From environmental concerns towards sustainable food provisioning. : Material flow and food consumption scenario studies on sustainability of agri-food systems

Agriculture is an economic activity that heavily relies on the availability of natural resources. Through its role in food production agriculture is a major factor affecting public welfare and health, and its indirect contribution to gross domestic product and employment is significant. Agriculture also contributes to numerous ecosystem services through management of rural areas. However, the environmental impact of agriculture is considerable and reaches far beyond the agroecosystems. The questions related to farming for food production are, thus, manifold and of great public concern. Improving environmental performance of agriculture and sustainability of food production, sustainabilizing food production, calls for application of wide range of expertise knowledge. This study falls within the field of agro-ecology, with interphases to food systems and sustainability research and exploits the methods typical of industrial ecology. The research in these fields extends from multidisciplinary to interdisciplinary and transdisciplinary, a holistic approach being the key tenet. The methods of industrial ecology have been applied extensively to explore the interaction between human economic activity and resource use. Specifically, the material flow approach (MFA) has established its position through application of systematic environmental and economic accounting statistics. However, very few studies have applied MFA specifically to agriculture. The MFA approach was used in this thesis in such a context in Finland. The focus of this study is the ecological sustainability of primary production. The aim was to explore the possibilities of assessing ecological sustainability of agriculture by using two different approaches. In the first approach the MFA-methods from industrial ecology were applied to agriculture, whereas the other is based on the food consumption scenarios. The two approaches were used in order to capture some of the impacts of dietary changes and of changes in production mode on the environment. The methods were applied at levels ranging from national to sector and local levels. Through the supply-demand approach, the viewpoint changed between that of food production to that of food consumption. The main data sources were official statistics complemented with published research results and expertise appraisals. MFA approach was used to define the system boundaries, to quantify the material flows and to construct eco-efficiency indicators for agriculture. The results were further elaborated for an input-output model that was used to analyse the food flux in Finland and to determine its relationship to the economy-wide physical and monetary flows. The methods based on food consumption scenarios were applied at regional and local level for assessing feasibility and environmental impacts of relocalising food production. The approach was also used for quantification and source allocation of greenhouse gas (GHG) emissions of primary production. GHG assessment provided, thus, a means of crosschecking the results obtained by using the two different approaches. MFA data as such or expressed as eco-efficiency indicators, are useful in describing the overall development. However, the data are not sufficiently detailed for identifying the hot spots of environmental sustainability. Eco-efficiency indicators should not be bluntly used in environmental assessment: the carrying capacity of the nature, the potential exhaustion of non-renewable natural resources and the possible rebound effect need also to be accounted for when striving towards improved eco-efficiency. The input-output model is suitable for nationwide economy analyses and it shows the distribution of monetary and material flows among the various sectors. Environmental impact can be captured only at a very general level in terms of total material requirement, gaseous emissions, energy consumption and agricultural land use. Improving environmental performance of food production requires more detailed and more local information. The approach based on food consumption scenarios can be applied at regional or local scales. Based on various diet options the method accounts for the feasibility of re-localising food production and environmental impacts of such re-localisation in terms of nutrient balances, gaseous emissions, agricultural energy consumption, agricultural land use and diversity of crop cultivation. The approach is applicable anywhere, but the calculation parameters need to be adjusted so as to comply with the specific circumstances. The food consumption scenario approach, thus, pays attention to the variability of production circumstances, and may provide some environmental information that is locally relevant. The approaches based on the input-output model and on food consumption scenarios represent small steps towards more holistic systemic thinking. However, neither one alone nor the two together provide sufficient information for sustainabilizing food production. Environmental performance of food production should be assessed together with the other criteria of sustainable food provisioning. This requires evaluation and integration of research results from many different disciplines in the context of a specified geographic area. Foodshed area that comprises both the rural hinterlands of food production and the population centres of food consumption is suggested to represent a suitable areal extent for such research. Finding a balance between the various aspects of sustainability is a matter of optimal trade-off. The balance cannot be universally determined, but the assessment methods and the actual measures depend on what the bottlenecks of sustainability are in the area concerned. These have to be agreed upon among the actors of the area

Supistamista, rationalisointia vai kehittämistä? : Valtiovarainministeriön järjestelyosaston järjestämän henkilöstökoulutus- ja tutkimustoiminnan rakentuminen osaksi valtionhallintoa vuosina 1943-1971

The study examines the personnel training and research activities carried out by the Organization and Methods Division of the Ministry of Finance and their becoming a part and parcel of the state administration in 1943-1971. The study is a combination of institutional and ideological historical research in recent history on adult education, using a constructionist approach. Material salient to the study comes from the files of the Organization and Methods Division in the National Archives, parliamentary documents, committee reports, and the magazines. The concentrated training and research activities arranged by the Organization and Methods Division, became a part and parcel of the state administration in the midst of controversial challenges and opportunities. They served to solve social problems which beset the state administration as well as contextual challenges besetting rationalization measures, and organizational challenges. The activities were also affected by a dependence on decision-makers, administrative units, and civil servants organizations, by different views on rationalization and the holistic nature of reforms, as well as by the formal theories that served as resources. It chose long-term projects which extended to the political decision-makers and administrative units turf, and which were intended to reform the structures of the state administration and to rationalize the practices of the administrative units. The crucial questions emerged in opposite pairs (a constitutional state vs. the ideology of an administratively governed state, a system of national boards vs. a system of government through ministries, efficiency of work vs. pleasantness of work, centralized vs. decentralized rationalization activities) which were not solvable problems but impossible questions with no ultimate answers. The aim and intent of the rationalization of the state administration (the reform of the central, provincial, and local governments) was to facilitate integrated management and to render a greater amount of work by approaching management procedures scientifically and by clarifying administrative instances and their respon-sibilities in regards to each other. The means resorted to were organizational studies and committee work. In the rationalization of office work and finance control, the idea was to effect savings in administrative costs and to pare down those costs as well as to rationalize and heighten those functions by developing the institution of work study practitioners in order to coordinate employer and employee relationships and benefits (the training of work study practitioners, work study, and a two-tier work study practitioner organization). A major part of the training meant teaching and implementing leadership skills in practice, which, in turn, meant that the learning environment was the genuine work community and efforts to change it. In office rationalization, the solution to regulate the relations between the employer and the employees was the co-existence of the technical and biological rationalization and the human resource administration and the accounting and planning systems at the turn of the 1960s and 1970s. The former were based on the school of scientific management and human relations, the latter on system thinking, which was a combination of the former two. In the rationalization of the state administration, efforts were made to find solutions to stabilize management ideologies and to arrange the relationships of administrative systems in administrative science - among other things, in the Hoover Committee and the Simon decision making theory, and, in the 1960s, in system thinking. Despite the development-related vocabulary, the practical work was advanced rationalization. It was said that the practical activities of both the state administration and the administrative units depended on professional managers who saw to production results and human relations. The pedagogic experts hired to develop training came up with a training system, based on the training-technological model where the training was made a function of its own. The State Training Center was established and the training office of the Organization and Methods Division became the leader and coordinator of personnel training.

