Otimização e validação de método empregando quechers modificado e lc-esi-ms/ms para determinação de agrotóxicos em cebola

An evaluation of the pesticides extraction from onion using a modern sample preparation method (QuEChERS) and determination by liquid chromatography tandem mass spectrometry was carried out. All the calibration curves showed r>0.99. The recoveries ranged between 61.8 and 120.0% with relative standard deviation lower than 20% for all compounds. Due to the occurrence of matrix effect, the quantification was performed using matrix-matched calibration. The limits of quantification of the method were between 0.0005 and 0.05 mg kg-1. The method shows the advantages of not require the clean-up step and consume low volume of organic solvents, decreasing time, costs and residues.

Otimização das condições de extração de saponinas em Ampelozizyphus amazonicus usando planejamento experimental e metodologia de superfície de resposta

This works describes the use of experimental design and surface response methodology for optimization of saponin extraction from Ampelozizyphus amazonicus. For this purpose, a method employing extraction based on maceration assisted by ultrasound technique was utilized. The following factors were studied: extraction length of time and solvent composition. The total saponin was determined by using a gravimetric method and the results expressed by their relative proportion to total crude extract. For the specific condition, 60% hydro-alcoholic solution and 18 minutes extraction length of time has shown the best results. This method can be useful for extraction of substances with biological importance

Isolamento do tungstênio da volframita da mina de Igarapé Manteiga (Rondônia - Brasil) por lixiviação ácida

We report results of the efficiency of tungsten extraction from wolframite concentrate (containing 61.5 wt % WO3) from the Igarapé Manteiga mine (state of Rondônia, Brazil) through acid leaching with strong mineral acids at 100 ºC and 400 rpm for 2-4 h. HCl yielded insoluble matter containing the highest WO3 content (90 wt %). This solid was dissolved in concentrated NH3(aq) at 25 ºC and the insoluble matter filtrated. The filtrate was slowly evaporated. 70 wt % of the tungsten present in the starting concentrate material was recovered as ammonium paratungstate (APT).

Inovação & propriedade industrial & indústria química

ABSTRACT The main aim of this paper was to contribute to reflections in Brazil on the need to transfer knowledge held at universities and R&D institutions over to companies, i.e. to transfer scientific knowledge of chemistry to technology. It discusses how the competitiveness of countries is increasingly dependent on their technological capacity. The chemicals industry is a fundamental driver of social, environmental, economic and industrial indicators of sustainable development. In Brazil, the chemicals industry's deficit has grown over the last three decades. Patents are important sources of information because patent documents contain 75% of all technological information available. The National Institute of Industrial Property in Brazil has created a Technology Observatory with the purpose of identifying and analyzing technological information contained in patent documents within the ambit of partnerships with government entities or business associations, in order to support their technology-related decision-making processes. The paper gives examples of ethanol and biotechnology patent documents, including pharmaceuticals, of which there are very few in Brazil. However, a few of the patent applications identified are filed in Brazil, giving the country the opportunity to transform this scientific knowledge into technology by means of partnership agreements with companies. Finally, the paper presents information on the patent applications filed by the world's leading chemicals companies as measured by their revenues, and the respective numbers of patent applications in the last five years in organic chemistry and polymers, sectors in which Brazil is currently dependent on imports for over 50% of its needs. The patent assignees in these sectors in Brazil are also identified, and the paper concludes that Brazil needs to invest in the development of professionals, providing clearly-defined career paths in technology innovation teams at R&D institutions, and to foster more initiatives such as the creation of a new research and innovation entity, EMBRAPII, since investing in science and technology is a prerequisite for knowledge production, industrial property, economic development and, consequently, the competitiveness of the country.

