Essential factors for control of the equilibrium in the reversible rearrangement of M3N@Ih-C80 fulleropyrrolidines: Exohedral functional groups versus endohedral metal clusters

The effects of exohedral moieties and endohedral metal clusters on the isomerization of M3N@Ih-C80 products from the Prato reaction through [1,5]-sigmatropic rearrangement were systematically investigated by using three types of fulleropyrrolidine derivatives and four different endohedral metal clusters. As a result, all types of derivatives provided the same ratios of the isomers for a given trimetallic nitride template (TNT) as the thermodynamic products, thus indicating that the size of the endohedral metal clusters inside C80 was the single essential factor in determining the equilibrium between the [6,6]-isomer (kinetic product) and the [5,6]-isomer. In all the derivatives, the [6,6]- and [5,6]-Prato adducts with larger metal clusters, such as Y3N and Gd3N, were equally stable, which is in good agreement with DFT calculations. The reaction rate of the rearrangement was dependent on both the substituent of exohedral functional groups and the endohedral metal-cluster size. Further DFT calculations and 13C NMR spectroscopic studies were employed to rationalize the equilibrium in the rearrangement between the [6,6]- and [5,6]-fulleropyrrolidines

The adequacy of different robust statistical tests in comparing two independent groups

In the current study, we evaluated various robust statistical methods for comparing two independent groups. Two scenarios for simulation were generated: one of equality and another of population mean differences. In each of the scenarios, 33 experimental conditions were used as a function of sample size, standard deviation and asymmetry. For each condition, 5000 replications per group were generated. The results obtained by this study show an adequate type error I rate but not a high power for the confidence intervals. In general, for the two scenarios studied (mean population differences and not mean population differences) in the different conditions analysed, the Mann-Whitney U-test demonstrated strong performance, and a little worse the t-test of Yuen-Welch.

A direct potentiometric titration study of the dissociation of humic acid with selectively blocked functional groups

A direct potentiometric titration method was applied to commercial and soil humic acids in order to determine their carboxyl and phenol group concentrations and apparent and intrinsic pK. In that context, acid-base properties of humic acids are interpreted by selective blocking of carboxylic and phenolic groups by esterification and acetylation. Differences in underivatized and derivatized HA's acid-base properties are ascribed to carboxyl and phenol groups influence on total humic acidity. Potentiometric data were treated with the modified Henderson-Hasselbalch equation. Infra red results, the acidic group contents and the average values of apparent and intrinsic pK for underivatized and derivatized HAs confirmed the selectivity of esterification derivatization method. After blocking of the functional groups, the values of acidic group contents decreased, while the value of apparent pK increased after derivatization. Phenol groups cannot be specifically identified by the acetylation method, due to low selectivity of the acetylation method.

Evaluation of group electronegativities and hardness (softness) of group 14 elements and containing functional groups through density functional theory and correlation with NMR spectra data

Quantum Chemical calculations for group 14 elements of Periodic Table (C, Si, Ge, Sn, Pb) and their functional groups have been carried out using Density Functional Theory (DFT) based reactivity descriptors such as group electronegativities, hardness and softness. DFT calculations were performed for a large series of tetracoordinated Sn compounds of the CH3SnRR'X type, where X is a halogen and R and R' are alkyl, halogenated alkyl, alkoxy, or alkyl thio groups. The results were interpreted in terms of calculated electronegativity and hardness of the SnRR'X groups, applying a methodology previously developed by Geerlings and coworkers (J. Phys. Chem. 1993, 97, 1826). These calculations allowed to see the regularities concerning the influence of the nature of organic groups RR' and inorganic group X on electronegativities and hardness of the SnRR'X groups; in this case, it was found a very good correlation between the electronegativity of the fragment and experimental 119Sn chemical shifts, a property that sensitively reflects the change in the valence electronic structure of molecules. This work was complemented with the study of some compounds of the EX and ER types, where E= C, Si, Ge, Sn and R= CH3, H, which was performed to study the influence that the central atom has on the electronegativity and hardness of molecules, or whether these properties are mainly affected for the type of ligand bound to the central atom. All these calculations were performed using the B3PW91 functional together with the 6-311++G** basis set level for H, C, Si, Ge, F, Cl and Br atoms and the 3-21G for Sn and I atoms.

