Preliminary Investigation of Fluid Flow in Meso-Scale Rectangular Tube

The fluid flow associated with micro and meso scale devices is currently of interest. Experiments were performed to study the fluid flow in meso-scale channels. A straight flow tube was fabricated with 1.0x4.0mm^2 in rectangular cross section and 200mm in length, which was made of quartz for flow visualization and PIV measurements. Reynolds numbers were ranged from 311 to over 3105. The corresponding pressure drop was from 0.65KPa to over 16.58KPa between the inlet and outlet of the tube. The micro PIV was developed to measure the velocity distribution in the tube. A set of microscope object lens was mounted ahead of CCD camera to obtain optimized optical magnification on the CCD chip. The velocity distributions near the outlet of the tube were measured to obtain full-developed flow. A CW laser beam was focused directly on the test section by a cylinder lens to form a small light sheet. Thus, high power density of light was formed on the view region. It is very important to the experiment while the velocity of the flow reaches to a few meters per second within millimeter scale. In this case, it is necessary to reduce exposure time to microseconds for PIV measurements. In the present paper, the experimental results are compared with the classical theories.

Generation of double pulses in-line by using reflective Dammann gratings

Doubled femtosecond laser pulses in-line are needed in the collinear pump-probe technique, collinear second harmonic generation frequency-resolved optical gating (SHG FROG) and the spectral phase interferometry for direct electric-field reconstruction (SPIDER), etc. Normally, it is generated by using a Michelson's structure. In this paper, we proposed a novel structure with two-layered reflective Dammann gratings and the reflective mirrors to generate doubled femtosecond laser pulses in line without transmission optical elements. Angular dispersion and spectral spatial walk-off are both compensated. In addition, this structure can also compress the positive chirped pulse, which cannot be realized with a Michelson's structure. By adopting triangular grating and blazed gratings, the efficiency of the system would in principle be increased as the Michelson's scheme. Experiments demonstrated that this method should be an alternative approach for generation of the double compressed pulses of femtosecond laser for practical applications. (c) 2006 Elsevier GmbH. All rights reserved.

Homoepitaxial growth of ZnO films via low-temperature liquid-phase epitaxy

Homoepitaxial ZnO films have been grown via liquid-phase epitaxy (LPE) on (000 1) oriented ZnO substrates. X-ray rocking curve revealed the high quality of the ZnO films with a FWHM of 40 arc sec. Films of thickness about 20 gm were gown in the temperature range 700-720 degrees C. The growth rate of ZnO films was estimated to be 0.3 mu m h(-1). Atomic force microscope analysis showed that the surface roughness of ZnO films was very low, which further confirmed the high crystallinity of ZnO films. (c) 2006 Elsevier B.V. All rights reserved.

ISOLATION AND PROPERTIES OF A BLOOD-COAGULATION FACTOR-X ACTIVATOR FROM THE VENOM OF KING COBRA (OPHIOPHAGUS HANNAH)

A specific blood coagulation factor X activator was purified from the venom of Ophiophagus hannah by gel filtration and two steps of FPLC Mono-Q column ion-exchange chromatography. It showed a single protein band both in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and alkaline polyacrylamide gel electrophoresis. The mol. wt was estimated to be 62,000 in non-reducing conditions and 64,500 in reducing conditions by SDS-PAGE. The isoelectric point was found to be pH 5.6. The enzyme had weak amidolytic activities toward CBS 65-25, but it showed no activities on S-2266, S-2302, thrombin substrate S-2238, plasmin substrate S-2251 or factor Xa substrate S-2222. It had no arginine esterase activity toward substrate benzoylarginine ethylester (BAEE). The enzyme activated factor X in vitro and the effect was absolutely Ca2+ dependent, with a Hill coefficient of 6.83. It could not activate prothrombin nor had any effect on fibrinogen and thus appeared to act specifically on factor X. The procoagulant activity of the enzyme was almost completely inhibited by serine protease inhibitors like PMSF, TPCK and soybean trypsin inhibitor; partially inhibited by L-cysteine. Metal chelator EDTA did not inhibit its procoagulant activity. These results suggest that the factor X activator from O. hannah venom is a serine protease.

AN ACTIVATOR OF BLOOD-COAGULATION FACTOR-X FROM THE VENOM OF BUNGARUS-FASCIATUS

A specific activator of blood coagulation factor X was purified from the venom of Bungarus fasciatus by gel filtration and by ion-exchange chromatography on a Mono-Q column (FPLC). It consisted of a single polypeptide chain, with a mel. wt of 70,000 in reducing and non-reducing conditions. The enzyme had an amidolytic activity towards the chromogenic substrates S-2266 and S-2302 but it did not hydrolyse S-2238, S2251 or S-2222, which are specific substrates for thrombin, plasmin and factor Xa, respectively. The enzyme activated factor X in vitro and the effect was Ca2+ dependent with a Hill coefficient of 7.9. As with physiological activators, the venom activator cleaves the heavy chain of factor X, producing the activated factor Xa alpha. The purified factor X activator from B. fasciatus venom did not activate prothrombin, nor did it cleave or clot purified fibrinogen. The amidolytic activity and the factor X activation activity of the factor X activator from B. fasciatus venom were readily inhibited by serine protease inhibitors such as diisopropyl fluorophosphate (DFP), phenylmethanesulfonyl fluoride (PMSF), benzamidine and by soybean trypsin inhibitor but not by EDTA. These observations suggest that the factor X activator from B. fasciatus venom is a serine protease. It therefore differs from those of activators obtained from Vipera russelli and Bothrops atrox venoms, which are metalloproteinases.

