Análise do gene TUBB3 e de Copy Number Variations em pacientes com Polimicrogiria

A polimicrogiria (PMG) é uma malformação do córtex cerebral causada por falhas no seu desenvolvimento, caracterizando-se por um número excessivo de pequenos giros e laminação anormal, dando à superfície cortical uma aparência irregular e grosseira. A gravidade de suas manifestações clínicas se relaciona diretamente com a extensão da malformação e das regiões cerebrais afetadas, sendo que a presença de lesões bilaterais ou unilaterais extensas indica um pior prognóstico. Uma das síndromes de polimicrogiria mais freqüentes e, conseqüentemente, mais bem descritas clinicamente, é a polimicrogiria perisylviana bilateral (PPB). Essa forma de PMG atinge a região que tange a fenda Sylviana, podendo apresentar-se tanto unilateralmente quanto em ambos os hemisférios. Vários genes têm sido relacionados a diferentes formas de polimicrogiria, são eles AFF2,TUBA1A, TUBB2B e TUBA8, SRPX2 e WDR62. Estes genes já foram estudados pelo nosso grupo de pesquisa em um grupo de pacientes compostos de casos familiares e esporádicos, acometidos em sua maioria pela forma perisylviana de PMG. Nenhuma variante deletéria foi identificada nestes genes. Recentemente um novo gene foi implicado na etiologia molecular das PMG, o TUBB3. O gene em questão pertence à mesma família de TUBA1A, TUBB2B e TUBA8 e codifica uma proteína de ligação aos microtúbulos, tendo importante papel na formação do fuso. Além deste gene, também tem sido descritas alterações genômicas, denominadas de Copy Number Variations (CNV), estas variações estruturais tem sido associadas com diversos distúrbios neurológicos, que vão desde transtornos psiquiátricos até malformações do córtex cerebral como a PMG. Desta forma, o objetivo deste trabalho foi analisar a existência de alterações de ponto deletérias no gene TUBB3 em pacientes com PMG e também, o envolvimento de CNVsna etiologia deste tipo de malformação ...

Deslocamento do abomaso à esquerda em bovinos leiteiros: da etiologia ao diagnóstico

A pecuária leiteira vem sofrendo ao longo dos últimos anos, constantes mudanças com o intuito de maximizar a produção. A contínua seleção para uma maior produção de leite em conjunto com o aumento da capacidade digestiva e profundidade corporal aumentou a susceptibilidade de ocorrência de várias enfermidades abomasais, como o deslocamento do abomaso. O deslocamento de abomaso é uma doença economicamente importante na bovinocultura leiteira e é definido como uma síndrome multifatorial onde há basicamente duas possibilidades de deslocamento, na primeira, a víscera pode deslocar-se à esquerda, causando o deslocamento do abomaso à esquerda e a segunda possibilidade, o órgão pode mover-se totalmente para o lado direito, provocando o deslocamento do abomaso à direita, sendo que o primeiro ocorre mais frequentemente. O período de maior ocorrência do deslocamento do abomaso à esquerda, se insere imediatamente ou até seis semanas após o parto. Assim, objetivou-se com essa revisão sistemática, elucidar as principais causas e fatores de risco, os variados sinais clínicos e os diversos métodos diagnósticos dessa enfermidade. Conclui-se que, é de extrema importância conhecer desde a causa, até o diagnóstico do deslocamento do abomaso à esquerda, para que auxilie os médicos veterinários a identificarem o problema na propriedade, direcioná-lo a um tratamento adequado e tomar medidas preventivas, eliminando os fatores de risco e consequentemente diminuindo a incidência do problema.

Cárie oculta extensa: relato de caso clínico

The pattern of caries lesions has been modified over decades. If the presence or absence of a caries lesion is based upon only a visual examination, its diagnosis might become underestimated since dentinal lesions underneath non cavitated or minimally demineralized enamel is highly frequent in dental clinics. The aim of this paper is to report a clinical case of a child with an upper molar with questionable occlusal aspect, presenting asymptomatic pulpal hyperplasia on the distolingual pit. Periapical radiographic examination was essential for diagnosing hidden caries and elaborating the treatment plan. As part of the approach, removal of the decayed tissue and pulpotomy with calcium hydroxide were performed. The association of an accurate visual examination to an adequate oral radiographyis is extremely important in cases which the presence of hidden caries is doubtful. As a result, an early diagnosis and the reduction of more invasive interventions related to the oral health care of pediatric patients can be achieved as well.

