Determination of Mn2+ ion by solution scanometry as a new, simple and inexpensive method

In this research, scanometry was used as a new, simple, fast and inexpensive method for a colorimetric determination of Mn2+ ion in water samples and thermocouple wire through the use of periodate reagent in an acidic medium. The results showed the oxidization of colorless Mn2+ ion by periodate and the formation of a purplish MnO4- ion. The system had a linear range of 1.0 to 70.0 µg mL-1 Mn2+ ion with a detection limit of 0.314 µg mL-1 and a relative standard deviation of 2.77% for G color value. This method has the capability to determine low levels of Mn2+ ion in thermocouple wire and water samples.

Surface Modification of Ceramics

Ceramics are widely used in industrial applications due to their advantageous thermal and mechanical stability. Corrosion of ceramics is a great problem resulting in significant costs. Coating is one method of reducing adversities of corrosion. There are several different thin film deposition processes available such as sol-gel, Physical and Chemical Vapour Deposition (PVD and CVD). One of the CVD processes, called Atomic Layer Deposition (ALD) stands out for its excellent controllability, accuracy and wide process capability. The most commonly mentioned disadvantage of this method is its slowness which is partly compensated by its capability of processing large areas at once. Several factors affect the ALD process. Such factors include temperature, the grade of precursors, pulse-purge times and flux of precursors as well as the substrate used. Wrongly chosen process factors may cause loss of self-limiting growth and thus, non-uniformities in the deposited film. Porous substrates require longer pulse times than flat surfaces. The goal of this thesis was to examine the effects of ALD films on surface properties of a porous ceramic material. The analyses applied were for permeability, bubble point pressure and isoelectric point. In addition, effects of the films on corrosion resistance of the substrate in aqueous environment were investigated. After being exposured to different corrosive media the ceramics and liquid samples collected were analysed both mechanically and chemically. Visual and contentual differences between the exposed and coated ceramics versus the untreated and uncoated ones were analysed by scanning electron microscope. Two ALD film materials, dialuminium trioxide and titanium dioxide were deposited on the ceramic substrate using different pulse times. The results of both film materials indicated that surface properties of the ceramic material can be modified to some extent by the ALD method. The effect of the titanium oxide film on the corrosion resistance of the ceramic samples was observed to be fairly small regardless of the pulse time.

Tulevaisuuden älykotiliiketoiminnan toimitusverkoston kartoitus

Tutkimus kartoitetaan tulevaisuuden älykotiliiketoiminnan palvelutuottaja-verkostoa. Lisäksi delphi-prosessia seuraten, pyritään kyselyn kautta vali-doimaan tulevaisuudesta tehty skenaario. Kysely tehtiin 42 yrityksen stra-tegiasta vastaavalle johtajalle. Selvitettäviä asioita olivat skenaarion liike-toiminnallinen kiinnostavuus, toteutumiskelpoisuus ja yrityksen siinä ha-luama rooli. Lisäksi selvitettiin mm. yritysten yhteistyökyvykkyyttä, innova-tiivisuutta, asiakassuuntautuneisuutta ja teknologinen valmiutta. Pääpaino oli selvittää verkoston muodostaja eli johtaja yritys. Kysely validoi kaikkien vastanneiden osalta tulevan skenaarion. Tulosten perusteella pystyttiin erottamaan 12 potentiaalista johtajaa. Nämä erottui-vat kyselyn kaikilla osa-alueilla parempina kuin muut yritykset. Potentiaali-set johtajat näkivät tulevaisuuden optimistisempana kuin muut ja lisäksi ne harjoittavat jo nyt liiketoimintaa, joka on lähellä kuvattua älykotipalveluver-kostoa. Tuloksia voidaan hyödyntää muodostettaessa palveluverkostoa uusille markkinoille. Kuvatun mallin toteutuminen vaatii kuitenkin julkisen sektorin palvelutoiminnan uudistusta, sillä se sisältää useita rinnakkaisia prosesseja julkisen terveydenhuollon kanssa.