Potentiometric quantification and speciation of oxygenated groups in humic substances using BEST7 software

In this study the BEST7 software was employed to quantify different classes of functional groups and to model the proton titration behavior of humic substances. To illustrate the process, the Suwannee River fulvic acid of the IHSS (International Humic Substances Society) was used. Five categories - two classes of phenolic groups (phenol and cathecol), two classes of carboxylic groups (benzoic and phtalic) and the combination between them (salicylic) - of oxygenated groups were considered as being responsible for the potentiometric behavior of the sample and were quantitatively determined. The most and the least abundant groups were cathecol (3.300 ± 0.010 mmol g-1) and phenol (1.225 ± 0.070 mmol g-1), respectively. The estimated equilibrium constants were also determined and were in good agreement with the literature values for phenol and cathecol groups and for benzoic, phtalic and salicylic acids. Distribution diagrams of the species were generated with the software SPE and SPEPLOT.

Considering Target Groups in the Creation of a Comprehensive City Branding Process

The aim of the research was to create a comprehensive city branding process. This was done by identifying the key target groups of the city and considering them in the city branding process. Also key stakeholders were identified and taken into consideration when creating the branding process. As an empirical study, three first stages of the city branding process were implemented for the city of Lappeenranta having "students" as the case target group. An interview with the city officials was conducted, as well as a student survey on the current city image of Lappeenranta. Quantitative research methods were used to analyze the results of the survey. A comprehensive city branding process with eight stages was created in the research. Target groups were considered in the process by identifying the target group dependent stages. The empirical study revealed that the current city image held by the students consists of six dimensions. These dimensions were analyzed from the viewpoint of Lappeenranta with the help of an importance performance analysis.

Sales performance measurement and steering in a multinational company

The objective of the study is to find out how sales performance should be measured and how should sales be steered in a multinational company. The beginning of the study concentrates on the literature regarding sales, performance measurement, sales performance measurement, and sales steering. The empirical part of the study is a case study, in which the information was acquired from interviews with the key personnel of the company. The results of the interviews and the revealed problems were analyzed, and comparison for possible solutions was performed. When measuring sales performance, it is important to discover the specific needs and objectives for such a system. Specific needs should be highlighted in the design of the system. The system should be versatile and the structure of the system should be in line with the organizational structure. The role of the sales performance measurement system was seen to be important in helping sales steering. However, the importance of personal management and especially conversations were seen as really critical issue in the steering. Sales performance measurement could be based on the following perspectives: financial, market, customer, people, and future. That way the sales department could react to the environmental changes more rapidly.

Diameter structure of stratum and ecological groups of a Semideciduous Forest area in Dionísio-MG

The objective of this study was to obtain homogeneous groups of species and information on their density, dominance and volume, in terms of ecological group and diameter structure of an area of Submontane Semideciduous forest (Mata do Mumbaça) in Dionísio, MG. This work was conducted with data of the diameter distribution per species from floristic and phytosociological (Mata do Mumbaça) survey of 120 plots with 10 x 10 m each one. The 120 plots were contiguous and corresponding to a total sample area of 12,000 m² distributed over the topographic units (Low Ramp, Lower Slope, Upper Slope and Hill Top). The topographic units Low Ramp, Lower Slope and Upper Slope were in the middle stage of succession as they presented incipient stratification into two strata (canopy and understory) i.e. canopy ranging from 5 to 12 m high. However, the stratum Hill Top was classified as intermediate/advanced succession because it had a total height equal to or greater than 12 m. The distribution of individual trees of the four strata on diameter classes showed a typical J-inverted pattern that is, high concentration of individuals in smaller diameter classes and a sharp reduction towards the larger classes. In relation to absolute dominance and total volume of species, the ecological group that stood out in the four strata (Low Ramp, Lower Slope, Upper Slope and Hill Top) was the initial secondary, which were in the intermediate stage of secondary, rapidly developing into the mature phase.

