An orthotopic skull base model of malignant meningioma

Meningioma tumor growth involves the subarachnoid space that contains the cerebrospinal fluid. Modeling tumor growth in this microenvironment has been associated with widespread leptomeningeal dissemination, which is uncharacteristic of human meningiomas. Consequently, survival times and tumor properties are varied, limiting their utility in testing experimental therapies. We report the development and characterization of a reproducible orthotopic skull-base meningioma model in athymic mice using the IOMM-Lee cell line. Localized tumor growth was obtained by using optimal cell densities and matrigel as the implantation medium. Survival times were within a narrow range of 17-21 days. The xenografts grew locally compressing surrounding brain tissue. These tumors had histopathologic characteristics of anaplastic meningiomas including high cellularity, nuclear pleomorphism, cellular pattern loss, necrosis and conspicuous mitosis. Similar to human meningiomas, considerable invasion of the dura and skull and some invasion of adjacent brain along perivascular tracts were observed. The pattern of hypoxia was also similar to human malignant meningiomas. We use bioluminescent imaging to non-invasively monitor the growth of the xenografts and determine the survival benefit from temozolomide treatment. Thus, we describe a malignant meningioma model system that will be useful for investigating the biology of meningiomas and for preclinical assessment of therapeutic agents.

Book Review: "Rethinking Learning in Early Childhood Education" written by Nicola Yelland, Libby Lee, Maureen O’Rourke & Cathie Harrison

There is a growing body of work that responds to the impact of the rapid uptake of information and communication technology (ICT) on education (Buckingham, 2003; Cheung, 2003; Cuban, 2003; Leung, 2003; Prensky, 2005; Green & Hannon, 2007; Brooks-Gunn & Donahue, 2008; Lyman et al, 2008). Mostly, this work has been positioned in the context of upper-primary or secondary classrooms. More recently, there has been a growing call for research about the impact of ICT on the early years or in early childhood contexts. This text initiates a response to that call. The authors concur that today’s children are a generation who create, learn, work, play and communicate very differently from their parents and teachers (Buckingham, 2003), and that classroom activity needs to reflect this difference.

Susan Carson in conversation with Donna Lee Brien on research higher degree examination administration

This paper is the edited transcript of a conversation between Susan Carson and Donna Lee Brien about an administrator’s perspective of the process of examining doctoral theses in the creative industries. Susan was central to the process in the Faculty of Creative Industries from 2008 to 2012, and has overseen the carriage of examination for creative arts theses in the creative industries disciplines of creative writing, performance studies, media and communication, journalism, film and television, visual arts, and interaction and visual design.

Modified Lu-Lee cryptosystem

A modified Lu-Lee cryptosystem is proposed which appears to be resistant to the cryptanalytic attacks on the original Lu-Lee scheme. The data expansion due to encryption is moderate, and the size of the public key is also quite small.

Discrete Wiener¿Lee transforms

The use of Wiener–Lee transforms to construct one of the frequency characteristics, magnitude or phase of a network function, when the other characteristic is given graphically, is indicated. This application is useful in finding a realisable network function whose magnitude or phase curve is given. A discrete version of the transform is presented, so that a digital computer can be employed for the computation.

Non-neutral evolution in non-LEE-encoded type III effectors of attaching and effacing Escherichia coli

Attaching and effacing Escherichia coli (AEEC) employ type III secretion system (T3SS) to secrete effector proteins into host cells and regulate their function. Here we have investigated T3SS genes of AEEC for non-neutral evolution. Our analysis revealed non-neutral evolution in three genes (nleE1, nleB2 and nleD) which encode effector proteins. These genes are located outside the locus of enterocyte effacement (LEE). In general, non-LEE effector genes show greater deviation from neutral evolution than LEE effector genes. These results suggest that effector genes located outside LEE are under greater selection pressure than those present in LEE. (C) 2012 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.

Evaluacion del cultivo de sandia (Citrullus lanatus L) variedad Mickey Lee utilizando sustratos mejorados y determinacion de los coeficientes "kc" y ky", bajo riego. Finca Las Mercedes, Managua, 2009

