Bioquímica molecular como ferramenta para conservação e uso da biodiversidade agrícola tropical: desenvolvimento de marcador imunoquímico para identificação da bactérias endofíticas do milho.

Marcadores moleculares estão sendo amplamente utilizados na agricultura. Entre as várias aplicações práticas, destacam-se a identificação de raças, linhagens e estirpes, eliminação de réplicas em bancos de germoplasma, identificação de genes associados com a performance da planta, entre outros. Em todos esses casos, a definição da estratégia que deverá ser adotada precisa considerar os custos para a obtenção dos marcadores. Ênfase crescente tem sido dada para identificar marcadores menos onerosos. Marcadores bioquímicos constituem um tipo especial de marcador molecular que se caracteriza pela análise do produto da expressão gênica. Destacam-se dois tipos principais: isoenzimas e proteínas. Esses marcadores também são definidos como marcadores fenotípicos, pois podem resultar da interação genótipo/ambiente. Embora de aplicação limitada, o uso desses marcadores em algumas áreas ou em casos especiais fornece informações seguras e úteis para a identificação de indivíduos. Recentemente, as técnicas de SDS-PAGE, RAPD-PCR, ARDRA e seqüenciamento de genes ribossomais foram empregadas no Laboratório de Bioquímica Molecular de Microrganismos da Embrapa Milho e Sorgo, para identificar bactérias endofíticas isoladas do milho. Naquele estudo, foi verificada, em todos os isolados, a presença de um polipeptídio de aproximadamente 42 kDa e cuja expressão era bastante elevada. O objetivo deste trabalho consistiu em aprofundar os estudos sobre o polipeptídio de 42 kDa, visando o desenvolvimento de marcadores imunoquímicos para identificar e estudar a dinâmica de colonização bactérias endofíticas em plantas de milho.

Strategic intelligence, Counter-Intelligence and Alliance Diplomacy in Anglo-French relations before the Second World War

Jackson, Peter, and Joe Maiolo, 'Strategic intelligence, Counter-Intelligence and Alliance Diplomacy in Anglo-French relations before the Second World War', Military History (2006) 65(2) pp.417-461 RAE2008

What children learn from adults’ utterances: an ephemeral lexical boost and persistent syntactic priming in adult–child dialogue

We show that children’s syntactic production is immediately affected by individual experiences of structures and verb–structure pairings within a dialogue, but that these effects have different timecourses. In a picture-matching game, three- to four-year-olds were more likely to describe a transitive action using a passive immediately after hearing the experimenter produce a passive than an active (abstract priming), and this tendency was stronger when the verb was repeated (lexical boost). The lexical boost disappeared after two intervening utterances, but the abstract priming effect persisted. This pattern did not differ significantly from control adults. Children also showed a cumulative priming effect. Our results suggest that whereas the same mechanism may underlie children’s immediate syntactic priming and long-term syntactic learning, different mechanisms underlie the lexical boost versus long-term learning of verb–structure links. They also suggest broad continuity of syntactic processing in production between this age group and adults.

W poszukiwaniu czasowników nieznanych lingwist(k)om

Abstract. The paper presents a list of 437 verbs which have not been recorded in lexicography. As the source of reference, the author consulted a spelling dictionary of the Polish language, Wielki słownik ortograficzny PWN, 2nd edition, 2006. The concept of this paper originated from the wish to satisfy the curiosity after reading Indeks neologizmów [Index of Neologisms] prepared by Krystyna Waszakowa in her work Przejawy internacjonalizacji w słowotwórstwie współczesnej polszczyzny [Word-formative internationalisation processes in modern Polish]. The index contains a list of nouns. Given that K. Waszakowa did not take verbs into account (there are far (?) fewer neo-verbs than neo-nouns), the author decided to find out whether it is true that the number of verb neologisms is so small that their philological analysis is pointless from the point of view of research, vocabulary registration, etc. If nouns, such as podczłowiek, miniokupacja, redefinicja, are of interest, why not record the prefixal constructions of the do-, z-, od-, na-, w-, wy-, za-, od-, nad- etc. -robić type? The analysis included randomly selected texts from the „Rzeczpospolita” daily (without any thorough preparation with respect to the content; the texts available were sequentially analysed until the satisfactory result was obtained). The texts under review included an incomplete (it is virtually impossible to determine completeness in this case) electronic archive from the years 1993–2006.