Contaminación microbiana en las líneas de agua de las unidades odontológicas

Introduction and Objective: Microorganisms are responsible for multiple infections and pathologies; this is why it is important to control microbes that can be found in the triple syringe used for irrigation in different dental procedures. The aim of this study was to identify cultivable species of microbes (fungus and bacteria) found in some dental units water lines of a private dental clinic from Medellín, Colombia. Materials and Methods: Random samples were taken from 11 chairs from a total of 89; a sample of water of 500ml was collected from the triple syringe of each selected chair. The study aim to search for the presence of total coliforms, filamentous fungi and leavened Results: the average presence of microorganisms was between 40CFU and more of 200 CFU. Microorganisms such as Aeromona salmonicida, Actinobacilus sp and Pseudomona maltophil were isolated. No total coliforms neither fecal coliforms were found. Conclusions: the high levels of contamination suggest that there is a mature biofilm in somewhere of the dental unit water line, but the absence of total and fecalis coliforms suggest that the water had been treated.

Infra-oclusão de molares decíduos: diagnóstico e tratamento

Infraocclusion of deciduous molars is defined by total or partial periodontal ligament absence and characterized by the cementum or dentin anatomical fusion with alveolar bone. This study aims to report an ankylosis clinical case, the implications and treatments management options in the deciduos dentition.

Identificação molecular por reação em cadeia da polimerase multiplex para espécies do gênero Candida de importância médica

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Relação entre presença de anticorpos anti-leishmania spp. e carga parasitária em sangue de cães de área endêmica para leishmaniose visceral

Pós-graduação em Ciência Animal - FMVA

Percepción de un grupo de mujeres acerca del hecho de ser portadoras del VPH

Background: The possibility to acquire sexually transmitted diseases generates bio-psycho-social conflicts affecting daily life of women. Objective: To investigate the knowledge of women on the concept of being infected by the human papilloma virus (HPV) and to understand the significance they attributed to their condition as HPV carriers. Material and method: It is a qualitative study in which ten women were interviewed, after the result of a cytopathological exam suggesting HPV. Data were collected at the Center of Health School of the Universidade Estadual Paulista -UNESP-Brazil. Results: The significant items were: deception and preoccupation about the cure, since they indicate that it is very deceptive to be infected with an incurable disease by a person whom they trust; the method of having sexual relations and being infected again; the necessity of care and of being optimistic to face the disease and the threat of cervix cancer originating anxiety. Discussion: Orientations made by health professionals were efficacious to clarify the relationship with cancer development. It is possible to understand the necessity of assistance orientations and the opportunity to hear them, offering individualized quality care. This study also offers important elements to reinforce the educational role of health professionals, principally those referring to sexually transmitted infection and cancer, seeking prevention and early treatment with the offered information. However, there is no desire of finishing the subject regarding perception, thus there is much to discover about diseases related to human papilloma virus. Key words: women carriers of HPV, feelings attributed by women.

A flow cytometry method for testing the susceptibility of Cryptococcus spp. to amphotericin B

Human fungal infections have increased at an alarming rate in recent years, particularly in immunocompromised individuals. Cryptococcosis is the second most prevalent systemic fungal infection worldwide, and the most prevalent systemic infection in immunocompromised individuals, representing more than 70% of cases. The incidence of cryptococcosis is high in people with HIV/acquired immunodeficiency syndrome (AIDS), with recent estimates indicating that there are one million cases of cryptococcal meningitis globally per year in AIDS patients. The aim of this research was to develop a rapid flow cytometric antifungal susceptibility test and to compare the results with the standard methods. A reference strain and clinical isolates of Cryptococcus neoformans and Cryptococcus gattii were tested for susceptibility to amphotericin B by flow cytometry using propidium iodide as indicator of viability. Flow cytometry (FC) results were compared with the minimum inhibitory concentration (MIC) values determined by microdilution. The antifungal activity of amphotericin B ranged from MICs of 0.06 to 2μg/ml for the 11 isolates studied. The same results were found by FC. The FC method allows same-day results, assisting in the selection of appropriate antifungal therapies. These results demonstrate an excellent correlation between FC and the classic methods of testing for susceptibility to antifungal agents. This rapid diagnosis method makes it possible to quickly administer effective therapeutic interventions, often saving lives.

Genotipagem dos isolados de Giardia duodenalis em famílias de pescadores da colônia de Porto Said, Botucatu, São Paulo

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Emprego de redes complexas para modelar e analisar a disseminação de microrganismos da familia enterobacteriaceae em pacientes mantidos em unidades de terapia intensiva

Nowadays the studies of different methodologies to interfere in the growing and spread of serious infections and systemic status in institutionalized patients those kept on intensive therapy units are relevant to understanding these complex systems and bring benefits to several health areas, particularly public health. In this study, it was analyzed the clinical and microbiological data from patients hospitalized in intensive therapy units. The interaction between patients and caregivers was modeled and analyzed using dynamic system model and complex network theory, identifying outbreaks values of microorganisms of Enterobacteriaceae Family.