Lapsen sosiaalisen kompetenssin tukeminen – vanhempien epäsuorat ja suorat vaikutustavat

Tässä tutkimuksessa tarkastellaan vanhempien havaintoja ja käsityksiä lapsen sosiaalisesta kompetenssista. Lapsen sosiaalisesta kompetenssista tarkastelun kohteena ovat erityisesti vertaissuhteet, sosiaaliset taidot ja sosiaalinen käyttäytyminen. Tarkoituksena on selvittää vanhempien näkemyksiä lapsen sosiaalisesta verkostosta ja lapsesta sosiaalisena toimijana. Kiinnostuksen kohteena on myös, miten vanhemmat vaikuttamaan lapsen sosiaaliseen kompetenssiin. Vanhempien vaikutuksessa voidaan erottaa epäsuora ja suora vaikutus. Vanhempien epäsuoraan vaikutukseen kuuluvat perheen sosioekonomiset tekijät, vanhemmuuteen ja lastenkasvatukseen liittyvät käytännöt sekä lapsen ja vanhemman välinen vuorovaikutus. Suora vaikutus sisältää vanhempien eri roolit ja tehtävät sosiaalisen kompetenssin edistämiseksi. Vanhempien epäsuorilla ja suorilla vaikutustavoilla on havaittu olevan merkittävää vaikutusta lapsen sosiaalisen kompetenssiin muotoutumiseen ja sen laatuun. Tutkimuksessa selvitetään vertaissuhdeongelmaisten ja ei-ongelmaisten lasten vanhempien välisiä eroja näissä vaikutustavoissa. Tutkimuksessa hyödynnetään kyselylomake- ja haastatteluaineistoja. Kyselylomakeaineisto (N=156) kerättiin ”Origins of Exclusion in Early Childhood”-tutkimusprojektissa, jossa tutkittiin lasten vertaissuhteita, sosiaalisia taitoja sekä sosiaalista käyttäytymistä kolmen vuoden seurantatutkimuksena päiväkodista kouluun. Perhekysely toteutettiin lasten ollessa kuusivuotiaita. Vanhempien haastatteluaineisto (N=55) koostuu projektissa mukana olleiden lasten vanhempien teemahaastatteluista. Perhekyselyä analysoidaan tilastollisin analyysimenetelmin. Laadullisen aineiston analyysimenetelmänä käytetään sisällönanalyysia. Vanhempien käsityksissä lasten sosiaalinen verkosto rakentui kotiympäristössä, koulussa, päiväkodissa sekä suvun ja harrastusten parissa muodostuneista suhteista. Tutkimustulosten perusteella on havaittavissa, että vertaissuhdeongelmaisten ja ei-ongelmaisten lasten sosiaaliset verkostot ovat osin erilaiset. Vanhempien arviointien mukaan myös lasten sosiaalisissa taidoissa, käyttäytymisessä, asennoitumisessa sosiaaliseen kanssakäymiseen sekä ryhmään ja leikkeihin liittyvissä strategioissa on eroavaisuuksia. Tutkimuksen pohjalta voidaan todeta, että vanhemmat pystyvät arvioimaan hyvin yksityiskohtaisesti lapsensa sosiaalisia taitoja ja käyttäytymistä. Kaikilla vanhemmilla ei kuitenkaan ollut riittävästi tietoa lasten kaveripiiristä tai sen laadusta eikä lasten sosiaalisesta orientaatiosta. Vanhempien epäsuorissa vaikutustavoissa oli eroja, mutta myös yhtäläisyyksiä. Sosioekonomisia tekijöitä koskevan tarkastelun perusteella vertaissuhdeongelmaisten lasten perheiden taloudelliset ongelmat, isien työttömyys ja lapsen erityisen tuen tarve olivat yhteydessä lapsen sosiaalisten suhteiden ongelmiin. Lähes kaikki tutkimukseen osallistuneet vanhemmat kokivat vanhemmuuden ja kasvatustehtävän kuitenkin hyvin myönteisenä ja tyytyväisyyttä tuottavana asiana elämässä. Valtaosa vanhemmista piti lapsen ja aikuisen välistä vuorovaikutusta positiivisena, vaikka lapsen kanssa ei aina ollutkaan helppoa tulla toimeen. Tyytyväisyydestä huolimatta äidit näkivät itsessään enemmän kehittymisen tarpeita