Detection and partial characterization of an isolate of Groundnut ringspot virus in Solanum sessiliflorum

The cubiu (Solanum sessiliflorum) fruit, originating in the Amazon basin, is commonly used in that region for food, medicine, and cosmetics. In an experimental culture of cubiu, in order to evaluate its adaptation to conditions in the Northern region of the state of Rio de Janeiro, it was observed plants with mosaic symptoms. A cubiu plant was collected and analyzed to identify the etiological agent. After mechanical passage through a local lesion host, a host range test was performed. The virus induced chlorotic local lesions in Chenopodium quinoa, necrotic local lesions in Gomphrena globosa, mosaic in S. sessiliflorum, leaf and stem necrosis in tomato (Lycopersicon esculentum) 'Rutgers', mosaic and leaf distortion in Datura stramonium and Physalis floridana, and necrotic local lesions followed by systemic necrosis and plant death in four Nicotiana species. Electron microscopic observations of ultra thin sections from infected cubiu leaves showed the presence of spheroidal, membrane-bound particles typical of tospovirus species. Analysis of the nucleocapsid protein from concentrated virus particles indicated the presence of a 28 kDa protein. RT-PCR was performed after total RNA extraction from infected IPA-6 tomato leaves. A fragment of approximately 0,8 kbp corresponding to the N gene was amplified, cloned and sequenced. The N protein from the cubiu isolate was 95% homologous to the Groundnut ringspot virus (GRSV) protein, and no more than 85% homologous to those from Zucchini lethal chlorosis virus (ZLCV) and Chrysanthemun stem necrosis virus (CSNV), Tomato spotted wilt virus (TSWV), and Tomato chlorotic spot virus (TCSV). This is the first report of the occurrence of GRSV (or any other plant virus) in cubiu.

Simulating the performance of paper machine control

The topic of this thesis is the simulation of a combination of several control and data assimilation methods, meant to be used for controlling the quality of paper in a paper machine. Paper making is a very complex process and the information obtained from the web is sparse. A paper web scanner can only measure a zig zag path on the web. An assimilation method is needed to process estimates for Machine Direction (MD) and Cross Direction (CD) profiles of the web. Quality control is based on these measurements. There is an increasing need for intelligent methods to assist in data assimilation. The target of this thesis is to study how such intelligent assimilation methods are affecting paper web quality. This work is based on a paper web simulator, which has been developed in the TEKES funded MASI NoTes project. The simulator is a valuable tool in comparing different assimilation methods. The thesis contains the comparison of four different assimilation methods. These data assimilation methods are a first order Bayesian model estimator, an ARMA model based on a higher order Bayesian estimator, a Fourier transform based Kalman filter estimator and a simple block estimator. The last one can be considered to be close to current operational methods. From these methods Bayesian, ARMA and Kalman all seem to have advantages over the commercial one. The Kalman and ARMA estimators seems to be best in overall performance.

KILPAILUETUA OSAAMISTA JOHTAMALLA - Tapaustutkimus innovatiivisessa teknologia-alan yrityksessä

Osaamisella on mahdollisuus luoda organisaatiolle kilpailuetua. Henkilöstön osaamisen johtaminen on yksi keskeinen tehtävä menestyvässä organisaatiossa. Osaamisen johtamisen tärkeimpänä tavoitteena on huolehtia strategialähtöisesti osaamisen kehittämisestä organisaation tehtävien suorittamiseksi. Tämän kvalitatiivisen case-tutkimuksen tavoitteena oli selvittää kohdeyrityksen osaamisen johtamisen nykytilaa ja kehittämismahdollisuuksia. Tutkimuksen teoreettisessa osassa esitellään henkilöstöjohtamisen perusteita, oppimista ja osaamista sekä osaamisen johtamista. Tutkimuksen kohteena olivat kaikki kohdeyrityksen esimiehet ja toimihenkilöt. Tutkimusmetodologiana käytettiin laadullista tapaustutkimusta ja tutkimusaineistona yrityksen henkilöstöltä hankittua tietoa. Tutkimuksen perusteella voitiin luoda kuvaus osaamisen johtamisesta kohdeyrityksessä. Yrityksen henkilöstöllä on hyvä osaaminen ja esimiehet tukevat osaamisen kehittymistä. Tutkimus toi esille joitakin kehittämistarpeita. Tutkimustulosten perusteella osaamisen johtaminen ei ole vielä täysin yhtenäistä ja systemaattista toimintaa. Osaamista tulisi kehittää strategian osoittamaan suuntaan. Tutkimuksen kehittämistoimena kohdeyritykselle tehdään ehdotus osaamisen johtamisen sisältävästä johtamisjärjestelmästä.