Functional role of the Mso1p-Sec1p complex in membrane fusion regulation

Sec1/Munc18 (SM) protein family members are evolutionary conserved proteins. They perform an essential, albeit poorly understood function in SNARE complex formation in membrane fusion. In addition to the SNARE complex components, only a few SM protein binding proteins are known. Typically, their binding modes to SM proteins and their contribution to the membrane fusion regulation is poorly characterised. We identified Mso1p as a novel Sec1p interacting partner. It was shown that Mso1p and Sec1p interact at sites of polarised secretion and that this localisation is dependent on the Rab GTPase Sec4p and its GEF Sec2p. Using targeted mutagenesis and N- and C-terminal deletants, it was discovered that the interaction between an N-terminal peptide of Mso1p and the putative Syntaxin N-peptide binding area in Sec1p domain 1 is important for membrane fusion regulation. The yeast Syntaxin homologues Sso1p and Sso2p lack the N-terminal peptide. Our results show that in addition to binding to the putative N-peptide binding area in Sec1p, Mso1p can interact with Sso1p and Sso2p. This result suggests that Mso1p can mimic the N-peptide binding to facilitate membrane fusion. In addition to Mso1p, a novel role in membrane fusion regulation was revealed for the Sec1p C-terminal tail, which is missing in its mammalian homologues. Deletion of the Sec1p-tail results in temperature sensitive growth and reduced sporulation. Using in vivo and in vitro experiments, it was shown that the Sec1p-tail mediates SNARE complex binding and assembly. These results propose a regulatory role for the Sec1p-tail in SNARE complex formation. Furthermore, two novel interaction partners for Mso1p, the Rab GTPase Sec4p and plasma membrane phospholipids, were identified. The Sec4p link was identified using Bimolecular Fluorescence Complementation assays with Mso1p and the non-SNARE binding Sec1p(1-657). The assay revealed that Mso1p can target Sec1p(1-657) to sites of secretion. This effect is mediated via the Mso1p C-terminus, which previously has been genetically linked to Sec4p. These results and in vitro binding experiments suggest that Mso1p acts in cooperation with the GTP-bound form of Sec4p on vesicle-like structures prior to membrane fusion. Mso1p shares homology with the PIP2 binding domain of the mammalian Munc18 binding Mint proteins. It was shown both in vivo and in vitro that Mso1p is a phospholipid inserting protein and that this insertion is mediated by the conserved Mso1p amino terminus. In vivo, the Mso1p phospholipid binding is needed for sporulation and Mso1p-Sec1p localisation at the sites of secretion at the plasma membrane. The results reveal a novel layer of membrane fusion regulation in exocytosis and propose a coordinating role for Mso1p in connection with membrane lipids, Sec1p, Sec4p and SNARE complexes in this process.

Leuconostoc spoilage of refrigerated, packaged foods

Leuconostoc spp. are lactic acid bacteria (LAB) implicated in food spoilage, especially on refrigerated, modified atmosphere packaged (MAP) meats. The overall aim of this thesis was to learn more about Leuconostoc spp. as food spoilage organisms with a focus on commercial products where LAB spoilage is considered a problem and the main factor limiting shelf-life. Therefore, we aimed to identify Leuconostoc spp. involved in food spoilage, as well as to characterise the spoilage reactions they caused and their contamination sources during poultry meat processing. In addition, we examined the distribution of strains of Leuconostoc gasicomitatum in different food commodities. Finally, we analysed the genome content of L. gasicomitatum LMG 18811 with a special focus on metabolic pathways related to food spoilage. The findings show that Leuconostoc gelidum and L.gasicomitatum were responsible for the discoloration and off-odours developed in beef steaks. Together with Leuconostoc mesenteroides, these Leuconostoc spp., also cause spoilage of vegetable sausages. In contrast, we showed that Leuconostoc spp. are not important for the shelf-life or quality of non-marinated broiler products although, in marinated broiler fillet products, Leuconostoc spp., L.gasicomitatum in particular, are considered spoilage organisms. Furthermore, the findings of the contamination survey we carried out in a poultry processing plant indicated that spoilage Leuconostoc spp. are derived from the processing environment rather than from the broilers, and that air movement distributes psychrotrophic spoilage LAB, including leuconostocs, and has an important role in meat contamination during poultry processing. Pulsed-field gel electrophoresis (PFGE) based genotyping of L. gasicomitatum strains demonstrated that certain genotypes are common in various meat products. In contrast, genotypes associated with meat were not recovered in vegetable-based sources. This suggests that these two food categories either become contaminated with, or favour the growth of different genotypes. Furthermore, the results indicated that the meat processing environment contributes to L. gasicomitatum contamination as certain genotypes were repeatedly identified from products of the same processing plant. Finally, the sequenced and annotated genome of L.gasicomitatum LMG 18811 allowed us to identify the metabolic pathways and reactions resulting in food spoilage.