vanhempana kuin isät. Vanhemmuudessa korostuivat ohjaaminen ja kontrolli, mutta myös hoiva, lämpö ja vastavuoroisuus. Hoiva ja lämpö sekä rajojen asettaminen askarruttivat vanhempia suuresti. Vertaissuhdeongelmaisten lasten vanhemmat tarvitsisivat opastusta ohjaavan vanhemmuuden löytämiseksi. Vertaissuhdeongelmaisten lasten vanhemmat kuvasivat kasvatuksen kuormittavuutta, ajan puutetta sekä muuntuvaa isyyttä ja äitiyttä ei-ongelmaisten lasten vanhempia enemmän. Työn ja perheen yhteensovittamisen vaikeus tuli myös esille vanhempien kuvauksissa. Kyvykkään vanhemmuuden kannalta epävirallinen läheisistä muodostunut tukiverkosto on tärkeä vanhemman apu ja kasvatuksen turva. Ensisijaisena tukitahona on epävirallinen verkosto, joka koostuu ystävistä, tuttavista, työtovereista, puolisosta ja omista vanhemmista. Vertaissuhdeongelmaisten lasten vanhempien mukaan arjen tukea ei kuitenkaan ole aina saatavilla, eikä tukiverkosto tyydyttänyt vanhempia. Vanhempien käsityksissä perheen vuorovaikutus sujui hyvin ja vastuu kodista ja kasvatustehtävästä oli molemmilla vanhemmilla tasavertaisesti. Käytännön vastuu kasvatuksesta sekä erilaisten taitojen opettamisesta lapsille kuului äitien tehtäviin. Vanhempien näkemyksissä lapsen sosiaalinen maailma rakentui lähiympäristön tarjoamista mahdollisuuksista. Vanhempien suoriin vaikutustapoihin liittyvien tulosten mukaan vanhemmat pitävät harrastuksia merkittävänä sosiaalista kompetenssia edistävänä tekijänä. Ei-ongelmaisilla lapsilla oli enemmän ja monipuolisempia harrastuksia kuin ongelmaisilla lapsilla. Vaikka vanhemmat eivät mieltäneetkään omaa toimintaansa kaveripiiriin ohjaamiseksi, heillä useinkin oli runsaasti erilaisia rooleja ja tehtäviä sosiaalisten suhteiden ja sosiaalisten taitojen opettamisessa. Lapsen sosiaalisten suhteiden organisoinnissa, ohjaamisessa, valvonnassa ja neuvonnassa oli vanhempiryhmien välillä eroja. Vertaissuhdeongelmaisten lasten vanhemmat eivät olleet riittävän hyvin perillä lapsen taidoista ja kyvykkyydestä tuottaakseen oikea-aikaista ohjausta ja tukeakseen lasta riittävästi sopivilla tavoilla. Vanhempien toimintaa näyttää ohjaavan vakaasti se uskomus, että lapsen kaverisuhteet ovat pelkästään hänen oma asiansa. Vanhemmat arvostivat paljon sosiaalisia taitoja ja pyrkivät opettamaan niitä lapsilleen systemaattisesti. Ohjaamisen tavoissa vanhemmat poikkesivat toisistaan. Tutkimus antaa viitteitä, että eiongelmaisten lasten vanhemmat ovat sensitiivisempiä, vastavuoroisempia ja lapsen näkökulmaa ja tarpeita lähtökohtanaan pitäviä sekä tunnetaitoihin ja tunnetilan käsittelyyn keskittyvämpiä kuin ongelmaisten lasten vanhemmat. Vanhempien ja lasten vuorovaikutuksen vaikeudet, ylimalkainen ja epäjohdonmukainen sosiaalisten taitojen, käyttäytymisen sekä suhteiden ohjaus sekä monet perheen voimavarojen puutteet voivat haitata vakavasti lapsen sosiaalisen kompetenssin kehittymistä. Vanhemmilla on kuitenkin taitoa arvioida lapsensa sosiaalista kyvykkyyttä ja halua toimia hyvin kasvatustehtävässään. Vanhemmuuden tukemisessa olisivatkin tärkeitä epävirallisen tukiverkoston lisäksi lähiympäristön perheille ja lapsille suunnatut palvelut, monitahoinen yhteistyö perheiden, lasten ja heidän kanssaan työskentelevien ammattilaisten kesken sekä yhteiskunnan perheitä koskevat säädökset ja tukitoimet.

