Foraminíferos, ostracodes e microfauna associada da plataforma continental equatorial norte-rio-grandense, NE Brasil: área Porto do Mangue a Galinhos

This study was conducted in the adjacent Brazilian equatorial inner shelf to Rio Grande do Norte, between the region of Porto do Mangue and Galinhos. The main objective is the characterization of biogenic sediments, especially foraminifera and ostracod collected on the surface of the seafloor. The methodology involved standard procedures including literature, surveys, processing of samples in the laboratory and identification of foraminifera and ostracods by genera or species under stereo microscopy and scanning electron microscopy (SEM). Multivariate statistical analyzes and study of ecological indexes were also applied to the study of foraminifera. Three transects, from inner shelf to slope were sampled: profile 01 (east, near Galos), profile 02 (center, near the city of Macau) and profile 03 (west, near Ponta do Mel). Results indicated the predominance of benthic foraminifera and little plankton occurrence. Benthic foraminifera genera observed in abundance were Quinqueloculina, Textularia, Globigerina and Pyrgo, Quinqueloculina, Textularia, Pyrgo, Ammonia, Elphidium, Pseudononion, Peneroplis, Bolivina and Poroeponides, occurred more frequently. Less frequently been described Amphistegina, Archaias, Bigenerina, Cibicides, Cassidulina, Amphicorina, Cornuspira, Paterina, Hopkunsina, Oolina, Uvigerina, Fusenkoina, Nonionella, Amphisorus, Wiesrella, Reussella, Reophax, Nodosaria, Marginulina and Cyclogyra. Six genera of ostracods were also identified: Puriana variabilis / P. convoluted?, Loxoconcha sp, Bairdiidae, Xestoleberis sp, Hemicytheridae and Ruggiericythere sp. Groups of organisms found in the studied shelf presented chemical composition of Ca, C, O, Na, Cl, Al, Mg, and Si. The proportions of chemical elements may vary according to the type of biogenic sediment, with the highest values identified as Ca, C, Cl, Na and O. The absolute dating by carbon 14 method indicated sediments of different colors (light and dark), correspond to a single age from 3000 to 6000 years BP, related to the Quaternary. These data intend to complement information about biogenic sediments in the Brazilian continental shelf, especially in the Northeast, where there is a lack of such studies.

Weaving Futures. Family Transnationalism in the migration from Dominican Republic and Brazil to Spain

This article explores forms of migrant families’ reorganization within a (new) global economic crisis and the hardening of migration control in Europe; based on the cases of Dominican and Brazilian migration to Spain.Our goal is not to characterize the wholeness of strategies from these collectives, instead visualize its heterogeneity. Displacement of Dominican and Brazilian population to Spain shares the role of women as the first link of migration chains. In both cases women are the economic support of transnational families and they lead reunification's processes. Nevertheless, differences in the time spent in the destination country, migratory status, origin (rural-urban), level of education, class and labor insertion in destination country, affect differently, the planning and start up of migration projects, the organization of care and family reunification strategies. These findings question the predominant place granted to national origin in the study of international migration.

Do Institutional Participatory Projects Contribute to the Development of Political Capabilities?

Participation usually sets off from the bottom up, taking the form of more or less enduring forms of collective action with varying degrees of infl uence. However, a number of projects have been launched by political institutions in the last decades with a view to engaging citizens in public affairs and developing their democratic habits, as well as those of the administration. This paper analyses the political qualifying capacity of the said projects, i.e. whether participating in them qualifi es individuals to behave as active citizens; whether these projects foster greater orientation towards public matters, intensify (or create) political will, and provide the necessary skills and expertise to master this will. To answer these questions, data from the comparative analysis of fi ve participatory projects in France and Spain are used, shedding light on which features of these participatory projects contribute to the formation of political subjects and in which way. Finally, in order to better understand this formative dimension, the formative capacity of institutional projects is compared with the formative dimension of other forms of participation spontaneously developed by citizens.

