Bixin and norbixin protect against DNA-damage and alterations of redox status induced by methylmercury exposure in vivo

Populations in the Amazon are exposed to organic mercury via consumption of contaminated foods. These ethnic groups consume a specific plant seed annatto which contains certain carotenoids. The aim of this study was to find out if these compounds (bixin, BIX and norbixin, NOR), protect against DNA-damage caused by the metal. Therefore, rats were treated orally with methylmercury (MeHg) and with the carotenoids under conditions that are relevant to humans. The animals were treated either with MeHg (30 mu g/kg/bw/day), BIX (0.110 mg/kg/bw/day), NOR (0.011.0 mg/kg/bw/day) or combinations of the metal compound and the carotenoids consecutively for 45 days. Subsequently, the glutathione levels (GSH) and the activity of catalase were determined, and DNA-damage was measured in hepatocytes and leukocytes using single cell gel electrophoresis assays. Treatment with the metal alone caused a decrease in the GSH levels (35%) and induced DNA damage, which resulted in increased DNA migration after electrophoresis in liver and blood cells, whereas no effects were seen with the carotenoids alone. When BIX or NOR were given in combination with organic mercury, the intermediate and the highest concentrations of the carotenoids (1.0 and 10.0 mg/kg/bw/day BIX and 0.1 and 1.0 mg/kg/bw/day NOR) protected against DNA-damage. Furthermore, we found with both carotenoids, a moderate increase in the GSH levels in both metal-treated and untreated animals, while the activities of catalase remained unchanged. Our results indicate that consumption of BIX and NOR may protect humans against the adverse health effects caused by exposure to organic mercury. Environ. Mol. Mutagen., 2012. (c) 2012 Wiley Periodicals, Inc.

HTLV-1 affects the adipogenic differentiation of human mesenchymal stem cells

Financial support: CTC, INCTC, FAPESP, FUNDHERP and CNPq.

Phenolic Constituents, Antioxidant and Preliminary Antimycoplasmic Activities of Leaf Skin and Flowers of Aloe vera (L.) Burm. f. (syn. A. barbadensis Mill.) from the Canary Islands (Spain)

[EN]The methanol extracts of leaf skins and flowers of Aloe vera from the Canary Islands were analyzed for their phenolic profiles and screened for their antioxidant and antimycoplasmic activities. The use of reversed phase high performance liquid chromatography (RP-HPLC) allowed the identification of 18 phenolic constituents. Leaf skin extracts were characterized by the abundance of catechin, sinapic acid and quercitrin. Gentisic acid, epicatechin and quercitrin were the most prominent phenolic compounds of the flowers. The in vitro antioxidant activities determined by using the 1,1-diphenyl-2- picrylhydrazyl (DPPH) and ferric antioxidant reducing power (FRAP) assays revealed that both extracts exhibited antioxidant activity, being the leaf skin extract the most active fraction. The leaf skin extract was also found to be active against the microbial strains tested. Therefore, A. vera extracts from leaf skin and flowers can be considered as good natural antioxidant sources.

Estudio de microorganismos de la clase "Mollicutes" en organismos marinos

Programa de doctorado: Sanidad animal.

Documentos musicales en la prensa de Mendoza : siglo XIX

Con la edición del presente trabajo, entregamos a los estudiosos de la historia regional y socio-cultural un corpus documental referido a la actividad y producción musical desarrolladas en Mendoza en el Siglo XIX, relevado de publicaciones periódicas y legajos institucionales provinciales de esa época que se encuentran en la Hemeroteca Mayor de la Biblioteca Pública General San Martín, en el Archivo General de la Provincia de Mendoza y en la Biblioteca de la Facultad de Filosofía y Letras (Universidad Nacional de Cuyo), conservados en encuadernaciones denominadas "pre-joyas bibliográficas" y en microfilmaciones. Basados en la siguiente hipótesis: "Los documentos históricos de las manifestaciones musicales desarrolladas en Mendoza durante el siglo XIX son testigos fehacientes de esa época, y se determinan como fuentes primarias de información", se trabaja el objetivo general de rescatar nuestro patrimonio musical y, en especial, preservar documentación musical significativa de Mendoza, aportando información fehaciente sobre diferentes aspectos de la historia de la música de nuestra provincia en el siglo XIX. Al tratarse de documentación contenida en soportes perecederos, debe ser rescatada de inmediato, ya que los efectos del tiempo, del uso y de los descuidos involuntarios, hacen que la fuente se deteriore, la información vaya desapareciendo y nuestra historia se vea invadida por grandes huecos de silencios. El relevamiento de datos relacionados con la historia de la música en Mendoza abarca temporalmente desde 1852 a enero de 1902. El momento de inicio de nuestro estudio es 1852, ya que a partir de esa fecha se publica el diario "El constitucional de Los Andes". Para completar cronológicamente el período fijado, se decide continuar con diario "Los Andes".