A comparison of electricity spot prices simulation using ARMA-GARCH and mean-reverting models

The aim of this work is to compare two families of mathematical models for their respective capability to capture the statistical properties of real electricity spot market time series. The first model family is ARMA-GARCH models and the second model family is mean-reverting Ornstein-Uhlenbeck models. These two models have been applied to two price series of Nordic Nord Pool spot market for electricity namely to the System prices and to the DenmarkW prices. The parameters of both models were calibrated from the real time series. After carrying out simulation with optimal models from both families we conclude that neither ARMA-GARCH models, nor conventional mean-reverting Ornstein-Uhlenbeck models, even when calibrated optimally with real electricity spot market price or return series, capture the statistical characteristics of the real series. But in the case of less spiky behavior (System prices), the mean-reverting Ornstein-Uhlenbeck model could be seen to partially succeeded in this task.

Organisaatiorakenteiden vaikutuksia yritysten innovatiivisuuteen

Tämän työn tavoitteena oli selvittää, miten erilaiset organisaatiorakenteet vaikuttavat yritysten innovatiivisuuteen. Siinä käsitellään organisaatioiden rakenteiden jaottelua ja ominaisuuksia, ja käydään läpi innovatiivisuuteen tarvittavia resursseja ja siihen vaikuttavia ympäristön ominaisuuksia. Työn lopuksi tarkastellaan eri rakennemallien innovatiivisuutta edistäviä ja estäviä tekijöitä, ja tehdään yhteenveto innovatiivisuuden kannalta hyvistä ja huonoista rakenteista. Organisaatiorakenteet jaotellaan kuuteen malliin, mutta käytännössä niistä voidaan luoda yhdistelmiä kunkin organisaation tarpeen mukaan. Rakenteilla huomattiin olevan vaikutusta innovatiivisuuteen. Voidaan sanoa, että innovaatioiden kannalta parhaita ovat rakenteeltaan joustavat organisaatiot, joissa on matala hierarkiataso ja runsaasti kommunikaatiota. Esimerkkinä sellaisesta toimii erityisesti matriisirakenne. Huonosti innovaatiotoimintaa tukevia ovat toiminto- ja divisioonarakenteet, lähinnä joko reagoinnin hitauden tai asiantuntijuuden pinnallisuuden takia. Yrityksen tulee löytää tasapaino joustavuuden ja byrokratian välillä saavuttaakseen omalla toimialallaan tarpeellisen innovatiivisuuden. Sen tulee myös perehtyä tarpeisiinsa ja arvioida millaisia päämääriä todellisuudessa halutaan saavuttaa. Innovaatiotoiminnan kehittämisessä yrityksen johdon tulee ottaa huomioon nämä päämäärät ja kyseisen organisaation rakenne.

Uudistumiskyvyn ja innovatiivisuuden kehittäminen muutoshaasteena

Tämän Pro Gradun tavoitteena oli selvittää organisaation uudistumiskyvyn ja innovatiivisuuden kehittämisprosessiin liittyviä kriittisiä menestystekijöitä konsultin näkökulmasta. Työn toisena tavoitteena oli tuottaa karkea malli konsultointimetodista, jota erityisesti uudistumiskyvyn ja innovatiivisuuden kehittämisen parissa työskentelevät voisivat hyödyntää. Aluksi työssä tarkastellaan, mitä konsultointiprojektin menestyksen kannalta kriittisiä tekijöitä aiemmissa tutkimuksissa on havaittu. Tutkimuksen empiirisessä osassa kerättiin konsulttien näkemyksiä kriittisistä tekijöistä sekä siitä, kuinka uudistumiskyvyn ja innovatiivisuuden kehittämisen konsultointi poikkeaa muusta konsultoinnista. Tämän tutkimuksen tärkein tulos oli havainto, että selkeä ja toimijoiden kesken yhtenevä näkemys haasteista sekä kohdeorganisaation jäsenten laaja osallistaminen diagnoosi-, suunnittelu- ja toimeenpanovaiheen kaikissa toimissa edesauttavat hyvää ja kestävää lopputulosta. Konsultin keskeisin tehtävä on toimia jäsentelijänä, sparraajana, valmentajana sekä toteutuksen tukijana ja varmistajana.