Solving Leontief Input-Output Model with Trapezoidal Fuzzy Numbers and Gauss-Seidel Algorithm

In this work a fuzzy linear system is used to solve Leontief input-output model with fuzzy entries. For solving this model, we assume that the consumption matrix from di erent sectors of the economy and demand are known. These assumptions heavily depend on the information obtained from the industries. Hence uncertainties are involved in this information. The aim of this work is to model these uncertainties and to address them by fuzzy entries such as fuzzy numbers and LR-type fuzzy numbers (triangular and trapezoidal). Fuzzy linear system has been developed using fuzzy data and it is solved using Gauss-Seidel algorithm. Numerical examples show the e ciency of this algorithm. The famous example from Prof. Leontief, where he solved the production levels for U.S. economy in 1958, is also further analyzed.

Nurse-led interpersonal counselling for depressive symptoms in patients with myocardial infarction

The broad interest of this intervention study is in two worldwide remarkable diseases, myocardial infarction and depression. The purpose of the 18-month follow-up study was to evaluate the outcomes of interpersonal counselling implemented by a psychiatric nurse, and to examine the recovery experienced by the patients after myocardial infarction. The interpersonal counseling consisted of a short-term (max 6 sessions) depression-focused intervention modified for myocardial infarction patients. The main principle of interpersonal counselling is that depressive symptoms relate to interpersonal relations. The measured outcomes of the intervention consisted of changes in depressive symptoms and distress, health-related quality of life and the use of health care services. The data consisted of 103 patients with acute myocardial infarction and with sufficient knowledge of Finnish language, and they were randomized into intervention group (n=51) and control group (n=52) with standard care. Depressive symptoms were measured using Beck Depression Inventory, and distress using Symptom Checklist-25. The instrument to measure health-related quality of life was EuroQol-5 Dimensions. All instruments were used at three measurements: in hospital, at 6 months and at 18 months after hospital discharge. The Use of Health Care Services questionnaire was used during the 6- and 18-month period after hospital discharge. In addition, satisfaction with the intervention and with information received from the health-care professional was evaluated during the follow-up. To examine recovery, the patients kept diaries during a 6-month period and they were interviewed at 18 months after myocardial infarction. The number of patients with depressive symptoms decreased significantly more in the intervention group compared with the control group during 18 months of follow-up. Distress decreased significantly more among patients under 60 years in the intervention group than in the control group, but the difference was not significant between the groups. No differences in the changes of health-related quality of life were found between the groups during follow-up. However, in the group of patients under 60 years, the improvement of health-related quality of life in the intervention was significantly better in the intervention group compared with the control group during the follow-up. During the follow-up period, there was even a decline in the use of somatic specialized health care services in the intervention group and among intervention patients who had no other long-term disease. Considering recovery experienced by the patients, main categories including many supporting and inhibiting factors and subcategories were identified: clinical and physical, psychological, social, functional and professional category. No differences between the groups were found in satisfaction with information received from the professionals. The brief and easy-to-learn intervention, with which the patients were satisfied, seems to decrease depressive symptoms after myocardial infarction. Interpersonal counselling seems to be beneficial especially with younger patients. These results justify adopting depression screening and interpersonal counselling as part of routine care after myocardial infarction. The first stage evaluation of the use of health care services is interesting, and calls for more studies. From the perspective of individual patients, recovery after myocardial infarction seems to consist of many supporting and inhibiting factors. This is something that is important to take into account in developing nursing practice. The results indicate a need for further studies in outcomes of interpersonal counselling and recovery experienced by the patients after myocardial infarction. In addition, the results encourage widening the research perspective to nursing administration and educational level.