Documentos musicales en la prensa de Mendoza : siglo XIX : volumen 2

Con la edición del presente trabajo, entregamos a los estudiosos de la historia regional y socio-cultural un corpus documental referido a la actividad y producción musical desarrolladas en Mendoza en el Siglo XIX, relevado de publicaciones periódicas y legajos institucionales provinciales de esa época que se encuentran en la Hemeroteca Mayor de la Biblioteca Pública General San Martín, en el Archivo General de la Provincia de Mendoza y en la Biblioteca de la Facultad de Filosofía y Letras (Universidad Nacional de Cuyo), conservados en encuadernaciones denominadas "pre-joyas bibliográficas" y en microfilmaciones. Basados en la siguiente hipótesis: "Los documentos históricos de las manifestaciones musicales desarrolladas en Mendoza durante el siglo XIX son testigos fehacientes de esa época, y se determinan como fuentes primarias de información", se trabaja el objetivo general de rescatar nuestro patrimonio musical y, en especial, preservar documentación musical significativa de Mendoza, aportando información fehaciente sobre diferentes aspectos de la historia de la música de nuestra provincia en el siglo XIX. Al tratarse de documentación contenida en soportes perecederos, debe ser rescatada de inmediato, ya que los efectos del tiempo, del uso y de los descuidos involuntarios, hacen que la fuente se deteriore, la información vaya desapareciendo y nuestra historia se vea invadida por grandes huecos de silencios. El relevamiento de datos relacionados con la historia de la música en Mendoza abarca temporalmente desde 1852 a enero de 1902. El momento de inicio de nuestro estudio es 1852, ya que a partir de esa fecha se publica el diario "El constitucional de Los Andes". Para completar cronológicamente el período fijado, se decide continuar con diario "Los Andes".

Consumer preferences towards beef cattle in Chile : Importance of country of origin, cut, packaging, brand and price

A study was carried out to evaluate preferences for two cuts, four countries of origin, two forms of presentation, brand and different prices of beef cattle among supermarket buyers in southern Chile, and to distinguish the existence of different market segments, through a survey of 800 people. Using a fractional factorial design for conjoint analysis, it was determined overall that the origin was more important (44.5%) than price (20.8%), form of presentation (12.2%), cut (12.0%) and brand (10.5%), with preference for Chilean and Argentinean striploin, packaged on trays, with no brand at medium price. Using a cluster analysis, three market segments were distinguished. The largest (52.3%) placed great importance on origin and preferred the highest price. The second (27.5%) also valued origin with the greatest preference for Argentinean beef, and it was the only group that preferred the ribeye as the cut. The third (20.5%) placed the greatest importance on price, and was the only group that preferred Paraguayan meat. The segments differed in the importance of eating meat for their personal well-being. The low importance of packaging and brand indicates poorly developed marketing of this product. In order to properly insert brand beef in the Chilean market, communication strategies must be implemented that identify the product with superior quality and that position the brand in the consumer's mind.

Learning kinds for students of technical high school : An analysis from the David Ausubel's theory

This article presents the findings of a field research, not experimental, observational, correlating, basic, of mixed data, micro sociologic, leading to a study of surveys.The object of study is to find learning kinds, and the unit of analysis were 529 high school students between 16 and 21 years old. Its purpose is to understand the impact of learning by rote, guided, self learned and meaningful learning and its achievement degree besides the learning outcomes of differentiated curriculum based on David Ausubel's thoughts, associated with different economic specialties (MINEDUC, 1998) where the population of the study is trained. To collect data, the test TADA - DO2 was used, this test has a reliability index of 0.911 according to Cronbach. From the hits it can be stated from the null hypothesis that there is a significant association (a = 0,05) between the learning kinds and the learning expected of differentiated training plan for both, male and female. It is complex to state that the training of the middle-level technicians leads to a successful employment.

