3D Analysis of the TCR/pMHCII Complex Formation in Monkeys Vaccinated with the First Peptide Inducing Sterilizing Immunity against Human Malaria

T-cell receptor gene rearrangements were studied in Aotus monkeys developing high antibody titers and sterilizing immunity against the Plasmodium falciparum malaria parasite upon vaccination with the modified synthetic peptide 24112, which was identified in the Merozoite Surface Protein 2 (MSP-2) and is known to bind to HLA-DR beta 1*0403 molecules with high capacity. Spectratyping analysis showed a preferential usage of V beta 12 and V beta 6 TCR gene families in 67% of HLA-DR beta 1*0403-like genotyped monkeys. Docking of peptide 24112 into the HLA-DR beta 1*0401-HA peptide-HA1.7TCR complex containing the VDJ rearrangements identified in fully protected monkeys showed a different structural signature compared to nonprotected monkeys. These striking results show the exquisite specificity of the TCR/pMHCII complex formation needed for inducing sterilizing immunity and provide important hints for a logical and rational methodology to develop multiepitopic, minimal subunit-based synthetic vaccines against infectious diseases, among them malaria.

Conceptual Rapprochement and the Analysis of the Historical, Normative and Jurisprudential Treatment of the Rural Workers, as Regards the Need for Special Protection

This article aims to present an approach to the issue of farm or rural zone workers, including a labour law study of agrarian legal decisions, so as to demonstrate their importance in respect to social, economic and cultural rights in Colombia. The study will serve to illustrate through the history, the applicable law and the jurisprudence, the different ways in which farmers have been treated from the time of the origin until the arrival of modern systems of industrialization. It calls into question the effectiveness of existing laws and the role of the courts, in spite of globalization, to maintain the minimum rights and guarantees of farm workers who are considered to be a vulnerable population. In conclusion, this study seeks to illustrate the current role of the Labor law and the National Health Service in the area of demonstrating of the existence or absence of mechanisms to protect workers in rural areas and the need to create some mechanisms that involve social justice given its prime importance in the Constitution of 1991.

Analysis of the employee’s labor agreement suspension in Colombia and some legislation references

Considering that the employment contract suspension responds to labor stability,one of the most important principles of labor law is important to study it because itsprincipal purpose is to maintain the link between de employer and the employeedespite the presence of adversity or other situations that would break up the relationshipin other fields. However, at the occurrence of any of the grounds of suspensionmay be presented some questions or voids that it will try to be answer in this paper.Consequently we shall refer first to the definition, purpose and characteristics of thesuspension. Subsequently, will be analyzed in detail every single ground of contractsuspension in Colombia. Then, will be studied the effects of the suspension andwe will refer to the resumption of work, and conclude with the comparative analysisof the figure in some Hispanic countries (Mexico, Paraguay and Spain).

An analysis of the anxiolytic effects of ethanol on consummatory successive negative contrast

The anxiolytic properties of ethanol (1 g/kg, 15% dose, i.p.) were studied in two experiments with rats involving incentive downshifts from a 32% to a 4% sucrose solution. In Experiment 1, alcohol administration before a downshift from 32% to 4% sucrose prevented the development of consummatory suppression (consummatory successive negative contrast, cSNC). In Experiment 2, ethanol prevented the attenuating effects of partial reinforcement (random sequence of 32% sucrose and nothing) on cSNC, causing a retardation of recovery from contrast. These effects of ethanol on cSNC are analogous to those described for the benzodiazepine anxiolytic chlordiazepoxide, suggesting that at least some of its anxiolytic effects are mediated by the same mechanisms. 

Analysis of the alveolar recruitment maneuvers applied at seven intensive care units

Introduction: During the past years, alveolar recruitment maneuvers (RM) have produced growing interest due to their beneficial potential in pulmonary protection, and have been introduced in clinical practice. Objective: To describe and analyze the knowledge of MR and its application at seven intensive care units in the city of Cali, Colombia. Methods and materials: Descriptive Cross-Sectional Study with an intentional sample of 64 professionals working in seven intensive care units and who apply MR. The self-completed survey was made up of thirteen questions, and the application period was two months. Results: Out of 64 professionals surveyed, 77.8% of them follow a protocol guide; 54.7% employes during RM the ideal Positive end-expiratory pressure (PEEP), which maintains a saturation > 90% and a PaO2 > 60 mmHg; 42.1% tolerates airway pressures between 35 and 50 cmH2O; 48.4% perform RM with a progressive increase of the PEEP and a low tidal volume. Conclusions: Regarding the knowledge related to RM, heterogeneity was found in the answers. There is currently no consensus about which is the most effective and secure way to implement an MR. This study can be the starting point to create awareness towards the revision of knowledge, capacities and abilities that are required to perform RM.

