Las rutas alimentarias, herramienta para valorizar productos de las agroindustrias rurales. El caso de la ruta del queso Turrialba, Costa Rica

En la búsqueda de estrategias para el desarrollo de las zonas rurales, aparece la actividad del turismo como un instrumento de aplicación práctica que puede contribuir a generar procesos de desarrollo local que permitan complementar los ingresos agrícolas, crear nuevos puestos de empleo y evitar el despoblamiento rural.   El agroturismo y las rutas alimentarias entendidas como modalidades del turismo rural resultan de especial interés porque representan nuevas actividades en el medio rural que agregan valor a las producciones agroalimentarias en manos de pequeños productores y en especial a aquellas que emplean procesos artesanales de elaboración.   En el actual contexto de apertura comercial gobernada por los commoditties, el fast-food y las barreras sanitarias, la existencia de muchas pequeñas agroindustrias rurales se ve amenazada puesto que no alcanzan los niveles de productividad para competir en mercados globalizados. Muchas de esas empresas podrían tener una salida si logran articularse con el negocio del turismo rural, por medio de la prestación de productos y servicios para atender las demandas de los turistas interesados en conocer aspectos de la cultura agropecuaria y agroindustrial de una determinada región. Esta propuesta nos lleva a analizar las características generales del turismo rural, el agroturismo y las rutas alimentarias, así como la manera en que esos conceptos pueden aplicarse en un territorio específico, para potenciar procesos y productos con identidad, como es el caso del queso Turrialba en Costa Rica, alrededor del cual se propone la construcción de una ruta alimentaria como estrategia de desarrollo local y valorización de la agroindustria quesera artesanal.   ABSTRACT This paper takes us to analyze the general characteristics of rural tourism, agro-tourism and food routes and of how those concepts may be applied in specific territories, to promote processes and develop products with territories, to promote processes and develop products with territorial identity, as is the case of the Turrialba cheese in Costa Rica. Tourism appears to be an instrument of practical application that can contribute to generate processes of local development that helps to complement agricultural incomes, create new employments and avoid rural migration. Agro-tourism and food routes are of special interest since they represent new activities in the rural areas which add value to the agro-alimentary productions through the hands of small producers.

Studies of MHC class I antigen presentation & the origins of the immunopeptidome

La présentation d'antigène par les molécules d'histocompatibilité majeure de classe I (CMHI) permet au système immunitaire adaptatif de détecter et éliminer les agents pathogènes intracellulaires et des cellules anormales. La surveillance immunitaire est effectuée par les lymphocytes T CD8 qui interagissent avec le répertoire de peptides associés au CMHI présentés à la surface de toutes cellules nucléées. Les principaux gènes humains de CMHI, HLA-A et HLA-B, sont très polymorphes et par conséquent montrent des différences dans la présentation des antigènes. Nous avons étudié les différences qualitatives et quantitatives dans l'expression et la liaison peptidique de plusieurs allotypes HLA. Utilisant la technique de cytométrie de flux quantitative nous avons établi une hiérarchie d'expression pour les quatre HLA-A, B allotypes enquête. Nos résultats sont compatibles avec une corrélation inverse entre l'expression allotypique et la diversité des peptides bien que d'autres études soient nécessaires pour consolider cette hypothèse. Les origines mondiales du répertoire de peptides associés au CMHI restent une question centrale à la fois fondamentalement et dans la recherche de cibles immunothérapeutiques. Utilisant des techniques protéogénomiques, nous avons identifié et analysé 25,172 peptides CMHI isolées à partir des lymphocytes B de 18 personnes qui exprime collectivement 27 allotypes HLA-A,B. Alors que 58% des gènes ont été la source de 1-64 peptides CMHI par gène, 42% des gènes ne sont pas représentés dans l'immunopeptidome. Dans l'ensemble, l’immunopeptidome présenté par 27 allotypes HLA-A,B ne couvrent que 17% des séquences exomiques exprimées dans les cellules des sujets. Nous avons identifié plusieurs caractéristiques des transcrits et des protéines qui améliorent la production des peptides CMHI. Avec ces données, nous avons construit un modèle de régression logistique qui prédit avec une grande précision si un gène de notre ensemble de données ou à partir d'ensembles de données indépendants génèrerait des peptides CMHI. Nos résultats montrent la sélection préférentielle des peptides CMHI à partir d'un répertoire limité de produits de gènes avec des caractéristiques distinctes. L'idée que le système immunitaire peut surveiller des peptides CMHI couvrant seulement une fraction du génome codant des protéines a des implications profondes dans l'auto-immunité et l'immunologie du cancer.