Identification of genetic susceptibility loci for migraine

Migraine is the common cause of chronic episodic headache, affecting 12%-15% of the Caucasian population (41 million Europeans and some half a million Finns), and causes considerable loss of quality of life to its sufferers, as well as being linked to increased risk for a wide range of conditions, from depression to stroke. Migraine is the 19th most severe disease in terms of disability-adjusted life years, and 9th among women. It is characterized by attacks of headache accompanied by sensitivity to external stimuli lasting 4-72 hours, and in a third of cases by neurological aura symptoms, such as loss of vision, speech or muscle function. The underlying pathophysiology, including what triggers migraine attacks and why they occur in the first place, is largely unknown. The aim of this study was to identify genetic factors associated with the hereditary susceptibility to migraine, in order to gain a better understanding of migraine mechanisms. In this thesis, we report the results of genetic linkage and association analyses on a Finnish migraine patient collection as well as migraineurs from Australia, Denmark, Germany, Iceland and the Netherlands. Altogether we studied genetic information of nearly 7,000 migraine patients and over 50,000 population-matched controls. We also developed a new migraine analysis method called the trait component analysis, which is based on individual patient responses instead of the clinical diagnosis. Using this method, we detected a number of new genetic loci for migraine, including on chromosome 17p13 (HLOD 4.65) and 10q22-q23 (female-specific HLOD 7.68) with significant evidence of linkage, along with five other loci (2p12, 8q12, 4q28-q31, 18q12-q22, and Xp22) detected with suggestive evidence of linkage. The 10q22-q23 locus was the first genetic finding in migraine to show linkage to the same locus and markers in multiple populations, with consistent detection in six different scans. Traditionally, ion channels have been thought to play a role in migraine susceptibility, but we were able to exclude any significant role for common variants in a candidate gene study of 155 ion transport genes. This was followed up by the first genome-wide association study in migraine, conducted on 2,748 migraine patients and 10,747 matched controls followed by a replication in 3,209 patients and 40,062 controls. In this study, we found interesting results with genome-wide significance, providing targets for future genetic and functional studies. Overall, we found several promising genetic loci for migraine providing a promising base for future studies in migraine.

Topeliaaninen usko : Kirjailija Sakari Topelius uskontokasvattajana

ABSTRACT The author Zacharias Topelius as a religious educator The present study concerns the author Zacharias Topelius (1818-98) as a religious educator. The study´s main questions are as follows: What is the theological and pedagogical content of Topelius books and how is his religious instruction linked with the history of his time. The primary sources are his educational books Naturens bok (The Book of the Nature, 1856), Boken om vårt land (The Book of our Land, 1875) and Evangelium för Barnen (Gospel for Children, 1893), as well as his storybooks Läsning för barn I-VIII (Reading for Children I-VIII, 1865-96). The dissertation concerns the his-tory of religious education. Its primary method is background-based systematic analy-sis. In Topelius children s books the view of God is characterised both as an omnipresent spirit and as Providence, who guides world history according to his plan. In addition to Lutheranism this view is also influenced by Nationalism and Romanticism. The theological content of the books emphasises instruction in Christian life that is natural to normative children s books. Topelius strongly expresses the importance of a personal relationship to God, an idealistic Christian view of one s fellow man and of one s own nation as well as the value of nature conservation. The books of Topelius were some of the first educational works on nature preservation in Finland. The didactic quality of Topelius children s books was high for 19th century Finland. Their main emphasis in terms of educational goals is on civilisation (Bildung), self-awareness, national solidarity and living idealism. The pedagogical argumentation is mostly based on theological, historical, social and rational reasoning. The primary principles in Topelius teaching are Christian nationalism, idealistic harmony and the agrarian bourgeois. Christian nationalism is the main element of Topelius religious education. He considers the fatherland as a God-given project and the taking care of it as a part of holy service. Idealistic harmony is seen as the comprehensive development of one s character in the sense of romantic idealism. The agrarian bourgeois principle combines the Finnish peasant tradition with the values of 19th century modern bourgeois culture. I have named Topelius vision of religious education the Christian national project of civilisation (Bildung). Its main theses are home, religion and fatherland. The author himself strongly believed in this vision and never questioned it despite its national chauvinism and theological inconsistency. The religious ideology represented in Topelius educational works and storybooks was popular among pedagogues during the whole era of the Finnish folk school. It fit per-fectly with the Christian national discourse stemming from 19th century ideological ten-dencies. Due to their appropriate content combined with their practical language and pedagogical methods, the books were popular both at school and in the home for a long period of time. Therefore the books of Topelius aptly symbolise the religious education of their time and manifest their author s pedagogical talent as a national religious educator and as a populariser of Christian nationalism. Topelius books have had a lasting influence on Finnish religiosity. Key words: Topelius, theology, religion, education, nationalism and national project

Ihmissalakuljetus ja ihmiskauppa Helsingin Sanomissa ja Dagens Nyheterissä

Human smuggling and trafficking in human beings are phenomena that are often represented as global and growing problems. Human smuggling means that a person is taken to a country illegally which means that smuggling does not exist without states. Trafficking in human beings by contrast means the exploitation of persons which makes it a human rights violation. The news coverage about both phenomena, especially about human trafficking, has grown rapidly during the last decade. However, there has not been research on the news coverage about phenomena in Finland and the news coverage on trafficking in human beings is little researched even in European countries. In this thesis I am comparing critically the newspaper content on the phenomena in Finland and in Sweden from the viewpoint of political and moral geography. Besides the contexts of the news, I paid attention to how identities in different scales, including the scale of the body, were represented in the news and how the boundaries between different identities were drawn in the news. As a methodology I used content analysis to classify the context of the news and discourse analysis to analyze how the different scales and boundaries between them were represented. The results address that in Finland especially the human smuggling is considered as a border issue and Finland´s location between East and West is emphasized, which points out that Finland´s location is a crucial part of the Finnish identity. In addition the linkages between human trafficking and prostitution are often debated in the news from different aspects. In Sweden meanwhile its´ political activeness in the fight against trafficking in human beings and international crime especially in the EU level are emphasized. Trafficking in human beings likewise prostitution according to Swedish law is seen as violence against women and the news are strongly against buying of sex as well. The states themselves, the state authorities and the EU are represented as active actors in both countries whereas international crime is represented as a threat and regions outside EU as chaotic. Additionally, illegal immigrants and the victims of trafficking are stigmatised. According to the results, the news coverage of both phenomena are used in constructing a more integrated national and European identity.