El autocuidado y apoyo social de mujeres de un proyecto de seguimiento mamográfico en Porto Alegre/Brasil

Objective: Identify preventive self-care practices and analyze the configurations of the network support for women with and without breast cancer registered in a mammography-monitoring project from Porto Alegre/Brazil.Method: a mixed sequential delimitation was performed, which expanded the results of the quantitative step (cross and correlation section) in a qualitative step (narrative interviews). 37 women diagnosed with breast cancer (group 1) and 72 without this diagnosis (group 2 – monitoring) participated. The following instruments were used: Assessment Questionnaire Self-care Ability (ASA-A) and Assessment Questionnaire Perceived Social Support and Community. There were performed descriptive analysis and comparison of means (t test and ANOVA) between the two groups. To deepen the understanding of the data, we selected four women with breast cancer with extreme levels on the scale of Social Support to participate in the biographical narrative interviews.Results: the analysis indicate that women who had breast cancer have better self-care practices than the women from the monitoring project (t = 1.791, P = 0.027). As for the analysis of social support, there were no statistically significant differences between the two groups. All participants have an average level of perceived social and community support. It was highlighted by the qualitative data that it was after the diagnosis of breast cancer that women lived self-care aspects they had not previously experienced.Conclusions: the self-care was significantly bigger in the group of women with breast cancer, where the cancer diagnosis was a trigger to increase self-care.

Os Deputados Assistentes Sociais na Assembleia Nacional e na Assembleia da República Portuguesa: principais intervenções parlamentares entre 1949 e 2011

