Association between a 19 bp deletion polymorphism at the dopamine beta-hydroxylase (DBH) locus and migraine with aura

Migraine is a debilitating neurological disorder, affecting 12% of Caucasian populations. It is well known that migraine has a strong genetic component, although the type and number of genes involved is unclear. Our previous work has investigated dopamine related migraine candidate genes and has reported a significant allelic association with migraine of a microsatellite localised to the promoter region of the dopamine beta-hydroxylase (DBH) gene. The present study performed an association analysis in a larger population of case-controls (275 unrelated Caucasian migraineurs versus 275 controls) examining two different genetic DBH polymorphisms (a functional insertion/deletion promoter and a coding SNP A444G polymorphism). Although no significant association was found for the SNP polymorphism, the results showed a significant association between the insertion/deletion variant and disease (chi(2)=8.92, P=0.011), in particular in migraine with aura (chi(2)=11.53, P=0.003) compared to the control group. Furthermore, the analysis of this polymorphism stratified by gender, revealed that male individuals with the homozygote deletion genotype had three times the risk of developing migraine, compared to females. The DBH insertion/deletion polymorphism is in linkage disequilibrium with the previously reported migraine associated DBH microsatellite and this insertion/deletion polymorphism is functional, which may explain a potential role in susceptibility to migraine.

Evidence for allelic association of the dopamine β-hydroxylase gene (DBH) with susceptibility to typical migraine

Migraine is a debilitating neurological disorder characterized by recurrent attacks of severe headache. The disorder is highly prevalent, affecting approximately 12% of Caucasian populations. It is well known that migraine has a strong genetic component, although the type and number of genes involved is not yet clear. However, the calcium channel gene, CACNA1A, on chromosome 19 contains mutations responsible for familial hemiplegic migraine, a rare and severe subtype of migraine. There is also evidence to suggest that serotonin- and dopamine-related genes may be involved in the pathogenesis of migraine. This study employed a linkage and association approach to investigate neurotransmitter-related migraine candidate genes. Polymorphisms within the dopamine beta-hydroxylase (DBH) gene, serotonin transporter gene (SERT), and dopamine receptor gene (DRD2) were tested in 177 unrelated Caucasian migraineurs and 182 control individuals. In addition, an independent sample of 82 families affected with migraine was examined. Unrelated case-control association analysis of a DBH intragenic dinucleotide polymorphism indicated altered allelic distribution between migraine and control groups (L2=16.53, P=0.019). Furthermore, the transmission/disequilibrium test, which was implemented on the family data, also indicated distortion of allele transmission for the same DBH marker (L2=4.44, P=0.035). Together, these results provide evidence for allelic association of the DBH gene with typical migraine susceptibility (Fisher's combined P value=0.006) and indicate that further research into the role of the DBH gene in the etiology of migraine is warranted.

Genero ezberdintasunaren ebaluazioa Asti Leku ikastolako DBH eta Batxilergoko Gorputz Hezkuntzan

Egungo gizartean, nahiz eta azken urteetan aurrerapauso handiak egin diren berdintasunerantz, jarduera fisiko eta kirolaren munduan ezberdintasunak daude oraindik ere generoen artean, nesken kalterako. Nelson Mandelak zioenez, hezkuntza mundua aldatzeko erabili daitekeen armarik indartsuena da. Beraz, zerbait aldatu nahian, kasu honetan, gizartean ematen diren genero ezberdintasun hauek ekiditeko, hezkuntzaz baliatzea aukera paregabea da, arrazoi honengatik, hezkuntzan, heziketa fisikoan bereziki, berdintasuna bilatuko da. Eskola, gizartearen ispilua da, eta ondorioz, bertan ere genero arteko ezberdintasun horiek Heziketa Fisikoarekiko nabarmenak dira. Hezkidetza arazo hau Portugaleteko Asti Leku ikastolan agerikoa da: zentro honetako Heziketa Fisikoko klaseetan genero arteko ezberdintasuna nabaria da arlo honekiko ikasleen jarreran, orokorrean nesken jarrera negatiboagoak eraginda. Zer egin daiteke Asti Leku ikastolan ezberdintasun horiek saihesteko? Galdera honi erantzuna bilatu nahian, genero ezberdintasunean garrantzitsuak izan daitezkeen faktore batzuk aztertuko dira, hala nola, ebaluazioa, eskola, ezberdintasun fisikoak, espazioa, irakasleak, ezkutuko curriculuma eta kirol eskaintza. Ondoren, horiek Asti Leku ikastolan zer nolako eragina duten aztertuko da, horrela zentroko DBH eta Batxilergoan Heziketa Fisikoan ematen den hezkidetza arazoari aurre egiteko zein aldaketa egin daitezkeen ondorioztatzeko, hala nola, kirol-eskaintzan, espazioan eta programazioan.

