Genetic dissection of vitamin E biosynthesis in tomato
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
20/10/2012
20/10/2012
2011
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Resumo |
Vegetables are critical for human health as they are a source of multiple vitamins including vitamin E (VTE). In plants, the synthesis of VTE compounds, tocopherol and tocotrienol, derives from precursors of the shikimate and methylerythritol phosphate pathways. Quantitative trait loci (QTL) for alpha-tocopherol content in ripe fruit have previously been determined in an Solanum pennellii tomato introgression line population. In this work, variations of tocopherol isoforms (alpha, beta, gamma, and delta) in ripe fruits of these lines were studied. In parallel all tomato genes structurally associated with VTE biosynthesis were identified and mapped. Previously identified VTE QTL on chromosomes 6 and 9 were confirmed whilst novel ones were identified on chromosomes 7 and 8. Integrated analysis at the metabolic, genetic and genomic levels allowed us to propose 16 candidate loci putatively affecting tocopherol content in tomato. A comparative analysis revealed polymorphisms at nucleotide and amino acid levels between Solanum lycopersicum and S. pennellii candidate alleles. Moreover, evolutionary analyses showed the presence of codons evolving under both neutral and positive selection, which may explain the phenotypic differences between species. These data represent an important step in understanding the genetic determinants of VTE natural variation in tomato fruit and as such in the ability to improve the content of this important nutriceutical. FAPESP, CNPq, and USP (Brazil) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) USP Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Max Planck Society (Germany) Max Planck Society (Germany) INTA, CONICET, and ANPCyT (Argentina) Consejo Nacional de Investigaciones Científicas y Técnicas de Argentina (CONICET) Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT) EU[FOOD-CT-2006-016214] EU CAPES (Brazil) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) FAPESP (Brazil) ANPCyT (Argentina) Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT) |
Identificador |
JOURNAL OF EXPERIMENTAL BOTANY, v.62, n.11, p.3781-3798, 2011 0022-0957 http://producao.usp.br/handle/BDPI/27638 10.1093/jxb/err055 |
Idioma(s) |
eng |
Publicador |
OXFORD UNIV PRESS |
Relação |
Journal of Experimental Botany |
Direitos |
closedAccess Copyright OXFORD UNIV PRESS |
Palavras-Chave | #Fruit metabolism #Solanum pennellii #tocopherol #tomato #vitamin E #AROMATIC-AMINO-ACIDS #3-DEOXY-D-ARABINO-HEPTULOSONATE 7-PHOSPHATE SYNTHASE #GERANYL DIPHOSPHATE SYNTHASE #MULTIPLE SEQUENCE ALIGNMENT #ARABIDOPSIS-THALIANA #SUBCELLULAR COMPARTMENTATION #TOCOPHEROL BIOSYNTHESIS #ISOPRENOID BIOSYNTHESIS #TRANSGENIC TOBACCO #SHIKIMATE PATHWAY #Plant Sciences |
Tipo |
article original article publishedVersion |