚物为骨架,合成了苯乙烯/马来酸酐共聚物多缩乙二醇酯,用红外光谱、元素分析、DSC、热失重等方法,对合成条件、产物结构和性能进行了研究。结果表明:反应严格按照反应方程进行,精制产物是非晶的梳状聚合物。玻璃化温度为30.68℃,分解温度为120℃。对动态力学性能及其锂盐复合物离子导电性进行研究表明α转变温度和β转变温度分别是28℃和-47.7℃。电导率与温度的依赖关系符合VTF方程。室温电导率最高可达4.2×10-5S/cm。

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用辐射接枝工艺合成了功能高分子膜活性材料,并对其进行了表征,测定了以此为活性材料的离子选择电极性能。结果发现,硫酸根电极在10~(-1)—10~(-3)mol/L Na_2SO_4溶液中响应时间<70s,斜率为52mV/PSO_4~(2-);氯离子电极的线性范围1×10~(-1)—2×10~(-4)mol/L,检测下限为8×10~(-5)mol/L,稳定性好,寿命长,响应时间<5s,内阻<60kΩ,抗毒化能力强。从稀土离子选择电极中发现,以辐射接枝工艺合成的材料比化学法合成的好,前者斜率为53 mV/PGd,后者斜率为46 mV/PGd;预辐照接枝工艺合成的材料比共辐照接枝工艺的好些。

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聚萘是一种新型的高分子,掺杂后具有导电性能,是一种导电聚合物。本文用裂解色谱法(PGC)研究了它的热裂解行为,分离鉴定了主要和特征裂解产物,并在此基础上,对其分子链结构和裂解机理进行了讨论。 实验部分 (一)样品 聚萘(PN):由本实验室直接从萘合成,使用前在100℃下真空热处理48小时加以提纯。 (二)仪器和实验条件 裂解器:美同CDS Py-ro-Probe 190型(丝式);裂解温度300-800℃,裂解时间5s。色谱仪:北分厂-2305E型,氢火焰离子化检

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聚乙快的不稳定性及难以加工影响了其应用.最近,Naarmann通过陈化在硅油中的Ti(OBu)_4~-AlEt_3催化体系及加入不同量的Li-(n-Bu)制得了拉伸后电导率达10~5S/cm的聚乙炔膜,此膜的电导和形态在空气中几个月几乎不发生改变.我们曾报导用加热的甲苯-Ti(OBu)_4~--AlEt_3催化体系合成高性能聚乙炔膜.本文进一步讨论不同溶剂对高性能聚乙炔(HPPA)合成及性能的影响.

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稀土离子4f轨道是否参与成键是一个引入注目的课题,多年来一直存在不同见解。一种认为不参与成键,理由是由于5s,5p壳层的屏蔽,4f轨道位于内壳层,并且到目前为止,分子轨道理论计算结果表明4f轨道不参与成键。另一种认为可以形成较弱的共价键,并发展了一种角重迭模型利角参数来研究共价行为,但是具体共价行为的数量级尚未有明确结论。我们在

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Cultivation of the endophytic fungus Penicillium commune, which was isolated from the semi-mangrove plant Hibiscus tiliaceus, afforded one new compound 1-O-(2,4-dihydroxy-6-methylbenzoyl)-glycerol (1) along with thirteen known products, including 1-O-acetylglycerol (2), N-acetyltryptophan (3), 3-indolylacetic acid methyl ester (4), 1-(2,4-dihydroxy-3,5-dimethylphenyl)ethanone (5), 2-(2,5-dihydroxyphenyl)acetic acid (6), (4R,5S)-5-hydroxyhexan-4-olide (7), thymidine (8), uracil (9), thymine (10), ergosterol (11), beta-sitosterol (12), beta-daucosterol (13), and ergosta-7,22-dien-3 beta,5 alpha,6 beta-triol (14). The structures of these compounds were established by detailed NMR spectroscopic analysis, as well as by comparison with literature data or with authentic samples.

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本研究将荧光原位杂交(Fluorescence in situ hybridization, FISH)技术引入中国明对虾和栉孔扇贝这两种重要的海水养殖动物的染色体研究中,建立了相关技术平台,在染色体上定位了部分功能基因,详述如下: 1. 在中国明对虾和栉孔扇贝中建立了FISH平台,利用单色和双色FISH技术在中国明对虾和栉孔扇贝染色体上成功定位了多拷贝基因,发现5S rDNA定位于中国明对虾的一对同源染色体上,栉孔扇贝18S rDNA和组蛋白序列也各自定位于一对同源染色体上,它们均可以作为染色体特异性探针来鉴别染色体。 2. 在栉孔扇贝中发展了BAC-FISH技术,定位了包含热休克蛋白70(HSP70)、丝氨酸蛋白酶(Serine Protease)和脂多糖葡聚糖结合蛋白(LGBP)等免疫相关基因的BAC克隆,发现它们均定位于一对同源染色体的长臂上。利用双色BAC-FISH技术,发现6个包含栉孔扇贝LGBP基因的BAC克隆在间期细胞核上共定位。对栉孔扇贝5个探针进行了同时定位,初步鉴别了栉孔扇贝5条染色体。 3. 在栉孔扇贝热休克蛋白70(HSP70)、丝氨酸蛋白酶(Serine Protease)和脂多糖葡聚糖结合蛋白(LGBP)等免疫相关基因内部发掘了数个潜在的SNP位点,展示了栉孔扇贝SNP位点的丰富性。这些SNP位点可以用于图谱整合。

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本研究应用显带技术和荧光原位杂交(Fluorescence in situ hybridization,FISH)技术,鉴定了牡蛎的染色体;应用FISH方法定位了一系列的重复序列和大分子的P1克隆DNA;制备了染色体特异性探针。应用FISH特异性探针成功地鉴定了长牡蛎的三体10。结果如下:1.分析了G带和C带在美洲牡蛎染色体上的分布。G带在每一条染色体上的带型不同,某些染色体间(如第1对和第4对染色体,第7对和第9对染色体)的带型差别不是很明显。G带型容易受染色体收缩程度的影响。C带型重复性较好,染色体带型较清楚,分布在染色体的端粒区域和着丝粒区域。G带和C带带型能够用来鉴定牡蛎的染色体,但是重复性低和带型差异不显著,并不适合常规的染色体鉴定。2.早期胚胎和担轮幼虫制备的染色体适合于FISH分析。染色体制备方法重复性好,可适用于其它贝类的染色体制备。3.研究了重复序列基因--rDNA的定位:1)18S-5.8S rDNA在研究的五种巨蛎属Crassostrea牡蛎均只有一个位 点。太平洋种(C.gigas,C. ariakensis和C. plicatula)中,杂交信号位于最短的染色体一第10对染色体长臂的端粒区域,在大西洋种(C. virginica和C. rhizophorae)中,同一序列定位在第2对染色体短臂的端粒区域。2)18S-28S rDNA在两种蛤中有两个位点。rDNA探针定位在侏儒蛤(Mulinis Lateralis)的第15对和第19对染色体的端粒区域,同一序列定位在硬壳蛤(Mercenaria mercenaria)的第10对染色体的长臂和第12对染色体短臂的端粒区域。信号强度在两对染色体之间有差异。 3)5s rDNA位于美洲牡蛎的第5对染色体的短臂上靠近着丝粒区域和第6 对染色体的短臂的中间区域。信号强度在两对染色体之间没有显著差异。5S rDNA探针可以作为鉴定和识别第5对和第6对染色体的特异性探针。4.研究了一些重复序列的定位1)两个短的重复序列1G8,1P2均产生很强的荧光信号分布在美洲牡蛎所有的染色体上。在低严谨条件下,这些序列均产生很强的信号散布在所有的染色体上。在高严谨条件下,信号强度大大减弱,但是信号仍散布在所有的染色体上。这些重复序列散布在美洲牡蛎的整个基因组中。2)高度重复序列Cgl70产生的信号分布在长牡蛎的7对染色体的着丝粒区域,没有发现间区信号。在第1对,第2对,第4对和第7对染色体上的荧光信号强且稳定。在第5对,第8对和第10对染色体上的信号相对弱且不稳定。在剩余的染色体上(第3对,第6对和第9对染色体)没有检测到荧光信号。结果表明此卫星序列是一个着丝粒卫星序列。在美洲牡蛎的染色体上没有检测到荧光信号,表明了这个着丝粒卫星序列在这两种牡蛎中的分布存在着显著的差异。3)脊椎动物端粒序列(TTAGGG)n的FISH信号局限在四种双壳贝类(美洲牡蛎,the mangrove oyster,硬壳蛤,侏儒蛤)所有染色体的端粒区域,没有发现间区信号的存在。研究结果与已报道的研究结果表明脊椎动物端粒序列或许存在于所有双壳贝类的染色体末端。双壳贝类是目前研究过的唯一含有脊椎动物端粒序列DNA的无脊椎动物。4)研究了RAPD探针在美洲牡蛎染色体上的定位。大多数RAPD探针产生了多个信号散布在间期细胞核和所有的染色体上。引物OPX-03,OPX-04,OPX—06,OPG-02,OPM—04,OPM-11,0PS-02制备的探针在适宜的条件下产生特异性荧光 信号,分布在牡蛎的特定的染色体上。PCR特异性带产生的探针OPX—06—310和0PG-02—300产生了特异性的荧光信号:OPX—06—310产生的信号位于第5对染色体的短臂的近端粒区域,0PG—02—300探针定位到第3对染色体的短臂上。这两个探针是鉴定美洲牡蛎单条染色体的特异性探针。5.研究了大分子Pl克隆DNA(插入片断为80~100 kb)在美洲牡蛎染色体上的定位。Pl克隆DNA通过切口平移方法标记digoxigenin—11-dUTP用作FISH的探针。Cot-1 DNA作为竞争剂有效地抑制了Pl克隆序列中的重复序列产生的信号。杂交信号用fluorescein标记的anti—digoxigenin抗体来检测,用两层抗体rabbit-anti-sheep抗体和FITC anti—rabbit抗体来扩增信号。9个P1探针成功地定位在特定的染色体上。46—1探针杂交到第1对染色体的长臂靠近着丝粒区域;47-10探针定位到第2对染色体的长臂近端粒区域;Cvpl和48-13两探针定位到第3对染色体上:Cvpl位于短臂的端粒区域,48-13探针位于长臂的近着丝粒区域;48—10探针杂交到第4对染色体的长臂上;48-1探针杂交到第5对染色体长臂的近着丝粒区域;49-11探针位于第7对染色体长臂上;探针49-10和44-11位于第8对染色体长臂上。同时我们成功地将2个P1探针杂交到同一染色体分裂相中,进一步确定了Pl探针在美洲牡蛎染色体 上的定位。6.应用18S-28S rDNA探针成功地鉴定出长牡蛎非整倍体中的三体10。经鉴定AF-35,AF-39和AF-3三体家系属于三体10家系。rDNA探针分布在三条染色体上,即多出的一条染色体为染色体10。相应地在间期细胞核上有三个信号出现。AF-34和AF-36家系不属于三体10家系。rDNA探针分布在两条染色体上,相应地在间期细胞核上有两个信号出现。FISH和染色体特异性探针为非整倍体的鉴定提供了一个快速准确可靠的方法和途径。

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海藻是海洋生物中的一大类群,由于其特殊的生活环境,能够代谢产生大量结构独特多变和活性特殊多样的代谢产物,是化学和生物活性多样性研究的重要对象之一。我国海域辽阔,海藻资源丰富,为寻找结构新颖、生理活性独特的先导化合物,加强对海藻资源的开发利用,本论文对中国沿海的三种海洋红藻进行了化学成分和生物活性研究,同时对山东青岛海域生物量丰富的一种海洋红藻松节藻进行了动物体内抗糖尿病活性研究。 利用正相硅胶柱色谱、Sephadex LH-20柱色谱以及反相HPLC和重结晶等现代分离手段,对山东青岛沿海的红藻扇形叉枝藻(Gymnogongrus flabelliformis)进行了系统的化学成分研究,从中得到单体化合物26个,通过波谱学方法(IR、MS、NMR等)鉴定了他们的结构,分别为(3R,6R,7E)-(+)-3-O-phenylacetyl- 4,7-megastigmadiene-9-one(1),(3R,7E)-(-)-3-O-phenylacetyl-5,7-megastigmadiene -9-one(2),(3S,6R,7E)-(+)-3-hydroxyl-4,7-megastigmadien-9-one(3),(3S,5R,6S,7E)- (-)-3-hydroxy-5,6-epoxy-7-megastigmene-9-one(4),(3S,5S,6R,7E)-(+)-3-hydroxy- 5,6-epoxy-7-megastigmene-9-one(5),Dehydrovomifoliol(6),(3R)-(-)-4-[(2R,4S)-4- acetoxy-2-hydroxy-2,6,6-trimethylcyclohexylidene]-3-buten-2-one(7),2,3,3′-三溴-4,4′,5,5′-四羟基-1′-乙氧甲基双苯基甲烷(8),2,2′,3,3′-四溴-4,4′,5,5′-四羟基双苯基甲烷(9),3-溴-4,5-二羟基苯甲醛(10),2,3-二溴-4,5-二羟基苯甲基甲醚(11),2,3-二溴-4,5-二羟基苯甲醇(12),N, N-二甲基酪胺(13),4-羟基苯甲酸乙酯(14),4-羟基苯甲基乙醚(15),4-羟基苯乙基乙酯(16),4-羟基苯乙酸甲酯(17),4-羟基苯甲醛(18),豆甾-4-烯-3-酮(19),胆甾-4-烯-3-酮(20),胆甾醇(21),尿嘧啶(22),尿嘧啶核苷(23),腺嘌呤核苷(24),丁二酸(25),5-羟基-4-甲基-5-戊基-2,5-二氢呋喃-2-酮(26)。其中化合物1、2为新化合物,化合物3为新天然产物,所有化合物均为首次从该属海藻中分离得到。通过 MTT 法对部分单体化合物进行了肿瘤细胞毒活性筛选, 结果表明,化合物8、9、10、12对筛选的所有细胞株均有较强细胞毒活性,化合物11对人肺癌细胞株(A549)、人肝癌细胞株(Bel 7402)、人结肠癌细胞株(HCT-8)有一定细胞毒活性。通过研究单体化合物对小鼠腹腔巨噬细胞TNF-分泌的影响,对其进行抗炎活性筛选,结果表明,化合物8、9、11、13、17、23、24、25对小鼠腹腔巨噬细胞TNF-分泌表现出明显的抑制作用。 从采自山东荣成镆铘岛的红藻小珊瑚藻(Corallina pilulifera)的乙酸乙酯萃取物中分离得到16个单体化合物,通过波谱学方法鉴定化合物结构14个(另外2个正在鉴定中),分别为2α-乙氧酰基-2β-羟基-A-降胆甾-5-烯-4-酮(27),胆甾-4-烯-3-酮(28),胆甾醇(29),3β-羟基-胆甾-5,24(28)-二烯-7-酮(30),2α-羟基-胆甾-4-烯-3-酮(31),6α-羟基-胆甾-4-烯-3-酮(32),3β-羟基-胆甾-5-烯-7-酮(33),(E)-phytol epoxide(34),Phytenal(35),3,7,11,15- tetramethyl-hexadec-2-en-1-oll(Phytol)(36),Loloilide(37),(3S,5R,6S,7E)-(-)-3-hydroxy-5,6-epoxy-7- megastigmene-9-one(38),Dehydrovomifoliol(39),4-羟基苯甲醛(40)。其中,化合物 31为新天然产物,化合物27为首次从植物中分离得到,所有化合物均为首次从该种海藻中分离得到。通过 MTT 法对分离得到的单体化合物进行了肿瘤细胞毒活性筛选,化合物27和化合物32对筛选的所有肿瘤细胞株均有细胞毒活性,且化合物27对人胃癌细胞株(BGC-823)、人结肠癌细胞株(HCT-8)和人卵巢癌细胞株(A2780)具有中等强度抑制活性。化合物28、化合物31和化合物33对人肝癌细胞株(Bel 7402)、人结肠癌细胞株(HCT-8)和人卵巢癌细胞株(A2780)有一定细胞毒活性。 从采自广西北海涠洲岛的多管藻Polysiphonia sp.的乙酸乙酯萃取物中分离得到6个单体化合物,通过波谱学方法鉴定化合物结构5个(另外1个仍在鉴定),分别为胆甾醇(41),3,7,11,15-tetramethyl-hexadec-2-en-1-ol(Phytol)(42),3-吲哚甲醛(43),4-羟基苯甲醛(44),4-羟基苯甲酸(45)。 对山东青岛沿海的松节藻 (Rhodomela confervoides) 乙醇提取物进行了初步的体内抗糖尿病活性研究,采用链脲佐菌素诱导的2型糖尿病(STZ-DM)大鼠模型对其进行体内降糖实验,结果发现,松节藻乙醇提取物在糖尿病大鼠体内不仅具有显著的降血糖作用,且呈现良好的量–效关系,而且能够纠正糖尿病引发的物质代谢紊乱,增加体重,提高试验动物的成活率,因此具有良好的应用开发前景。

