8 resultados para Amino acid specificity

em Aquatic Commons


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Numerous investigations have utilized various semi-purified and purified diets to estimate the protein and amino acid requirements of several temperate fishes. The vast literature on the protein and amino acid requirements of fishes has continued to omit that of the tropical warm water species. The net effect is that fish feed formulation in Nigeria have relied on the requirement for temperate species. This paper attempts to review the state of knowledge on the protein amino acid requirements of fishes with emphasis on the warm water species, the methods of protein and amino acid requirement determinations and the influence of various factors on nutritional requirement studies. Finally evidence are presented with specific examples on how requirements of warm water fishes are different from the temperate species and used this to justify why fish feed formulation in Nigeria are far from being efficient

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Four species of Gracilaria are investigated for their free amino-acid contents, as well as amino-acid constituents in the proteins and the peptides, using quantitative paper chromatographic technique. Amino-acid constituents of different species of Gracilaria differ only in amount, while free amino-acids and the amino-acids in the peptides vary both in quality and quantity. A number of amino-acids recorded as protein constituents have even escaped detection in the peptides, while in the free state they occur either in all the species or in some only except homocystine. Moreover, some amino-acids occur exclusively in the free state.

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Caulerpa racemosa var. macrophysa, C. racemosa var cornyphora and C. scalpelliformis are analyzed by quantitative paper chromatographic technique for their amino acid contents in proteins, peptides and free state. It is found that no appreciable variation occurs in the quality of amino acid make up in these algae; but quantitative differences are apparent in them. Moreover, both qualitative and quantitative variations occur in the amino acid make up of the peptides, and in the free state. The results are compared with those of the other investigations.

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This study document effects of short-term (96h) sublethal levels of copper, cadmium and their mixture on the amino acid composition of postlarvae of the penaeid shrimp, P.monodon and P.penicillatus . All experimental conditions were kept constant, temperature between 25-27•C and salinity 21-22 ppt. The estimated LD50 for Cu was 200 ug/L, for Cd 177.5 ug/L and for Cu.Cd mixture 250ug/L. In P. penicillatus at the same concentration of each metal, there was significant reduction in amino acid content, which was 8.01% higher than the control. Almost similar reduction in some amino acids was observed in P.monodon. At the maximum concentration of 400 ug/L, cadmium caused higher reduction in amino acid composition than did copper. Thus, amino acid composition may be regarded as a sensitive biochemical indicator of Cu and Cd toxicity because of the effect of these metals on protein synthesis, a signal of physiological stress in marine organisms subjected to heavy metal pollution.

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The moisture and free alpha amino nitrogen contents of some important food fishes and shell fishes of Kakinada region have been studied. Crustaceans and molluscs contain free alpha amino acids in quantities several times higher than all other aquatic animals examined in this study. Their probable role in the physiological activities of these animals has been discussed.

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Details are given of the yield and composition of dried waste from the filleting wastes of 3 commercially less utilized fish of the Maharashtra coast (Saurida tumbil, Caranx sexfasciatus and Sphyraena jello). The amino acid composition after acid hydrolysis is detailed for the three species.

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The amino acid composition was determined of three economic important fish species of Lake Tanganyika, representing 95% of the tolal catch:Stolothrissa tanganicae Regan, Limnothrissa miodon Boulenger and Luciolates stapersii Boulenger. The analysis of the whole sun dried fish indicate that, compared with Tilapia macrochir, beef and the whole hen's egg, the fish is of a very high nutritive value. The free amino acids in the dried fish count for an average of 11 %, indicating the degree of auteolysis.