Firm-level trade creation and diversion of regional trade agreements in Thailand

Using highly detailed import data for Thailand, this paper examines firm-level trade creation and diversion of regional trade agreements (RTAs). Specifically, by focusing on firm-product pairs in which firms import a particular product from non-members but not from RTA members in the initial year of our sample, we empirically investigate the start of imports from RTA members under RTA schemes and the cessation of imports from non-members at the firm-level. We find that firms are more likely to stop importing products with low RTA tariff rates or high most-favored-nation tariff rates from non-members and to start importing such products from RTA member countries. However, from the quantitative point of view, there are very few firms that switch import sources from non-members to RTA members when facing the introduction of RTA schemes.

Studies of MHC class I antigen presentation & the origins of the immunopeptidome

La présentation d'antigène par les molécules d'histocompatibilité majeure de classe I (CMHI) permet au système immunitaire adaptatif de détecter et éliminer les agents pathogènes intracellulaires et des cellules anormales. La surveillance immunitaire est effectuée par les lymphocytes T CD8 qui interagissent avec le répertoire de peptides associés au CMHI présentés à la surface de toutes cellules nucléées. Les principaux gènes humains de CMHI, HLA-A et HLA-B, sont très polymorphes et par conséquent montrent des différences dans la présentation des antigènes. Nous avons étudié les différences qualitatives et quantitatives dans l'expression et la liaison peptidique de plusieurs allotypes HLA. Utilisant la technique de cytométrie de flux quantitative nous avons établi une hiérarchie d'expression pour les quatre HLA-A, B allotypes enquête. Nos résultats sont compatibles avec une corrélation inverse entre l'expression allotypique et la diversité des peptides bien que d'autres études soient nécessaires pour consolider cette hypothèse. Les origines mondiales du répertoire de peptides associés au CMHI restent une question centrale à la fois fondamentalement et dans la recherche de cibles immunothérapeutiques. Utilisant des techniques protéogénomiques, nous avons identifié et analysé 25,172 peptides CMHI isolées à partir des lymphocytes B de 18 personnes qui exprime collectivement 27 allotypes HLA-A,B. Alors que 58% des gènes ont été la source de 1-64 peptides CMHI par gène, 42% des gènes ne sont pas représentés dans l'immunopeptidome. Dans l'ensemble, l’immunopeptidome présenté par 27 allotypes HLA-A,B ne couvrent que 17% des séquences exomiques exprimées dans les cellules des sujets. Nous avons identifié plusieurs caractéristiques des transcrits et des protéines qui améliorent la production des peptides CMHI. Avec ces données, nous avons construit un modèle de régression logistique qui prédit avec une grande précision si un gène de notre ensemble de données ou à partir d'ensembles de données indépendants génèrerait des peptides CMHI. Nos résultats montrent la sélection préférentielle des peptides CMHI à partir d'un répertoire limité de produits de gènes avec des caractéristiques distinctes. L'idée que le système immunitaire peut surveiller des peptides CMHI couvrant seulement une fraction du génome codant des protéines a des implications profondes dans l'auto-immunité et l'immunologie du cancer.

Uso de literatura infantil para o desenvolvimento de capacidades emocionais

Dissertação de Mestrado apresentada no ISPA – Instituto Universitário para obtenção de grau de Mestre na especialidade de Psicologia Educacional

Synthetic studies towards the total synthesis of hexacyclinic acid

In the first chapter of this thesis, published works found in the literature about hexacyclinic acid and FR182877 are reported and commented. A quick summary of the previous work done in the Prunet group is also described. In the second and third chapter, a more detailed account of the work undertaken during this PhD was given. Firstly, syntheses of two ABC tricycles incorporating tert-butyl and (trimethylsilyl)ethyl esters were undertaken. These syntheses include two key steps previously developed in the group, a diastereoselective Michael addition and a Snider cyclisation. Multiple conditions for the hydrolysis of the esters were attempted but none of them gave the desired product. The main part of this work is focused on the synthesis of a CDEF model and in particular about the development of the key step, the formation of a nine-membered ring. Several DEF fragments were synthesised in short synthetic sequences and as single isomers. Six different synthetic pathways were developed in total and a novel method, a Michael/elimination reaction, was found to be a very efficient way to close the desired medium-size ring. From the nine-membered ring, regioselective reduction and palladiumcatalysed allylic substitution led to the formation of the CDF tricycle. Final steps of the synthesis were fruitless and led only to decomposition. A synthesis of a chiral C-ring was also developed during this PhD. II Finally, another project was undertaken, not related to hexacyclinic acid. Methodology developed in the group for the diastereoselective formation of trisubstituted alkenes employing a temporary silicon-tethered ring-closing metathesis was extended to homoallylic alcohols. The first steps of the method were similar to the previous methodology but the end-game had to be modified in favour of an oxidation/reduction sequence to successfully obtain the desired products with the correct geometry. In the fourth chapter, procedures and analytical data for the synthesised compounds previously described are reported.

