O SLA funk de Fernanda Abreu

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Labirintos da identidade: de como um fidalgo castelhano-manchego foi chamado a salvar a pátria portuguesa.(da literatura comparada ao fado lusíada)

pp. 187-199

Extração, estruturas e propriedades de alfa- e beta-quitina

The fact that alpha- and beta-chitin adopt different arrays in the solid state is explored to emphasize their different properties and distinct spectral characteristics and X ray diffraction patterns. The methods for their extraction from the biomass in view of the preservation of their native structures and aiming to fulfill the claims of purity and uniformity for potential applications are discussed. The different arrays adopted by alpha- and beta-chitin also result in distinct reactivities toward the deacetylation reaction. Thus, the deacetylation of beta-chitin is more efficient owing to the better accessibility to amide groups due to the lower crystallinity of this polymorph.

Thermodynamic and statistical analysis of soybean oil extraction process using renewable solvent

The main goal of this work was to evaluate thermodynamic parameters of the soybean oil extraction process using ethanol as solvent. The experimental treatments were as follows: aqueous solvents with water contents varying from 0 to 13% (mass basis) and extraction temperature varying from 50 to 100 degrees C. The distribution coefficients of oil at equilibrium have been used to calculate enthalpy, entropy and free energy changes. The results indicate that oil extraction process with ethanol is feasible and spontaneous, mainly under higher temperature. Also, the influence of water level in the solvent and temperature were analysed using the response surface methodology (RSM). It can be noted that the extraction yield was highly affected by both independent variables. A joint analysis of thermodynamic and RSM indicates the optimal level of solvent hydration and temperature to perform the extraction process.

Characteristics and properties of carboxymethylchitosan

Four samples of N,O-carboxymethylchitosan (0.5 < <(DS)over bar> < 1.5) were prepared by reacting chitosan (<(DA)over bar> = 24%) with monochloroacetic acid in the presence of excess sodium hydroxide. The carboxymethylchitosan samples were soluble in a wider range of pH as compared to the parent chitosan and the X-ray diffraction showed that they adopt a less ordered arrangement. The carboxymethylation of chitosan decreased the thermal stability of the polymer as evaluated by thermogravimetry but no clear dependence of the activation energy on the average degree of substitution of carboxymethylchitosan was identified. However, the values of activation energy of carboxymethylchitosan issued from the isothermal study depended on the degree of conversion, suggesting the occurrence of a complex set of simultaneous reactions. (C) 2008 Published by Elsevier Ltd.

Purification and characterization of a surfactin-like molecule produced by Bacillus sp. H2O-1 and its antagonistic effect against sulfate reducing bacteria

Abstract Background Bacillus sp. H2O-1, isolated from the connate water of a Brazilian reservoir, produces an antimicrobial substance (denoted as AMS H2O-1) that is active against sulfate reducing bacteria, which are the major bacterial group responsible for biogenic souring and biocorrosion in petroleum reservoirs. Thus, the use of AMS H2O-1 for sulfate reducing bacteria control in the petroleum industry is a promising alternative to chemical biocides. However, prior to the large-scale production of AMS H2O-1 for industrial applications, its chemical structure must be elucidated. This study also analyzed the changes in the wetting properties of different surfaces conditioned with AMS H2O-1 and demonstrated the effect of AMS H2O-1 on sulfate reducing bacteria cells. Results A lipopeptide mixture from AMS H2O-1 was partially purified on a silica gel column and identified via mass spectrometry (ESI-MS). It comprises four major components that range in size from 1007 to 1049 Da. The lipid moiety contains linear and branched β-hydroxy fatty acids that range in length from C13 to C16. The peptide moiety contains seven amino acids identified as Glu-Leu-Leu-Val-Asp-Leu-Leu. Transmission electron microscopy revealed cell membrane alteration of sulfate reducing bacteria after AMS H2O-1 treatment at the minimum inhibitory concentration (5 μg/ml). Cytoplasmic electron dense inclusions were observed in treated cells but not in untreated cells. AMS H2O-1 enhanced the osmosis of sulfate reducing bacteria cells and caused the leakage of the intracellular contents. In addition, contact angle measurements indicated that different surfaces conditioned by AMS H2O-1 were less hydrophobic and more electron-donor than untreated surfaces. Conclusion AMS H2O-1 is a mixture of four surfactin-like homologues, and its biocidal activity and surfactant properties suggest that this compound may be a good candidate for sulfate reducing bacteria control. Thus, it is a potential alternative to the chemical biocides or surface coating agents currently used to prevent SRB growth in petroleum industries.