Luottamus koulussa : Tutkimus yhdeksännen luokan oppilaiden luottamus- ja epäluottamuskertomuksista ja niiden merkityksestä oppilaiden kouluarjessa

The aim of this study is to explore trust at school and its meaning for 9th grade students. The intent is to investigate students views about trust and mistrust in school relationships. Three research questions are posed: 1) what meanings do students give to their experiences of trust and mistrust at school and how do they evaluate connection of these experiences to their well-being and enjoyment in the classroom? 2) what and how important, is the teacher s role according to the students writings, and 3) what might the different pedagogical and administrative structures of schools reveal about trust and mistrust in a particular school culture? The data consists of 134 writings of 9th grade students (secondary school) from three schools in one of the biggest cities in Finland. The schools differ from others in terms of their pedagogical or structural backgrounds. The study is restricted to the micro-level of, disposition of Educational Sociology, focusing on trust in schools relationships. The theoretical framework of the study is trust, as a part of social capital; however trust is also approached from the sociological, the psychological and philosophical perspective. The methodological approach is narrative research concerning school practice . Analysis of narrative consist mostly content analysis, but also some elements of holistic-content reading, thematic reading and categorical content. The analysis found three main themes: 1) individual stories of trust, 2) the teachers role in making trust possible in the classroom, and 3) school as a community of trust. According to the study trust at school (1) is a complex phenomenon consisting of people s ability to work together and to recognize the demands that different situations present. Trust at school is often taken for granted. In the students experiences trust is strongly connected to friendship, and the teacher s ability to connect with students. Students experiences of mistrust stem from bulling, school violence, lack of respect as well as teachers lacking basic professional behavior. School relationships are important for some students as source of enjoyment, but some feel that it is difficult to evaluate the connection between trust and enjoyment. The study found that students trust of teachers (2) is linked to the teacher s professional role as a teacher, a caring human being open to dialogue. In other words, the students describe teachers abilities to create a sense of trust in terms of three expectations: the teacher was better, the same or worse than expected. Better than expected, means the teacher engender a high degree of trust and has excellent communication skills. Same as expected means the teacher comes across as familiar and secure, while lower than expected means the teacher creates no trust and has poor communication skills. Finally, it was shown that trust at school (3) should not only exist between some individuals, but between (all) members of the school community. In other words, according to the study there is some evidence that trust is strongly committed to school culture. Further, trust seems to depend on (school-) cultural background, values, beliefs, expectations, norms as well as staff behaviour. The basic elements of an optimum level of trust at school are favourable school structure and pedagogical background; however, good relationships between teachers and students as well as high professional skills are also needed. Trust at school is built by good communication, working together and getting to know each other.

Vegaaniäidit ja -lapset Suomessa Suosituksista käytäntöön

Suomalaisia vegaaniäitejä ja lapsia ei ole aiemmin tutkittu ravitsemustieteen näkökulmasta. Suomalaisilla suosituksissa (Valtion ravitsemusneuvottelukunta, Sosiaali- ja terveysministeriö) lasten ja äitien vegaaniruokavalioon suhtaudutaan varauksellisesti. Pohjois-Amerikassa mm. American Dietetic Association ja American Academy of Pediatrics sen sijaan pitävät vegaaniruokavaliota ravitsemuksellisesti riittävänä myös raskauden ja imetyksen aikana sekä pikkulapsilla. Suomalainen tutkimustieto on tarpeen mm. laadittaessa uusia suosituksia tulevaisuudessa. Tutkimukseen osallistui 14 perhettä. Vegaaniruokavaliota oli noudatettu 11 raskauden ja imetyksen ajan. Syntymästään saakka vegaanilapsia oli 13. Menetelmänä oli kolmeosainen kyselylomake, jonka tutkittavat palauttivat postitse. Raskaus- ja imetysaikaa tarkastellessani käytin vertailuaineistona niitä seitsemää raskautta, jotka eivät täyttäneet vegaaniruokavalion määritelmää, mutta joiden aikana oli syöty tavanomaista sekaruokavaliota kasvispainotteisemmin. Tutkimukseen osallistuneet olivat koulutettuja ja suurin osa asui kaupungissa. Lapset olivat iältään keskimäärin 1 ½-vuotiaita (vaihteluväli pariviikkoisesta 5-vuotiaaseen). Perheet olivat etsineet itse aktiivisesti tietoa ravitsemusasioista ja tietoa oli saatu myös Vegaaniliitosta. Virallinen terveydenhuolto ei ollut juurikaan pystynyt tarjoamaan tietoa: kahdeksan perhettä oli käynyt ravitsemusterapeutin vastaanotolla, mutta vain yksi ilmoitti saaneensa tietoa sitä kautta. Useimmiten perheen ruokavalio oli herättänyt hämmennystä ja epätietoisuutta terveydenhuollossa. Jotkut olivat kokeneet erittäin negatiivista suhtautumista, etenkin lääkärien taholta, mutta myös myönteisiä kokemuksia oli. Vegaaniäitien keskimääräinen painonnousu raskauden aikana oli normaali ja lapset olivat syntyneet normaalipainoisina. Lasten kasvu oli normaalia. Kaikki äidit olivat huolehtineet D- ja B12-saannista. Myös kaikki lapset saivat ko. vitamiinitäydennyksiä lukuun ottamatta yhtä epäselvää tapausta D-vitamiinin suhteen. Vegaaniäidit imettivät pitkään ja olivat lähempänä imetyssuosituksia kuin suomalaiset yleensä. Tämän tutkimuksen perusteella suomalaisissa vegaaniperheissä ollaan tietoisia D- ja B12-vitamiinien tärkeydestä ja ravitsemusasioista yleensä. Sellaisia ongelmia ei noussut esille, etteikö ruokavaliota voisi suositella