A presente dissertação é relativa aos assistentes sociais que exerceram funções de deputados em Portugal Continental, na Assembleia Nacional e na Assembleia da República Portuguesa. Procedeu-se à sua caracterização e à análise dos seus principais discursos parlamentares. A pertinência da investigação é justificada pela inexistência de trabalhos acerca da temática escolhida e pela reduzida produção académica no que respeita à vasta dimensão política do Serviço Social em Portugal. A investigação alicerçou-se numa pesquisa bibliográfica sobre os temas em foco, e documental, na análise das fichas biográficas dos deputados identificados e das transcrições textuais das sessões da Assembleia Nacional e da Assembleia da República Portuguesa, onde constam os discursos destes deputados. Estas fontes primárias estão acessíveis no sítio oficial da Assembleia da República (www.parlamento.pt). Pretende-se com este estudo abordar o lugar atribuído à Assembleia Nacional e à Assembleia da República Portuguesa nos respetivos regimes de ditadura e democracia e identificar e caracterizar académica, profissional e politicamente os assistentes sociais que exerceram estas funções. Objetiva-se ainda a análise das principais intervenções destes deputados no contexto da Assembleia Nacional e da Assembleia da República Portuguesa. Apurou-se que o número de deputadas à Assembleia Nacional e à Assembleia da República Portuguesa não variou significativamente nos regimes políticos abordados. Foram identificadas cinco deputadas à Assembleia Nacional, da V à XI legislatura (de 1949 a 1974 – 25 anos), sendo que a primeira deputada identificada exerceu funções ao longo da V e VI legislaturas da Assembleia Nacional. Após a queda do regime ditatorial e depois de 21 anos em democracia, assistentes sociais voltaram a exercer funções de deputados. Entre 1995 e 2011 exerceram funções seis deputados à Assembleia da República Portuguesa. Um deles desenvolveu atividades ao longo da X e XI legislaturas. Os resultados da investigação mostram que a maioria destes deputados são mulheres, havendo apenas um homem. Relativamente à formação académica dos deputados constatou-se que antes de 1974 o estabelecimento que formou mais assistentes sociais deputadas foi o Instituto de Serviço Social de Lisboa (3), seguido do Instituto Superior de Ciências Sociais e Políticas Ultramarinas (1) e do Instituto Superior de Serviço Social Pio XII, em Luanda (1). Os deputados à Assembleia da República formaram-se no Instituto Superior de Serviço Social de Coimbra (2); no Instituto Superior Bissaya Barreto, Coimbra (1); no Instituto Superior de Serviço Social do Porto (1); no Instituto Superior de Serviço Social de Lisboa (1) e no Instituto Superior de Serviço Social Pio XII, Luanda (1). As deputadas que exerceram funções durante o Estado Novo pertenciam à União Nacional e Acção Nacional Popular, sendo que uma delas pertencia à Ala Liberal da ANP. Quanto aos deputados que exerceram funções no período democrático percebe-se que todos eram vinculados a partidos do bloco central e da direita: uma deputada do PSD, uma do CDS e quatro deputados do PS. No que respeita às intervenções parlamentares dos deputados identificados, observou-se que as deputadas à Assembleia Nacional apresentaram e fundamentaram propostas de medidas de política social apoiando-se na sua experiência profissional, principalmente em debates em que o tema foi a família ou a pobreza. Os deputados à Assembleia da República direcionaram os seus discursos para o apoio e/ou complemento das iniciativas propostas pelo governo, pois quatro destes deputados exerceram funções enquanto o partido a que pertenciam estava na liderança do governo. Face á vastidão da temática abordada, a presente dissertação constitui-se como um primeiro contributo para a produção de bibliografia no âmbito da dimensão política do Serviço Social, reconhecendo-se a necessidade de aprofundar mais o tema. / This thesis is on social workers who acted as deputies in Portugal, on the National Assembly and the Assembly of the Portuguese Republic. Characterization and analysis of its key parliamentary speeches was the chosen procedure. The relevance of the research is justified by the lack of previous work on the chosen theme and the reduced academic outputs with respect to the vast political dimension of social work in Portugal. Research has its foundations in a literature research on the topics in focus, and also documentary, by the analysis of the biographical records of Members discussed and the verbatim transcripts of the sessions of the National Assembly and the Assembly of the Portuguese Republic, which contains the speeches of such Members. These primary sources include legislative sessions and are available on the Assembly of the Republic (www.parlamento.pt) official website. The study aims to address the place assigned to the National Assembly and the Assembly of the Portuguese Republic, regarding the respective regimes of dictatorship and democracy and to identify and characterize academically, professionally and politically, the social workers who have exercised these functions. Furthermore on the objective is the analysis of the main interventions in the context of such Members of the National Assembly and the Assembly of the Portuguese Republic. It was found that the number of deputies to the National Assembly and the Assembly of the Portuguese Republic did not significantly vary in both political regimes. Five deputies to the National Assembly were identified on the legislatures V to XI (1949-1974 - 25 years), with the first identified lady holding positions along the V and VI legislatures of the National Assembly. After the fall of the dictatorship and after 21 years of democracy, social workers returned to act as deputies. Between 1995 and 2011 held office six members of the Assembly of the Portuguese Republic. One developed activities along the X and XI legislatures of the Assembly of the Portuguese Republic. Research results show that the majority of these are women, and only one man. Regarding the academic training of the deputies, it was found that before 1974 the establishment that formed most of the social workers was, the Institute of Social Service of Lisbon (3), followed by the School of Social Science and Policy Overseas (1) and the Higher Institute of Social Service Pius XII in Luanda (1). Members of Parliament formed in the Institute of Social Service of Coimbra (2), the Higher Institute Bissaya Barreto, Coimbra (1), the Institute of Social Service of Porto (1), the Higher Institute of Social service of Lisbon (1) and the Institute of Social service Pius XII, Luanda(1). Deputies who held office during the New state belonged to the National Union and National People's Action parties, one of which belonged to the Liberal Wing of the ANP. As for the deputies who held office in the democratic period one realizes that all parties were bound to the central block and right wing: A Member of the PSD, one of CDS and four members of the PS. Regarding the parliamentary speeches of identified Members, it was noted that the National Assembly deputies presented and substantiated proposals for social policy measures relying on their professional experience, primarily on the debates in which the theme was family or poverty. Members of Parliament have directed their speeches to support and / or complement the initiatives proposed by the government, since four of these deputies exercise their functions while the party they belonged was in governmental leadership. Given the vastness of the topic addressed in this thesis, it constitutes itself as a first contribution to the production of literature within the political dimension of social work, recognizing the need to go deeper into the subject.