Suomen ja Viron tehtaiden välisen uistinliiketoiminnan suorituskyvyn ja kannattavuuden kasvattaminen

Tämän tutkimuksen tavoitteena oli selvittää Päijät-Hämeessä toimivan uistimia valmistavan tehdasyksikön sekä saman konsernin Virossa toimivan tehdasyksikön välisen uistinliiketoiminnan kannattavuuden ja suorituskyvyn mahdollinen kasvattaminen. Konsernilla on käytössään kannattavan kasvun strategia, johon tämäkin yksi liiketoiminnan kehittämishankekin omalta osaltaan myös tähtää. Kannattavuutta tarkasteltiin tehtaiden välisen päätoimialan ja sen tuottavuuden ja suorituskyvyn näkökulmasta. Työssä on lähdetty rakentamaan tehtaiden väliselle uistinliiketoiminnalle uudenlaista mittaristoa operationaalisen johdon käyttöön. Suorituskykymittaristossa otetaan huomioon suunnittelutiimin avulla määritellyt avainmittarien osa-alueet ja niiden kehittäminen tulevaisuudessa. Työ käsittelee myös hieman toimipisteissä tapahtuvan muutosten hallitsemista sekä kehittävän ilmapiirin kasvattamista. Tässä tutkimuksessa havaittiin, että suorituskyvyn mittaamisesta oli hyötyä niin operationaaliselle toimipaikkakohtaiselle johdolle kuin tuotannon työntekijällekin. Tehtaiden välisestä suorituskyvystä saatiin vertailevaa tietoa, jota voidaan käyttää hyödyksi tulevaisuudessa. Tulevaisuudessa tästä järjestelmästä saatava tieto helpottaa tuotantolaitoksissa tapahtuvaa työvaiheiden kokonaisvaltaista prosessointia josta kuitenkin pitkällä tähtäimellä suurimmat kustannussäästöt tulevat yritykselle.

Techno-Economic Feasibility of Novel On-Line Condition Monitoring Methods in Low Voltage Distribution Networks

The focus in this thesis is to study both technical and economical possibilities of novel on-line condition monitoring techniques in underground low voltage distribution cable networks. This thesis consists of literature study about fault progression mechanisms in modern low voltage cables, laboratory measurements to determine the base and restrictions of novel on-line condition monitoring methods, and economic evaluation, based on fault statistics and information gathered from Finnish distribution system operators. This thesis is closely related to master’s thesis “Channel Estimation and On-line Diagnosis of LV Distribution Cabling”, which focuses more on the actual condition monitoring methods and signal theory behind them.

