Light scattering in random media with wavelength-scale structures : astronomical and industrial applications

Light scattering, or scattering and absorption of electromagnetic waves, is an important tool in all remote-sensing observations. In astronomy, the light scattered or absorbed by a distant object can be the only source of information. In Solar-system studies, the light-scattering methods are employed when interpreting observations of atmosphereless bodies such as asteroids, atmospheres of planets, and cometary or interplanetary dust. Our Earth is constantly monitored from artificial satellites at different wavelengths. With remote sensing of Earth the light-scattering methods are not the only source of information: there is always the possibility to make in situ measurements. The satellite-based remote sensing is, however, superior in the sense of speed and coverage if only the scattered signal can be reliably interpreted. The optical properties of many industrial products play a key role in their quality. Especially for products such as paint and paper, the ability to obscure the background and to reflect light is of utmost importance. High-grade papers are evaluated based on their brightness, opacity, color, and gloss. In product development, there is a need for computer-based simulation methods that could predict the optical properties and, therefore, could be used in optimizing the quality while reducing the material costs. With paper, for instance, pilot experiments with an actual paper machine can be very time- and resource-consuming. The light-scattering methods presented in this thesis solve rigorously the interaction of light and material with wavelength-scale structures. These methods are computationally demanding, thus the speed and accuracy of the methods play a key role. Different implementations of the discrete-dipole approximation are compared in the thesis and the results provide practical guidelines in choosing a suitable code. In addition, a novel method is presented for the numerical computations of orientation-averaged light-scattering properties of a particle, and the method is compared against existing techniques. Simulation of light scattering for various targets and the possible problems arising from the finite size of the model target are discussed in the thesis. Scattering by single particles and small clusters is considered, as well as scattering in particulate media, and scattering in continuous media with porosity or surface roughness. Various techniques for modeling the scattering media are presented and the results are applied to optimizing the structure of paper. However, the same methods can be applied in light-scattering studies of Solar-system regoliths or cometary dust, or in any remote-sensing problem involving light scattering in random media with wavelength-scale structures.

Combination antiretroviral therapy -associated lipodystrophy : insights into pathogenesis and treatment

Introduction: Combination antiretroviral therapy (cART) has decreased morbidity and mortality of individuals infected with human immunodeficiency virus type 1 (HIV-1). Its use, however, is associated with adverse effects which increase the patients risk of conditions such as diabetes and coronary heart disease. Perhaps the most stigmatizing side effect is lipodystrophy, i.e., the loss of subcutaneous adipose tissue (SAT) in the face, limbs and trunk while fat accumulates intra-abdominally and dorsocervically. The pathogenesis of cART-associated lipodystrophy is obscure. Nucleoside reverse transcriptase inhibitors (NRTI) have been implicated to cause lipoatrophy via mitochondrial toxicity. There is no known effective treatment for cART-associated lipodystrophy during unchanged antiretroviral regimen in humans, but in vitro data have shown uridine to abrogate NRTI-induced toxicity in adipocytes. Aims: To investigate whether i) cART or lipodystrophy associated with its use affect arterial stiffness; ii) lipoatrophic SAT is inflamed compared to non-lipoatrophic SAT; iii) abdominal SAT from patients with compared to those without cART-associated lipoatrophy differs with respect to mitochondrial DNA (mtDNA) content, adipose tissue inflammation and gene expression, and if NRTIs stavudine and zidovudine are associated with different degree of changes; iv) lipoatrophic abdominal SAT differs from preserved dorsocervical SAT with respect to mtDNA content, adipose tissue inflammation and gene expression in patients with cART-associated lipodystrophy and v) whether uridine can revert lipoatrophy and the associated metabolic disturbances in patients on stavudine or zidovudine based cART. Subjects and methods: 64 cART-treated patients with (n=45) and without lipodystrophy/-atrophy (n=19) were compared cross-sectionally. A marker of arterial stiffness, heart rate corrected augmentation index (AgIHR), was measured by pulse wave analysis. Body composition was measured by magnetic resonance imaging and dual-energy X-ray absorptiometry, and liver fat content by proton magnetic resonance spectroscopy. Gene expression and mtDNA content in SAT were assessed by real-time polymerase chain reaction and microarray. Adipose tissue composition and inflammation were assessed by histology and immunohistochemistry. Dorsocervical and abdominal SAT were studied. The efficacy and safety of uridine for the treatment of cART-associated lipoatrophy were evaluated in a randomized, double-blind, placebo-controlled 3-month trial in 20 lipoatrophic cART-treated patients. Results: Duration of antiretroviral treatment and cumulative exposure to NRTIs and protease inhibitors, but not the presence of cART-associated lipodystrophy, predicted AgIHR independent of age and blood pressure. Gene expression of inflammatory markers was increased in SAT of lipodystrophic as compared to non-lipodystrophic patients. Expression of genes involved in adipogenesis, triglyceride synthesis and glucose disposal was lower and of those involved in mitochondrial biogenesis, apoptosis and oxidative stress higher in SAT of patients with than without cART-associated lipoatrophy. Most changes were more pronounced in stavudine-treated than in zidovudine-treated individuals. Lipoatrophic SAT had lower mtDNA than SAT of non-lipoatrophic patients. Expression of inflammatory genes was lower in dorsocervical than in abdominal SAT. Neither depot had characteristics of brown adipose tissue. Despite being spared from lipoatrophy, dorsocervical SAT of lipodystrophic patients had lower mtDNA than the phenotypically similar corresponding depot of non-lipodystrophic patients. The greatest difference in gene expression between dorsocervical and abdominal SAT, irrespective of lipodystrophy status, was in expression of homeobox genes that regulate transcription and regionalization of organs during embryonal development. Uridine increased limb fat and its proportion of total fat, but had no effect on liver fat content and markers of insulin resistance. Conclusions: Long-term cART is associated with increased arterial stiffness and, thus, with higher cardiovascular risk. Lipoatrophic abdominal SAT is characterized by inflammation, apoptosis and mtDNA depletion. As mtDNA is depleted even in non-lipoatrophic dorsocervical SAT, lipoatrophy is unlikely to be caused directly by mtDNA depletion. Preserved dorsocervical SAT of patients with cART-associated lipodystrophy is less inflamed than their lipoatrophic abdominal SAT, and does not resemble brown adipose tissue. The greatest difference in gene expression between dorsocervical and abdominal SAT is in expression of transcriptional regulators, homeobox genes, which might explain the differential susceptibility of these adipose tissue depots to cART-induced toxicity. Uridine is able to increase peripheral SAT in lipoatrophic patients during unchanged cART.