Ação pública na formação e transformação do destino turístico de Porto de Galinhas: um estudo dos referenciais no período de 1970 a 2010

The central interest of this thesis is to comprehend how the public action impels the formation and transformation of the tourist destinies. The research was based on the premise that the public actions are the result of the mediation process of state and non-state actors considered important in a section, which interact aiming for prevailing their interests and world visions above the others. The case of Porto de Galinhas beach, in Pernambuco, locus of the investigation of this thesis, allowed the analysis of a multiplicity of actors on the formation and implementation of local actions toward the development of the tourism between the years 1970 and 2010, as well as permitted the comprehension of the construction of the referential on the interventions made. This thesis, of a qualitative nature, has as theoretical support the cognitive approach of analysis of the public policies developed in France, and it has as main exponents the authors Bruno Jobert and Pierre Muller. This choice was made by the emphasis on the cognitive and normative factors of the politics, which aspects are not very explored in the studies of public policies in Brazil. As the source of the data collection, documental, bibliographic and field researches were utilized to the (re)constitution of the formation and transformation in the site concerned. The analysis techniques applied were the content and the documental analysis. To trace the public action referential, it started by the characterization of the touristic section frontiers and the creation of images by the main international body: the World Tourism Organization, of which analysis of the minutes of the meetings underscored guidelines to the member countries, including Brazil, which compounds the global-sectorial reference of the section. As from the analysis of the evolution of the tourism in the country, was identified that public policies in Brazil passed by transformations in their organization over the years, indicating changes in the referential that guided the interventions. These guidelines and transformations were identified in the construction of the tourist destination of Porto de Galinhas, of which data was systematized and presented in four historical periods, in which were discussed the values, the standard, the algorithms, the images and the important mediators. It has been revealed that the State worked in different roles in the decades analyzed in local tourism. From the 1990s, however, new actors were inserted in the formulation and implementation of policies developed, especially for local hotelkeepers. These, through their association, establishes a leadership relation in the local touristic section, thereby, they could set their hegemony and spread their own interest. The leadership acquired by a group of actors, in the case of Porto de Galinhas, does not mean that trade within the industry were neutralized, but that there is a cognitive framework that confronts the actors involved. In spite of the advances achieved by the work of the mediators in the last decades, that resulted in an amplification and diversification of the activity in the area, as well as the consolidation at the beach, as a tourist destiny of national standout, the position of the place is instable, concerned to the competitiveness, once that there is an situation of social and environmental unsustainability

Ação pública na formação e transformação do destino turístico de Porto de Galinhas: um estudo dos referenciais no período de 1970 a 2010

