The Biology of a High Isolated Tide Pool

A small isolated tide pool was studied quite intensively over a period on one month. A oensus of all animals present was taken, and a population record kept daily for the month. Fluctuations in the numbers of individuals were noted, and reasons for these fluctuations sought. The behavior and feeding habits of the various animals were noted, and an attempt was made to relate the animals to their environment. This is a student paper done for a University of California Berkeley Zoology class. Since UCB didn't have its own marine lab at the time, it rented space at Hopkins Marine Station where this work was done. Gene Haderlie went on to earn his Ph.D. from Berkeley and later became a Professor at the Naval Post Graduate School in Monterey. (PDF contains 22 pages)

Acyclic diterpene alcohols isolated from four algae of Bryopsidophyceae and their toxicity

Three new acylic diterpenoids belonging to the class of phytol series have been isolated. They were obtained from the ethyl acetate soluble fractions of four siphonaceous green seaweeds, Bryopsis pennata Lamour., Caulerpa taxifolia (Vahl) C. Ag., Codium decorticatum (Woodw.) Howe and Valoniopsis pachynema (Mart.) Børg., collected from Karachi coast of Pakistan. Structures of these compounds were elucidated with the help of spectroscopic methods and confirmed by comparison with the known compounds. Even the known compounds are being reported for the first time from a green algal source. All the compounds were found to display a strong toxicity at all the three concentrations tested in the brine shrimp bioassay.

Growth and reproductive performance of locally isolated brackish water rotifer (Brachionus plicatilis) feeding different micro algae

Population growth and reproductive capacity of brackishwater rotifer, Brachionus plicatilis, were evaluated, for a period of 8 days in a temperature controlled ( =25°C) microalgallaborarory, under three different algal feeding regimens. The algal species that were tested are: (i) Chlorella sp. (T1), Tetraselmis chui (T2), Nannochloropsis oculata (T 3). The feeding density of each algal species was maintained similar as of 4.5xW6 ceHs mi. The rotifer fed on T. chui showed the highest (p<0.05) population growth (131.5 ind./ml), compared to that fed on Chlorella sp (45.67 ind./ml) and N oculata (43.44 ind./ml). The abundance of egg bearing rotifers was also higher (35.77%) with T. chuithan with Chlorella sp (27.76%) and N oculara (24.60%). The results of the present study indicate that T. chui could be the most suitable algal food for the stock culture of locally isolated rotifer B. plicatilis.

Studies on the growth temperature ranges of bacteria isolated from fresh sardine at different primary incubation temperature

The effect of primary incubation temperature on the growth temperature range was studied with reference to 296 bacterial cultures isolated from sardine using streak plate technique. The primary incubation temperature used during bacteriological sampling caused a selection of strains according to their growth temperature requirements. Incubation at 8°C caused greater recoveries of psychrotrophs while 30°C favored mesophiles. An incubation temperature of 30°C facilitated the growth of both psychrotrophs and mesophiles.

Studies on the chemical control of psychrophilic bacterial spoilage of fish. 2 - The effect of antibiotics on the growth of psycrophilic bacteria isolated from marine fish

Among the various antibiotics tried, tetracyclines particularly chlorotetracycline (CTC), chloramphenicol and chlorostrep were found to be fairly effective at 8 and 10 p.p.m. levels. The order of sensitivity to CTC among the six genera studied was found to be Achromobacter

Studies on the chemical control of psychrophilic bacterial spoilage of fish. 4 - The effect of chemical preservatives on the growth of psychrophilic bacteria isolated from marine fish

Dehydroacetic acid and ammonia were found to be very effective in checking the growth of all the cultures at all concentrations tried. The two nitrofuran derivatives namely, semicarbazone and AF-2 were fairly effective, semicarbazone being more effective than AF-2. Sodium nitrite was found to be totally ineffective against the cultures at all concentrations tried.