Emergenssi tieteessä ja teologiassa : Huomioita Philip Claytonin emergenssiteoriasta

Tässä työssä tarkastellaan emergenssiä luonnontieteellisenä, metafyysisenä ja teologisena käsitteenä, ja erityisesti Philip Claytonin emergenssiteoriaa. Tutkimuskohteena on Philip Claytonin kirja Mind and Emergence From Quantum to Consciousness ja tutkimusmetodina käsitteellinen analyysi. Kun emergenssiä tarkastellaan metafyysisenä teoriana, se on mahdollista nähdä fysikalismille ja dualismille vaihtoehtoisena filosofisena katsantokantana todellisuuteen. Fysikalismi on havaittavaa todellisuutta koskeva näkemys, jonka mukaan kaikki ilmiöt ovat lopulta palautettavissa fysiikkaan ja selitettävissä fysiikan käsittein. Dualismi taas on näkemys, että erityisesti ihmisen mieli ja tietoisuus ovat jotain fysikaaliseen maailmaan nähden täysin erilaista ja siitä riippumatonta. Emergenssiteoriassa maailman nähdään kehittyvänä kokonaisuutena, jossa kompleksisuuden kasvun myötä syntyy jatkuvasti uusia ominaisuuksia. Nämä ominaisuudet eivät ole palautettavissa niihin rakenteisiin, joiden pohjalta ne ovat syntyneet. Niiden syntymistä ei ole mahdollista luonnontieteellisin tai muinkaan menetelmin ennakoida. Emergenssin heikossa muodossa tämä ennakoimattomuus on vain episteeminen, tiedon mahdollisuuksiin liittyvä rajoitus, ja kausaalisia vaikutuksia esiintyy ainoastaan fysikaalisten objektien tasolla. Metafyysinen teoria edellyttää kuitenkin vahvaa emergenssiä, jossa syntyvillä ominaisuuksilla on fysikaalisesta perustastaan riippumatonta kausaalista vaikutusta. Tätä kutsutaan alaspäin -kausaalisuudeksi. Käsitys todellisuudesta on monistinen ja holistinen. Kaikki olemassa oleva on kehittynyt samasta aineksesta, mutta ei ole palautettavissa siihen. Maailmassa on ontologisesti toisistaan poikkeavia todellisuuden tasoja. Todellisuus on enemmän kuin osiensa summa. Emergenssille on tarjottu myös teologisia sovelluksia. Kun fysikalismissa uskonnolliselle uskolle ei jää tilaa ja dualismi on luonnontieteen näkökulmasta ongelmallinen, emergenssistä on etsitty filosofista viitekehystä, jossa luonnontiede ja uskonto olisivat sovitettavissa yhteen. Ensimmäisessä luvussa käsitellään fysikalismia ja dualismia ja erityisesti filosofista ja luonnontieteellistä kritiikkiä, jota niitä vastaan voidaan esittää. Toisessa luvussa tarkastellaan erilaisia mahdollisuuksia määritellä emergenssin käsite ja esitellään erityisesti Philip Claytonin käsityksiä heikosta ja vahvasta emergenssistä, emergenssistä luonnontieteissä sekä ihmismielestä fysikaaliseen maailmaan nähden emergenttinä ilmiönä. Emergenssi mainitaan monien sellaisten ilmiöiden yhteydessä, joita on pyritty tutkimaan kaaosteorian ja kompleksisuuden tutkimuksen keinoin. Työn kolmannessa luvussa pyritään antamaan kuva emergenssin suhteesta näihin teorioihin ja ylipäätään moderniin fysiikkaan. Philip Clayton on luonut emergenssiteorian pohjalta myös teologisen teorian, jossa käsitys Jumalasta luojana ja Jumalan vaikutus maailmassa pyritään sovittamaan yhteen emergentin todellisuuskäsityksen kanssa. Tätä teologista teoriaa esitellään ja arvioidaan työn neljännessä luvussa.