Analogia w dydaktyce języka chińskiego

W artykule zajmuję się problematyką dydaktyki wybranych konstrukcji języka chińskiego. Moim celem jest pokazanie na przykładach, w jaki sposób, wykorzystując zarówno różnice semantyczne między zdaniami strukturalnie podobnymi, jak i analogie semantyczne między zdaniami składniowo różnymi, ułatwić i przyspieszyć nauczanie problematycznych w tym względzie zagadnień gramatyki chińskiej. Do egzemplifikacji wykorzystania mechanizmów różnic i analogii w dydaktyce wykorzystałem zdania z partykułą le i skopiowanym czasownikiem, konstrukcję z de i dopełnieniem komplementywnym oraz partykułę końcową le.

O Estágio como cenário de prospecção, desenvolvimento e concepção de um Manual de Procedimentos da Rede Concelhia de Bibliotecas Escolares

Dissertação de Mestrado apresentada à Universidade Fernando Pessoa como parte dos requisitos para obtenção do grau de Mestre em Ciências da Informação e da Documentação.

Aconselhamento farmacêutico em otorrinolaringologia

Monografia apresentada à Universidade Fernando Pessoa para obtenção do grau Licenciada em Ciências Farmacêuticas

Estudo do comportamento no âmbito da radioprotecção de uma população trabalhadora na área da medicina dentária

Monografia apresentada à Universidade Fernando Pessoa como parte dos requisitos para obtenção do grau de Licenciada em Medicina Dentária

Os objectivos políticos da OEA

A América Latina atravessa, actualmente, significativos momentos de avanço e de recuo na sua estabilidade política interna. Os conflitos políticos da região e os regimes recentemente instalados em alguns Estados poderão criar situações de um permanente adiamento do desenvolvimento económico e de garantias democráticas de protecção dos direitos humanos. Na falta de consenso entre os Estados e a sua própria incapacidade de intervenção, a Organização dos Estados Americanos deverá assumir a sua missão e intervir no sentido de concretizar a estabilidade política necessária ao progresso económico e ao incremento de relações pacíficas no continente. Nowadays, Latin America lives significant periods of advance and retreat in its internal political stability. The political conflicts in the region and the regimes recently installed in some states will conduct to situations of a permanent postponement as to the economic development and democratic guarantees in the protection of human rights. While a consensus between states is not possible and their own capacity of intervention is limited, the Organisation of the American States will assume its mission by intervening in order to materialize the political stability needed to reach the way to economical progress and pacific relations in the continent.

Sistemas de saúde e a farmácia comunitária em Portugal

Projeto de Pós-Graduação/Dissertação apresentado à Universidade Fernando Pessoa como parte dos requisitos para obtenção do grau de Mestre em Ciências Farmacêuticas

Estratégias terapêuticas baseadas na modulação da atividade enzimática das caspases

Projeto de Pós-Graduação/Dissertação apresentado à Universidade Fernando Pessoa como parte dos requisitos para obtenção do grau de Mestre em Ciências Farmacêuticas

Abuso infantil

Projeto de Pós-Graduação/Dissertação apresentado à Universidade Fernando Pessoa como parte dos requisitos para obtenção do grau de Mestre em Medicina Dentária

Perceções de profissionais do Sistema de Justiça e População em geral quanto à implementação da Lei de Registo e Notificação de Ofensores Sexuais em Portugal

Dissertação apresentada à Universidade Fernando Pessoa como parte dos requisitos para obtenção do grau de mestre em Psicologia Jurídica

Monstrative acts and becoming-monster: on identity, bodies, and the feminine other