Helsingin seudun liikenteenhallinnan kärkihankkeiden päivitys 2011

Työn tavoitteena oli kokonaisnäkemyksen luominen Helsingin seudun liikenteenhallinnan tilasta ja kehitysnäkymistä sekä seuraavien tärkeimpien kehittämispolkujen tunnistaminen ja konkretisointi toimenpiteiksi. Työssä keskityttiin seuraaviin painopistealueisiin 1. Liityntäpysäköinnin informaation ja maksujärjestelmien kehittäminen 2. Liikenteenhallintakeskuksen toiminnan kehittäminen ja laajentaminen 3. Liikennejärjestelmän reaaliaikainen tilannekuva ja lyhyen aikavälin ennusteet 4. Pääkaupunkiseudun pääväylien ruuhkien ja häiriöiden hallinta 5. Joukkoliikenteen ajantasaisen matkustajainformaation kehittäminen. Työssä laadittiin katsaus kullakin painopistealueella jo tapahtuneeseen sekä käynnissä olevaan kehitystyöhön, kuvattiin esiin nousseita ongelmia ja laadittiin esitys vuoteen 2020 ulottuvasta toiminnallisesta tavoitetilasta sekä konkreettisista kehittämistoimista. Työssä järjestettiin myös tulevaisuustyöpaja, johon pyydettiin alustuksia valikoiduilta viranomaistahoilta, tutkijoilta ja palveluntuottajilta. Näiden keskustelujen kautta kirkastettiin seudun toimijoiden näkemystä älyliikenteen toimialan kehitysnäkymistä ja –tarpeista. Työn lopputuloksena syntyneeseen toimenpideohjelmaan kirjattiin yhteensä 30 hanketta. Näistä viisi hanketta valittiin työpajatyöskentelyn kautta varsinaisiksi kärkihankkeiksi, joiden toteutus on kriittistä liikkujien ja viranomaistyön palvelutason kannalta ja joka edellyttää usean toimijan yhteistyötä. Lisäksi tunnistettiin joukko seurattavia hankkeita. Valitut kärkihankkeet, käynnistämisen vastuutaho sekä tavoitteellinen käynnistysvuosi ovat seuraavat: 1. Liityntäpysäköinnin dynaamisen informaatiojärjestelmän pilotointi Hämeenlinnanväylän käytävässä Kehäradan asemilla (HSL 2012) 2. HSL:n alueen joukkoliikenteen häiriönhallinnan uudelleenorganisointi (HSL, Liikennevirasto 2012) 3. Seudullisen liikenteenhallintasuunnitelman laadinta verkollisen operoinnin kehittämiseksi häiriötilanteissa (ELY-keskus, Liikennevirasto 2013) 4. Reaaliaikaisen sujuvuustiedon tuottaminen ruuhkautuvalta pääkatu- ja alempiasteiselta maantieverkolta (Liikennevirasto, kunnat 2012) 5. Liikenteen vaihtuvan ohjauksen ja tiedottamisen hyödyntäminen pääväylien ruuhkautumisen ja häiriöiden hallinnassa. (ELY-keskus 2012) Kärkihankkeisiin ei liity sellaisia riippuvuuksia, että niitä ei voitaisi käynnistää ennen päätöksentekoa jostakin muusta investoinnista. Merkittävimmät riippuvuudet liittyvät LIJ2014-järjestelmän ajoneuvojen paikannuksen ja muiden työkalujen valmistumiseen. Johtoryhmän linjauksen mukaan kärkihankkeissa ja muissakin toimijoiden omissa hankkeissa syntyvien tietojärjestelmien rajapinnat avataan soveltuvasti kaupallisten toimijoiden käyttöön, jolloin syntyvien kaupallisten loppukäyttäjäpalveluiden kautta voidaan parantaa tietopalvelujen tavoittavuutta. Kärkihankkeiden valinta ja sisältö on rakennettu pitkälti sen lähtökohdan varaan, että liikenteen tietopalveluissa julkistoimijoiden roolina on pääasiassa lähtötietojen tuottaminen ja jakaminen varsinaisille palveluntuottajille. Vuoteen 2016 mennessä uusien rajapintojen kautta jaettavien tietojen määrä on nykyiseen verrattuna huomattavasti laajempi, ja odotukset uusien ja innovatiivisten palvelujen syntymiselle ovat korkeat. HLH-johtoryhmä nimeää jokaisen kärkihankkeen läpiviennistä vastaavan työryhmän, joiden työskentelyä ohjataan nykyisen kaltaisessa johtoryhmätyössä. Johtoryhmätyön organisointi on todettu toimivaksi, sillä ryhmä koostuu seudun 14 kunnan alueen keskeisistä julkisista toimijoista ja sen kautta on olemassa vahvat kytkennät sekä seudun liikennejärjestelmätyöhön (HLJ) että valtakunnalliseen älyliikenteen kehitystyöhön.

Kullan uutto kloridiliuoksista

Työssä tutkittiin kullan neste-nesteuuttoa kloridiliuoksista kirjallisuustutkimuksen ja laboratoriokokeiden avulla. Uuttoreagenssina käytettiin Outotecin kehittämää kullanuuttoreagenssia. Teoreettisessa osassa tarkastellaan kullan käyttäytymistä happamissa vesiliuoksissa, jotka sisältävät kloridia ja bromidia. Lisäksi työssä käsitellään kultakloridiyhdisteiden käyttöturvallisuutta. Teollisuudessa vuonna 2012 käytettäviä neste-nesteuuttoon perustuvia kullan talteenottomenetelmiä esitellään lyhyesti. Kirjallisuusosassa käsitellään myös joidenkin teollisesti käytettävien polymeeristen rakennemateriaalien ominaisuuksia. Kokeellisessa osassa määritettiin kullanuuttoreagenssin fysikaalisia ominaisuuksia, kuten sen tiheys, viskositeetti, liukoisuus veteen sekä faasien selkeytyminen uuttoprosessissa. Tuloksista havaittiin, että eri modifiointiaineet vaikuttavat huomattavasti kullanuuttoliuoksen fysikaalisiin ominaisuuksiin. Kullan uuttoa tutkittiin pienen mittakaavan laboratoriokokein käyttämällä synteettisiä kultaa sisältäviä lähtöliuoksia. Kokeissa tutkittiin lähtöliuoksen happopitoisuuden ja bromidipitoisuuden, sekä uuttoreagenssin modifiointiaineiden vaikutuksia kullan uuttotasapainoon. Lisäksi tutkittiin joidenkin epäpuhtausmetallien myötäuuttautumista. Materiaalitutkimuksissa tarkasteltiin kullanuuttoreagenssin ja autenttisen kullan halidiliuoksen vaikutuksia polymeerimateriaaleihin pitkäaikaisessa kontaktissa. Uuttoliuosten havaittiin aiheuttavan turpoamaa sekä pinnan pehmenemistä joissakin teollisissa muoveissa.