Resistance responses blocking systemic movement of Potato virus A in potato

Peruna kestää A-virusta estämällä sen leviämistä Peruna on maissin ohella maailman kolmanneksi tärkein ravintokasvi vehnän ja riisin jälkeen. Perunaa lisätään kasvullisesti mukuloita istuttamalla, jolloin virukset siirtyvät sairaiden siemenmukuloiden välityksellä kasvukaudesta toiseen. Virustauteja voi torjua ainoastaan terveen siemenperunan ja kestävien lajikkeiden avulla. Kestävyys perustuu usein siihen, että kasvi estää viruksen leviämisen tartuntakohdasta välttyäkseen virustaudilta. Tässä työssä tutkittiin kolmea perunan A-viruksen (PVA) liikkumista estävää kestävyysmekanismia perunassa. Lisäksi työn kokeelliseen osaan oleellisesti kuuluvaa virustartutusta varten kehitettiin uusi paranneltu versio geenipyssystä. Tämä itse rakennettu laite optimoitiin PVA:n tartuttamiseen mahdollisimman helposti ja pienin käyttökustannuksin. Tutkimuksen kohteena olleessa perunan risteytysjälkeläistössä oli PVA:ta kestäviä kasveja (ryhmä nnr), jotka estivät viruksen liikkumisen aiheuttamatta oireita tartutuskohdassa, sekä kasveja, joissa PVA aiheutti kuolioläikkinä näkyvän yliherkkyysvasteen (ryhmä HR). Molemmissa kestävyystyypeissä virus pystyi monistumaan ja leviämään solusta soluun paikallisesti, mutta liikkuminen muihin kasvinosiin nilan kautta estyi. Ryhmän nnr kasveissa PVA-tartunta ei aiheuttanut tilastollisesti merkitsevää muutosta useimpien geenien ilmenemiseen tartuntakohdassa. Ainoastaan geeniperhe, joka ilmentää tiettyä proteinaasi-inhibiittoria (PI), reagoi PVA:han 24 tuntia tartutuksesta. Kun tämän PVA:han reagoivan geeniperheen jäsenet hiljennettiin nnr- perunalinjoissa, ne muuttuivat alttiiksi PVA:lle ja virus levisi tartuntakohdasta muihin kasvinosiin. Tulos osoittaa, että PI on viruskestävyystekijä. Lisäksi muut tutkimuksessa saadut tulokset tukevat mahdollisuutta, että PI estää PVA:n P1-proteinaasin toimintaa. HR-linjoissa todettiin erilaisiin puolustusvasteisiin liittyvien PR-geenien aktivoitumista PVA-tartunnan seurauksena, mutta myös ilman sitä kasvien kasvettua mullassa noin neljä viikkoa. Sen sijaan solukkoviljelyssä tai vasta kaksi viikkoa mullassa kasvaneissa kasveissa vastaavaa ei vielä todettu. Tulos viittaa siihen, että HR-perunat reagoivat herkemmin ympäristöön ja/tai kasvin kehitysasteeseen laukaisten puolustusvasteita, jotka saattavat parantaa kestävyyttä taudinaiheuttajia vastaan. Kolmas tutkittu kestävyystyyppi havaittiin Pito-perunalajikkeessa. Se muistutti nnr-kestävyyttä siten, että myös siinä viruksen liikkuminen nilassa muihin kasvinosiin estyi. PVA:n todettiin pysähtyvän vasta lehtiruodin tyvelle muodostuvaan irtoamisvyöhykkeeseen, mitä havainnollistettiin käyttämällä muunnettua PVA-rotua, joka tuotti UV-valossa fluoresoivaa vihreää valoa. Tulos viittaa siihen, että virus ei pääse kulkemaan vyöhykkeeseen kuuluvan suojaavan kerroksen läpi, jollei sillä ole pääsyä nilaan. Tällainen kestävyys on tarpeen, jotta virus ei voi korvata nilakuljetusta solusta soluun leviämisellä. Tulokset tuovat uusia näkökulmia kasvien viruskestävyyteen ja auttavat selittämään viruksen nilakuljetuksen estymistä sekä solusta soluun leviämisen pysähtymistä kestävissä kasveissa.