Tipos de aprendizaje en estudiantes de enseñanza media técnico profesional : Un análisis desde la teoría de David Ausubel

Este artículo presenta los hallazgos de una investigación de campo, no experimental, observacional, correlacional, básica, transeccional, de datos mixtos, micro sociológica, dando lugar a un estudio de encuestas. El objeto de estudio son los tipos de aprendizaje y la unidad de análisis fueron 529 estudiantes de enseñanza media Técnico Profesional de NM3 y NM4 entre 16 y 21 años de edad; su propósito es conocer el impacto del aprendizaje memorístico, guiado, autónomo y significativo en el nivel de logro y resultados de aprendizaje del plan de estudio diferenciado, basado en el pensamiento de David Ausubel, asociado a las distintas Areas Económicas y especialidades (MINEDUC, 1998) donde se forman profesionalmente los sujetos de estudio.Para reunir datos se aplicó el test TADA - DO2; la prueba presenta un índice de confiabilidad de 0,911 según Cronbach. Desde los aciertos se puede aseverar desde el enjuiciamiento de la hipótesis nula, que hay asociación significativa a = 0,05) entre los tipos de aprendizaje y los aprendizajes esperados del plan de formación profesional diferenciado tanto para el género masculino como femenino.Indicar que la formación de técnicos de nivel medio para una inserción laboral exitosa de sus titulados, resulta ser una tarea compleja

Learning kinds for students of technical high school : An analysis from the David Ausubel's theory

This article presents the findings of a field research, not experimental, observational, correlating, basic, of mixed data, micro sociologic, leading to a study of surveys.The object of study is to find learning kinds, and the unit of analysis were 529 high school students between 16 and 21 years old. Its purpose is to understand the impact of learning by rote, guided, self learned and meaningful learning and its achievement degree besides the learning outcomes of differentiated curriculum based on David Ausubel's thoughts, associated with different economic specialties (MINEDUC, 1998) where the population of the study is trained. To collect data, the test TADA - DO2 was used, this test has a reliability index of 0.911 according to Cronbach. From the hits it can be stated from the null hypothesis that there is a significant association (a = 0,05) between the learning kinds and the learning expected of differentiated training plan for both, male and female. It is complex to state that the training of the middle-level technicians leads to a successful employment.

Tipos de aprendizaje en estudiantes de enseñanza media técnico profesional : Un análisis desde la teoría de David Ausubel

Este artículo presenta los hallazgos de una investigación de campo, no experimental, observacional, correlacional, básica, transeccional, de datos mixtos, micro sociológica, dando lugar a un estudio de encuestas. El objeto de estudio son los tipos de aprendizaje y la unidad de análisis fueron 529 estudiantes de enseñanza media Técnico Profesional de NM3 y NM4 entre 16 y 21 años de edad; su propósito es conocer el impacto del aprendizaje memorístico, guiado, autónomo y significativo en el nivel de logro y resultados de aprendizaje del plan de estudio diferenciado, basado en el pensamiento de David Ausubel, asociado a las distintas Areas Económicas y especialidades (MINEDUC, 1998) donde se forman profesionalmente los sujetos de estudio.Para reunir datos se aplicó el test TADA - DO2; la prueba presenta un índice de confiabilidad de 0,911 según Cronbach. Desde los aciertos se puede aseverar desde el enjuiciamiento de la hipótesis nula, que hay asociación significativa a = 0,05) entre los tipos de aprendizaje y los aprendizajes esperados del plan de formación profesional diferenciado tanto para el género masculino como femenino.Indicar que la formación de técnicos de nivel medio para una inserción laboral exitosa de sus titulados, resulta ser una tarea compleja

Learning kinds for students of technical high school : An analysis from the David Ausubel's theory

This article presents the findings of a field research, not experimental, observational, correlating, basic, of mixed data, micro sociologic, leading to a study of surveys.The object of study is to find learning kinds, and the unit of analysis were 529 high school students between 16 and 21 years old. Its purpose is to understand the impact of learning by rote, guided, self learned and meaningful learning and its achievement degree besides the learning outcomes of differentiated curriculum based on David Ausubel's thoughts, associated with different economic specialties (MINEDUC, 1998) where the population of the study is trained. To collect data, the test TADA - DO2 was used, this test has a reliability index of 0.911 according to Cronbach. From the hits it can be stated from the null hypothesis that there is a significant association (a = 0,05) between the learning kinds and the learning expected of differentiated training plan for both, male and female. It is complex to state that the training of the middle-level technicians leads to a successful employment.