Evaluación de los contenidos de información de medicamentos en un grupo de agencias reguladoras de medicamentos

OBJETIVO: Evaluar las diferencias de información publicada por el Invima y otras Autoridades Reguladoras de Medicamentos en relación a un grupo de variables seleccionadas y analizar las diferencias aplicando estrategias de visualización de datos. METODOLOGÍA: Estudio descriptivo de corte transversal, en el cual se comparan las diferencias en la información de medicamentos publicada en las agencias reguladoras de medicamentos. Para la definición de los criterios de comparación, más relevantes se utilizó la técnica Delphi como metodología de consenso. Las visualizaciones fueron desarrolladas en Roassal, en el entorno de programación de Grafoscopio/Pharo. RESULTADOS Y CONCLUSIONES: De 24 variables en las que hubo acuerdo de considerarlas importantes y claves por el panel de expertos; el 96% se identificaron en la Aemps, MHRA e Infarmed; el 92% en Anmat, TGA, Anvisa, Health Canada y FDA; el 80% en Anamed. El grupo de agencias en las que se identificó una menor cantidad de información publicada fueron Cofepris, Invima, Dirección Nacional de Farmacias y Drogas de Panamá, Digemid, Arcsa y el Ministerio de Salud pública de Uruguay en las que se identificó el 67%, 58%, 46%, 46%, 38% y 4%, respectivamente. Las ausencias comunes de información en el grupo de agencias con menor cantidad de información se presentó en las variables relacionadas con usos fuera de indicación (off-label), gestión del riesgo, efectos adversos, restricciones especiales, precauciones y advertencias, prospecto, almacenamiento y resumen de las características del producto, es decir que mayor la limitación se presenta en la información relacionada con prescripción y uso.

Plan de negocio Aprisco JAM

El Aprisco JAM busca entrar en el mercado de lácteos y derivados con un producto que posee atributos nutricionales importantes al igual que provee la facilidad de ser consumido por cualquier persona, principalmente aquellas que no toleran la leche vacuna encontrando en nuestros productos una alternativa para el disfrute de estos sin comprometer su salud y bienestar. Encontramos así pues una demanda existente en donde ofrecemos como solución a las necesidades un producto nutritivamente saludable siendo recordados y diferenciados por este aspecto. Todo ello en base a una producción limpia y sostenible que permita un aprovechamiento y uso eficiente de los recursos naturales. Para lograr dicho objetivo es necesario una inversión inicial de $24.742.036 COP, proyectado a 3 años dicha inversión se recuperará a partir del 5 mes con un interés esperado de 35,6% EA que representa beneficios en valor actual de $90´628.858. Por otro lado genera una relación beneficio-costo de 4,66, esto quiere decir que por cada peso invertido se generan beneficios de 3,66 pesos.

Clínica médica e cirúrgica em animais de companhia

O presente relatório foi realizado no âmbito do Mestrado Integrado de Medicina Veteri-nária, estando divido em duas partes. A primeira parte refere-se à casuística acompanhada ao longo do estágio curricular, realizado no Centro Hospitalar Veterinário do Porto, no período de-corrido entre 1 de setembro de 2015 e 29 de fevereiro de 2016. A segunda parte engloba uma monografia sobre o tema “Cetoacidose Diabética em canídeos”, incluindo ainda o relato de três casos clínicos acompanhados no decorrer do estágio. A cetoacidose diabética (CAD) é uma complicação urgente do diabetes mellitus, ocorrendo principalmente em animais nunca antes tratados com insulina de longa ação. O diagnóstico de CAD passa pela anamnese, sinais clínicos e deteção de hiperglicemia severa, glicosúria e cetonúria. O diagnóstico e tratamento de doenças concomitantes constituem o passo mais desafiante. O prognóstico depende da severidade da acidose, das doenças concomitantes e das limitações financeiras dos proprietários; Abstract: Small Animal Practice This report was carried out for the completion of the Master degree in veterinary medicine and is divided in two parts. The first part includes the accompanied cases throughout the train-eeship, which was held at the Centro Hospitalar Veterinário of Porto, between September 1st 2015 and February 29th 2016. The second part includes a monograph on "Diabetic Ketoacidosis in dogs ", including the report of three clinical cases followed during the internship. Diabetic ke-toacidosis (DKA) is an urgent complication of diabetes mellitus, occurring mainly in animals that were never treated with long-action insulin. The diagnosis of DKA involves the history, clinical signs and detection of severe hyperglycemia, glycosuria and ketonuria. The diagnosis and treat-ment of concomitant diseases are the most challenging steps. The prognosis depends on the severity of acidosis and concomitant diseases, as well as the financial restrictions of the owners.

