Xenogeneic M2-polarization of Macrophages by undifferentiated and differentiated adipose tissue-derived stem cells

Mesenchymal stem cells (MSCs) are considered to be â â immunologically privileged.â â In a previous work when human adipose tissue-derived stem cells (hASCs) subcutaneously implanted in mice we did not identify an adverse host response1. Recently, it was shown that tissue regeneration could benefit from the polarization of M2 macrophages subpopulations 2. In this study we hypothesised that undifferentiated hASCs and derived osteoblasts and chondrocytes are able to switch murine bone marrow-derived macrophages (mBMMÃ s) into M2 phenotype, aiding tissue regeneration. Murine BMMÃ s were plated in direct contact with undifferentiated and osteo or chondro-differentiated hASCs for 4 h, 10 h, 24 h and 72 h. The cytokine profile was analysed by qRT-PCR and the surface markers were detected by flow cytometry. The direct interaction of both cell types was observed by time lapse microscopy. The results showed that mBMMÃ s polarized after contacting tissue culture polystyrene. This M2 phenotype was maintained along the experiment in direct contact with both undifferentiated and osteo or chondro-differentiated hASCs. This was confirmed by the expression of IL-1, IL-10, IL-4, TNF-a and IFN-g (genetic profile) and surface markers (CD206 + + , CD336 + + , MHC II + and CD86 + + ) detection. These data suggest the potential of hASCs in contemporary xenogenic tissue engineering and regenerative medicine strategies, as well as host immune system modulation in autoimmune diseases. 

The role of IFNγ in higher brain function: in health and under chronic stress

Tese de Doutoramento em Ciências da Saúde

The impact of poor power quality in solar photovoltaic microgeneration: technical problems and losses of revenue

This paper presents a critical and quantitative analysis of the influence of the Power Quality in grid connected solar photovoltaic microgeneration installations. First are introduced the main regulations and legislation related with the solar photovoltaic microgeneration, in Portugal and Europe. Next are presented Power Quality monitoring results obtained from two residential solar photovoltaic installations located in the north of Portugal, and is explained how the Power Quality events affect the operation of these installations. Afterwards, it is described a methodology to estimate the energy production losses and the impact in the revenue caused by the abnormal operation of the electrical installation. This is done by comparing the amount of energy that was injected into the power grid with the theoretical value of energy that could be injected in normal conditions. The performed analysis shows that Power Quality severally affects the solar photovoltaic installations operation. The losses of revenue in the two monitored installations M1 and M2 are estimated in about 27% and 22%, respectively.

Estudo experimental e numérico sobre o comportamento de vigas mistas com conector Crestbond

Nesta comunicação apresenta-se um estudo sobre vigas mistas em aço e betão onde se utilizam conectores do tipo Crestbond contínuos para estabelecer a ligação entre a viga metálica e a laje de betão e garantir o funcionamento misto entre estes dois elementos. Apresentam-se os resultados de ensaios numéricos e experimentais obtidos em vigas mistas de aço e betão realizadas com conectores Crestbond, na Universidade do Minho. Os provetes consistem numa viga metálica IPE200 com um conector CR40 R10 contínuo, posicionado sobre o banzo superior da viga e soldado em todo o seu desenvolvimento, e uma laje betão armado com secção transversal de 0.40×0.10 m2. Os provetes apresentam um vão de 3.0 m e um comprimento total de 3.2 m. O modelo numérico foi desenvolvido no software Atena 3D.

Ensaio à rotura de protótipo de edifício em lajes de betão auto-compactável reforçado com fibras

O presente artigo descreve a investigação experimental realizada para avaliar as potencialidades da utilização de betão auto-compactável reforçado com fibras de aço (BACRFA) na construção de lajes fungiformes de edifícios de habitação e comércio, onde as fibras são o único reforço aplicado nas lajes. Para tal foi construído um protótipo deste tipo de edifícios, à escala 1:4, e ensaiado para condições de carga que permitissem avaliar o seu comportamento em serviço e em rotura. Estes ensaios foram complementados por um extenso programa experimental de caracterização do comportamento mecânico do BACRFA. Os ensaios de fluência no protótipo demonstraram a não ocorrência de fendas, bem como uma deformação dentro dos limites aceitáveis para este tipo de construção até uma sobrecarga de aproximadamente 40 kN/m2. No ensaio à rotura, o protótipo desenvolveu elevada capacidade de carga e rotura dúctil por flexão com formação de padrão difuso de fendas. Os resultados obtidos evidenciaram que o BACRFA desenvolvido demonstra potencial para ser utilizado no tipo de aplicação em causa com benefícios técnico e económicos.

Modeling microbes: New methods for integrated metabolic and regulatory network reconstruction

PhD Thesis in Bioengineering

Improvement of in silico strain engineering methods in Saccharomyces cerevisiae

PhD thesis in Bioengineering

Cationic Liposomes as antigenic delivery systems: development of an immunoprotective strategy against Candida albicans

Tese de Doutoramento Biologia Molecular e Ambiental - Especialidade em Biologia Celular e Saúde

Aging and Alzheimer's disease: Searching for novel molecular cues

Tese de Doutoramento em Ciências da Saúde

A cost study of common surface finish systems for steel structures

This paper presents part of a study aimed at finding a suitable, yet cost-effective, surface finish for a steel structure subject to the car washing environment and corrosive chemicals. The initial, life cycle and average equivalent annual (AEAC) costs for surface finishing methods were calculated for a steel structure using the LCCC algorithm developed by American Galvanizers Association (AGA). The cost study consisted of 45 common surface finish systems including: hot-dip galvanization (HDG), metallization, acrylic, alkyd and epoxy as well as duplex coatings such as epoxy zinc and inorganic zinc (IOZ). The results show that initial, life cycle and AEAC costs for hot dip galvanization are the lowest among all the other methods, followed by coal tar epoxy painting. The annual average cost of HDG for this structure was estimated about €0.22/m2, while the other cost-effective alternatives were: IOZ, polyurea, epoxy waterborne and IOZ/epoxy duplex coating.

Aplicação de técnicas e procedimentos Lean Production numa empresa da indústria automóvel

Dissertação de mestrado integrado em Engenharia e Gestão Industrial

Pseudomonas aeruginosa diversification during infection development in cystic fibrosis lungs

Dissertação de mestrado integrado em Engenharia Biomédica (área de especialização em Engenharia Clínica)

Mesenchymal stem cells culture on poly(vinylidene fluoride) piezoelectric microspheres

Dissertação de mestrado em Biofísica e Bionanossistemas

The impact of carbon source in Candida albicans virulence: participation of RLM1 in pathogen host interaction

Dissertação de mestrado em Genética Molecular

Exploring the role of O-Mannosylation in the modulation of E-Cadherin function in Gastric Cancer cell line models

Dissertação de mestrado em Bioquímica Aplicada – Biomedicina