The central interest of this thesis is to comprehend how the public action impels the formation and transformation of the tourist destinies. The research was based on the premise that the public actions are the result of the mediation process of state and non-state actors considered important in a section, which interact aiming for prevailing their interests and world visions above the others. The case of Porto de Galinhas beach, in Pernambuco, locus of the investigation of this thesis, allowed the analysis of a multiplicity of actors on the formation and implementation of local actions toward the development of the tourism between the years 1970 and 2010, as well as permitted the comprehension of the construction of the referential on the interventions made. This thesis, of a qualitative nature, has as theoretical support the cognitive approach of analysis of the public policies developed in France, and it has as main exponents the authors Bruno Jobert and Pierre Muller. This choice was made by the emphasis on the cognitive and normative factors of the politics, which aspects are not very explored in the studies of public policies in Brazil. As the source of the data collection, documental, bibliographic and field researches were utilized to the (re)constitution of the formation and transformation in the site concerned. The analysis techniques applied were the content and the documental analysis. To trace the public action referential, it started by the characterization of the touristic section frontiers and the creation of images by the main international body: the World Tourism Organization, of which analysis of the minutes of the meetings underscored guidelines to the member countries, including Brazil, which compounds the global-sectorial reference of the section. As from the analysis of the evolution of the tourism in the country, was identified that public policies in Brazil passed by transformations in their organization over the years, indicating changes in the referential that guided the interventions. These guidelines and transformations were identified in the construction of the tourist destination of Porto de Galinhas, of which data was systematized and presented in four historical periods, in which were discussed the values, the standard, the algorithms, the images and the important mediators. It has been revealed that the State worked in different roles in the decades analyzed in local tourism. From the 1990s, however, new actors were inserted in the formulation and implementation of policies developed, especially for local hotelkeepers. These, through their association, establishes a leadership relation in the local touristic section, thereby, they could set their hegemony and spread their own interest. The leadership acquired by a group of actors, in the case of Porto de Galinhas, does not mean that trade within the industry were neutralized, but that there is a cognitive framework that confronts the actors involved. In spite of the advances achieved by the work of the mediators in the last decades, that resulted in an amplification and diversification of the activity in the area, as well as the consolidation at the beach, as a tourist destiny of national standout, the position of the place is instable, concerned to the competitiveness, once that there is an situation of social and environmental unsustainability

Primeiras aproximações de uma análise do financiamento da política nacional de esporte e lazer no Governo Lula

Este artigo realiza uma análise introdutória sobre o gasto público referente às políticas de esporte e lazer no Governo Lula. Os dados coletados apresentaram uma distribuição instável entre os programas, o que impede o estabelecimento de um padrão de financiamento para o esporte e lazer durante o período analisado (2003-2010), bem como de afirmar a existência de programas e/ou frações de classes hegemônicas no que tange aos gastos públicos do setor. Todavia, algumas particularidades indicam a razoabilidade do pressuposto sobre a priorização do esporte de alto rendimento dentro da alocação dos recursos públicos destinados ao financiamento da Política Nacional de Esporte e Lazer (PNEL) do Governo Lula. __________________________________________________________________________________________________ ABSTRACT

Burnout em profissionais da saúde portugueses: Uma análise a nível nacional

Introdução: O burnout é uma síndrome psicológica, caracterizada por elevada exaustão emocional, elevada despersonalização e baixa realização profissional, que conduz à erosão dos valores pessoais, profissionais e de saúde. Este estudo reporta a prevalência do burnout em profissionais de saúde Portugueses. Material e Métodos: Os níveis de burnout foram estimados pelo Maslach Burnout Inventory - Human Services Survey numa escala ordinal de zero (nunca) a seis (sempre) pontos. A amostra foi constituída por 1 262 enfermeiros e 466 médicos com médias de idade de 36,8 anos (DP = 12,2) e 38,7 (DP = 11,0), respetivamente. Os participantes foram provenientes de todos os distritos nacionais (35% Lisboa; 18% Porto; 6% Aveiro, 6% Setúbal, 5% Coimbra; 5% regiões autónomas), com atuação em meio hospitalar (54%), centros de saúde (Unidade de Saúde Familiar - 30%; Unidades de Cuidados de Saúde Primários - 8%) e outras instituições públicas/privadas (8%). Resultados: A análise dos níveis de burnout revelou que ambas as categorias profissionais apresentaram níveis moderados a elevados de burnout (M = 3,0; DP = 1,7) não sendo significativas as diferenças entre as duas profissões. Vila Real (M = 3,8; SD = 1,7) e a Madeira (M = 2,5; DP = 1,5) são as regiões onde os níveis de burnout são mais e menos elevados, respetivamente. Os níveis de burnout não diferiram significativamente entre Hospitais, Unidades de Cuidados de Saúde Personalizados e Unidades de Saúde Familiares. Os profissionais com maior tempo na função são menos acometidos por burnout (r = -0,15) não ocorrendo associação significativa com a duração da jornada de trabalho (r = 0,04). A má qualidade das condições de trabalho foi o melhor preditor do burnout (r = -0,35). Discussão: A ocorrência da síndrome de burnout em profissionais de saúde portugueses é frequente, estando associada à percepção de más condições de trabalho e à menor duração do tempo de serviço. A incidência de burnout apresenta diferenças regionais que podem estar associadas ao aumento do stress imposto pelo exercício da profissão em condições sub-ótimas para a prestação dos cuidados de saúde. Os resultados alertam para a necessidade de intervenções para melhorar as condições de trabalho e formação inicial dos profissionais de saúde de forma a garantir a qualidade do serviço prestado aos utentes e o bem-estar pessoal destes profissionais. Conclusões: A nível nacional, entre 2011 e 2013, 21,6% dos profissionais de saúde apresentaram burnout moderado e 47,8% burnout elevado. A perceção de más condições de trabalho foi o principal preditor da ocorrência de burnout nos profissionais de saúde Portugueses.