An analysis of the relationship between wages in the public and private sector in colombia : a panel data approach

This document examines the time-series properties of the wage differentials that arise between the public and private sector in Colombia during the sample period 1984 to 2005. We Find conflicting results in unit-root and stationary tests when looking at wage differentials at an aggregate level (such as for men, women or both). However, when we analyse wage differentials at higher levels of disaggregation, treat them jointly as a panel of data, and allow for the presence of potential cross section dependence, there is more supportive evidence for the view that wage differentials are stationary. This implies that although wage differentials do exist, they have not been consistently increasing (or decreasing) over time.

The persistent colombian conflict bubnational analysis of the duration of violence

The growing empirical literature on the analysis of civil war has recently included the study of conflict duration at the cross-country level. This paper presents, for the first time, a within-country analysis of the determinants of violence duration. I focus on the experience of the Colombian armed conflict. While the conflict has been active for about five decades, local violence ebbs and flows and areas experiencing continuous conflict coexist with places that have been able to resile and where violence is mostly absent. I examine a wide range of factors potentially associated with violence duration at the municipal level, including scale variables, geographical conditions, economic and social variables, institutions and state presence, inequality, government intervention, and victimization variables. I characterize a few variables robustly correlated with the persistence of localized conflict, both across specifications and using different econometric models of duration analysis.

An analysis of the Spanish public universities missions in efficiency terms. 'Un análisis de las misiones de las universidades públicas españolas en términos de eficiencia'.

Resumen basado en el de la publicación

First verbal productions of a bilingual child learning Basque and Spanish simultaneously : analysis of the noun phrase. 'Primeras producciones verbales de niños bilingües aprendiendo castellano y euskera simultáneamente : análisis de los sustantivos en la frase'.

Estudiar la evolución del lenguaje en niños bilingües que hablan euskera y castellano. Hipótesis: se encontrarán pocas marcas gramaticales en las frases. En euskera aparecerán ciertos sufijos de declinación; se espera encontrar el determinante 'A', marca de los casos locativo y posesivo y posiblemente algunos dativos. En castellano se espera no encontrar marcas gramaticales con preposiciones y artículos y sí en los casos locativo y posesivo. No se hallarán marcas formales y sí algunas construcciones lingüísticas por categorías ya que parece que los sistemas gramaticales funcionan de manera autónoma. Un niño en su período entre un año y once meses hasta dos años y tres meses, que se desenvuelve en euskera en el ambiente familiar y en castellano en su ambiente social. Se trata de un estudio descriptivo que está dividido en cuatro apartados: relato del ambiente familiar y social en el que se desenvuelve el niño. Diferencias entre el castellano y el euskera. Teorías que explican este tipo de problemas. Análisis de datos recogidos en las cintas. Las marcas gramaticales en castellano y euskera se clasifican en dos grupos: prefijos y unidades aisladas que preceden al nombre como artículos, pseudoartículos. Sufijos, incluyendo las marcas de casos, y calificativos. Grabaciones en vídeo sobre la producción en castellano y en euskera en situaciones familiares espontáneas. Cada sesión de grabación dura 30 minutos para cada idioma. Análisis de los datos recogidos en la grabación después de haber realizado la transcripción. En este caso, el artículo castellano no aparece inmediatamente. Los artículos indefinidos son más frecuentes que los definidos. Las formas masculinas predominan sobre las femeninas. Los artículos en castellano y los nombres en castellano y euskera son las unidades gramaticales más numerosas. Hay pocos ejemplos de sufijos locativos y posesivos. Aparecen pocas marcas de plural en los nombres. Se usan otras cuantificaciones como números, palabras 'otro', 'más'. A este nivel gramatical no hay fusión de marcas de los dos idiomas: no hay terminaciones 'A' en palabras castellanas usadas en contexto hispano y no hay artículos castellanos delante de nombres vascos usados en contexto vasco.

An analysis of the problems Magisterio Students of English have when reading texts in English.

Este estudio analiza el tipo de errores que los estudiantes españoles del primer año de magisterio cometen al leer y entender diferentes artículos de revistas y periódicos en inglés. Los resúmenes hechos por los estudiantes después de leer los textos son un buen instrumento para medir el tipo de problemas que ellos tienen. Los errores más frecuentes son los que tienen que ver con la gramática y con la cohesión del texto, más que con el léxico del artículo.

An analysis of the problems Magisterio Students of English have when reading texts in English.'Un análisis de los problemas que tienen los estudiantes de inglés de magisterio cuando leen textos en inglés'.

Se analiza el tipo de errores que cometen los estudiantes españoles de primer curso de Magisterio (Lengua Extranjera) cuando leen artículos de periódicos y revistas en inglés. Se demuestra que el análisis de los resúmenes hechos por los estudiantes después de leer el texto es un buen instrumento para detectar el tipo de problema que tienen en la lectura.

An analysis of the moderating effects of age in the acceptance of Learning Management Systems. 'Análisis del efecto moderador de la edad en la aceptación de sistemas e-learning LMS'.

Resumen basado en el de la publicación

Assessment and detection of peer-bullying through analysis of the group context

Resumen tomado de la publicaci??n

Meta-analysis of the effectiveness of school substance abuse prevention programs in Spain

Resumen tomado de la publicaci??n