Information Technology Development Needs in Community Pharmacies : A Strategic Approach

In the context of health care, information technology (IT) has an important role in the operational infrastructure, ranging from business management to patient care. An essential part of the system is medication management in inpatient and outpatient care. Community pharmacists strategy has been to extend practice responsibilities beyond dispensing towards patient care services. Few studies have evaluated the strategic development of IT systems to support this vision. The objectives of this study were to assess and compare independent Finnish community pharmacy owners and staff pharmacists priorities concerning the content and structure of the next generation of community pharmacy IT systems, to explore international experts visions and strategic views on IT development needs in relation to services provided in community pharmacies, to identify IT innovations facilitating patient care services and to evaluate their development and implementation processes, and to assess community pharmacists readiness to adopt innovations. This study applied both qualitative and quantitative methods. A qualitative personal interview of 14 experts in community pharmacy services and related IT from eight countries and a national survey of Finnish community pharmacy owners (mail survey, response rate 53%, n=308), and of a representative sample of staff pharmacists (online survey, response rate 22%, n=373) were conducted. Finnish independent community pharmacy owners gave priority to logistical functions but also to those related to medication information and patient care. The managers and staff pharmacists have different views of the importance of IT features, reflecting their different professional duties in the community pharmacy. This indicates the need for involving different occupation groups in planning the new IT systems for community pharmacies. A majority of the international experts shared the vision of community pharmacy adopting a patient care orientation; supported by IT-based documentation, new technological solutions, access to information, and shared patient data. Community pharmacy IT innovations were rare, which is paradoxical because owners and staff pharmacists perception of their innovativeness was seen as being high. Community pharmacy IT systems development processes usually had not undergone systematic needs assessment research beforehand or evaluation after the implementation and were most often coordinated by national governments without subsequent commercialization. Specifically, community pharmacy IT developments lack research, organization, leadership and user involvement in the process. Those responsible for IT development in the community pharmacy sector should create long-term IT development strategies that are in line with community pharmacy service development strategies. This could provide systematic guidance for future projects to ensure that potential innovations are based on a sufficient understanding of pharmacy practice problems that they are intended to solve, and to encourage strong leadership in research, development of innovations so that community pharmacists potential innovativeness is used, and that professional needs and strategic priorities will be considered even if the development process is led by those outside the profession.

Phytophthora in Finnish nurseries

Human-mediated movement of plants and plant products is now generally accepted to be the primary mode of introduction of plant pathogens. Species of the genus Phytophthora are commonly spread in this way and have caused severe epidemics in silviculture, horticulture as well as natural systems all over the world. The aims of the study were to gather information on the occurrence of Phytophthora spp. in Finnish nurseries, to produce information for risk assessments for these Phytophthora spp. by determining their host ranges and tolerance of cold temperatures, and to establish molecular means for their detection. Phytophthora cactorum was found to persist in natural waterbodies and results suggest that irrigation water might be a source of inoculum in nurseries. In addition to P. cactorum, isolates from ornamental nursery Rhododendron yielded three species new to Finland: P. ramorum, P. plurivora and P. pini. The only species with quarantine status, P. ramorum, was most adapted to growth in cold temperatures and able to persist in the nursery in spite of an annual sanitation protocol. Phytophthora plurivora and the closely related P. pini had more hosts among Nordic tree and plant species than P. ramorum and P. cactorum, and also had higher infectivity rates. All four species survived two weeks in -5 °C , and thus soil survival of these Phytophthoras in Finland is likely under current climatic conditions. The most common tree species in Finnish nurseries, Picea abies, was highly susceptible to P. plurivora and P. pini in pathogenicity trials. In a histological examination of P. plurivora in P. abies shoot tissues, fast necrotrophic growth was observed in nearly all tissues. The production of propagules in P. abies shoot tissue was only weakly indicated. In this study, a PCR DGGE technique was developed for simultaneous detection and identification of Phytophthora spp. It reliably detected Phytophthora in plant tissues and could discriminate most test species as well as indicate instances of multiple-species infections. It proved to be a useful detection and identification tool either applied alone or in concert with traditional isolation culture techniques. All of the introduced species of Phytophthora had properties that promote a high risk of establishment and spread in Finland. It is probable that more pathogens of this genus will be introduced and become established in Finland and other Nordic countries unless efficient phytosanitary control becomes standard practice in the international plant trade.