A lectoescrita em alunos surdos a frequentar o ensino bilingue: avaliação da eficácia de um programa de treino

As crianças surdas, integradas num contexto educativo bilingue, aprendem a ler e a escrever numa língua oral-auditiva, língua com caraterísticas diferentes da língua que adquirem naturalmente que é visuo-espacial. A Língua Gestual Portuguesa é a língua materna dos surdos, constituindo-se a Língua Portuguesa, no modo oral e escrito, como a sua segunda língua. Face à escassez de instrumentos facilitadores do desenvolvimento das competências de leitura e escrita em crianças surdas, esta investigação visa avaliar a eficácia do programa de treino da competência lectoescrita para alunos surdos a frequentar o ensino bilingue – Programa PODER. O programa de intervenção concebido desenvolveu-se num processo de investigação-ação e foi aplicado a seis alunos surdos, a frequentar o 2.º ano de escolaridade do ensino básico, numa Escola de Referência para a Educação Bilingue de Alunos Surdos. As respostas obtidas, através de questionários aos alunos surdos (6), foram analisadas qualitativamente. Para medir o índice de legibilidade do texto e a facilidade de leitura, no Pré-teste e no Pós-teste de lectoescrita aplicado aos alunos surdos, utilizou-se o “Índice de Legibilidade de FleschKincaid Grade Level” e o “Índice de Facilidade de Leitura de Flesch” (ambos baseados no comprimento das palavras e das frases). Os resultados obtidos indicam que o Índice de Legibilidade fica situado entre 7,0 e 10,0, o recomendável e o Índice de Facilidade de Leitura enquadra-se no Nível A1 – Utilizador Básico. Os dados afiguram-se expressivos para a validação do programa de treino e prova da sua eficácia, no desenvolvimento da competência lectoescrita de alunos surdos integrados no ensino bilingue. Deste estudo, retiram-se implicações para a intervenção psicoeducacional da lectoescrita junto de alunos surdos que frequentam o ensino bilingue.

A lectoescrita em alunos surdos a frequentar o ensino bilingue: avaliação da eficácia de um programa de treino

As crianças surdas, integradas num contexto educativo bilingue, aprendem a ler e a escrever numa língua oral-auditiva, língua com caraterísticas diferentes da língua que adquirem naturalmente que é visuo-espacial. A Língua Gestual Portuguesa é a língua materna dos surdos, constituindo-se a Língua Portuguesa, no modo oral e escrito, como a sua segunda língua. Face à escassez de instrumentos facilitadores do desenvolvimento das competências de leitura e escrita em crianças surdas, esta investigação visa avaliar a eficácia do programa de treino da competência lectoescrita para alunos surdos a frequentar o ensino bilingue – Programa PODER. O programa de intervenção concebido desenvolveu-se num processo de investigação-ação e foi aplicado a seis alunos surdos, a frequentar o 2.º ano de escolaridade do ensino básico, numa Escola de Referência para a Educação Bilingue de Alunos Surdos. As respostas obtidas, através de questionários aos alunos surdos (6), foram analisadas qualitativamente. Para medir o índice de legibilidade do texto e a facilidade de leitura, no Pré-teste e no Pós-teste de lectoescrita aplicado aos alunos surdos, utilizou-se o “Índice de Legibilidade de FleschKincaid Grade Level” e o “Índice de Facilidade de Leitura de Flesch” (ambos baseados no comprimento das palavras e das frases). Os resultados obtidos indicam que o Índice de Legibilidade fica situado entre 7,0 e 10,0, o recomendável e o Índice de Facilidade de Leitura enquadra-se no Nível A1 – Utilizador Básico. Os dados afiguram-se expressivos para a validação do programa de treino e prova da sua eficácia, no desenvolvimento da competência lectoescrita de alunos surdos integrados no ensino bilingue. Deste estudo, retiram-se implicações para a intervenção psicoeducacional da lectoescrita junto de alunos surdos que frequentam o ensino bilingue.

Letters that construct limits: intellectual and political projects in the letters of Capistrano de Abreu to Barao do Rio Branco (1886-1903)

The intention of this paper is to analyze the letters from Capistrano de Abreu to Barao do Rio Branco in the years between 1886 and 1903. The focus will be given to the divergences around the notion of territorial formation, a basic concept for these authors who were thinking about the construction of a historical narrative at the end of the 19(th) and beginning of the 20(th) century. Later, the question is the construction of the craft of the historian in the letters of Capistrano de Abreu and his distinction and proximity to the ideas of the Barao do Rio Branco.