Evolution of Bioaffinity Reagents by Phage Display

Antibodies are natural binding proteins produced in vertebrates as a response to invading pathogens and foreign substances. Because of their capability for tight and specific binding, antibodies have found use as binding reagents in research and diagnostics. Properties of cloned recombinant antibodies can be further improved by means of in vitro evolution, combining mutagenesis with subsequent phage display selection. It is also possible to isolate entirely new antibodies from vast naïve or synthetic antibody libraries by phage display. In this study, library techniques and phage display selection were applied in order to optimise binding scaffolds and antigen recognition of antibodies, and to evolve new and improved bioaffinity reagents. Antibody libraries were generated by random and targeted mutagenesis. Expression and stability were mainly optimised by the random methods whereas targeted randomisation of the binding site residues was used for optimising the binding properties. Trinucleotide mutagenesis allowed design of defined randomisation patterns for a synthetic antibody library. Improved clones were selected by phage display. Capture by a specific anti- DHPS antibody was exploited in the selection of improved phage display of DHPS. Efficient selection for stability was established by combining phage display selection with denaturation under reducing conditions. Broad-specific binding of a generic anti-sulfonamide antibody was improved by selection with one of the weakest binding sulfonamides. In addition, p9 based phage display was studied in affinity selection from the synthetic library. A TIM barrel protein DHPS was engineered for efficient phage display by combining cysteinereplacement with random mutagenesis. The resulting clone allows use of phage display in further engineering of DHPS and possibly use as an alternative-binding scaffold. An anti-TSH scFv fragment, cloned from a monoclonal antibody, was engineered for improved stability to better suite an immunoassay. The improved scFv tolerates 8 – 9 °C higher temperature than the parental scFv and should have sufficient stability to be used in an immunoanalyser with incubation at 36 °C. The anti-TSH scFv fragment was compared with the corresponding Fab fragment and the parental monoclonal antibody as a capturing reagent in a rapid 5-min immunoassay for TSH. The scFv fragment provided some benefits over the conventionally used Mab in anayte-binding capacity and assay kinetics. However, the recombinant Fab fragment, which had similar kinetics to the scFv, provided a more sensitive and reliable assay than the scFv. Another cloned scFv fragment was engineered in order to improve broad-specific recognition of sulfonamides. The improved antibody detects different sulfonamides at concentrations below the maximum residue limit (100 μg/kg in EU and USA) and allows simultaneous screening of different sulfonamide drug residues. Finally, a synthetic antibody library was constructed and new antibodies were generated and affinity matured entirely in vitro. These results illuminate the possibilities of phage display and antibody engineering for generation and optimisation of binding reagents in vitro and indicate the potential of recombinant antibodies as affinity reagents in immunoassays.

The Role of Integrins in Enterovirus Infections and in Metastasis of Cancer

Integrins are a family of transmembrane glycoproteins, composed of two different subunits (alpha and beta). Altered expression of integrins in tumor cells contributes to metastasis tendency by influencing on the cells‟ attachment to adjacent cells and their migration. Viral pathogens, including certain enteroviruses, use integrins as receptors. Enteroviruses have also been suggested to be involved in the etiopathogenesis of type 1 diabetes. The study focuses on the role of integrins in the pathogenesis of metastasis to cortical bone and on type 1 diabetes (T1D) and echovirus 1 infection. In the first part of the thesis, the role of different integrins in the initial attachment of MDA-MD-231 breast cancer cells to bovine cortical bone disks was studied. A close correlation between alpha2beta1 and alpha3beta1 integrin receptor expression and the capability of the tumor to attach to bone were observed. In the second part, a possible correlation between susceptibility to enterovirus infections in diabetic children and differences in enterovirus receptor genes, including certain integrins, was investigated. In parallel, virus-specific neutralizing antibodies and diabetic risk alleles were studied. In the diabetic group, an amino acid change was detected in the polio virus receptor and the neutralizing antibody titers against echovirus 30 were lower. However, to obtain statistically sustainable results, a larger number of individuals should be analyzed. Echovirus 1 (EV1) enters cells by attaching to the alpha2I domain of the alpha2beta1 integrin. In the third part EV1 was shown to attach to a chimeric receptor construct of the transferrin receptor and the alpha2I domain and to enter cells through clathrin-mediated endocytosis that is normally not used by the virus. The chimeric receptor was recycled to the plasma membrane, whereas the virus remained in intracellular vesicles. The virus replication cycle was initiated in these cells, suggesting that evolution pressure could possibly cause the virus to evolve to use a different entry mechanism. Moreover, a cDNA microarray analysis of host gene expression during EV1 replication showed that 0.53% of the total genes, including several immediate early genes, were differently expressed.