The role of business information and knowledge in sales steering

The objective of this study is to find out how sales management can be optimally supported with business information and knowledge. The first chapters of the study focus on theoretical literature about sales planning, sales steering, business intelligence, and knowledge management. The empirical part of the study is a case study for which the material was collected through interviews with the selected people of the company. The findings from the interviews were analyzed, and possible suggestions for solving the problems were made. The case study revealed that sales management requires a multitude of metrics and reports to steer the sales to the desired direction. The information sources can be internal and external, and the optimal solution for satisfying the information needs is a combination of both of these. The simple information should be turned into knowledge by merging the intellectual assets with the information from the firm’s transaction processing systems, in order to promote organizational learning and effective decision-making.

Collaborative information behaviour : a case study of two research groups

The aim of the licentiate thesis is to examine researchers' information practices in research groups. The researchers were involved with study communication and media related issues within Social Sciences and Humanities Faculties. The theoretical framework of the study comprises the new holistic models of information seeking (for example: Meho and Tibbo, 2003; Seldén, 1999) and the collective aspects of information behaviour (Prekop, 2002 ; Talja, 2002; Talja and Hansen, 2006). The research questions are: 1. How do scholars seek information in research groups? 2 What kind of collaborative information behaviour occurs in the research groups? The research data was gathered by interviews and observations. Three meetings of a research group at the University of Tampere were observed during the autumn of 2004. The group members and the group leader of the research group were interviewed in the spring of 2005. The research group members and the group leader of a research group at the University of Jyväskylä were interviewed in the autumn of 2005. Altogether, two research group leaders and eight researchers were interviewed. The significance of the research group for information seeking is more important in closeknit research groups than in rather loose research groups. The significance of the research group for information seeking can be at least threefold. First, research group members can inform the group about relevant information resources and potential library or other information services. Second, the research group can to some extent compensate for the information seeking systems of libraries by distributing material and information resources. Third, information seeking can be carried out in collaboration in research groups. The significance of the research group was found to be most important in informing about new information services and marketing library systems. Recommendations from colleagues were often needed to mobilize researchers into using new library services. The significance of colleagues in informing about library services is in line with earlier studies. The present study showed that sometimes information from colleagues was regarded as more important than information distributed directly by the local library. A culture of information sharing, including mutual trust, seemed mainly to be reflected in collaboration and collaborative information seeking in the research groups studied. The timing of the onset of individual research seemed to be related to the information sharing culture and social networks in research groups. The simultaneous onset of the research work by group members seemed to promote the growth of unbiased collaboration, also in information seeking.

Diarrhea outbreaks in suckling piglets due to rotavirus group C single and mixed (rotavirus groups A and B) infections

Porcine group A rotavirus (PoRVA) is a major cause of neonatal diarrhea in suckling and recently weaned piglets worldwide. The involvement of non-group A rotavirus in cases of neonatal diarrhea in piglets are sporadic. In Brazil there are no reports of the porcine rotavirus group C (PoRVC) as etiologic agent of the diarrhea outbreaks in piglets. The aim of this study was to describe the identification of rotavirus group C in single and in mixed infection with rotavirus groups A and B in three neonatal diarrhea outbreaks in suckling (<21-day-old) piglets, with 70% to 80% and 20% to 25% of morbidity and lethality rates, respectively, in three pig herds located in the state of Santa Catarina, Brazil. The diagnosis of PoRV in the diarrheic fecal samples was performed using polyacrylamide gel electrophoresis (PAGE) to identify the presence of porcine rotavirus groups A, B (PoRVB), and C, and by RT-PCR (PoRVA and PoRVC) and semi-nested (SN)-PCR (PoRVB) to partially amplify the VP4 (VP8*)-VP7, NSP2, and VP6 genes of PoRVA, PoRVB, and PoRVC, respectively. One RT-PCR (PoRVA and PoRVC) and SN-PCR (PoRVB) product of each group of rotavirus of each diarrhea outbreak was submitted to nucleotide (nt) sequence analysis. Based on the PAGE technique, 4 (25%) and 1 (6.25%) of the 16 diarrheic fecal samples evaluated in the first outbreak presented PoRVA and PoRVC electropherotype, respectively, and 11 (68.75%) were negative. In the second outbreak, 3 (42.85%) of the 7 fecal samples evaluated presented PoRVA electropherotype, and in 3 (42.85%) and in 1 (14.3%) fecal samples were detected inconclusive and negative results, respectively. Three (30%) of the 10 fecal samples of the third outbreak presented PoRVC electropherotype; 5 (50%) and 2 (20%) samples showed negative and inconclusive results, respectively. Based on the RT-PCR and SN-PCR assays in the first neonatal diarrhea outbreak, PoRVC was detected in 13 (81.2%) of the 16 diarrheic fecal samples evaluated. PoRVC single infection was identified in 4 (25%) of these samples and mixed infections with PoRVA and PoRVB in 9 (56.2%) fecal samples. All of the seven diarrheic fecal samples evaluated from the second neonatal diarrhea outbreak were positive for PoRVC, whereas its mixed infection with other PoRV groups was detected in 4 (57.2%) samples. In the third outbreak, PoRVC in single infection was detected in all of the 10 diarrheic fecal samples analyzed. In the nt sequence analysis, the PoRVA strains of the first and second outbreaks demonstrated higher nt identity with G4P[6] and G9P[23] genotypes, respectively. The PoRVB strains (first and second outbreaks) and the PoRVC strains (first, second, and third outbreaks) showed higher nt identity and clustered in the phylogenetic tree with PoRVB and PoRVC strains that belong to the N4 and I1 genotypes, respectively. This is the first description in Brazil of the involvement of PoRVC in the etiology of diarrhea outbreaks in suckling piglets. The results of this study demonstrated that PoRVC, in both single and mixed infections, is an important enteropathogen involved in neonatal diarrhea outbreaks in piglets and that the use of more sensitive diagnostic techniques allows the identification of mixed infections involving two or even three groups of PoRV, which may be more common than previously reported.