菜花烙铁头蛇毒中一种具有激肤释放酶与纤维蛋白原溶酶活性的Jerdonase

通过DEAF Sephadex t-50阴离子交换、超细Sephadex 6100分子筛和反相高效液相CQ色谱层析，从菜花烙铁头蛇毒冻干粉中纯化出一种具有激肤释放酶活性和。纤维蛋白原溶酶活性的丝氨酸蛋白酶，命名为Jerdonase。在12 .5%胶浓度的SD6还原电泳条件下，该酶分子量大约为55 kD，在非还原电泳条件下，分子量大约为53 kD。此酶是一种糖蛋白，含有约35 .8%的中性糖。它的N末端氨基酸序列为IIGGDEENINEHPFLVALYDA，其序列和蛇毒中其他丝氨酸蛋白酶具有非常高的序列相似性。Jerdonase能够催化BAEE、 S-2238和S-2302的水解，其水解活性可被PMSF抑制，但是EDTA对此没有影响。Jerdonase能优先水解人纤维蛋白原的An链，同时伴随有微弱的B[3链水解活性。另外，此酶能够水解牛低分子量的激肤原，释放舒缓激肤。总之，所有的结果表明Jerdonase是一个具有多功能活性的蛇毒丝氨酸蛋白酶。

Jerdonase, a novel serine protease with kinin-releasing and fibrinogenolytic activity from Trimeresurus jerdonii venom

A novel kinin-releasing and fibrin (ogen)olytic enzyme termed jerdonase was purified to homogeneity from the venom of Trimeresurus jerdonii by DEAE Sephadex A-50 anion exchange, Sephadex G-100 (superfine) gel filtration and reverse-phase high performance liquid chromatography (RP-HPLC). Jerdonase migrated as a single band with an approximate molecular weight of 55 kD under the reduced conditions and 53 kD under the non-reduced conditions. The enzyme was a glycoprotein containing 35.8% neutral carbohydrate. The N-terminal amino acid sequence of jerdonase was determined to be IIGGDECNINEHPFLVALYDA, which showed high sequence identity to other snake venom serine proteases. Jerdonase catalyzed the hydrolysis of BAEE, S-2238 and S-2302, which was inhibited by phenymethylsulfonyl fluoride (PMSF), but not affected by ethylenediaminetetraacetic acid (EDTA). Jerdonase preferentially cleaved the Aalpha-chain of human fibrinogen with lower activity towards Bbeta-chain. Moreover, the enzyme hydrolyzed bovine low-molecular-mass kininogen and releasing bradykinin. In conclusion, all results indicated that jerdonase was a multifunctional venom serine protease.

扫描电镜细胞样品制备方法的改进

国家自然科学基金资助项目 (30 170 72 6 ) ; 86 3计划项目 (2 0 0 1AA6 2 6 0 30和 2 0 0 2AA6 2 6 0 10 ) ) ; 中国科学院及其水生生物研究所院创新项 目 (KSCX2 SW 30 2和 2 2 0 313)

东亚低等鲤科鱼类细胞色素b基因序列测定及系统发育

采用PCR技术获得了14种主要分布于东亚的低等鲤科鱼类细胞色素b基因的全序列. 所得1 140 bp细胞色素b基因序列与10种取自GenBank, 分布在北美和欧洲的相关鲤科鱼类的同一基因序列一起排序后, 得到了24种鲤科鱼类的DNA序列矩阵. 此矩阵经过最大似然(maximum likelihood)法计算后获得了低等鲤科及相关种类的系统发育分支图解. 分支系统图显示鲤科的雅罗鱼亚科和亚科鱼类并不形成单系类群. 亚科鱼类中的马口鱼、等是原始的鲤科鱼类, 处于分支图的基部. 而其余的亚科鱼类则分散

AMPS modeling of light J-V characteristics of a-Si based solar cells

AMPS (Analysis of microelectronic and photonic structures) mode,which was developed by Pennsylvania State University, has been used to module the light J-V characteristics of a-Si solar cells with a structure of TCO/p-a-SiC:H/i-a-Si:H/n-a-Si:H/ metal. The effects of valence band offset and contact barriers at p/i and TOC/p, n/metal interfaces on the light J-V characteristics have been examined. The modeling has qualitatively categorized and explained the non-ideal J-V behaviors (rollover, crossover, Voc shift,and rollunder) observed in a-Si based solar cells.