Electric propulsion system in a modern sports car

In this thesis an electric propulsion system is designed on a device level using Cadence ORCAD. The vehicle belongs to the Helsinki Metropolia University of Applied Sci-ences and it is to compete in the Automotive X-Prize competition held in the USA. In this thesis the electric propulsion system and related electric safety measures are designed. Also electro-magnetic compatibility and interferences present in the system are examined by examining the birth mechanisms and transmission paths of interference. Per device effects of interference and solutions to minimize them were examined and proposed. Suitability of permanent magnet synchronous machines for passenger vehicle use was examined by examining the torque production capability of the motor and the torque requirements of the vehicle. Also a short overview of history of electric vehicles is given.

Lappeenranta-malli alueellisen julkisen verkon toteutustapana

Työssä perehdytään tapoihin, joilla alueellinen julkinen verkko voidaan toteuttaa. Työn lähtökohtana on näkemys, että tietoyhteiskunnassa pääsy verkkoon on perusedellytys. Tällöin lähes kaikissa kodeissa tulisi olla mahdollisuus kytkeytyä ja olla jatkuvasti kytkeytyneenä tietoverkkoon. Lappeenranta-malli määrittelee tavan toteuttaa alueellisen julkisen verkon peruspalvelut. Mallin erityispiirteenä on mahdollisuus ilmoitusten esittämiseen verkon käyttäjille. Työssä arvioidaan Lappeenranta-mallin sopivuutta alueellisen julkisen verkon toteutustavaksi ja mitataan mallin suorituskykyä. Työn osana toteutetaan Lappeenranta-malliin kuuluva yhdysliikennepiste Lappeenrannan teknillisen yliopiston käyttöön.

The relationship between first-order capabilities and online innovations. A comparative case study on the publishing sector.

The objective of this thesis was to study the relationship between firstorder capabilities and online innovations. First-order capabilities can be divided into market and technology capabilities, and they play an important role in the production of innovations. The study was carried out in publishing industry, where many changes have taken place in the online environment during the last few years. In the empirical research, four companies were studied, two magazine publishers and two newspaper publishers. The analysis was done in two phases; first every case was analyzed alone and then the cases were compared in cross-case analysis. The most important finding was the positive impact of market capability to the production of online innovations. The study also increased understanding about the relationship between market and technology capabilities and online innovations in general.

Cost evaluation process for competitors' products

A company’s capability to map out its cost position compared to other market players is important for competitive decision making. One aspect of cost position is direct product cost that illustrates the cost efficiency of a company’s product designs. If a company can evaluate and compare its own and other market players’ direct product costs, it can implement better decisions in product development and management, manufacturing, sourcing, etc. The main objective of this thesis was to develop a cost evaluation process for competitors’ products. This objective includes a process description and an analysis tool for cost evaluations. Additionally, process implementation is discussed as well. The main result of this thesis was a process description consisting of a sixteen steps process and an Excel based analysis tool. Since literature was quite limited in this field, the solution proposal was combined from many different theoretical concepts. It includes influences from reverse engineering, product cost assessment, benchmarking and cost based decision making. This solution proposal will lead to more systematic and standardized cost position analyses and result in better cost transparency in decision making.

Suunnitelma ohjelmistotestauksen adaptiivisen referenssimallin arviointiin

The aim of this bachelor’s thesis is to make a plan for the assessment of the adaptive reference model of software testing, which is based on the ISO/IEC 29119 testing standard. The assessment is not carried out in the scope of this thesis, but later in the related MASTO project. The ISO/IEC 29119 testing standard and the ISO/IEC 15504 process assessment standard are explained and a literary review is done about problems in software testing practices. Based on this background information a plan for the assessment is made. The assessment plan assesses the reference model from two perspectives. First the capability of the testing process described by the reference model is assessed and then the practical usefulness of the model is assessed.