The effect of structure on the dilute solution properties of branched polysaccharides studied with SEC and AsFlFFF

Cereal arabinoxylans, guar galactomannans, and dextrans produced by lactic acid bacteria(LAB) are a structurally diverse group of branched polysaccharides with nutritional and industrial functions. In this thesis, the effect of the chemical structure on the dilute solution properties of these polysaccharides was investigated using size-exclusion chromatography(SEC) and asymmetric flow field-flow fractionation (AsFlFFF) with multiple-detection. The chemical structures of arabinoxylans were determined, whereas galactomannan and dextran structures were studied in previous investigations. Characterization of arabinoxylans revealed differences in the chemical structures of cereal arabinoxylans. Although arabinoxylans from wheat, rye, and barley fiber contained similar amounts of arabinose side units, the substitution pattern of arabinoxylans from different cereals varied. Arabinoxylans from barley husks and commercial low-viscosity wheat arabinoxylan contained a lower number of arabinose side units. Structurally different dextrans were obtained from different LAB. The structural effects on the solution properties could be studied in detail by modifying pure wheat and rye arabinoxylans and guar galactomannan with specific enzymes. The solution characterization of arabinoxylans, enzymatically modified galactomannans, and dextrans revealed the presence of aggregates in aqueous polysaccharide solutions. In the case of arabinoxylans and dextrans, the comparison of molar mass data from aqueous and organic SEC analyses was essential in confirming aggregation, which could not be observed only from the peak or molar mass distribution shapes obtained with aqueous SEC. The AsFlFFF analyses gave further evidence of aggregation. Comparison of molar mass and intrinsic viscosity data of unmodified and partially debranched guar galactomannan, on the other hand, revealed the aggregation of native galactomannan. The arabinoxylan and galactomannan samples with low or enzymatically extensively decreased side unit content behaved similarly in aqueous solution: lower molar mass samples stayed in solution but formed large aggregates, whereas the water solubility of the higher-molar-mass samples decreased significantly. Due to the restricted solubility of galactomannans in organic solvents, only aqueous galactomannan solutions were studied. The SEC and AsFlFFF results differed for the wheat arabinoxylan and dextran samples. Column matrix effects and possible differences in the separation parameters are discussed, and a problem related to the non-established relationship between the separation parameters of the two separation techniques is highlighted. This thesis shows that complementary approaches in the solution characterization of chemically heterogeneous polysaccharides are needed to comprehensively investigate macromolecular behavior in solution. These results may also be valuable when characterizing other branched polysaccharides.

Aluekehittämisverkostojen arviointi : Verkostotoimijanäkökulma MAL- ja hyvinvointiverkostoihin aluekehittämisen välineinä

The Finnish regional development system has gone through structural reforms from state centered governed system to multi-actor governance, based on negotiation and cooperation. One of the reforms has been the regional cohesion and competitiveness program (COCO) established in 2010. The aim of the program is to increase competitiveness through all the regions and balance the regional development by supporting networking. The main focus of the program is to improve the methods and tools for regional development. In the program there are seven thematic networks founded around topics seen important national wide. This thesis explores regional development networks and their evaluation COCO:s two thematic networks, Wellbeing and Land use, housing and transportation as examples. The aim of the thesis is to explore the network actors understanding of thematic networks as tools for regional development. In particular, the study focuses on how the actors see the possible network level outcomes and wider effects of the networking activity. In addition, the central themes of the study are the prerequisite for successful network processes and improvement of the network process effectiveness by evaluation. The research material in this study consist the interviews of the network coordinators and national and regional actors participating in the network activities. The interviews were conducted in spring 2011. Based on the research results, the networks act on national regional and network level and across them. The cooperation is based on official and unofficial relations. The structure of the networks seemed to be self-organizing and controlled at the same time. The structural elements were found to set the framework for the network process and evaluation. According to the results, the networks enabled the more effective operation of the national development system, support of the regions and political lobbying. For the regions the networks offered support for areal development, new resources and possibility to influence national discourse. As conclusion, the role of the network was to disseminate information, create possibilities for collaboration and execute projects and studies and to effect on national policy making. These factors determined the effectiveness of the networks. However, because different regions were satisfied with different network level outcomes, the utilization of the networks in the regions should be evaluated by their own objectives. Resources, motivation, competence and perceptions of the effects were found to affect the successful implementation of the network process and cooperation in networks. Some network level obstacles could be overcome with coordination, but the challenge was the ability and motivation of the areas to utilize the networks as resources and see them as part of the comprehensive development agenda. Thus, the development should focus on how to increase awareness on how to improve regional cooperation processes and how multiple regional actors could enhance their working by utilizing the networks.

