1000 resultados para Jerarquía de seres
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Mode of access: Internet.
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Mode of access: Internet.
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Fil: Garimaldi, Horacio Rubén. Universidad Nacional de La Plata. Facultad de Humanidades y Ciencias de la Educación; Argentina.
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Fil: Garimaldi, Horacio Rubén. Universidad Nacional de La Plata. Facultad de Humanidades y Ciencias de la Educación; Argentina.
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Leishmania infantum is the main etiologic agent of visceral leishmaniasis in the New World. The pattern of distribution of leishmaniasis has changed substantially and has presented an emerging profile within the periphery of the Large Urban Centers. Leishmania infection can compromise skin, mucosa and viscera. Only 10% of the individuals infected develop the disease and 90% of human infection is asymptomatic. The main factors involved in the development of the disease are the host immune response, the vector’s species and the parasite’s genetic content. The sequencing of Leishmania isolated seeks to increase the understanding of the symptoms of individuals. The aim of this study was to evaluate the genetic diversity of circulating Leishmania strains among humans, and symptomatic and asymptomatic, and dogs from endemic areas of Rio Grande do Norte State and analyze sandflies from endemic areas for cutaneous and visceral disease. The genetic variability was evaluated by the use of markers hsp70 , ITS1 and a whole genome sequencing was also carried out. The amplified hsp70 and ITS1 of samples were analyzed and assembled using a Phred / Phrap package. The dendograms were constructed using the same methodology, but adding 500 bootstraps, followed by inferences on the relationships between Leishmania variants. The sequences of the 20 Brazilian isolates were mapped to the reference genome L. infantum JPCM5, using the Bowtie2 program and the identification of 36 contigs. The information of the valid SNPs were used in the PCA. SNPs were visualized by Geneious 7.1 and IGV. The genome annotations were transferred to their respective chromosomes and displayed on Geneious. The matching sequences of all chromosomes were aligned using Mauve. The phylogenetic trees were calculated according to maximum likelihood and JTT models. Sandflies were analyzed by PCR for the identification of Leishmania infection, a blood meal source and GAPDH sand fly. As a result, hsp70 and ITS1 were not capable of identifying genetic variability among human isolates from symptomatic and asymptomatic, and dogs. The complete sequencing of the 20 Brazilian isolates revealed a strong similarity between the circulating Leishmania strains in Rio Grande do Norte. The isolates collected in the city of Natal from humans and canines remained grouped in all analyzes, suggesting that there is genotypic and geographic proximity among the isolates. The isolated samples in the 1990s had a higher genotypic diversity when compared to freshly isolated samples. All isolates presented 36 chromosomes with variable ploidy among them, no correlation was found between the number of amastina genes copies, gp63, A2 and SSG with such clinic forms. In general, we did not find correlation between symptomatic and asymptomatic clinical forms and the gene content of the Brazilian isolates of Leishmania. 34,28% of the sandflies collected in the upper west region were L. longipalpis and the main sources of blood meal were humans, dogs and chickens.