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Seven new cadinane sesquiterpenes, (-)-(1R,6S,7S,10R)-1-hydroxycadinan-3-en-5-one (1), (+)-(1R,5S,6R,7S, 10R)-cadinan-3-ene-1,5-diol (2), (+)-(1R,5R,6R,7S,10R)-cadinan-3-ene-1,5-diol (3), (+)-(1R,5S,6R,7S,10R)-cadinan-4(11)-ene-1,5-diol (4), (+)-(1R,5R,6R,7R,10R)-cadinan-4(11)-ene-1,5,12-triol (5), (-)-(1R,4R,5S,6R,7S, 10R)-cadinan-1,4,5-triol (6), and (-)-(1R,6R,7S,10R)-11-oxocadinan-4-en-1-ol (7), together with nine known compounds were isolated from the brown alga Dictyopteris divaricata. The structures of the new natural products, as well as their absolute configuration, were established by means of spectroscopic data including IR, HRMS, 1D and 2D NMR, single-crystal X-ray diffraction, and CD. All compounds were inactive against several human cancer cell lines including lung adenocarcinoma (A549), stomach cancer (BGC-823), breast cancer (MCF-7), hepatoma (Bel7402), and colon cancer (HCT-8) cell lines.

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Five minor sesquiterpenes (1-5) with two novel carbon skeletons, together with a minor new oplopane sesquiterpene ( 6), have been isolated from the brown alga Dictyopteris divaricata. By means of spectroscopic data including IR, HRMS, 1D and 2D NMR, and CD, their structures including absolute configurations were assigned as (+)-(1R, 5S, 6S, 9R)3- acetyl-1-hydroxy-6-isopropyl-9-methylbicyclo[4.3.0] non-3-ene ( 1), (+)-(1R, 3S, 4S, 5R, 6S, 9R)-3-acetyl-1,4-dihydroxy-6- isopropyl-9-methylbicyclo[4.3.0] nonane (2), (+)-(1R, 3R, 4R, 5R, 6S, 9R)-3-acetyl-1,4-dihydroxy-6-isopropyl-9-methylbicyclo[ ;4.3.0] nonane ( 3), (+)-(1S, 2R, 6S, 9R)-1-hydroxy-2-(1-hydroxyethyl)-6-isopropyl-9-methylbicyclo[4.3.0] non-4-en-3-one (4), (-)-( 5S, 6R, 9S)-2-acetyl-5-hydroxy-6-isopropyl-9-methylbicyclo[4.3.0] non-1-en-3-one ( 5), and (-)-( 1S, 6S, 9R)- 4-acetyl- 1-hydroxy-6-isopropyl-9-methylbicyclo[ 4.3.0] non-4-en-3-one ( 6). Biogenetically, the carbon skeletons of 1-6 may be derived from the co-occurring cadinane skeleton by different ring contraction rearrangements. Compounds 1-6 were inactive (IC50 > 10 mu g/mL) against several human cancer cell lines.

