Todos lo haremos mejor en el futuro : de la UCUA a la AGAE diez años de evaluación.

Resumen tomado de la publicación. Monográfico con el título: evaluación en educación

Geometría de los mecanismos articulados.

Realizado en la Facultad de Ciencias de Valladolid, por 7 profesores del centro, para las asignaturas de Máquinas Articuladas y Geometría Algebraica para las licenciaturas en Matemáticas y en Ciencias Físicas. Su objetivo era desarrollar máquinas que dibujen curvas concretas que se predeterminen, reproducir movimientos con ellas y extender el estudio a transformaciones no lineales. Se han preparado manuales de problemas, un seminario y la elaboración de un paquete integrado de software para establecer las simulaciones oportunas. Se pretende la conexión con el Programa de Doctorado en Matemáticas, el cual ha obtenido la Mención de Calidad de la Agencia Nacional. Se han elaborado los siguientes materiales: máquinas, problemas de álgebra lineal (ISBN 84-8448-303-7), problemas de geometría afín (ISBN 84-8448-302-9) y textos del Seminario de Matemáticas. Resulta evidente la consecución de los objetivos propuestos y se hace mención del interés por completar este estudio y ampliar el contacto con el profesorado de Enseñanza Secundaria.

El poder de la ANECA : evaluar a la universidad y a sus profesores.

Resumen tomado de la revista

El valor de la inclusi??n educativa de j??venes con discapacidad intelectual en las instituciones de educaci??n superior : el Programa Promentor

Resumen tomado de la publicaci??n

Propuesta de evaluación de la actividad docente universitaria en entornos virtuales de aprendizaje.

Resumen basado en el de la publicación

Motivazzioni e soddisfazione di genere nel digital-learning : un studio di caso. 'Motivaciones y satisfaciones generizadas en el digital-learning : un estudio de caso'.

Monográfico con el título: 'Sociedad de la Información, lenguas minoritarias y educación en bilingüismo'. Resumen basado en el de la publicación

El Proceso de Bolonia : ¿ha propiciado una mayor incorporación de la responsabilidad social en los grados relacionados con la Economía y la Empresa?

Monográfico con el título: 'Competencias docentes en la Educación Superior'. Resumen basado en el de la publicación

Avaluaci?? de la qualitat docent i promoci?? del professorat : legislaci?? universit??ria espanyola : modificaci?? de la Llei Org??nica d'Universitats

Resumen basado en el de la publicaci??n

Proyecto Cl@ssi 2.0 : el primer paso hacia la escuela 2.0

Resumen basado en el de la publicaci??n

Indicadores de calidad de las publicaciones cient??ficas en el ??rea de Ciencias Sociales en Espa??a : un an??lisis comparativo entre agencias evaluadoras

Resumen basado en el de la publicaci??n

La ampliación de la frontera petrolera sobre los territorios campesinos en Colombia: conflictos y resistencias

En Colombia se expresa a cabalidad la tendencia mundial: se están ampliando las fronteras petroleras, pues las reservas de crudo convencional han disminuido. La expansión de la exploración y de la explotación hidrocarburífera en el país se expresa hoy en el cubrimiento de casi toda la geografía nacional con bloques petroleros y en un hecho sobresaliente: la incursión de esta industria en las altas montañas (cordillera de Los Andes), en donde hasta épocas recientes se había privilegiado la producción campesina. Así se observa, por ejemplo, en el altiplano cundiboyacense, donde ya existen bloques para la búsqueda de crudos no convencionales. Los artífices de esa situación son los últimos dos gobiernos, que en las conocidas y periódicas rondas petroleras han subastado bloques petroleros y han impulsado, con la conducción de la Agencia Nacional de Hidrocarburos, ANH, una agresiva campaña de adjudicación de los mismos. Comunidades con fuerte arraigo campesino, ajenas a esta actividad, reaccionan con solo notar la presencia de las empresas en su territorio, por los graves impactos que comienzan a tener desde que empieza a crearse el ambiente exploratorio.

Coupling Virus-Induced Gene Silencing to Exogenous Green Fluorescence Protein Expression Provides a Highly Efficient System for Functional Genomics in Arabidopsis and across All Stages of Tomato Fruit Development

Since the advent of the postgenomic era, efforts have focused on the development of rapid strategies for annotating plant genes of unknown function. Given its simplicity and rapidity, virus-induced gene silencing (VIGS) has become one of the preeminent approaches for functional analyses. However, several problems remain intrinsic to the use of such a strategy in the study of both metabolic and developmental processes. The most prominent of these is the commonly observed phenomenon of ""sectoring"" the tissue regions that are not effectively targeted by VIGS. To better discriminate these sectors, an effective marker system displaying minimal secondary effects is a prerequisite. Utilizing a VIGS system based on the tobacco rattle virus vector, we here studied the effect of silencing the endogenous phytoene desaturase gene (pds) and the expression and subsequent silencing of the exogenous green fluorescence protein (gfp) on the metabolism of Arabidopsis (Arabidopsis thaliana) leaves and tomato (Solanum lycopersicum) fruits. In leaves, we observed dramatic effects on primary carbon and pigment metabolism associated with the photobleached phenotype following the silencing of the endogenous pds gene. However, relatively few pleiotropic effects on carbon metabolism were observed in tomato fruits when pds expression was inhibited. VIGS coupled to gfp constitutive expression revealed no significant metabolic alterations after triggering of silencing in Arabidopsis leaves and a mild effect in mature green tomato fruits. By contrast, a wider impact on metabolism was observed in ripe fruits. Silencing experiments with an endogenous target gene of interest clearly demonstrated the feasibility of cosilencing in this system; however, carefully constructed control experiments are a prerequisite to prevent erroneous interpretation.