Kolmas sektori maaseutukunnissa

Tämän tutkimuksen tarkoituksena on hahmottaa, millaisena toimijana kolmas sektori eli järjestöt nähdään maaseutumaisissa kunnissa nyt, kun kunta- ja palvelurakenne on muuttumassa ja väestö ikääntymässä. Tutkimuksen perusteella maaseutukunnat odottavat kolmannelta sektorilta ja järjestöiltä panosta kaikkein eniten sosiaalisten verkostojen, niitä vahvistavan ihmisten kanssakäymisen ja yhteisöllisyyden vahvistajana. Erilaisten tapahtumien, kulttuuri-, liikunta-, nuoriso-, harrastus- ja vapaa-ajan toiminnan järjestäminen, ympäristönhoito, perinteen ja historian tallentaminen sekä asukas- ja kylätoiminnan ylläpitäminen ovat maaseutukunnissa toimivien järjestöjen vahvaa toimintakenttää. Tällaisen perinteisen järjestötoiminnan myös taloudellisen merkityksen nähdään korostuvan väestön terveyden ja toimintakyvyn eteen tehtävän ennaltaehkäisevän työn tarpeen kasvaessa. Toinen tutkimuksessa vahvasti esille noussut kolmannen sektorin toiminnan vahvuus liittyy järjestöjen toiminnan ihmisläheisyyteen ja inhimillisyyteen. Kuntakoon kasvaessa ja palvelutuotannon järjestelmien monimutkaistuessa monet tutut arkiset tavat hoitaa asioita menevät uusiksi. Varsinkin ikääntyville nämä muutokset ovat suuria ja aiheuttavat inhimillistä hätää, pelkoja ja kasvavaa yksinäisyyttä. Järjestöissä tämä avun tarve nähdään usein läheltä, ja ne ryhtyvät toimimaan avun ja palvelujen järjestämiseksi. Järjestöjen koetaan toimivan inhimillisesti, lähellä ihmisiä, helposti lähestyttävänä, tutuin kasvoin, kuuntelevin korvin, todellisiin tarpeisiin vastaten. Ajankohtainen poliittinen puhe tuo kolmannen sektorin esille nimenomaan palvelujen tuottajana. Järjestöt ovat myös monissa maaseutukunnissa paikallisesti merkittäviä palvelujen tuottajia ja työllistäjiä. Tutkimuksessa tuli kuitenkin selvästi esille se, ettei kolmannen sektorin palveluntuottajaroolin vahvistumista nähdä varsinkaan harvaan asutun maaseudun pienissä kunnissa kovin realistisena. Järjestöjen roolin ja tehtävien kasvua palvelujen tuottajana jarruttaa erityisesti rahoituksen epävarmuus sekä järjestöväen ikääntyminen ja aktiivisten toimijoiden vähäinen määrä. Kolmas sektori nähtiin varsinkin maaseutukunnissa tehdyissä haastatteluissa vapaaehtoistyön paikkana. Tämä työn järjestämisen periaate kolmatta sektoria määrittävänä tekijänä tuli esille huomattavasti useammin kuin esimerkiksi se, tavoitellaanko toiminnalla voittoa vai ei. On helppo ymmärtää, että kolmas sektori pyritään hahmottamaan vapaaehtoistyön paikaksi, varsinkin kun palvelujen tuottamisen rahoitus on sekä kunnissa että järjestöissä muodostumassa entistä vaikeammaksi kysymykseksi. Siihen, tehdäänkö työ järjestöissä palkattuna työnä vai vapaaehtoistyönä, sisältyy myös yksi kolmatta sektoria koskevan poliittisen keskustelun sokeista pisteistä. Keskustelussa ei ole edetty vielä siihen asti, että puhuttaisiin niistä rajoista, mitä maaseudun yhdistyksiltä ja niissä vapaaehtoiselta pohjalta toimivilta ihmisiltä voidaan ylipäätään edellyttää. Tämän tutkimuksen perusteella voidaan todeta, että mikäli kolmannen sektorin odotetaan osallistuvan sellaisten palvelujen tuottamiseen, jotka edellyttävät sitoutumista, säännöllisyyttä ja pitkäjänteisyyttä, ei tällaisia tehtäviä voida edellyttää hoidettavaksi järjestöissä vapaaehtoisvoimin, ilman korvausta. Tällaisten tehtävien hoitamiseen tarvitaan palkattuja työntekijöitä myös järjestöissä. Tarkasteltaessa kolmannen sektorin roolin ja tehtävien muuttumista maaseutumaisissa kunnissa ja peilattaessa sitä kuntien kasvaviin haasteisiin palvelujen järjestäjänä huomio keskittyy väistämättä ikääntyvän väestön tuki-, hoiva- ja hoitopalvelujen kysynnän kasvuun. Kolmannen sektorin kannalta keskeinen kysymys on, kuka tuottaa ja rahoittaa tulevaisuudessa nuorisopalvelut, liikuntapalvelut, kulttuuripalvelut, vapaa-ajan palvelut, virkistys- ja harrastustoimintaa tai ne välttämättömät tuki- ja hoivapalvelut, joihin kunnilla ei ole varaa. Suomessa on sellaisia alueita ja sellaisia palveluja, joissa julkiset palvelut eivät riitä ja yritysmäiselle palvelutuotannolle ei ole edellytyksiä. Järjestöjen palvelutoimintaa syntyy usein juuri tällaisiin tilanteisiin ja sinne, missä taloudellista voittoa tavoittelevia yrityksiä ei ole, joko toiminnan matalan tuottavuuden, pitkien välimatkojen ja harvan asutuksen aiheuttamien korkeampien tuotantokustannusten tai asiakkaiden alhaisen maksukyvyn takia. Ongelmallista on, ettei näitä maaseudun olosuhteita ja palvelumarkkinoiden erityispiirteitä ole otettu huomioon tuotaessa ja sovellettaessa EU-lähtöistä valtiontuki- ja kilpailulainsäädäntöä kansalliseen lainsäädäntöömme. Tämän tutkimuksen perusteella voidaan todeta, että Suomessa on alueita ja palveluja, joissa kansalaisten perusoikeuksien ja yhdenvertaisuuden toteutuminen on kyseenalaista, jos näitä maaseudun olosuhdetekijöitä ei huomioida esimerkiksi valtiontukea koskevissa säädöksissä ja palvelujen tuottamiseen ei ohjata julkista tukea. Tutkimuksessa tarkastellaan niitä määritelmiä ja rajapintoja, jotka liittyvät yritystoimintaan ja järjestötoimintaan, elinkeinotoimintaan ja yleishyödylliseen toimintaan, yleishyödyllisiin ja julkisiin palveluihin, taloudellisiin ja ei-taloudellisiin palveluihin, kuntien lakisääteisiin ja ei-lakisääteisiin tehtäviin sekä vapaaehtoistyöhön ja palkattuun ammattityöhön. Tutkimuksessa kysytään, miksi palveluja tuottavien järjestöjen ja yritysten välisen kilpailuneutraliteetin tulkinnoissa ja tätä koskevissa säädöksissä ollaan oltu Suomessa niin tarkkoja ja miksi aihe on noussut niin keskeiseksi keskusteltaessa järjestöjen roolista palvelujen tuottajana. Maaseudun pitkien etäisyyksien ja pienten asiakasmäärien palvelumarkkinoilla kilpailua suurempi ongelma on pikemminkin palvelutuottajien vähäinen määrä tai se, ettei tuottajia ja kilpailua ole lainkaan. Aiheellista on myös kysyä, mistä yritykset ja järjestöt kilpailevat, onko niiden tuottamien palvelujen sisältö sama, onko asiakasryhmä sama tai miten palvelujen tuottamiseen liittyvät laajemmat yhteiskunnalliset ja sosiaaliset tekijät tulee ottaa huomioon todellista kilpailutilannetta arvioitaessa. Tutkimus vahvistaa tarvetta keskustella ja tehdä poliittiset linjaukset siitä, miten Suomessa suhtaudutaan sellaisiin organisaatioihin, joiden lähtökohtana on taloudellisen voiton tavoittelun asemesta yhteiskunnallisen ongelman ratkaiseminen tai tietyn väestöryhmän, kuten lasten, vanhusten tai vammaisten tarpeisiin vastaaminen. Euroopan parlamentin tätä yhteisötaloutta koskeva päätös vuodelta 2009 velvoittaa jäsenmaat ottamaan omassa lainsäädännössään huomioon tämäntyyppisen sosiaaliset ja taloudelliset näkökohdat yhdistävän toiminnan.