Prática de ensino supervisionada em Pré-escolar e em 1.º ciclo do ensino básico: organização de ambientes educativos promotores da educação em ciências naturais

O presente relatório, elaborado no âmbito Prática de Ensino Supervisionada em Pré-Escolar e em 1.º Ciclo do Ensino Básico dá a conhecer os processos de aprendizagem imanentes à prática desenvolvida em contexto. Em Educação Pré- Escolar elaborei um memorial de formação que permitiu refletir sobre as experiências vividas no estágio, possibilitando a aquisição de conhecimentos e aprendizagens profissionais. No 1º Ciclo do Ensino Básico o estágio também assumiu uma perspetiva reflexiva que me permitiu aprender a profissão, mas a dinâmica do trabalho com as crianças possibilitou, igualmente, a realização de um projeto alinhado com a investigação-ação. Os contextos foram diferenciados pela sua especificidade, quer pela situação geográfica e cultural das instituições, quer pelo nível de ensino em que se desenvolveram. A prática educativa em 1º Ciclo do Ensino Básico ocorreu na escola EB/JI Galopim de Carvalho em Évora, já a prática em Pré-Escolar ocorreu no Núcleo de Educação Infantil Colônia Z-11, na cidade de Florianópolis no Brasil. No que respeita à dimensão investigativa, dediquei especial atenção à “Organização de Ambientes Educativos Promotores da Educação em Ciências Naturais”, com vista a compreender como é que aprender ciências pode contribuir para a literacia científica das crianças tendo em vista o exercício de uma cidadania informada. Para tal as crianças envolveram-se ativamente no processo de construção do seu próprio conhecimento. Recorri a autores que me deram contributos para conceber, planear e fundamentar as práticas, quer do ponto de vista pedagógico quer do ponto de vista dos conteúdos curriculares. Para a recolha e tratamento da informação, atendi aos contextos onde o trabalho se realizou, bem como às observações dos diversos intervenientes na supervisão dos estágios, a planificações e reflexões semanais que fui elaborando e me permitiram concretizar uma análise imprescindível para as aprendizagens concretizadas e apresentadas no presente Relatório; REPORT OF SUPERVISED TEACHING PRACTICE IN PRE-SCHOOL EDUCATION AND THE 1ST CYCLE OF PRIMARY EDUCATION: ORGANIZATION OF EDUCATIONAL ENVIRONMENTS PROMOTERS OF EDUCATION IN NATURAL SCIENCES Abstract: The present report, elaborated in the scope of the Practice of Supervised Teaching in Pre-School and in the Primary Education, makes to known the learning processes inherent to the practice developed in context. In Pre-School Education, I created a training memorial that allowed me to reflect on the experiences I had live in the internship, making possible the acquisition of professional’s knowledges and learnings. In Primary School the internship also assumed a reflexive perspective which allowed me to learn the profession, but the dynamic of the work with the children allowed me to, equally, the realization of a project lined up with the action-research. The contexts where differentiated by their specificity, by the institutions geographic location, by the cultural situation and by the learning level where they were developed. The educational practice in Primary School occurred in the school EB/JI Galopim de Carvalho in Évora and the Pre-School practice occurred in Núcleo de Educação Infantil Colônia Z-11, in Brazil in the city of Florianopolis. In the matter of the investigative dimension, I dedicated special attention to the “Organization of Educative Environments Promoters of Natural Sciences Education”, to understand how learning sciences can contribute to scientifically literacy of the children, having in view the exercise of an informed citizenship. For this the children involved actively in the process of building their own knowledge. I resorted to authors who give me contributes to conceive, to plan and to fundament the practices, both from the pedagogical point of view and from the curricular contents point of view. To the collect and treat of data, I attend to the contexts where the work were realized, to the observations of the diverse actors in the internship supervision, to the daily plans and weekly reflections which I had been elaborating and which allowed me to concretize an indispensable analysis to the learnings achieve and presented in the present report.

Quality improvement of traditional dry fermented sausages based on innovative technological strategies

Dry fermented sausages are highly appreciated food specialties, mainly in Portugal and other southern European countries. Therefore, all research efforts aiming at improving the food quality and safety of traditional dry sausages are of interest, since they are likely to result in products with higher added value and quality standards most suited to the requirements and concerns of the modern consumers. Among those efforts, it may be highlighted the studies involving innovative processing parameters and technologies to overcome practical problems gathered in the meat industry, which are mostly associated with food quality and safety. Additionally, characterization of traditional dry sausages and rationalization of their processing are essential for further achievement of any official certification. Thus, this article attempts to point out some research lines of highest interest in meat science (and particularly to the broad variety of regional dry fermented sausages), towards to the valorisation of technological, nutritional and commercial features. In addition, it is here emphasized the importance for the continuous improvement of the quality and safety of meat products as a way to respond to the current concerns regarding its consumption and the general advices in reducing its daily intake.