IMPLANTAÇÃO DAS AÇÕES DA POLÍTICA NACIONAL DE ATENÇÃO INTEGRAL À SAÚDE DO HOMEM NO MUNICÍPIO DE PORTO MURTINHO - MS

Pesquisas apontam que o número de mortes por violência ou acidentes com homens é maior quando comparado com mulheres nas mesmas circunstâncias. A população masculina não busca os serviços de atenção primária, iniciando seu atendimento em saúde através da atenção ambulatorial e hospitalar, tanto de média quanto de alta complexidade, acarretando em agravos da morbidade e consequentemente gerando maiores gastos para o sistema de saúde. Para tanto, o Ministério da Saúde cria a Política Nacional de Atenção Integral à Saúde do Homem. O objetivo deste projeto de intervenção foi organizar o acesso da população masculina na faixa etária de 20 a 59 anos às ações e serviços de saúde melhorando as condições de saúde desta clientela na cidade de Porto Murtinho-MS. Em Porto Murtinho um cronograma de ações foi desenvolvido e apresentado para a apreciação do secretário municipal de saúde e do prefeito. Após aprovado passou a ser incorporado na dotação orçamentária. O planejamento estava alinhado com o índice de morbimortalidade do município, contemplando educação em saúde nas doenças cardiológicas, metabólicas, doenças sexualmente transmissíveis, AIDS. Colocar em prática uma política desenvolvida e pensada para os homens não é tarefa das mais fáceis. Entraves burocráticos e a dificuldade de obtenção de recursos financeiros se mostram como barreiras a serem superadas com planejamento e colaboração dos envolvidos.

Prevalence And Treatment Of Hypertension In Urban And Riverside Areas In Porto Velho, The Brazilian Amazon.

Abstract Introduction: Hypertension (HTN) is a preventable cause of cardiovascular morbidity and mortality. To compare the prevalence, awareness, treatment, and control of HTN among urban and riverside populations in Porto Velho, Amazon region. We conducted a cross-sectional study between July and December 2013 based on a household survey of individuals aged 35-80 years. Interviews by using a standardized questionnaire, and blood pressure (BP), weight, height, and waist circumference measurements were performed. HTN was defined when individuals reported having the disease, received antihypertensive medications, or had a systolic BP ≥ 140 mm Hg or diastolic BP ≥ 90 mm Hg. Awareness was based on self-reports and the use of antihypertensive medications. Control was defined as a BP ≤ 140/90 mm Hg. Among the 1410 participants, 750 (53.19%) had HTN and 473 (63.06%) had diagnosis awareness, of whom 404 (85.41%) received pharmacological treatment but with low control rate. The prevalence and treatment rates were higher in the urban areas (55.48% vs. 48.87% [p = 0.02] and 61.25% vs. 52.30% [p < 0.01], respectively). HTN awareness was higher in the riverside area (61.05% vs. 67.36% ; p < 0.01), but the control rates showed no statistically significant difference (22.11% vs. 23.43% ; p = 0.69). HTN prevalence was higher in the urban population than in the riverside population. Of the hypertensive individuals in both areas, <25% had controlled HTN. Comprehensive public health measures are needed to improve the prevention and treatment of systemic arterial HTN and prevent other cardiovascular diseases.