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Leishmania infantum is the main etiologic agent of visceral leishmaniasis in the New World. The pattern of distribution of leishmaniasis has changed substantially and has presented an emerging profile within the periphery of the Large Urban Centers. Leishmania infection can compromise skin, mucosa and viscera. Only 10% of the individuals infected develop the disease and 90% of human infection is asymptomatic. The main factors involved in the development of the disease are the host immune response, the vector’s species and the parasite’s genetic content. The sequencing of Leishmania isolated seeks to increase the understanding of the symptoms of individuals. The aim of this study was to evaluate the genetic diversity of circulating Leishmania strains among humans, and symptomatic and asymptomatic, and dogs from endemic areas of Rio Grande do Norte State and analyze sandflies from endemic areas for cutaneous and visceral disease. The genetic variability was evaluated by the use of markers hsp70 , ITS1 and a whole genome sequencing was also carried out. The amplified hsp70 and ITS1 of samples were analyzed and assembled using a Phred / Phrap package. The dendograms were constructed using the same methodology, but adding 500 bootstraps, followed by inferences on the relationships between Leishmania variants. The sequences of the 20 Brazilian isolates were mapped to the reference genome L. infantum JPCM5, using the Bowtie2 program and the identification of 36 contigs. The information of the valid SNPs were used in the PCA. SNPs were visualized by Geneious 7.1 and IGV. The genome annotations were transferred to their respective chromosomes and displayed on Geneious. The matching sequences of all chromosomes were aligned using Mauve. The phylogenetic trees were calculated according to maximum likelihood and JTT models. Sandflies were analyzed by PCR for the identification of Leishmania infection, a blood meal source and GAPDH sand fly. As a result, hsp70 and ITS1 were not capable of identifying genetic variability among human isolates from symptomatic and asymptomatic, and dogs. The complete sequencing of the 20 Brazilian isolates revealed a strong similarity between the circulating Leishmania strains in Rio Grande do Norte. The isolates collected in the city of Natal from humans and canines remained grouped in all analyzes, suggesting that there is genotypic and geographic proximity among the isolates. The isolated samples in the 1990s had a higher genotypic diversity when compared to freshly isolated samples. All isolates presented 36 chromosomes with variable ploidy among them, no correlation was found between the number of amastina genes copies, gp63, A2 and SSG with such clinic forms. In general, we did not find correlation between symptomatic and asymptomatic clinical forms and the gene content of the Brazilian isolates of Leishmania. 34,28% of the sandflies collected in the upper west region were L. longipalpis and the main sources of blood meal were humans, dogs and chickens.
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Programa de doctorado: Patología Quirúrgica, Reproducción Humana, Factores Psicológicos y el Proceso de Enfermar. La fecha de publicación es la fecha de lectura
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p. 108
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La abundancia de figuras flotantes en la plástica de la Edad moderna nos invita a estudiar el movimiento que realizan ángeles y divinidades. Por su movimiento ingrávido, estos personajes se diferencian de aquellos que están sujetos a su centro de gravedad. Conocemos su movimiento a través de las representaciones de esta época, movimiento que ha sido inventado a partir de la experiencia sensorial y la imaginación. La expresión virtual del dinamismo en imágenes estáticas se apoya en recursos técnicos y formales que en el caso de la figura humana ingrávida están orientados a resaltar las características peculiares de su movimiento. La independencia de una superficie de apoyo transforma en ilimitado el espacio en el que se mueven los seres ingrávidos; y su independencia del centro de gravedad hace posible desplazamientos que son insólitos en la vida cotidiana. Por ello, todo recurso visual que sugiera esta condición contribuirá a expresar su libertad de trayectorias.
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Tesis de Administraci?n de Empresas
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A partir de la pregunta de investigación ¿Cuál es la política pública de planeación urbana de la ciudad de Medellín? el artículo presenta la denominada jerarquía normativa urbana especial como parte del referencial que ha dado y sigue dando lugar a la citada política pública. Para ello, inicialmente se reseña la figura de política pública dentro de la cual se definen las categorías de referencial de política y flujo decisional, propuestas en el modelo de análisis de Pierre Muller, desde las cuales se legitima la naturaleza pública de una política y se diferencia de la política de Estado o de gobierno. Seguidamente se abordan los componentes que conforman la jerarquía normativa urbana especial, comenzando por los desarrollados por la Corte Constitucional en su jurisprudencia; posteriormente los propuestos por la doctrina, frente a la cual se plantea la modificación de su esquema a partir de la derogación del artículo 7 de la Ley 388 de 1997, la actual composición de la función administrativa urbana desde la dispersión de competencias territoriales conferidas por ley, y el anacrónico rol de las áreas metropolitanas en materia urbana.
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Las majadas de ovinos funcionan socialmente dentro de un orden jerárquico que se encuentra formado por individuos dominantes (alfa) y subordinados (omega). El macho dominante es el encargado de la reproducción (Ungerfeld & Núñez, 2011). El animal más agresivo es el que ocupa la posición más alta y no está sujeto a las mismas presiones experimentadas por el resto de los miembros. La sexualidad y el comportamiento agresivo cambian en relación al ciclo reproductivo y al fotoperiodo. Así, los animales menos afectados por el fotoperiodo permanecen más agresivos y con mayor incremento en la libido, mientras que la actividad sexual de los animales de baja jerarquía es generalmente suprimida en presencia de los dominantes (Aguirre, 2004). