Diversidade genética do sistema HLA em Portugal, Cabo Verde e Guiné-Bissau

O polimorfismo dos genes HLA-A, HLA-B e HLA-DRB1 foi estudado em três populações Portuguesas (Portugal continental, Madeira e Açores) e em duas Africanas (Guiné-Bissau e Cabo Verde). Os dados em alta resolução, obtidos por sequenciação (SBT), foram comparados com a caracterização efectuada pelo método SSOP revelando 4,6% de incongruências entre os resultados destes dois métodos. Os alelos mais frequentes em cada um dos loci foram: HLA-A*0201 em todas as populações (13,5-26%); HLA-B*5101 em Portugal continental (12%, o mesmo para o B*440301), Madeira (9,7%) e Açores (9,8%) e B*350101 na Guiné-Bissau (14,4%) e Cabo Verde (13,2%); HLADRB1*0701 em Portugal continental (15%), Madeira (15,7%) e Açores (18,3%), DRB1*1304 na Guiné-Bissau (19,6%) e DRB1*110101 em Cabo Verde (10,1%). Os haplotipos 3-loci mais predominantes em cada população foram: A*020101-B*440301-DRB1*070101 em Portugal continental (3,1%), A*020101-B*510101- DRB1*130101 na Madeira (2,7%), A*2902-B*4403-DRB1*0701 nos Açores (2,4%), A*2301-B*1503-DRB1*110101 na Guiné-Bissau (4,6%) e A*3002-B*350101-DRB1*1001 em Cabo Verde (2,8%). O presente trabalho revela que a população continental Portuguesa tem sido influenciada geneticamente por Europeus e Norte Africanos devido a várias imigrações históricas. O Norte de Portugal parece concentrar, provavelmente devido à pressão da expansão Árabe, um antigo pool genético originado pela influência milenar de Europeus e Norte Africanos. Os dados obtidos nos genes do sistema HLA corroboram as fontes históricas que confirmam que o povoamento dos Açores teve o contributo de outros Europeus, essencialmente Flamengos, para além dos Portugueses. As frequências alélicas e haplotípicas neste arquipélago não apresentam uma distribuição homogénea entre as ilhas do grupo Oriental e Central. O grupo Central revela uma influência clara daEuropa Central e uma muito menor afinidade a Portugal continental. As frequências alélicas e haplotípicas mostram que a ilha da Madeira foi povoada por Europeus, a maioria Portugueses, mas também por sub-Saharianos devido ao comércio de escravos. Cabo Verde não é uma população tipicamente sub-Sahariana pois revela uma importante influência genética Europeia, para além da base genética Africana. A análise dos haplotipos e dendrogramas mostram uma influência genética Caucasiana no actual pool genético Cabo-Verdiano. Os dendrogramas e a análise das coordenadas principais mostram que os Guineenses são mais semelhantes aos Norte Africanos do que qualquer outra população sub-Sahariana já estudada ao nível do sistema HLA, provavelmente devido a contactos históricos com outros povos, nomeadamente Árabes do Este Africano e Berberes.

Filhos da independência: etnografando os estudantes bissau-guineenses do PEC-G em Fortaleza-CE e Natal-RN

Notre travail propose de comprendre l`immigration africaine contemporaine du Brésil à travers l'univers des étudiants guinéens participant au Programme Étudiants « Convênio de Graduação » (PEC-G) dans les villes de Fortaleza dans l'Etat Ceará et Natal dans l'Etat de Rio Grande do Norte, en étudiant leurs stratégies de convivialité et d'adaptation. Les étudiants étrangers sélectionnés dans ce programme font leur licence gratuitement dans les Instituts d'Enseignement Supérieur (IES). Pour accéder à ce programme ils doivent répondre à certains critères : montrer qu'ils sont en mesure de payer leurs frais au Brésil, avoir le bac ou un diplôme équivalent et maîtriser la langue portugaise lorsqu'ils sont originaires d'un pays n'appartenant pas à la Communauté de Pays de Langue Portugaise (CPLP). Les étudiants qui participent à des programmes de développement socio-économique contractés entre le Brésil et leurs pays d'origine sont prioritaires. Ces accords les contraignent à rentrer dans leurs pays d'origine et de travailler dans le domaine dans lequel ils ont été diplômés une fois les études terminées. Les étudiants, qui arrivent au Brésil porteurs de leurs identités ethniques guinéennes, entrent en contact avec la société brésilienne et acquièrent ainsi une « identité hybride ». C'est dans ce contexte que ce travail analyse le quotidien des étudiants africaines au Brésil avec un regard porté sur les étudiants de Guinée-Bissau à Fortaleza et à Natal comprendre l'expérience des étudiants qui vivent en terre étrangère. Ainsi, le lieu (Brésil) prend toute sa valeur par rapport au distant (Guinée) autrefois lié par l'histoire coloniale et aujourd'hui lié par des relations internationales ou diplomatiques. Finalement, la construction d'une « nouvelle » identité ethnique, d'une culture guinéenne au Brésil s'opère dans une célébration mobile c'est-à-dire successivement formée et transformée en relation avec les formes à travers lesquelles l'individu est représenté ou interpelé dans les systèmes culturels dans lesquels il est impliqué.

Situações de Risco, Trabalho e Saúde de Imigrantes Brasileiros: representações sociais

This study searches in approaching diverse pertinent aspects to the immigration process that affects the countries of the European Union especially in Portugal. The works deriving from this research has its objective in: analyzing the risk, work and health in Brazilian immigrants residing in Lisbon, Portugal. As methodological way, we use the instruments: Test of Free Association of Words and half-structuralized interview. For in such a way, they had been processed in software s for analysis between them: SPSS 14,5, Evoc, Trideux and Alceste. The not-boarded results in this study will be worked as clippings and will be sent for posterior publication. Thus, I consider this work enriching, in view of contributing of the same as mechanisms of understanding of being a immigrant and the possibility of the mobilization of the society and the academic environment for a phenomenon growing each time more, especially, the professionals of health, so we can intervine in a more necessary form within the factors that affects this population layer directly

Fatores genéticos e étnicos relacionados à evolução da infecção por Leishmania infantum

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior

Modos de ser e modos de viver : a trajetória dos estrangeiros em Natal/RN

This work aims to understand the trajectory of immigrants living in Natal / RN, between the years 1990 and 2009, their motivations for this change, their sociability relations, as well as the impact of this group in Natal and specifically, in the district of Ponta Negra, where most of them established their residences or work. Considering that the historical, political, economical and social environment where these immigrants are inserted, are different from that experienced by former immigrants who came to Brazil in the late nineteenth and early twentieth centuries, a period of mass migration, it was revealed that immigration studied in contemporary Natal constitutes a new configuration (Elias, 1994). This new configuration is represented by several characteristics that distinguish this contemporary immigration, as: the relationship established by these foreigners, both with their country of origin and the destination, the formation of social networks that offer support to newcomers and those already installed, the relations of sociability with locals and a new relationship of belonging with the space, since the possibility of coming and going from one country to the other, allows a (re)production of their original ?? social and economic life in the new space. The research methodology was based on the analysis of narrative interviews, in light of the theory of Schütze (1977) in order to understand the faces of this immigration. As theoretical support for the data analysis the theories of authors such as Norbert Elias, presentedin his work about the established and outsiders, Pierre Bourdieu, and his concept of habitus, distinction and style of life, Georg Simmel, and his study of life in the metropolis, his analysis of the foreigner and his theory of sociability, among others. This study attempted to give visibility to foreigners living in Natal, who experience the dynamics of the district of Ponta Negra and reconstruct their daily relations of sociability in this space. In fact, the presence of foreigners in this urban context allows for the construction of new configurations, both in terms of the physical space of the neighborhood, which has been modified by the touristification process and the real estate market, which is molded to meet this demand, but also in their social sphere, with regard to the relations established between foreign residents and locals. In seeking to characterize these migratory experiences it could be understood that the trajectories of each foreigner is part of a broader social structure, which cannot be evaluated neither disconnected from the contexts in which they operate, nor under a single point of view

ÁFRICA(S), MOÇAMBICANIDADE, MIA COUTO: uma varanda para o Índico

Always seen by others eyes, unknown of the languages and other "realities" that constitute, the Africa, specially Mozambique, now (re)invent itself, palimpsestly, in narratives of your griots, narrators and/or storytellers. Our thesis intend, by the review of novel Under the frangipani, to demonstrate that the mozambican writer Mia Couto intends to participate the (re)construction of a devasted nation by war and conflicts, based on belief that the role writer "is to create requisites of a more african thought, to the assessment of your place and your time won't be done from categories created by others" (COUTO, 2005). At first, we show how the mozambican literary system consists itself; then, the insertion of coutian narrative against the prevalence tradition and, also, by the analysis of the constituent elements of the chosen novel, the way how made piece of resistance to reification, objectification of the individuald in the contemporary world , your writing will be present in this sharing of the sensitive. Besides Mia Couto, in our work we'll counting on the lessons of Candido, Noa, Hernandez, Adorno, Paz, Fonseca, Secco, among others studious of african narrative and, specially, Mozambique narrative

Entre fronteiras: a escrita imigrante de Julia Alvarez em How the García girls lost their Accents

This master s thesis aims at investigating the way in which diasporic subjects in the novel How the García Girls Lost their Accents (1992) cope with the clash of two cultures the Caribbean one, from the Dominican Republic, and the North-American one, from the U.S., as well as the implications of such negotiations in the lives of immigrants, once it apparently depicts the plight of those who are torn between mother-lands and mother-tongues (IYER, 1993, 46). At the same time, the implications of such negotiations in the lives of immigrants are relevant issues in the writing of Julia Alvarez. For this, there is the analysis of the uses of family memories as one of the main strategies immigrant writers possess to recall their identities. Moreover, this thesis will also consider the language issue for the construction of the immigrant identity insofar as bilingualism is a key factor in the negotiation the García girls must effect between their Caribbean and their American halves in order to understand where they stand in the contemporary world. In order to build a theoretical framework that supports this master s thesis, we list the works of Homi K. Bhabha (1990, 1996, 2003, 2005), Stuart Hall (2001, 2003), Julia Kristeva (1994), Salman Rushdie (1990, 1994), Sonia Torres (2001, 2003) among other contributions that were crucial to the completion of this academic research

Atividade da 6-fosfogliconato desidrogenase em deficientes de glicose-6-fosfato desidrogenase

As enzimas G6PD e 6PGD são responsáveis pela geração do aporte de NADPH, necessário para a detoxificação dos agentes oxidantes produzidos pelo estresse oxidativo metabólico nos eritrócitos. Devido à alta prevalência de deficiência de G6PD na população mundial, principalmente de origem negróide africana, muitos estudos têm sido realizados na tentativa de conhecer melhor a atuação destas enzimas. O objetivo deste estudo foi avaliar a atividade enzimática da 6PGD, nos deficientes de G6PD, para verificar a existência de aumento da atividade desta enzima, correlacionando com um possível aumento do número de reticulócitos ou presença de alterações da série vermelha. A pesquisa em 2.657 indivíduos do sexo masculino resultou em 97 deficientes de G6PD, determinando uma prevalência de 3,65% para a região de Bauru (SP), com atividade enzimática média de G6PD de 1,74 UI.g Hb-1. min-1 a 37ºC, 14,4% da atividade da G6PD normal. A atividade enzimática média da 6PGD foi de 9,5 UI.g Hb-1. min-1 a 37ºC, estando aumentada em 47,4% dos deficientes de G6PD. Os resultados não confirmaram que a hipótese do aumento da atividade enzimática da 6PGD, em deficientes de G6PD, seja decorrente da presença de um número aumentado de reticulócitos na corrente circulatória, faixa etária ou alterações eritrocitométricas que denotem anemia. O mais provável é que a hemólise autolimitada, imposta pelos processos oxidativos, preserve os eritrócitos mais jovens, que possuem atividade enzimática mais elevada, uma vez que naturalmente ocorre diminuição da atividade destas enzimas com o envelhecimento celular.

Determinação de haplótipos do gene βs em portadores de anemia falcifome

Haplotypes linked to the βS gene represent patterns of DNA polymorphisms along chromosome 11 of individuals bearing the βS gene. Analysis of haplotypes, in addition to serving as an important source for anthropological studies about the ethnic origin of a population, contributes to a better understanding of the variations in clinical severity of sickle cell anemia. The aim of the present study was to determine βS gene haplotypes in a group of patients with sickle cell anemia treated at the Dalton Barbosa Cunha Hematology Center (Hemonorte) in Natal, Brazil and the Oncology and Hematology Center in Mossoró, Brazil. Blood samples were obtained from 53 non-related patients (27 males and 26 females), aged between 3 months and 61 years (mean age: 16.9 ± 12.1 years). Laboratory analyses consisted of the following: erythrogram, reticulocyte count, hemoglobin electrophoresis at alkaline pH, measurement of hemoglobin A2 and Fetal hemoglobin, solubility test and molecular analysis to determine βS gene haplotypes. DNA samples were extracted by illustra blood genomicPrep Mini Spin kit and βS gene haplotypes were determined by PCR-RFLP, using Xmn I, Hind III, Hinc II and Hinf I restriction enzymes for analysis of six polymorphic restriction sites in the beta cluster. Of 106 βS chromosomes studied, 75.5% were Central African Republic (CAR) haplotype, 11.3% Benin (BEN) and 6.6% Cameroon (CAM). The atypical haplotypes had a frequency of 6.6%. More than half the patients (58.5%) were identified as CAR/CAR genotype carriers, 16.9% heterozygous CAR/BEN, 13.2% CAR/CAM and 1.9% BEN/BEN. Patients with atypical haplotype in one or two chromosomes accounted for 9.5% (CAR/Atp, BEN/Atp and Atp/Atp). The genotype groups showed no statistically significant difference (p < 0.05) in their laboratory parameters. This is the first study related to βS haplotypes conducted in state of Rio Grande do Norte and the higher frequency of Cameroon halotype found, compared to other Brazilian states, suggests the existence of a peculiarity of African origin

Prevalência de talassemia alfa+ (deleção -3.7) na população adulta do estado do Rio Grande do Norte

Alpha thalassemia, the most common monogenic disorder in the world, is characterized by deletions of one (+-thalassemia) or both alpha genes (0-thalassemia) located on human chromosome 16 (16p13.3). The most common case of +-thalassemia is a deletion of 3.7 kb of DNA (-3.7 deletion). It is most prevalent in African and Middle East regions. In the few studies carried out in Brazilian population -3.7 deletion was the most common deletion, mainly in African descendants. This study was conducted to determine the prevalence of +- thalassemia (deletion 3.7kb) in adult population from Rio Grande do Norte. We obtained blood samples from 713 unrelated individuals of both genders, aged between 18 and 59 years old. All individuals were born in Rio Grande do Norte. The hematological indices were obtained in an automatic cell counter (Micros 60, ABX Diagnostics). The hemoglobin measurement (A2 and Fetal hemoglobin) and the profile confirmation were carried out by high performance liquid chromatography (HPLC) methodology. Genomic DNA was obtained from peripheral blood leukocytes using Illustra Blood GenomicPrep Mini Spin kit and -3.7 deletion was investigated by PCR. Among the 713 individuals studied, 80 (11,2%) presented +- thalassemia: 79 (11,1%) were heterozygous and 1 (0,1%) homozygous for the -3.7 deletion. Considering the ethnic group, negroes showed the greatest prevalence of +-thalassemia (12,5%), followed by mulattoes (12,3%) and caucasian (9,6%). Statistical comparison of hematological parameters between normal individuals and heterozygous to +-thalassemia showed significant differences in RBC (p<0,001), MCV (p<0,001), MCH (p<0,001), Hb A2 (p=0,007) as well as female hemoglobin concentration (p=0,003). This is one of the first studies to research +-thalassemia in general population of Rio Grande do Norte state and these results attest the importance of investigation of this condition to define the etiology of microcytosis and hypochromia.

Caracterização morfológica e sedimentologica da plataforma, continental brasileira adjacente aos municípios de Fortim, Aracati e Icapuí- CE

The study area is located on the Brazilian Continental Shelf adjacent to Ceará State, inserted in the submerged Potiguar Basin. This area was submitted to extensional efforts during Upper Cretaceous, associated to the begining of the rifting that resulted in African and South American Continent separation. The main goal of this research was to better understand the sedimentary and geomorphological characteristics of the continental shelf adjacent to Fortim, Aracati and Icapuí (Ceará State). The used data base included geophysical (sides scan sonar and bathymetry studies) and sedimentological survey, associated to satellite image processing and interpretation. Inferences about suspended material and longshore drift was possible using satellite images, and differente bedforms were characterized such as: different kinds of dunes (longitudinal, cross and oblique), bioclastic banks, paleochannels, flat and rock bottom. The researched area comprehended about 2509,13 km2, where 6 different sedimentary facies, based on sediment composition and texture, could be recognized, such as: Bioclastic Sand, Siliciclastic Sand, Biosiliciclastic Sand, Bioclastic gravel, Biosiliciclastic sand with granule and gravel, and Silicibioclastic sand with granule and gravel. The integration of bathymetric, satellite image, side scan sonar and sedimentological data allow us a better characterization of this continental shelf area, as to advance in the knowledge of the continental shelf of the state of Ceara, a very important area to the oil industry because of its potential exploration and e exploitation, and to environmental survey as well