Brazilian multicentre study of common mental disorders in primary care: rates and related social and demographic factors

Mental health problems are common in primary health care, particularly anxiety and depression. This study aims to estimate the prevalence of common mental disorders and their associations with socio-demographic characteristics in primary care in Brazil (Family Health Strategy). It involved a multicenter cross-sectional study with patients from Rio de Janeiro, São Paulo, Fortaleza (Ceará State) and Porto Alegre (Rio Grande do Sul State), assessed using the General Health Questionnaire (GHQ-12) and the Hospital Anxiety and Depression Scale (HAD). The rate of mental disorders in patients from Rio de Janeiro, São Paulo, Fortaleza and Porto Alegre were found to be, respectively, 51.9%, 53.3%, 64.3% and 57.7% with significant differences between Porto Alegre and Fortaleza compared to Rio de Janeiro after adjusting for confounders. Prevalence proportions of mental problems were especially common for females, the unemployed, those with less education and those with lower incomes. In the context of the Brazilian government's moves towards developing primary health care and reorganizing mental health policies it is relevant to consider common mental disorders as a priority alongside other chronic health conditions.

Sistema Nacional de Educação articulado ao Plano Nacional de Educação

This text which discusses the central theme of the National Conference on Education (CONAE), held in Brasília from 28th March to 1st April 2010, deals with the concept of a National System of Education in articulation with the National Plan of Education. To that end, after pointing to the basic uses of the concept of system, it discusses the question of the National System of Education exploring the federative question in order to reveal the complete compatibility of the organization of the National System of Education with the federative regime. Thereafter, it deals with the historical meaning of the National Plan of Education demonstrating that the plan is a demand of the system, since planned action is implicit in systematized education. Thus the National Plan of Education is fulfilling those goals and objectives for which it is responsible.

Evaluation of the performance of different plastics used to seal nylon cDNA arrays

cDNA arrays are a powerful tool for discovering gene expression patterns. Nylon arrays have the advantage that they can be re-used several times. A key issue in high throughput gene expression analysis is sensitivity. In the case of nylon arrays, signal detection can be affected by the plastic bags used to keep membranes humid. In this study, we evaluated the effect of five types of plastics on the radioactive transmittance, number of genes with a signal above the background, and data variability. A polyethylene plastic bag 69 μm thick had a strong shielding effect that blocked 68.7% of the radioactive signal. The shielding effect on transmittance decreased the number of detected genes and increased the data variability. Other plastics which were thinner gave better results. Although plastics made from polyvinylidene chloride, polyvinyl chloride (both 13 μm thick) and polyethylene (29 and 7 μm thick) showed different levels of transmittance, they all gave similarly good performances. Polyvinylidene chloride and polyethylene 29 mm thick were the plastics of choice because of their easy handling. For other types of plastics, it is advisable to run a simple check on their performance in order to obtain the maximum information from nylon cDNA arrays.

Gluttonous predators: how to estimate prey size when there are too many prey

Prey size is an important factor in food consumption. In studies of feeding ecology, prey items are usually measured individually using calipers or ocular micrometers. Among amphibians and reptiles, there are species that feed on large numbers of small prey items (e.g. ants, termites). This high intake makes it difficult to estimate prey size consumed by these animals. We addressed this problem by developing and evaluating a procedure for subsampling the stomach contents of such predators in order to estimate prey size. Specifically, we developed a protocol based on a bootstrap procedure to obtain a subsample with a precision error of at the most 5%, with a confidence level of at least 95%. This guideline should reduce the sampling effort and facilitate future studies on the feeding habits of amphibians and reptiles, and also provide a means of obtaining precise estimates of prey size.