Some bacteria isolated from marine plankton and mud

57 bacterial strains isolated from marine plankton and mud from the inshore waters of Cochin, are described. The flora of the plankton was dominated by chromogens while mud sample and failed to produce chromogens. The different strains are divided into five arbitrary groups for the convenience of description. The flora is similar to that of Mandapam (east coast) while it differed from the flora of Calicut (west coast) in the paucity of Bacillus and other spore formers.

Morphological, biochemical and growth characteristics of Serratia strains isolated from sardine (Sardinella longiceps)

Two aerobic, gram-negative asporogenous, red-pigmented, rod-shaped bacterial strains were isolated from oil sardine (Sardinella longiceps). Their morphological, biochemical and growth characteristics are reported. The pigment was identified to be a prodigiosene. The strains were found to resemble Serratia plymuthica. Effect of temperature and certain carbohydrates on pigmentation was also studied. Iron was found to inhibit pigmentation, and mannitol or sorbitol removed such inhibition.

Soluble protein isolated from low cost fish and fish wastes

The method of preparation, composition, amino acid content, protein efficiency ratio and areas of possible application of water soluble protein isolates from low cost fish and fish wastes are discussed in detail in this communication.

Salt tolerance of bacteria isolated from tropical marine fish and prawn

Salt tolerance of selected cultures of Pseudomonas, Moraxella, Vibrio, Micrococcus, Acinetobacter and Flavobacteria/ Cytophaga was determined. More than 80% of the cultures belonging to each of the above genera, were capable of growth in presence of 1.5 to 3.5% salt (NaCl) and at least 25 to 30% of the cultures in each group required 1.5 to 3.5% salt for growth. 40% each of Pseudomonas and Vibrio strains and 30% each of Moraxella, Micrococcus and Flavobacteria/Cytophaga strains tolerated 10% salt. Majority of the cultures belonging to the genera Pseudomonas, Vibrio, Moraxella, Micrococcus, Acinetobacter and Flavobacteria/Cytophaga were slightly halophilic (2 to 5% salt tolerant), about 25% especially of Micrococcus spp. moderately halophilic (5 to 20% salt tolerant) and none from Pseudomonas, Vibrio, Moraxella, Acinetobacter and Flavobacteria/Cytophaga spp. extremely halophilic (20 to 32% salt tolerant).

Antibiotic sensitivity of Staphylococcus aureus strains isolated from fishery products

During the course of an investigation on the incidence of Staphylococcus aureus contamination in frozen crab meat, frozen prawns and dried fishes from Cochin, 116 strains of S. aureus were isolated. The sensitivity of the isolated strains towards nine antibiotics showed that all the S. aureus strains were sensitive to kanamycin and streptomycin (100%). Sensitivity to other antibiotics like chloramphenicol, polymyxin-B, erythromycin, tetracycline, neomycin, penicillin and ampicillin were shown by 98.28, 93.10, 87.93, 68.10, 67.24, 56.90 and 55.17 percent of the isolates respectively.

Studies on Thiobacilli spp. isolated from sandy beaches of Kerala

Occurrence, isolation and oxidative activity of Thiobacilli spp. from some sandy beaches of Kerala are reported. These organisms were encountered in polluted beaches and were dominant during monsoon in all the beaches.

Antibiotic resistance of Staphylococcus aureus strains isolated from fish processing factory workers

One hundred and twenty two strains of Staphylococcus aureus isolated from throats and palms of 39 workers from 6 fish processing factories situated in and around Cochin were tested for their sensitivity to nine commonly used antibiotics-ampicillin, chloramphenicol, erythromycin, kanamycin, neomycin, penicillin, polymyxin-B, streptomycin and tetracycline. Highest percentage of resistance was observed towards ampicillin followed by penicillin i.e. 64.75% and 59.84%. Resistance towards other antibiotics like tetracycline, polymyxin-B, erythromycin, kanamycin, neomycin, chloramphenicol and streptomycin were shown by 22.95, 16.39, 7.38, 5.74, 3.28 and 1.64% of the isolates respectively.