可满足性问题研究

数理逻辑是计算机科学的基础，而逻辑公式的可满足性问题是计算机科学和人工智能领域中的一个著名而又重要的问题。它在计算机科学和人工智能研究中有广泛的应用。本文将重点介绍实际可用的判定算法及其相关技术。 本文的研究分两大方向，分别是命题逻辑的可满足性问题研究和一阶逻辑的可满足性问题研究。命题逻辑的可满足性问题（Satisfiability Problem）是一个历史悠久的问题，一般简称为 SAT问题。SAT 问题的求解算法有两大类，分别是确定性算法和非确定性算法。本文只研究确定性算法的相关内容，尤其是如何进行剪枝。我们充分调研了SAT求解算法和求解工具的相关知识，在此基础上，提出了local Lemma 这个剪枝技术，该技术可以更加充分地挖掘剪枝信息，达到进行更多剪枝的目的。 在一阶逻辑的可满足性问题研究方面，本文采取直接搜索可行的一阶逻辑模型的方法研究有限论域上的一阶逻辑的可满足性问题。该方法本质上就是深度优先的回溯搜索。因此，我们的研究重点就是如何找到问题的更高效的一阶逻辑的表示以及如何采用启发式的方式进行剪枝。在这个问题上，我的主要工作有两个：一个是模型搜索中适用于谓词的同构消去技术，包括Row by Row策略和Ramsey Number 策略两个技术；另一个是 DASH（Decision Assignment Scheme Heuristic），用来在生成有限模型的时候边搜索边消除同构子空间。DASH 能用近乎零代价保证输出的模型都是互不同构的。这两个技术都是基于有限论域中的元素的固有对称性，换言之，就是需要有限论域中的元素是两两可交换的。 在已有的成果的基础上，我们又尝试使用它们去求解一些数学中的实际问题，如拟群拉丁方，有限射影平面，正交拉丁方（组）等。我们给出了在这几个方面的一些尝试和初步结果，包括一个重要的定理和若干效率提高了几个数量级的改进。 值得一提的是，和马菲菲共同提出的横截矩阵（Transversal Matrix）概念是一个新颖的成果，使得正交拉丁方的表示和求解取得了明显的改进。在这个概念的基础上，进一步给出了几个定理，进行了理论上的拓展。实验数据表明，我们的求解途径和技术能有效解决拉丁方相关的问题。

基于HMM-FNN模型的复杂动态手势识别

复杂动态手势识别是利用视频手势进行人机交互的关键问题.提出一种HMM-FNN模型结构.它整合了隐马尔可夫模型对时序数据的建模能力与模糊神经网络的模糊规则构建与推理能力,并将其应用到复杂动态手势的识别中.复杂动态手势具备两大特点:运动特征的可分解性与定义描述的模糊性.针对这两种特性,复杂手势被分解为手形变化、2D平面运动与Z轴方向运动3个子部分,分别利用HMM进行建模,HMM模型对观察子序列的似然概率被作为FNN的模糊隶属度,通过模糊规则推理,最终得到手势的分类类别.HMM-FNN方法将高维手势特征分解为低维子特征序列,降低了模型的复杂度.此外,它还可以充分利用人的经验辅助模型结构的创建与优化.实验表明,该方法是一种有效的复杂动态手势识别方法,并且优于传统的HMM模型方法.

非晶硅太阳电池光照J-V特性的AMPS模拟

运用AMPS(Analysis of Microelectronic and Photonic Structures)模拟分析了TCO/p-a-SiC:H/i-a-Si:H/n-a-Si:H/metal结构的异质结非晶硅太阳电池中的p/i界面的价带失配以及TCO/p,n/metal界面接触势垒对电池光电特性的影响.分析总结了非晶硅基薄膜太阳电池中J-V曲线异常拐弯现象的种类和可能原因.

论中国海洋经济的可持续发展

发展海洋经济是解决当前人类所面临的资源匮乏、空间紧张、环境恶化等问题的一条有效途径 ,开发海洋这一尚未被充分利用的资源宝库也已上升为许多国家和地区的战略性问题。我国主要资源的人均占有量均低于世界平均水平 ,而快速发展的经济对资源的需求又不断增加 ,使得海洋资源的开发利用在我国经济发展中的地位越来越重要。丰富的生物、港口、矿产、旅游等资源 ,有利的地理位置 ,显示了我国发展海洋经济的优越性。近年来 ,我国海洋经济发展较快 ,特别是海洋运输、旅游、海上油气开采等新兴产业发展迅速 ,但同时也存在第一产业比重过高、资源开发不合理、海洋生态环境恶化、海洋灾害频繁、领土争议等困扰可持续发展的一系列问题。为了实现我国海洋经济的可持续发展 ,应该做到 :①实施以淘汰高污染产业、发展清洁及环保技术的可持续发展的战略 ;②加强包括宣传、规划、环境治理、防灾减灾在内的综合管理 ;③发展深海开发、海洋生物和海洋探测等技术 ,走“科技兴海”之路 ;④大力发展海洋油气、旅游、生物、化工等新兴产业 ,优化产业结构