Speech-language therapy for treatment of dysphagia and dysphonia in systemic granulomatous diseases: granulomatous diseases: dysphagia and dysphonia treatments

The granulomatous lesions are frequently founded in infectious diseases and can involve the larynx and pharynx and can cause varying degrees of dysphonia and dysphagia. There is still no systematic review that analyzes effectiveness of speech therapy in systemic granulomatous diseases. Research strategy: A systematic review was performed according to Cochrane guideline considering the inclusion of RCTs and quasi-RCTs about the effectiveness of speech-language therapy to treat dysphagia and dysphonia symptoms in systemic granulomatous diseases of the larynx and pharynx. Selection criteria: The outcome planned to be measured in this review were: swallowing impairment, frequency of chest infections and voice and swallowing symptoms. Data analysis: We identified 1,140 citations from all electronic databases. After an initial shift we only selected 9 titles to be retrieved in full-text. After full reading, there was no RCT found in this review and therefore, we only described the existing 2 case series studies. Results: There were no randomized controlled trials found in the literature. Therefore, two studies were selected to be included only for narratively analysis as they were case series. Conclusion: There is no evidence from high quality studies about the effectiveness of speech-language therapy in patients with granulomatous diseases of the larynx and pharynx. The investigators could rely in the outcomes suggested in this review to design their own clinical trials.

Avaliação do grau de conhecimento sobre Leishmaniose e Toxoplasmose em moradores do município de Araçatuba, SP

Considering the importance of zoonotic infections by Leishmania spp. and Toxoplasma gondii in Araçatuba region, São Paulo, this study determined the degree of knowledge of residents of Araçatuba about these two diseases. Questionnaires about these diseases were applied to 123 people. Regarding schooling, all the respondents were literate, and 69.9% (86/123) did not attend higher education and 30.8% (37/123) completed an undergraduate degree. Of these, 91.9% (34/37) knew the meaning of the term zoonosis, with significant difference compared to those who were not trained. This word was unknown by 57% (29/123) of total respondents. Most non-graduates, 59.3% (51/86) did not know what is toxoplasmosis and among graduates, 35.1% (13/37) ignored the subject.There were significant difference between these groups. Regarding the prevention of Leishmania infection, a greater proportion of the residents advocated environmental cleanup, the collar repellent and use of citronella. In relation to toxoplasmosis, 54.5% did not know the ways of transmission of this disease and among those with graduate degree, 13 ignoring any transmission medium. When it comes to prevention of this disease, 55.3% (68/123) did not know any prophylactic. In the group of higher education, 14 did not know how to prevent toxoplasmosis. The results of this study show that the majority of respondents are not aware about the mode of transmission of these diseases, especially in relation to toxoplasmosis.

Dor orofacial

The orofacial pain, also known by the generic term dysfunction, is highly prevalent in the general population, causing great discomfort to the patients. For this reason, there is great importance of dentist’s participation in practice and in the process of proper diagnosis of this disease, which in modern times has become increasingly important as guides the best treatment. Thus, this study was aimed to review the main causes, diagnosis, main symptoms and treatment modalities, to serve as a guide to the dentist. Moreover, it also aims to review the main etiologic factors commonly associated, as emotional stress and muscular hyperactivity, to clearly identify signs and symptoms, so the dentist is able to realize simple treatment in your particular office or recognizes complex cases that could be send for treatment to multidisciplinary teams.

Aspectos oclusais na etiologia das desordenstemporomandibulares

Since the occlusion area is presented in almost all specialties of Dentistry, its relationship with the health of the patient has been shown. The etiology of temporomandibular disorders is multifactorial and the occlusion is considered an etiologic factor. This study aimed to discuss the functional occlusion aspects and the presence of temporomandibular disorders. Literature Review: The loss of orthopedic balance can induce temporomandibular disorder. Therefore, both the physiological and functional occlusions have been analyzed as a dental relation. Several functional occlusal factors have been linked to the temporomandibular disorders such as deviations of centric relation to maximum intercuspation, occlusal interferences, absence of posterior teeth, changes on the occlusion vertical dimension, malocclusion, orthodontic treatment and parafunction. Conclusion: The most common occlusal factors related to temporomandibular disorders are quite frequent; however, the diagnosis and treatment of such disorders should be considered individually