Using two examples of literary monsters, the Creature in Mary Shelley’s Frankenstein (1818), and Grendel’s Mother in Beowulf, this thesis demonstrates the bearing fictional identities have on “real” bodies, through an examination of two further literary texts, David Henry Hwang’s play, M. Butterfly (1986) and J. M. Coetzee’s novel, Disgrace (1999). Western definitions of Being have historically divided body and mind, favouring the mind as formative of subjective experience and denigrating the body as secondary and impure. This thesis demonstrates that this mind/body binary is symptomatic of the masculine ontological imperative to disown the body and its effects on Being, simultaneously ridding itself of the feminine it believes is its irrational opposite. Using recent feminist reviews of the canon, which emphasise the body’s importance to ontology and demonstrate the conceptual association between the feminine and the corporeal, this thesis links performative identity practices to theories of monstrosity, explaining how fictional qualities adhere to monstrous bodies by proposing a new theoretical category, the “monstrative.” The monstrative is a performative force that makes the Other into a living sign of Otherness; however, unlike earlier theories of Othering, the monstrative accounts for the Other’s being other to herself. This thesis also attempts to read the misrepresented body of the Other as a possible site for more empowered identity performances, where the monstrous “I” is interpreted as a potentially positive model for identity practice, through the conceptualisation of identity as a process of Becoming rather than Being. The transferal from a noun to a verb not only emphasises the performativity of identity, but also suggests fluidity and multiplicity in identity practice, which always already indicates a monstrosity at work. Thus, while monstrative acts constitute bodies as monstrous, Becoming-monster is an empathetic response to the Other’s monstrosity.

Minor Moves: Growth, Fugitivity, and Children's Physical Movement

From tendencies to reduce the Underground Railroad to the imperative "follow the north star" to the iconic images of Ruby Bridges' 1960 "step forward" on the stairs of William Frantz Elementary School, America prefers to picture freedom as an upwardly mobile development. This preoccupation with the subtractive and linear force of development makes it hard to hear the palpable steps of so many truant children marching in the Movement and renders illegible the nonlinear movements of minors in the Underground. Yet a black fugitive hugging a tree, a white boy walking alone in a field, or even pieces of a discarded raft floating downstream like remnants of child's play are constitutive gestures of the Underground's networks of care and escape. Responding to 19th-century Americanists and cultural studies scholars' important illumination of the child as central to national narratives of development and freedom, "Minor Moves" reads major literary narratives not for the child and development but for the fugitive trace of minor and growth.

In four chapters, I trace the physical gestures of Nathaniel Hawthorne's Pearl, Harriet Beecher Stowe's Topsy, Harriet Wilson's Frado, and Mark Twain's Huck against the historical backdrop of the Fugitive Slave Act and the passing of the first compulsory education bills that made truancy illegal. I ask how, within a discourse of independence that fails to imagine any serious movements in the minor, we might understand the depictions of moving children as interrupting a U.S. preoccupation with normative development and recognize in them the emergence of an alternative imaginary. To attend to the movement of the minor is to attend to what the discursive order of a development-centered imaginary deems inconsequential and what its grammar can render only as mistakes. Engaging the insights of performance studies, I regard what these narratives depict as childish missteps (Topsy's spins, Frado's climbing the roof) as dances that trouble the narrative's discursive order. At the same time, drawing upon the observations of black studies and literary theory, I take note of the pressure these "minor moves" put on the literal grammar of the text (Stowe's run-on sentences and Hawthorne's shaky subject-verb agreements). I regard these ungrammatical moves as poetic ruptures from which emerges an alternative and prior force of the imaginary at work in these narratives--a force I call "growth."

Reading these "minor moves" holds open the possibility of thinking about a generative association between blackness and childishness, one that neither supports racist ideas of biological inferiority nor mandates in the name of political uplift the subsequent repudiation of childishness. I argue that recognizing the fugitive force of growth indicated in the interplay between the conceptual and grammatical disjunctures of these minor moves opens a deeper understanding of agency and dependency that exceeds notions of arrested development and social death. For once we interrupt the desire to picture development (which is to say the desire to picture), dependency is no longer a state (of social death or arrested development) of what does not belong, but rather it is what Édouard Glissant might have called a "departure" (from "be[ing] a single being"). Topsy's hard-to-see pick-pocketing and Pearl's running amok with brown men in the market are not moves out of dependency but indeed social turns (a dance) by way of dependency. Dependent, moving and ungrammatical, the growth evidenced in these childish ruptures enables different stories about slavery, freedom, and childishness--ones that do not necessitate a repudiation of childishness in the name of freedom, but recognize in such minor moves a fugitive way out.