Characterization and evaluation of melibiose as novel excipient in tablet compaction

Lactose is probably the most used tablet excipient in the field of pharmacy. Although lactose is thoroughly characterized and available in many different forms there is a need to find a replacer for lactose as a filler/binder in tablet formulations because it has some downsides. Melibiose is a relatively unknown disaccharide that has not been thoroughly characterized and not previously used as an excipient in tablets. Structurally melibiose is close to lactose as it is also formed from the same two monosaccharides, glucose and galactose. Aim of this research is to characterize and to study physicochemical properties of melibiose. Also the potential of melibiose to be used as pharmaceutical tablet excipient, even as a substitute for lactose is evaluated. Current knowledge about fundamentals of tableting and methods for determinating of deformation behavior and tabletability are reviewed. In this research Raman spectroscopy, X-ray powder diffraction (XRPD), near-infrared spectroscopy (NIR) and Fourier-transform infrared spectroscopy (FT-IR) were used to study differences between two melibiose batches purchased from two suppliers. In NIR and FT-IR measurements no difference between materials could be observed. XPRD and Raman however found differences between the two melibiose batches. Also the effects of moisture content and heating to material properties were studied and moisture content of materials seems to cause some differences. Thermal analytical methods, differential scanning calorimetry (DSC) and thermogravimetry (TG) were used to study thermal behaviour of melibiose and difference between materials was found. Other melibiose batch contains residual water which evaporates at higher temperatures causing the differences in thermal behaviour. Scanning electron microscopy images were used to evaluate particle size, particle shape and morphology. Bulk, tapped and true densities and flow properties of melibiose was measured. Particle size of the melibiose batches are quite different resulting causing differences in the flowability. Instrumented tableting machine and compression simulator were used to evaluate tableting properties of melbiose compared to α-lactose monohydrate. Heckel analysis and strain-rate sensitivity index were used to determine deformation mechanism of melibiose monohydrate in relation to α–lactose monohydrate during compaction. Melibiose seems to have similar deformation behaviour than α-lactose monohydrate. Melibiose is most likely fragmenting material. Melibiose has better compactibility than α – lactose monohydrate as it produces tablets with higher tensile strength with similar compression pressures. More compression studies are however needed to confirm these results because limitations of this study.

The potential of microbial ecological indicators to guide ecosophisticated management of hydrocarbon-contaminated soils

Soil is an unrenewable natural resource under increasing anthropogenic pressure. One of the main threats to soils, compromising their ability to provide us with the goods and ecosystem services we expect, is pollution. Oil hydrocarbons are the most common soil contaminants, and they disturb not just the biota but also the physicochemical properties of soils. Indigenous soil micro-organisms respond rapidly to changes in the soil ecosystem, and are chronically in direct contact with the hydrophobic pollutants on the soil surfaces. Soil microbial variables could thus serve as an intrinsically relevant indicator of soil quality, to be used in the ecological risk assessment of contaminated and remediated soils. Two contrasting studies were designed to investigate soil microbial ecological responses to hydrocarbons, together with parallel changes in soil physicochemical and ecotoxicological properties. The aim was to identify quantitative or qualitative microbiological variables that would be practicable and broadly applicable for the assessment of the quality and restoration of oil-polluted soil. Soil bacteria commonly react on hydrocarbons as a beneficial substrate, which lead to a positive response in the classical microbiological soil quality indicators; negative impacts were accurately reflected only after severe contamination. Hydrocarbon contaminants become less bioavailable due to weathering processes, and their potentially toxic effects decrease faster than the total concentration. Indigenous hydrocarbon degrader bacteria, naturally present in any terrestrial environment, use specific mechanisms to improve access to the hydrocarbon molecules adsorbed on soil surfaces. Thus when contaminants are unavailable even to the specialised degraders, they should pose no hazard to other biota either. Change in the ratio of hydrocarbon degrader numbers to total microbes was detected to predictably indicate pollutant effects and bioavailability. Also bacterial diversity, a qualitative community characteristic, decreased as a response to hydrocarbons. Stabilisation of community evenness, and community structure that reflected clean reference soil, indicated community recovery. If long-term temporal monitoring is difficult and appropriate clean reference soil unavailable, such comparison could possibly be based on DNA-based community analysis, reflecting past+present, and RNA-based community analysis, showing exclusively present conditions. Microbial ecological indicators cannot replace chemical oil analyses, but they are theoretically relevant and operationally practicable additional tools for ecological risk assessment. As such, they can guide ecologically informed and sustainable ecosophisticated management of oil-contaminated lands.