Network connectance and autonomy analyses of the photosynthetic apparatus in tropical tree species from different successional groups under contrasting irradiance conditions

Biological systems are complex dynamical systems whose relationships with environment have strong implications on their regulation and survival. From the interactions between plant and environment can emerge a quite complex network of plant responses rarely observed through classical analytical approaches. The objective of this current study was to test the hypothesis that photosynthetic responses of different tree species to increasing irradiance are related to changes in network connectances of gas exchange and photochemical apparatus, and alterations in plant autonomy in relation to the environment. The heat dissipative capacity through daily changes in leaf temperature was also evaluated. It indicated that the early successional species (Citharexylum myrianthum Cham. and Rhamnidium elaeocarpum Reiss.) were more efficient as dissipative structures than the late successional one (Cariniana legalis (Mart.) Kuntze), suggesting that the parameter deltaT (T ºCair - T ºCleaf) could be a simple tool in order to help the classification of successional classes of tropical trees. Our results indicated a pattern of network responses and autonomy changes under high irradiance. Considering the maintenance of daily CO2 assimilation, the tolerant species (C. myrianthum and R. elaeocarpum) to high irradiance trended to maintain stable the level of gas exchange network connectance and to increase the autonomy in relation to the environment. On the other hand, the late successional species (C. legalis) trended to lose autonomy, decreasing the network connectance of gas exchange. All species showed lower autonomy and higher network connectance of the photochemical apparatus under high irradiance.

Photosynthetic responses of tropical tree species from different successional groups under contrasting irradiance conditions

This study evaluated the photosynthetic responses of seven tropical trees of different successional groups under contrasting irradiance conditions, taking into account changes in gas exchange and chlorophyll a fluorescence. Although early successional species have shown higher values of CO2 assimilation (A) and transpiration (E), there was not a defined pattern of the daily gas exchange responses to high irradiance (FSL) among evaluated species. Cariniana legalis (Mart.) Kuntze (late secondary) and Astronium graveolens Jacq. (early secondary) exhibited larger reductions in daily-integrated CO2 assimilation (DIA) when transferred from medium light (ML) to FSL. On the other hand, the pioneer species Guazuma ulmifolia Lam. had significant DIA increase when exposed to FSL. The pioneers Croton spp. trended to show a DIA decrease around 19%, while Cytharexyllum myrianthum Cham. (pioneer) and Rhamnidium elaeocarpum Reiss. (early secondary) trended to increase DIA when transferred to FSL. Under this condition, all species showed dynamic photoinhibition, except for C. legalis that presented chronic photoinhibition of photosynthesis. Considering daily photosynthetic processes, our results supported the hypothesis of more flexible responses of early successional species (pioneer and early secondary species). The principal component analysis indicated that the photochemical parameters effective quantum efficiency of photosystem II and apparent electron transport rate were more suitable to separate the successional groups under ML condition, whereas A and E play a major role to this task under FSL condition.