Genetic dissection of vitamin E biosynthesis in tomato

Vegetables are critical for human health as they are a source of multiple vitamins including vitamin E (VTE). In plants, the synthesis of VTE compounds, tocopherol and tocotrienol, derives from precursors of the shikimate and methylerythritol phosphate pathways. Quantitative trait loci (QTL) for alpha-tocopherol content in ripe fruit have previously been determined in an Solanum pennellii tomato introgression line population. In this work, variations of tocopherol isoforms (alpha, beta, gamma, and delta) in ripe fruits of these lines were studied. In parallel all tomato genes structurally associated with VTE biosynthesis were identified and mapped. Previously identified VTE QTL on chromosomes 6 and 9 were confirmed whilst novel ones were identified on chromosomes 7 and 8. Integrated analysis at the metabolic, genetic and genomic levels allowed us to propose 16 candidate loci putatively affecting tocopherol content in tomato. A comparative analysis revealed polymorphisms at nucleotide and amino acid levels between Solanum lycopersicum and S. pennellii candidate alleles. Moreover, evolutionary analyses showed the presence of codons evolving under both neutral and positive selection, which may explain the phenotypic differences between species. These data represent an important step in understanding the genetic determinants of VTE natural variation in tomato fruit and as such in the ability to improve the content of this important nutriceutical.

Genomic Analysis of Wild Tomato Introgressions Determining Metabolism- and Yield-Associated Traits

With the aim of determining the genetic basis of metabolic regulation in tomato fruit, we constructed a detailed physical map of genomic regions spanning previously described metabolic quantitative trait loci of a Solanum pennellii introgression line population. Two genomic libraries from S. pennellii were screened with 104 colocated markers from five selected genomic regions, and a total of 614 bacterial artificial chromosome (BAC)/cosmids were identified as seed clones. Integration of sequence data with the genetic and physical maps of Solanum lycopersicum facilitated the anchoring of 374 of these BAC/cosmid clones. The analysis of this information resulted in a genome-wide map of a nondomesticated plant species and covers 10% of the physical distance of the selected regions corresponding to approximately 1% of the wild tomato genome. Comparative analyses revealed that S. pennellii and domesticated tomato genomes can be considered as largely colinear. A total of 1,238,705 bp from both BAC/cosmid ends and nine large insert clones were sequenced, annotated, and functionally categorized. The sequence data allowed the evaluation of the level of polymorphism between the wild and cultivated tomato species. An exhaustive microsynteny analysis allowed us to estimate the divergence date of S. pennellii and S. lycopersicum at 2.7 million years ago. The combined results serve as a reference for comparative studies both at the macrosyntenic and microsyntenic levels. They also provide a valuable tool for fine-mapping of quantitative trait loci in tomato. Furthermore, they will contribute to a deeper understanding of the regulatory factors underpinning metabolism and hence defining crop chemical composition.

A DNA Vaccine Encoding the Enterohemorragic Escherichia coli Shiga-Like Toxin 2 A(2) and B Subunits Confers Protective Immunity to Shiga Toxin Challenge in the Murine Model

Production of verocytotoxin or Shiga-like toxin (Stx), particularly Stx2, is the basis of hemolytic uremic syndrome, a frequently lethal outcome for subjects infected with Stx2-producing enterohemorrhagic Escherichia coli (EHEC) strains. The toxin is formed by a single A subunit, which promotes protein synthesis inhibition in eukaryotic cells, and five B subunits, which bind to globotriaosylceramide at the surface of host cells. Host enzymes cleave the A subunit into the A(1) peptide, endowed with N-glycosidase activity to the 28S rRNA, and the A(2) peptide, which confers stability to the B pentamer. We report the construction of a DNA vaccine (pStx2 Delta AB) that expresses a nontoxic Stx2 mutated form consisting of the last 32 amino acids of the A(2) sequence and the complete B subunit as two nonfused polypeptides. Immunization trials carried out with the DNA vaccine in BALB/c mice, alone or in combination with another DNA vaccine encoding granulocyte-macrophage colony-stimulating factor, resulted in systemic Stx-specific antibody responses targeting both A and B subunits of the native Stx2. Moreover, anti-Stx2 antibodies raised in mice immunized with pStx2 Delta AB showed toxin neutralization activity in vitro and, more importantly, conferred partial protection to Stx2 challenge in vivo. The present vector represents the second DNA vaccine so far reported to induce protective immunity to Stx2 and may contribute, either alone or in combination with other procedures, to the development of prophylactic or therapeutic interventions aiming to ameliorate EHEC infection-associated sequelae.