Jos olisin viisas niin siivoisin : Lasten luovat prosessit päiväkodissa

It has been found usually to talk in the early childhood education in connection with the creativity about arts and skills and about play. In this treatise, the creativity is approached besides play but also from the point of view of the creativity of the everyday. The starting point for the study is the view according to which the creativity is complex interaction between a creative person and an environment. The theoretical body of the study is the Componential theory of creativity of Amabile (1996). The process which is open and product which is new and suitable or acceptable were defined creative. In the opinion of many researchers, the creativity is a phenomenon that has determined in a certain time and place so the creativity is examined from the point of view of the social constructionism. As creative processes in the day nursery it has been defined pretend play, child´s involvement and children´s agentive perception which is based on the Children´s agentive perception theory of Reunamo (2007). The purpose of the study is to clarify how the child's personal factors and the social environment affect the creative processes of children in the day nursery. This Master's thesis is based on the Children' s agentive perception uncovered study led by Jyrki Reunamo (2010) which was carried out in the spring of 2010 in Keski-Uusimaa and in Hämeenlinna and Taiwan. From the study, a name has also been used "on the sources of Orientation", a research project and development project. The study includes the children's evaluation sector, the observation sector, the children's interview sector and the evaluation sector of the pedagogic environment. 891 Children 1-7 year-old by age participated in the study. All the sectors which belong to the study of Reunamo were utilized in this treatise and the Finnish day nurseries or preschool groups which had participated in the study were marked off as the target group. The main component analysis, sum variables, the correlation coefficients, Mann-Whitney s U-test and Kruskall-Wallas test were used for the statistical examination of the quantitative material. In this treatise it was noticed, both the personal properties of the child and a social environment, that they affected all the examined creative processes which also had a significant connection with each other statistically. The definition of creativity was filled best by the participative answers. However, the number of the participative answers was only 8% in the questions concerning adults. That raised the question whether an attempt should be made to have effect so that the children's better participation also in the interaction with the adults would be possible in the educational culture of the day nursery. In the further study, the conscious building of the social environment which supports the creativity from a social constructionism point of view could indeed be an interesting task. The treatise is suitable for an examination of the interaction between the child's person and a social environment especially from the point of view of the creativity.

Taxonomy and phylogeny of the manna lichens and allied species (Megasporaceae)

This dissertation is focused on the taxonomy, phylogeny, and ecology of the vagrant, erratic and allied terricolous and saxicolous species of the genera Aspicilia A. Massal. and Circinaria Link (Megasporaceae), particularly those traditionally referred to as manna lichens . The group has previously been defined on the basis of few morphological characters. The phylogeny of the family Megasporaceae is inferred from the combined dataset of nuLSU and mtSSU sequences. Five genera Aspicilia, Circinaria, Lobothallia, Megaspora, and Sagedia are recognized. Lobothallia is sister of the four other genera, while Aspicilia and Sagedia form the next clade. All these genera have small asci with eight spores. Circinaria is a sister genus of Megaspora, and these two have in common asci with (1 4) 6 8 large spores. Circinaria forms a monophyletic group and sphaerothallioid species form a monophyletic group within Circinaria. The presence of certain morphological characters such as pseudocyphellae, thickness of cortex and medulla layers, as well as ecological differences in sphaerothallioid species distinguish it from some other crustose species, especially those containing aspicilin and characterised by thin cortex and medulla layers, conidium length c. 6 12 µm and absence of pseudocyphellae. If sphaerothallioid species are accepted as a distinct genus, the rest of the Circinaria species would remain as a paraphyletic assemblage. Currently, the genus Circinaria includes all the sphaerothallioid species and its generic position is confirmed and accepted. Thus, it is proposed as a correct generic name also for the manna lichens described originally in other genera. Phylogeny at the species level was studied using nrITS sequence data. Traditionally, morphological characters have been used for the recognition of species. They were re-evaluated in the light of molecular data. Since characters such as vagrant, erratic and crustose growth forms proved to be misleading for the recognition of some species, a combination of several characters (including molecular data) is recommended. Vagrant growth form seems to have evolved several times among the distantly related lineages and even within a single population. The reasons behind the high plasticity in the external morphology of the sphaerothallioid Circinaria remain, however, unknown. Six new species are recognized: Aspicilia tibetica, Circinaria arida, C. digitata nom provis., C. gyrosa nom. provis., C. rogeri nom. provis., and C. rostamii nom. provis. Based on an analysis of nrITS dataset, three new erratic, vagrant and crustose species were also recognized, but these require additional study. The results also reveal that C. elmorei and C. hispida are not monophyletic as currently understood. In addition, 13 new combinations in the genus Circinaria are proposed.

Housing the skilled migrants in the Helsinki Metropolitan Area : International attractiveness of housing, neighbourhoods and the city region

The aim of this thesis is to examine the skilled migrants’ satisfaction with the Helsinki Metropolitan Area. The examination is executed on three scales: housing, neighbourhoods and the city region. Specific focus is on the built environment and how it meets the needs of the migrants. The empirical data is formed of 25 semi-structured interviews with skilled migrants and additionally 5 expert interviews. Skilled and educated workforce is an increasingly important resource in the new economy, and cities are competing globally for talented workers. With aging population and a need to develop its innovational structure, the Helsinki Metropolitan Area needs migrant workforce. It has been stated that quality of place is a central factor for skilled migrants when choosing where to settle, and from this perspective their satisfaction with the region is significant. In housing, the skilled migrants found the price-quality ratio and the general sizes of apartments inadequate. The housing market is difficult for the migrants to approach, since they often do not speak Finnish and there are prejudices towards foreigners. The general quality of housing was rated well. On the neighbourhood level, the skilled migrants had settled in residential areas which are also preferred by the Finnish skilled workers. While the migrants showed suburban orientation in their settlement patterns, they were not concentrated in the suburban areas which host large shares of traditional immigrant groups. Migrants were usually satisfied with their neighbourhoods; however, part of the suburban dwellers were unsatisfied with the services and social life in their neighbourhoods. Considering the level of the city region, the most challenging feature for the skilled migrants was the social life. The migrants felt that the social environment is homogeneous and difficult to approach. The physical environment was generally rated well, the most appreciated features being public transportation, human scale of the Metropolitan Helsinki, cleanliness, and the urban nature. Urban culture and services were seen good for the city region’s size, but lacking in international comparison.