Hankintaosaaminen Päijät-Hämeen sosiaali- ja terveydenhuollon kuntayhtymässä

Pro gradu -tutkielman tarkoituksena on tutkia Päijät-Hämeen sosiaali- ja terveydenhuollon kuntayhtymän hankintojen roolia ja asemaa koko organisaatiossa ja sen muodostamia tavoitteita ja haasteita hankinta-asiantuntijuudelle. Tutkielman avulla pyritään selvittämään, millaisista elementeistä organisaation hankinta-asiantuntijuus muodostuu ja millaiset tekijät vaikuttavat organisaation hankinta-asiantuntijuustarpeisiin. Tutkielma ottaa kantaa hankintojen roolituksista kumpuaviin asiantuntijuuden kehittämistarpeisiin. Tutkielma on luonteeltaan kvantitatiivisia sekä kvalitatiivisia menetelmiä yhdistelevä case-tutkimus. Pyrkimyksenä on kartoittaa kuntayhtymälle ehdotelma hankinta-asiantuntijuuden kehittämisestä haastattelujen ja kyselyn avulla sekä kirjallisuuteen perehtyen. Teorian pohjalta pystyttiin muodostamaan hankintapäällikölle, hankintasihteereille sekä OTO -ostajille osaamisprofiilit, joihin peilattiin tämän hetkistä osaamistasoa. Tutkimustulokset osoittivat, että hankinta-asiantuntijuus case -organisaatiossa on tällä hetkellä hyvällä tasolla. Hankintaprofiilista riippumatta kehittämistä kaivattiin Liiketoimintastrategian, Suomen hankintalainsäädännön, Sopimusoikeuden sekä Hankintaprosessin suhteen.

Cultural aspects of globalizing university-industry knowledge interaction in China

Previous studies of the local involvement of multinational corporation (MNC) subsidiaries focus on host-country firms and local business partners such as suppliers and customers. The role of host-country universities in the same context of innovation networks is neglected. Furthermore, there are many organizational culture- and knowledge-related differences between universities and companies, and this is likely to pose additional challenges for successful collaboration. Early university-industry (U-I) studies have primarily been limited within a national boundary, being concerned with a single level of culture (i.e., at an organizational level) and one-way knowledge transfer from university to industry. Research on more dynamic knowledge interaction in multinational settings is lacking. This is particularly true in the business context of China. In today’s globalizing and rapidly changing organizations, addressing cultural differences and clashes is an everyday reality, and inter-cultural U-I collaboration is becoming a key asset for gaining global competitiveness. This study deals with Finnish MNC subsidiaries’ research collaboration with Chinese universities. It aims to explore the essence of such U-I collaboration and knowledge interaction, uncovering the deep functioning mechanisms of culture underlying effective collaborative knowledge creation and innovation. The study reviews critically different bodies of literature including knowledge management theories and studies, U-I collaboration and knowledge interaction, and cross-cultural research in terms of organizational knowledge generation and utilization. It adopts a case study strategy with qualitative research methods, and data is collected through in-depth interviews and participant observation. The study presents the following major findings: 1. In the light of a comprehensive analysis of U-I collaboration, an effective matching strategy is proposed, in the assumption that good alignment of knowledge interaction strategies and approaches with their corresponding knowledge type, capability development and research task may greatly enhance the effectiveness of cross-cultural U-I collaboration and knowledge interaction. 2. It is proposed that in the Chinese MNC context more dynamic types of knowledge interaction like knowledge co-creation should be of key concern particularly when dealing simultaneously with multi-disciplinary applied research of human factors and technologies. U-I knowledge interaction, otherwise, pays attention only to the study of one-way technology and knowledge transfer. 3. It is posited that the influence of culture on collaborative knowledge interaction can be studied in a valuable way when knowledge-related variables are simultaneously taken into account. A systematic analysis of the role of knowledge in cross-cultural knowledge interaction could best be approached from multi-aspects of knowledge including not only nature, characteristics and types of knowledge but also the process of knowledge (e.g., intensifications of knowledge interaction). 4. The study demonstrates the significant role of aspects of the host-country culture (e.g., Chinese guanxi) in U-I collaboration and knowledge interaction. This is evident, for instance, in issues related to interpersonal relationships and trust, true interest and the relatedness of the research, mutual commitment and learning, communication intensity and interaction, and awareness of cultural and knowledge-related differences between collaboration partners. Theoretical and practical implications of the findings are suggested and discussed.