Extraction of added-value products from biomass using ionic liquids

The main purpose of this thesis is to investigate the potential of ionic liquids (ILs) as a new class of extractive solvents for added-value products from biomass. These include phenolic compounds (vanillin, gallic, syringic and vanillic acids), alkaloids (caffeine) and aminoacids (L-tryptophan). The interest on these natural compounds relies on the wide variety of relevant properties shown by those families and further application in the food, cosmetic and pharmaceutical industries. Aiming at developping more benign and effective extraction/purification techniques than those used, a comprehensive study was conducted using aqueous biphasic systems (ABS) composed of ILs and inorganic/organic salts. In addition, ILs were characterized by a polarity scale, using solvatochromic probes, aiming at providing prior indications on the ILs affinity for particular added-value products. Solid-liquid (S-L) extractions from biomass and using aqueous solution of ILs were also investigated. In particular, and applying and experimental factorial design to optimize the operational conditions, caffeine was extracted from guaraná seeds and spent coffee. With both types of extractions it was found that it is possible to recover the high-value compounds and to recycle the IL and salt solutions. Finally, aiming at exploring the recovery of added-value compounds from biomass using a simpler and more suistainable technique, the solubility of gallic acid, vanillin and caffeine was studied in aqueous solutions of several ILs and common salts. With the gathered results it was possible to demonstrate that ILs act as hydrotropes and that water can be used as an adequate antisolvent. This thesis describes the use of ILs towards the development of more effective and sustainable processes.

Purificação e caracterização de proteínas Gla nos modelos peixe e mamífero

Dissertação de Mestrado, Biologia Molecular e Microbiana, Faculdade de Ciências e Tecnologia, Universidade do Algarve, 2010

Cloning, expression and purification of pneumococcal proteins = clonagem, expressão e purificação de proteínas de pneumococcus

Dissertação de mest., Engenharia Biológica, Faculdade de Ciências e Tecnologia, Univ. do Algarve, 2008

Avaliação do potencial da Salicornia ramosissima para saladas frescas ou em pó (sal verde)

Dissertação de mest., Tecnologia dos Alimentos , Instituto Superior de Engenharia, Univ. do Algarve, 2013

Produção e purificação da proteína rica em gla (grp) humana recombinante

Dissertação de mest., Biologia Molecular e Microbiana, Faculdade de Ciências e Tecnologia, Univ. do Algarve, 2011

Isolamento e purificação do nortriterpeno phlomispurpentaolone a partir de raízes de Phlomis purpurea e determinação da sua atividade citotóxica nas linhas celulares HeLa e L929

Dissertação de mestrado, Ciências Farmacêuticas, Faculdade de Ciências e Tecnologia, Universidade do Algarve, 2015

Potencial da diversidade microbiana dos Açores na saúde : screening, purificação e caracterização de uma enzima trombolítica

Dissertação de Mestrado, Mestrado em Ciências Biomédicas, 8 de Maio de 2015, Universidade dos Açores.

Principios técnicos de salado y secado del pescado. Estudio químico de la sal en el litoral

Describe criterios y técnicas apropiadas para el procesamiento industrial de "bonito" salado -seco y demostrar la factibilidad de obtener un producto de óptima calidad.

Arrêt du Conseil d'Etat qui ordonne que les pourvus des offices de conseillers secrétaires du Roi, créés près les Parlements et autres cours supérieures du royaume jouiront... d'un minot de franc salé par chacun an

[Acte. 1706-06-08. Versailles]

Optimización de la sintesis del ácido xilarico, obtención y caracterización de su sal de magnesio.

Tesis (Maestría en Ciencias Especialidad en Química Orgánica) UANL