Männyn (Pinus sylvestris L.) sydänpuun laadun ja määrän jalostamisen mahdollisuudet

Tässä työssä tutkittiin männyn sydänpuun määrän ja laadun jalostamisen mahdollisuuksia sekä sitä, miten sydänpuun ominaisuudet kytkeytyvät toisiinsa. Koska puun kasvu on jalostusohjelmassamme tärkeä jalostustavoite, otettiin kasvutunnuksista tarkasteluun mukaan rinnankorkeusläpimitta ja tutkittiin sen yhteyttä sydänpuun määrään ja laatuun. Erityisesti sydänpuun laatuominaisuuksien on todettu vaikuttavan puuaineen lahonkestävyyteen. Mikäli näihin ominaisuuksiin on mahdollista vaikuttaa jalostamalla, olisi valintaa mahdollista suunnata niin, että kaupalliseen tuotantoon saataisiin puita, joiden luontainen lahonkestävyys olisi nykyistä korkeampi. Tutkimuksen kohteena olleet jälkeläiskokeet sijaitsivat Savonrannalla ja Leppävirralla. Molemmilta jälkeläiskokeilta valittiin tutkittavaksi samat 53 puolisisarperhettä. Savonrannan jälkeläiskokeella otoskoko oli 10 jälkeläistä/perhe. Leppävirralla joistain perheistä tutkittiin vain 9 tai 8 jälkeläistä. Tutkittavista jälkeläisistä otettiin kairanlastunäyte, josta määritettiin sydänpuuosuus, sydänpuun lustojen lukumäärä sekä sydänpuun säde. Lisäksi otettiin näyte kokonaisfenolipitoisuuden ja tiheyden määrittämistä varten. Mitatuista tunnuksista estimoitiin ominaisuuksien heritabiliteetit, additiivisen geneettisen vaihtelun kerroin, ominaisuuksien väliset geneettiset ja fenotyyppiset korrelaatiot sekä kasvupaikkojen väliset geneettiset korrelaatiot. Saadut tulokset osoittavat, että erityisesti sydänpuun laatuominaisuuksien periytyvyys on korkea. Kokonaisfenolipitoisuuden periytyvyysaste oli molemmilla jälkeläiskokeilla 0,54. Tiheyden heritabiliteetti oli Leppävirralla 0,58 ja Savonrannallakin kohtalainen 0,26. Sydänpuun säteen, sydänpuun lustojen lukumäärän sekä sydänpuuprosentin periytyvyysasteet olivat kohtalaisia vain Leppävirran aineistossa. Myös rinnankorkeusläpimitan periytyvyyssaste jäi Savonrannalla selvästi alhaisemmaksi kuin Leppävirralla. Lahonkestävyysjalostuksen kannalta heritabiliteetit vaikuttavat kuitenkin hyviltä. Estimoitujen geneettisten korrelaatioiden perusteella sydänpuun määrän ja laadun samanaikainen jalostus vaikuttaa vaikealta. Geneettisten korrelaatioiden pohjalta pääteltiin myös, ettei kasvua lisäämällä pystytä parantamaan sydänpuun laatua. Sitä vastoin sydänpuun määrän ja kasvun välinen geneettinen yhteys vaikutti selvältä. Laatuominaisuuksien välille estimoitiin kohtalainen fenotyyppinen korrelaatio (0,31 ja 0,34), joten näyttää siltä, että puuaineen tiheyden lisääntyessä myös sen kokonaisfenolipitoisuus lisääntyy. Kasvupaikkojen väliset geneettiset korrelaatiot osoittivat, että samat genotyypit ovat parhaita kaikissa ympäristöissä. Savonrannan jälkeläiskokeella tavattiin alkuvuosina useita bioottisia stressitekijöitä, joilla on voinut olla vaikutusta nyt saatuihin tuloksiin. Lisätutkimus kasvupaikan vaikutuksesta tutkittuihin ominaisuuksiin on tarpeen myös siksi, että tässä tutkimuksessa verratut kasvupaikat olivat hyvin pitkälle toistensa kaltaiset.