Flexible multibody approach in bone strain estimation during physical activity:

Bone strain plays a major role as the activation signal for the bone (re)modeling process, which is vital for keeping bones healthy. Maintaining high bone mineral density reduces the chances of fracture in the event of an accident. Numerous studies have shown that bones can be strengthened with physical exercise. Several hypotheses have asserted that a stronger osteogenic (bone producing) effect results from dynamic exercise than from static exercise. These previous studies are based on short-term empirical research, which provide the motivation for justifying the experimental results with a solid mathematical background. The computer simulation techniques utilized in this work allow for non-invasive bone strain estimation during physical activity at any bone site within the human skeleton. All models presented in the study are threedimensional and actuated by muscle models to replicate the real conditions accurately. The objective of this work is to determine and present loading-induced bone strain values resulting from physical activity. It includes a comparison of strain resulting from four different gym exercises (knee flexion, knee extension, leg press, and squat) and walking, with the results reported for walking and jogging obtained from in-vivo measurements described in the literature. The objective is realized primarily by carrying out flexible multibody dynamics computer simulations. The dissertation combines the knowledge of finite element analysis and multibody simulations with experimental data and information available from medical field literature. Measured subject-specific motion data was coupled with forward dynamics simulation to provide natural skeletal movement. Bone geometries were defined using a reverse engineering approach based on medical imaging techniques. Both computed tomography and magnetic resonance imaging were utilized to explore modeling differences. The predicted tibia bone strains during walking show good agreement with invivo studies found in the literature. Strain measurements were not available for gym exercises; therefore, the strain results could not be validated. However, the values seem reasonable when compared to available walking and running invivo strain measurements. The results can be used for exercise equipment design aimed at strengthening the bones as well as the muscles during workout. Clinical applications in post fracture recovery exercising programs could also be the target. In addition, the methodology introduced in this study, can be applied to investigate the effect of weightlessness on astronauts, who often suffer bone loss after long time spent in the outer space.

ESTABLISH & DEPLOY DOWNSTREAM MARKETING CAPABILITIES: a case study research following the framework implementation journey

According to many academic researches, the development of marketing capabilities can enhance organizational performance. Similarly, downstream marketing capabilities have an important role in accomplishment the organizational goals. Particularly the downstream marketing capabilities identified in this research are the Marketing Communication, Selling, Marketing implementation, and Market information management. These four capabilities are summarized under the following abilities. First, the ability to manage customers’ opinion regarding the offered value from the organization. Second, the ability of the organization to obtain orders from new and established customers. Third, the ability of aligning and translate the marketing strategy into an operating action plan along with the deployment of the organizational resources. Forth, the continuous process of gathering and managing information about the markets. Moreover, the literature review of this research shed light on the elements that compose the downstream marketing capabilities. Specifically, this research examined the downstream processes and the required information required to control these processes based on the American Productivity and Quality Center’s Process Classification Framework. Furthermore, the literature review examined some of the technological tools that are used in marketing processes, and also some managerial implication regarding the management of the downstream marketing employees. Along with the investigation of downstream marketing capabilities, the literature review investigated the utilization and the benefits of Component Business Model and Process Classification Framework, as they are defined by the organizations that developed them. Besides this initial study, the research presents how the examined organization is using the two frameworks together by cross-referring them. Finally, the research presents the optimal deployment of the collected downstream capabilities elements in the current organizational structure. The optimal deployment has been grounded on the information collected from the literature review but also from internal documentation, provided from the examined organization. By comparing the optimal deployment and the current condition on the organization, the research exhibits some points for improvement, but also some of the projects that are currently in progress inside the organization and eventually will provide solutions to these downsides.