Beyond Words : The European Court of Justice and Legal Certainty in Multilingual EU Law

This study addresses the issue of multilingualism in EU law. More specifically, it explores the implications of multilingualism for conceptualising legal certainty, a central principle of law both in domestic and EU legal systems. The main question addressed is how multilingualism and legal certainty may be reconciled in the EU legal system. The study begins with a discussion on the role of translation in drafting EU legislation and its implications for interpreting EU law at the European Court of Justice (ECJ). Uncertainty regarding the meaning of multilingual EU law and the interrelationship between multilingualism and ECJ methods of interpretation are explored. This analysis leads to questioning the importance of linguistic-semantic methods of interpretation, especially the role of comparing language versions for clarifying meaning and the ordinary meaning thesis, and to placing emphasis on other, especially the teleological, purpose-oriented method of interpretation. As regards the principle of legal certainty, the starting-point is a two-dimensional concept consisting of both formal and substantive elements; of predictability and acceptability. Formal legal certainty implies that laws and adjudication, in particular, must be predictable. Substantive legal certainty is related to rational acceptability of judicial decision-making placing emphasis on its acceptability to the legal community in question. Contrary to predictability that one might intuitively relate to linguistic-semantic methods of interpretation, the study suggests a new conception of legal certainty where purpose, telos, and other dynamic methods of interpretation are of particular significance for meaning construction in multilingual EU law. Accordingly, the importance of purposive, teleological interpretation as the standard doctrine of interpretation in a multilingual legal system is highlighted. The focus on rational, substantive acceptability results in emphasising discourse among legal actors among the EU legal community and stressing the need to give reasons in favour of proposed meaning in accordance with dynamic methods of interpretation including considerations related to purposes, aims, objectives and consequences. In this context, the role of ideal discourse situations and communicative action taking the form of interaction among the EU legal community in an ongoing dialogue especially in the preliminary ruling procedure is brought into focus. In order for this dialogue to function, it requires that the ECJ gives persuasive, convincing and acceptable reasons in justifying its decisions. This necessitates transparency, sincerity, and dialogue with the relevant audience.

Generalizations and Models in Ecology : Lawlikeness, Invariance, Stability, and Robustness

The question at issue in this dissertation is the epistemic role played by ecological generalizations and models. I investigate and analyze such properties of generalizations as lawlikeness, invariance, and stability, and I ask which of these properties are relevant in the context of scientific explanations. I will claim that there are generalizable and reliable causal explanations in ecology by generalizations, which are invariant and stable. An invariant generalization continues to hold or be valid under a special change called an intervention that changes the value of its variables. Whether a generalization remains invariant during its interventions is the criterion that determines whether it is explanatory. A generalization can be invariant and explanatory regardless of its lawlike status. Stability deals with a generality that has to do with holding of a generalization in possible background conditions. The more stable a generalization, the less dependent it is on background conditions to remain true. Although it is invariance rather than stability of generalizations that furnishes us with explanatory generalizations, there is an important function that stability has in this context of explanations, namely, stability furnishes us with extrapolability and reliability of scientific explanations. I also discuss non-empirical investigations of models that I call robustness and sensitivity analyses. I call sensitivity analyses investigations in which one model is studied with regard to its stability conditions by making changes and variations to the values of the model s parameters. As a general definition of robustness analyses I propose investigations of variations in modeling assumptions of different models of the same phenomenon in which the focus is on whether they produce similar or convergent results or not. Robustness and sensitivity analyses are powerful tools for studying the conditions and assumptions where models break down and they are especially powerful in pointing out reasons as to why they do this. They show which conditions or assumptions the results of models depend on. Key words: ecology, generalizations, invariance, lawlikeness, philosophy of science, robustness, explanation, models, stability

Paimen vailla hiippakuntaa : Kenttäpiispan viran synty ja vaikutus sotilaspapiston asemaan Suomen puolustusvoimissa ja kirkossa vuosina 1939 - 1944

The main purpose of this research is to shed light on the factors that gave rise to the office of Field Bishop in the years 1939-1944. How did military bishophood affect the status of the head of military pastoral care and military clergy during these years? The main sources of my research are the collections in the Finnish National Archives, and I use a historical-qualitative method. The position of the military clergy was debated within both the Church and the Defence Forces before 1939. At that stage, Church law did not yet recognize the office of the leading military priest, the Field Dean. There had been a motion in 1932 to introduce the office of a military bishop, but the bishops' synod blocked it. The concept of Field Bishop appeared for the first time in 1927 in a Finnish military document, which dealt with pastoral care in the Polish military. The Field Dean in Finland had regularly proposed improvements to the salary of the military clergy before the Winter War. After the Winter War, arguments were made for strengthening the position of the military clergy: these arguments were based on the increased respect shown towards this clergy, especially due to their role in the care of the fallen, which had become their task during the war. Younger members of the military clergy in particular supported the demands to improve their position within the Church and the army. The creation of a Field Bishop was perceived as strengthening the whole military clergy, as the Field Bishop was envisioned as a bishop within the Church and a general within the Defence Forces. During that time the Field Dean was still without any military rank. The idea of a Field Bishop was recommended to Mannerheim in June 1940, after which the Defence Forces lent their support to the cause. The status of the military clergy, in Church law, made it to the agenda of the Church council in January 1941, thanks largely to the younger priests' group influence and Mannerheim's leverage. The bishops opposed the notion of a Field Bishop mostly on theological grounds but were ready to concede that the position the Field Dean in Church law required further defining. The creation of the office of Field Bishop was blocked in the Church law committee report issued close to the beginning of the Continuation War. The onset of that war, however, changed the course of events, as the President of the Republic appointed Field Dean Johannes Björklund as Field Bishop. Speculation has abounded about Mannerheim's role in the appointment, but the truth of the matter is not clear. The title of Field Bishop was used to put pressure on the Church, and, at the same time, Mannerheim could remain detached from the matter. Later, in September 1941, the Church council approved the use of the Field Bishop title to denote the head of military pastoral care in Church law, and Field Bishops were assigned some of the duties formerly pertaining to bishops. Despite all expectations and hopes, the new office of Field Bishop did not affect the status of the military clergy within the Defence Forces, as no ranks were established for them, and their salary did not improve. However the office of the Field Bishop within Army HQ was transformed from a bureau into a department in the summer of 1942. At the beginning of the Continuation War, the Field Bishop was criticized by certain military and Church clergy for favouring Russian Orthodox Christians in Eastern Karelia. Björklund agreed in principle with most of the Lutheran clergy on the necessity of Lutheranizing East Karelia but had to take into account the realities at Army HQ. As well, at the same time the majority of the younger clergy were serving in the army, and there was a lack of parish priests on the home front. Bishop Lehtonen had actually expressed the wish that more priests could have been released from the front to serve in local parishes. In his notes Lehtonen accused Björklund of trying to achieve the position of Field Bishop by all possible means. However, research has revealed a varied group of people behind the creation of the office of Field Bishop, including in particular younger clergy and the Defence Forces.