Effects of bone marrow-derived mononuclear cells on airway and lung parenchyma remodeling in a murine model of chronic allergic inflammation

We hypothesized that bone marrow-derived mononuclear cells (BMDMC) would attenuate the remodeling process in a chronic allergic inflammation model. C57BL/6 mice were assigned to two groups. In OVA, mice were sensitized and repeatedly challenged with ovalbumin. Control mice (C) received saline under the same protocol. C and OVA were further randomized to receive BMDMC (2 x 10(6)) or saline intravenously 24 h before the first challenge. BMDMC therapy reduced eosinophil infiltration, smooth muscle-specific actin expression, subepithelial fibrosis, and myocyte hypertrophy and hyperplasia, thus causing a decrease in airway hyperresponsiveness and lung mechanical parameters. BMDMC from green fluorescent protein (GFP)-transgenic mice transplanted into GFP-negative mice yielded lower engraftment in OVA. BMDMC increased insulin-like growth factor expression, but reduced interleukin-5, transforming growth factor-beta, platelet-derived growth factor, and vascular endothelial growth factor mRNA expression. In conclusion, in the present chronic allergic inflammation model, BMDMC therapy was an effective pre-treatment protocol that potentiated airway epithelial cell repair and prevented inflammatory and remodeling processes. (C) 2010 Elsevier B.V. All rights reserved.

Hypervolemia induces and potentiates lung damage after recruitment maneuver in a model of sepsis-induced acute lung injury

Introduction: Recruitment maneuvers (RMs) seem to be more effective in extrapulmonary acute lung injury (ALI), caused mainly by sepsis, than in pulmonary ALI. Nevertheless, the maintenance of adequate volemic status is particularly challenging in sepsis. Since the interaction between volemic status and RMs is not well established, we investigated the effects of RMs on lung and distal organs in the presence of hypovolemia, normovolemia, and hypervolemia in a model of extrapulmonary lung injury induced by sepsis. Methods: ALI was induced by cecal ligation and puncture surgery in 66 Wistar rats. After 48 h, animals were anesthetized, mechanically ventilated and randomly assigned to 3 volemic status (n = 22/group): 1) hypovolemia induced by blood drainage at mean arterial pressure (MAP)approximate to 70 mmHg; 2) normovolemia (MAP approximate to 100 mmHg), and 3) hypervolemia with colloid administration to achieve a MAP approximate to 130 mmHg. In each group, animals were further randomized to be recruited (CPAP = 40 cm H(2)O for 40 s) or not (NR) (n = 11/group), followed by 1 h of protective mechanical ventilation. Echocardiography, arterial blood gases, static lung elastance (Est, L), histology (light and electron microscopy), lung wet-to-dry (W/D) ratio, interleukin (IL)-6, IL-1 beta, caspase-3, type III procollagen (PCIII), intercellular adhesion molecule-1 (ICAM-1), and vascular cell adhesion molecule-1 (VCAM-1) mRNA expressions in lung tissue, as well as lung and distal organ epithelial cell apoptosis were analyzed. Results: We observed that: 1) hypervolemia increased lung W/D ratio with impairment of oxygenation and Est, L, and was associated with alveolar and endothelial cell damage and increased IL-6, VCAM-1, and ICAM-1 mRNA expressions; and 2) RM reduced alveolar collapse independent of volemic status. In hypervolemic animals, RM improved oxygenation above the levels observed with the use of positive-end expiratory pressure (PEEP), but increased lung injury and led to higher inflammatory and fibrogenetic responses. Conclusions: Volemic status should be taken into account during RMs, since in this sepsis-induced ALI model hypervolemia promoted and potentiated lung injury compared to hypo-and normovolemia.

"Procedural Strategy" by Carlos Pinto de Abreu, lawyer

Como Carlos Pinto de Abreu refere e bem, no seu livro e no seminário, entre juízes, partes ou sujeitos processuais, advogados ou solicitadores, magistrados do Ministério Público ou testemunhas, peritos ou funcionários, encontramos “pessoas que podem ser mais ou menos sérias”, por sinal “motivadas por outros interesses que não os de servir a Verdade e a Justiça”; Abstract: Carlos Pinto de Abreu as regards and well in his book and seminar, between judges, parties or procedural subject, lawyers or solicitors, prosecutors and witnesses, experts or officials, found "people who may be more or less serious" , by the way "motivated by interests other than those serving the Truth and Justice".

Literatura, constructos cosmovisionarios y conflictos sociales : "la shoá" y "la violencia" Laura Restrepo y George steiner: aproximaciones desde la pragmática de la productividad realista intencional

Trabalho de Projecto de Mestrado em: As Humanidades na Europa: Convergências e Perspectivas. CROSSWAYS IN EUROPEAN HUMANITIES