El presente trabajo de investigación se estableció entre los meses de Marzo– Junio 2009, bajo sistema de riego por aspersión en la finca las Mercedes, ubicada en Managua km 11 carretera norte, 2 km entrada al Carnic en las coordenadas 12°10’14” a 12°08’05” de latitud Norte y 86°10’22” a 86°09’44” longitud Oeste, a una altura de 56 msnm. Con el objetivo de evaluar el cultivo de la sandía (Citrullus lanatus L) bajo tres tipos de fertilizantes orgánicos y un sintético, y obtención de los coeficientes del cultivo “Kc” y de rendimiento “Ky”. Se estableció un bloque completo al azar, con cuatro tratamientos y cuatro repeticiones. Los tratamientos orgánicos evaluados son: compost a razón de 25 595 kg ha-1 , bocashi 15 087 kg ha-1 y biofertilizante con aplicaciones de 20 000 lt ha-1 y un tratamiento sintético (testigo): fertilizante completo (NPK) formula 12-30-10, a razón de 350 kg ha-1 más urea 46% a razón de 219 kg ha-1 , todos los abonos orgánicos fueron elaborados como parte de la investigación haciendo uso de los distintos materiales que se tienen en la finca para su elaboración. Los resultados de campos se sometió a un análisis de varianza (ANDEVA) y separación de medias mediante el uso de la prueba de Diferencias Mínimas Significativas (LSD α=0.05). Utilizando el Software estadístico Infostat versión 2008. Con base a los datos obtenidos se puede afirmar, que no hubo diferencia significativa para las variables de crecimiento, tomadas en momentos diferentes, sin embargo para la variable de rendimiento y sus componentes se encontró diferencia significativas en número de frutos por planta (5.13), diámetro del fruto (15.90 cm), peso del fruto (5.96 lb) y rendimiento (70 020 kg ha-1 ) logrando obtener el mejor resultado cuando el cultivo se fertilizó con sintético, Con respecto a las variables de riego con un volumen de agua de 526.5 mm ha-1 en todo el ciclo del cultivo y tomando en cuenta las etapas fenológicas se le aplicó mayor volumen de agua en la etapa de floración y fructificación que es donde el cultivo requiere mayor cantidad de agua, logrando para todos los tratamientos un coeficiente de cultivo uniforme y curva de Kc siguió el mismo patrón de cualquier cultivo comercial, presentando una fase inicial, de desarrollo, de madurez y senescencia; En el caso de la reducción del coeficiente de rendimiento de la sandía en los tratamientos orgánicos no se debe a un estrés hídrico o limitación de agua durante los periodos de floración y fructificación que son los períodos más sensibles a la sequía, mas bien corresponde al factor de fertilidad ya que el abono orgánico no va dirigido a liberar grandes cantidades de nutrientes para el cultivo de una sola vez.

En las raíces ideológicas de Montoneros : John William Cooke lee a Gramsci en Cuba

Desde 1945 la izquierda argentina no pudo resolver el enigma planteado por el hecho de que la clase obrera era peronista. Varios intentos teóricos fracasaron antes de que John William Cooke hiciera el suyo a mediados de los años sesenta. Influenciado por Gramsci, consideró que la clase obrera es una categoría histórica, por lo que el peronismo era la experiencia histórica de la clase obrera argentina y, como tal, revolucionario. Neutralizó el papel político de Perón convirtiéndolo en el símbolo de la identidad peronista. Por esto, la teoría de Cooke fue clave para que guerrilleros de izquierda se declararan peronistas sin que les importaran las opiniones e intenciones políticas de Perón. Las limitaciones de esa teoría quedaron expuestas cuando el general regresó a la Argentina y fue electo presidente por tercera vez.

Saline water intrusion from deep artesian sources in McGregor Isles area of Lee County, Florida

Upward leakage of saline water from an artesian aquifer below 1,500 feet has caused an increase in chloride concentration in the lower Hawthorn aquifer from less than 1,000 mg/1 (milligrams per liter) to values ranging from about 1,300 to 15,000 mg/1. Similarly the higher temperatures of the intruding water has caused an increase in water temperatures in the aquifer from 82"F to values ranging from 83 to 93"F. The intruding water moves upward either through the open bore hole of deep wells or test holes, or along a fault or fracture system, which has been identified in the area. From these points of entry into the lower Hawthorn aquifer, the saline water spreads laterally toward the south and southeast, but is generally confined to components of the fault system. The saline water moves upward from the lower Hawthorn aquifer into the upper Hawthorn aquifer through the open bore hole of wells, which connect the aquifers. This movement has resulted in an increase in chloride from less than 200 mg/1 in the unaffected parts of the upper Hawthorn aquifer to values commonly ranging from about 300 to more than 3,000 mg/1 in parts of the aquifer affected by upward leakage. The upper Hawthorn aquifer is the principal source of ground-water supply for public water-supply systems in western Lee County. Similar effects have been noted in the water-table aquifer, where chloride increased from less than 100 to concentrations ranging from about 500 to more than 5,000 mg/1. This was caused by the downward infiltration of water discharged at land surface from wells tapping the lower Hawthorn aquifer. The spread of saline water throughout most of the McGregor Isles area is continuing as of 1971. (40 page document)

Interim report on surface water resources and quality of waters in Lee County, Florida

Report seeks to address following questions: 1. Where within Lee County are surface supplies of water located? 2. What are the variations in this supply? 3. What can be done to provide better answers to questions 1 and 2 than are available at the present time? (PDF contains 76 pages.)

¿Quién lee las estadísticas oficiales?

Duración (en horas): De 41 a 50 horas. Destinatario: Estudiante y Docente