Kansainväliset sanktiot – kauniita sanoja vai harkittuja tekoja? Miksi kansainväliset sanktiot eivät toimi - esimerkkimaina Rhodesia, Etelä-Afrikka ja Zimbabwe.

Tutkin Pro Gradu – tutkielmassani kansainvälisen yhteisön toimeenpanemien sanktioiden ongelmallisuutta, niiden toimivuuden näkökulmasta. Aiempien tapausten valossa, sanktioiden suurimpana kärsijänä on usein nähty olevan siviiliväestö. Sanktioista huolimatta ne henkilöt ja ryhmät joiden katsotaan ollen vastuussa tilanteesta, ovat kuitenkin kyenneet jatkamaan toimintaansa. Sanktio-keskustelua onkin jakanut suhtautuminen sanktioiden hyödyllisyyteen, ennen kaikkea siihen miksi niitä käytetään kun ne eivät toimi. Pyrin työlläni osallistumaan kyseiseen keskusteluun kansainvälisten suhteiden tutkimuksen näkökulmasta. Aikaisemmin akateemista keskustelua on käyty pitkälti oikeudellisesta sekä taloudellisesta näkökulmasta. Työ on teoriasidonnainen tutkimus, jossa vertailevan tapaustutkimuksen keinoin haetaan teorioista tukea esimerkkitapausten käsittelyyn sekä johtopäätöksien tulkintaan. Tutkimuskysymystä lähestytään ensiksi aihepiirin teoreettisen sisällön esittelyllä, jonka jälkeen sitä sovelletaan kolmeen esimerkkitapaukseen. Esimerkkitapaukset ovat Rhodesia, Etelä-Afrikka ja Zimbabwe. Maat on valittu niiden sanktioihin johtaneiden olosuhteiden samankaltaisuuden vuoksi. Zimbabwe on lisäksi esimerkki sanktioissa ja niiden käytössä tapahtuneesta muutoksesta. Muutos on ollut siirtyminen kokonaisvaltaisista sanktioista kohdennettuihin sanktioihin. Teoreettisena viitekehyksenä työssä on toimijoiden rationaalinen toiminta, jonka lähtökohtana ovat heidät omat intressit. Kyseinen toiminta myötävaikuttaa osaltaan sanktioiden toimimattomuuteen, toimijoiden suhteuttaessa aina oman toimintansa muiden toimintaan. Sanktiokeskustelun sijoittumista tieteellisessä keskustelussa selvennän regiimiteorian avulla. Sanktioiden eri osapuolten toimintaa pyrin vuorostaan tuomaan esille rationaalisen valinnan teorian, eritoten peliteorian, avulla. Mainitun lisäksi tuon esille myös poliittisen päätöksenteon epävarmuuden, sekä sen vaikutukset osapuolten toimintamalleihin. Hypoteesina tutkimukselle on, että sanktioita lähettävien maiden keskinäisen yhteistyön heikkous sekä niiden omat kansalliset intressit vaikeuttavat sanktioiden toimintaa. Lähteinä työssä on käytetty julkisia lähteitä, jotka käsittelevät aihepiirin yleistä sekä teoreettista keskustelua. Esimerkkitapauksissa on käytetty myös julkisia lähteitä, sekä aikalaiskirjoituksia. Viimeksi mainitun taustalla on kahden esimerkkitapauksen, Rhodesian ja Etelä-Afrikan, historiallinen näkökulma. Kolmen esimerkkitapauksen kautta, tutkielmassani vahvistuu siinä esitetty hypoteesi. Tämän mukaan sanktioiden toimintaa vaikeuttavat sanktiota lähettävien maiden keskinäisen yhteistyön puute sekä niiden kansalliset intressit. Yhtä lailla sanktioitavat maat ovat sanktioprosessien hitaudesta johtuen kyenneet ennakoimaan ja varautumaan tuleviin muutoksiin. Esimerkkitapauksista Rhodesian ja Etelä-Afrikan kohdalla halutun muutoksen taustalla voidaan katsoa olleen pääasiallisesti muut tekijät kuin sanktiot. Zimbabwen osalta voidaan vuorostaan todeta, että siinä missä kokonaisvaltaiset sanktiot eivät toimineet Etelä-Afrikan ja Rhodesian tapauksissa halutulla tavalla, kohdennetut sanktiot ovat olleet yhtä lailla ongelmallisia. Esimerkkitapausten tuloksista ei voida vetää vedenpitäviä johtopäätöksiä joilla kyettäisiin arvioimaan tai ennustamaan sanktioiden toimivuutta kokonaisvaltaisemmin. Syynä on kansainvälisten sanktio-tapausten yksilöllisyys sekä sanktioiden käytön kiistanalaisuus kansainvälisessä politiikassa.