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Three bisnorsesquiterpenes (1-3) with novel carbon skeletons and a norsesquiterpene (4) have been isolated from the brown alga Dictyopteris divaricata. By means of spectroscopic data including IR, HRMS, 1D and 2D NMR techniques, single-crystal X-ray diffraction, and CD, their structures including absolute configurations were proposed as (+)-1R,6S,9R)-1-hydroxyl-6-isopropyl-9-methylbicyclo[4.3.0]non-4-en3-one (1), (-)-(1S,6S,9R)-1-hydroxyl-6-isopropyl-9-methylbicyclo[4.3.0] non-4-en-3-one (2), (+)-(5S,6R,9S)5-hydroxyl-6-isopropyl-9-methylbicyclo [4.3.01 non-1-en-3-one (3), and (-)-(1R,7S,10R)-1-hydroxy-1lnorcadinan-5-en-4-one (4). Biogenetically, the carbon skeleton of 1-3 may be derived from the co-occurring cadinane skeleton by ring contraction and loss of two carbon units, and compound 4 from the oxidation of cadinane derivatives. Compounds 1-4 were inactive (IC50 > 10 mu g/mL) against several human cancer cell lines including lung adenocarcinoma (A549), stomach cancer (BGC-823), breast cancer (MCF-7), hepatoma (Bel7402), and colon cancer (HCT-8) cell lines.

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Karyotype and chromosomal localization of major (18-5.8-28S) and minor (5S) ribosomal RNA genes were studied in two species of Pectinidae, zhikong (Chlamys farreri) and bay (Argopecten irradians irradians) scallops. using fluorescence in situ hybridization (FISH). C. farreri had a haploid number of 19 with a karyotype of 3m + 4sm + 7sm-st + 4st + 1st-t, and A. i. irradians had a haploid number of 16 with a karyotype of 5st + 11t. In C. farreri, the major and minor rRNA genes had one locus each and were mapped to the same chromosome-Chromosome 5. In A. i. irradians, the major rRNA genes had two loci, located on Chromosomes 4 and 8, and the 5S rRNA gene was found at a third chromosome-Chromosome 10. Results of this and other studies indicate that karyotype of A. i. irradians (n = 16, 21 arms) is secondary and derived from an ancestral karyotype similar to that of C. farreri (n = 19, 38 arms) through considerable chromosomal loss and rearrangements. The ability to tolerate significant chromosomal loss suggests that the modal karyotype of Pectinidae and possibly other bivalves with a haploid number of 19 is likely tetraploid; i.e., at least one genome duplication has occurred during the evolution of Bivalvia.

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Our study deals with the high resolution body wave tomography in North china and adjacent areas(30°N-43°N,100°E-130°E), where earthquakes occurred many times in history and has a very complicated geological structure. 6870 events recorded at 273 digital seismic stations from CDSN during 1996-2002 and stations settled by Seislab of IGCAS in Bohai Bay area, including 1382 local earthquakes and 5488 teleseismic earthquakes are used in this study. In the data we used, the average number of received stations is greater than 5, the error of picking up direct arrival time is 0.1-0.5s. Before the inversion, we use Checkerboard method to confirm the reliability of result of Local events; use Restoring Resolution Test to confirm the reliability of result of teleseismic events. We also analyzed the effect of different parameters in the inversion. Based the analysis above, the model used in this paper is divided into small blocks with a dimension of 0.33°in the latitude and longitude directions and 5km、15km、30km in depth, and initial velocity model. Using pseudobending method to calculate the ray traveling path, LSQR algorithm to inverse, finally, we got the body velocity images below 25km and above 480km in this area using Joint- inversion with local events and teleseismic events. We made the conclusion at last: (1)at top zone of the south of Sichuan Basin , there exits low velocity anomalies, below 40km is the high velocity zone extend to 300km; (2) Above the 40km of Ordos block exits low velocity zone, while below 40km until 240km, the high velocity anomalies are interlaced by low velocity anomalies. Below 300km, the anomalies are unclear any more; (3) On the whole, the velocity structure below 400km on the mantle transition zone of Eastern China area shows its changes from low velocity to high velocity.