Joko Sortzailea haur hezkuntzan: DBH-ko Marrazketa Teknikoko ikasleek, prozesu sortzailearen bidez sortutako Haur Hezkuntzarako jokoa

[eus] Lan honen ikerkuntza gaia sormena da. Ibilbide honetan, sormena ardatz eta oinarri hartuz, DBH-ko Marrazketa Teknikoko ikasleek Haur Hezkuntzako umeentzat bideratutako Formak jokoa asmatu dute. Bi erronka nagusi izan ditu lan honek: alde batetik, DBH-ko ikasleekin batera, aholkulari lanak eginez, jokoa eraikitzeko prozesu sortzaile bat aurrera eramatea. Bestetik, HH-ko umeentzat joko sortzaile bat formulatzea. Joko honen helburu nagusiena pertsona guztiengan existitzen den sormen gaitasuna bultzatzea, sustatzea eta garatzea izan delarik. Bidean azaldu diren erronkei aurre egin eta konponbidea topatzerako orduan, egilearen Haur zein Bigarren hezkuntzako hezitzaile perfil bikoitza ezinbesteko tresna izan da. Ikuspuntu bikoitz honek, sormenaren txertatzea hezkuntzako fase bakoitzari egokitzea ahalbidetu du, hezkuntza prozesu globala dela argi izanik.

DBH : Derrigorrezko Bigarren Hezkuntza : beste urrats bat. 'ESO : Educación Secundaria Obligatoria : una nueva etapa'.

Resumen tomado de la web del Departamento de Educación

Ulermenezko irakurketa eta DBH-ko eskola testuak. 'La lectura comprensiva y los textos escolares en la ESO'.

Resumen tomado de la web del Departamento de Educación

Nola prestatu ulermenezko irakurketa plana DBH-ko institutu batean : bi adibide praktiko. 'Cómo elaborar el plan de lectura comprensiva en un instituto de secundaria : dos ejemplos prácticos'.

Primer y segundo premio en la convocatoria de Premios de Fomento de la Lectura del curso 2002-2003 de la modalidad de Educación Secundaria. Incluye el anexo:' El modelo de comprensión lectora. El proyecto Pisa' y cuestionario sobre lectura comprensiva para el profesorado del centro

Lanerako hastapenak eta enplegua bilatzeko teknikak : DBH 2. zikloa. 'Orientaciones laborales y técnicas de búsqueda de empleo'.

Incluye un anexo: Maneja tus contactos

Ingeles arloaren ebaluazioaren txostena. 2. DBH. 2001

Existe una versi??n en espa??ol, con el t??tulo "Informe de la Evaluaci??n de la Lengua Inglesa. 2?? ESO. 2001"

Eskola uztea. DBH-ko bigarren zikloa

Existe una versi??n en espa??ol, con el t??tulo "Abandono escolar. Segundo ciclo de ESO"

Euskararen B2 maila : Derrigorrezko irakaskuntzaren amaieran (DBH-4) : ISEI-IVEI 2005

Existe una versi??n en espa??ol, con el t??tulo "Nivel B2 de euskera : al finalizar la ense??anza obligatoria (4?? ESO) : ISEI-IVEI 2005"

(Table 1) Cover of shrub and tree species, and number of stems (>=3.5 cm DBH) during 1976-1977 and 2009-2010, respectively

Shrubs and trees are expected to expand in the sub-Arctic due to global warming. Our study was conducted in Abisko, sub-arctic Sweden. We recorded the change in coverage of shrub and tree species over a 32- to 34-year period, in three 50 x 50 m plots; in the alpine-tree-line ecotone. The cover of shrubs and trees (<3.5 cm diameter at breast height) were estimated during 2009-2010 and compared with historical documentation from 1976 to 1977. Similarly, all tree stems (>=3.5 cm) were noted and positions determined. There has been a substantial increase of cover of shrubs and trees, particularly dwarf birch (Betula nana), and mountain birch (Betula pubescens ssp. czerepanovii), and an establishment of aspen (Populus tremula). The other species willows (Salix spp.), juniper (Juniperus communis), and rowan (Sorbus aucuparia) revealed inconsistent changes among the plots. Although this study was unable to identify the causes for the change in shrubs and small trees, they are consistent with anticipated changes due to climate change and reduced herbivory.

Left to right: Gustavo Rubio and Melissa Romigh measuring diameter at breast height (DBH) of mangrove trees at SRS-6, Shark River Slough

This material is based upon work supported by the National Science Foundation through the Florida Coastal Everglades Long-Term Ecological Research program under Cooperative Agreements #DBI-0620409 and #DEB-9910514. This image is made available for non-commercial or educational use only.

Association between migraine and a functional polymorphism at the dopamine β-hydroxylase locus

Migraine is a common neurological disorder with a significant genetic component. Although a number of linkage and association studies have been undertaken, the number and identity of all migraine susceptibility genes has yet to be defined. The existence of dopaminergic hypersensitivity in migraine has been recognised on a pharmacological basis and some studies have reported genetic association between migraine and dopamine-related gene variants. Our laboratory has previously reported association of migraine with a promoter STR marker in the dopamine beta hydroxylase (DBH) gene. In the present study, we analysed two additional DBH markers in two independent migraine case–control cohorts. These two markers are putative functional SNPs, one within the promoter (−1021C→T) and another SNP (+1603C→T) in exon 11 of the DBH gene. The results showed a significant association for allelic and genotypic frequency distribution between the DBH marker in the promoter and migraine in the first (P = 0.004 and P = 0.012, respectively) and the second (P = 0.013 and P = 0.031, respectively) tested cohorts. There was no association observed between either genotype and/or allelic frequencies for the DBH marker located in exon 11 and migraine (P ≥ 0.05). The promoter DBH marker, reported associated with migraine in this study, has been shown to affect up to 52% of plasma DBH activity. Varying DBH activity levels have been postulated to be involved in migraine process with an increase of dopamine, resulting from a lower DBH activity shown positively correlated with migraine severity. It is plausible that the functional promoter variant of DBH may play a role in the migraine disorder.