Biocompatibility and biofunctionalization of mesoporous silicon particles

Several of the newly developed drug molecules experience poor biopharmaceutical behavior, which hinders their effective delivery at the proper site of action. Among the several strategies employed in order to overcome this obstacle, mesoporous silicon-based materials have emerged as promising drug carriers due to their ability to improve the dissolution behavior of several poorly water-soluble drugs compounds confined within their pores. In addition to improve the dissolution behavior of the drugs, we report that porous silicon (PSi) nanoparticles have a higher degree of biocompatibility than PSi microparticles in several cell lines studied. In addition, the degradation of the nanoparticles showed its potential to fast clearance in the body. After oral delivery, the PSi particles were also found to transit the intestines without being absorbed. These results constituted the first quantitative analysis of the behavior of orally administered PSi nanoparticles compared with other delivery routes in rats. The self-assemble of a hydrophobin class II (HFBII) protein at the surface of hydrophobic PSi particles endowed the particles with greater biocompatibility in different cell lines, was found to reverse their hydrophobicity and also protected a drug loaded within its pores against premature release at low pH while enabling subsequent drug release as the pH increased. These results highlight the potential of HFBII-coating for PSi-based drug carriers in improving their hydrophilicity, biocompatibility and pH responsiveness in drug delivery applications. In conclusion, mesoporous silicon particles have been shown to be a versatile platform for improving the dissolution behavior of poorly water-soluble drugs with high biocompatibility and easy surface modification. The results of this study also provide information regarding the biofunctionalization of the THCPSi particles with a fungal protein, leading to an improvement in their biocompatibility and endowing them with pH responsive and mucoadhesive properties.

Taulukkoaineistojen tilastolliset tietosuojamenetelmät

Tutkielmassa käsitellään taulukkoaineistoihin liittyviä tilastollisia tietosuojakysymyksiä, kuten milloin julkaistavan taulukkoaineiston tilastoyksiköihin kohdistuu paljastumisriski ja millä menetelmillä tätä riskiä voidaan pienentää. Taulukkoaineistojen tilastollisia tietosuojamenetelmiä on tutkittu jo kymmeniä vuosia, mutta edelleen niiden soveltamiseen liittyy haasteita ja ratkaisemattomia kysymyksiä. Taulukkoaineistolla tai taulukolla tarkoitetaan tässä tutkielmassa taulukkomuotoon järjestettyä aggregoitua aineistoa. Yhteiskunta tarvitsee toimiakseen luotettavia tilastotietoja ja tilastoviranomaisten tehtävä on tuottaa niitä. Taulukko on perinteinen muoto julkaista tilastotietoja, joten niille on välttämätön tarve. Paitsi lainsäädäntö, myös tilastoalan eettiset periaatteet edellyttävät tilastoinnin kohteiden yksityisyyden suojaamista. Taulukkoaineisto on näin ollen suojattava ennen julkaisua, jos taulukkoon kohdistuu paljastumisriski. Taulukon paljastumisriski määritellään solukohtaisen paljastumisriskin avulla: taulukkoon kohdistuu riski, jos yhteenkin sen soluun kohdistuu riski. Soluun kohdistuu paljastumisriski, jos yksikin siihen kuuluva tilastoyksikkö on vaarassa paljastua julkaistun taulukon avulla. Paljastumisriskiä ei kokonaan voida poistaa, mutta tilastollisilla tietosuojamenetelmillä se pyritään laskemaan hyväksyttävälle tasolle. Tilastolliset tietosuojamenetelmät muokkaavat aineistoa rajoittamalla, muuntamalla tai korvaamalla, jolloin tietoa väistämättä menetetään. Samalla aineiston laatu, oikeellisuus, tarkkuus, täydellisyys ja käytettävyys kärsivät. Eri menetelmiä sovellettaessa eri määrä informaatiota häviää. Oikean tietosuojamenetelmän valinnassa tärkeimmät kriteerit ovat vaaditun suojaustason saavuttaminen ja taulukon tärkeiden analyyttisten ominaisuuksien säilyminen. Haasteena on löytää kunkin taulukon kohdalla oikea tasapaino tilastoyksiköihin kohdistuvan paljastumisriskin sekä menetetyn tiedon ja hyödyn välillä. Tilastollinen tietosuoja on haastava tilastotieteen osa-alue, koska jokaisella suojattavalla tilastolla on omat huomioonotettavat erityispiirteensä. Voidaan sanoa, että jokainen tilasto muodostaa suojaamisen kannalta oman erikoistapauksensa. Paljastumisriskiä ja erilaisia tilastollisia tietosuojamenetelmiä käsitellään tutkielmassa ensin teoreettisesti. Teoreettinen tarkastelu keskittyy kahteen menetelmään: peittämiseen ja kontrolloituun taulukon säätöön, eli CTA:han (engl. controlled tabular adjustment). Peittäminen on 1970-luvulta asti ollut suosittu suojausmenetelmä, mutta sen myötä taulukosta häviää liikaa tietoa. 2000-luvulla kehitetty vaihtoehtoinen menetelmä CTA pyrkii peittämistä paremmin säilyttämään suojattujen taulukoiden tärkeät ominaisuudet, kuten taulukon additiivisuuden ja soluarvojen jakauman. Tutkielman lopussa on lyhyt empiirinen osa, jossa peittämisen ja CTA:n toimivuutta käytännössä testataan todellisen yritysaineiston avulla. Menetelmiä vertailtaessa tultiin tulokseen, että taulukon laadun ja hyödyn säilyttämisen näkökulmasta CTA on peittämistä parempi menetelmä. Koska CTA on uusi menetelmä, sen soveltamiseen liittyy kuitenkin vielä ongelmia. CTA voi johtaa taulukon käyttäjää harhaan, jos käyttäjä ei ymmärrä menetelmän periaatteita.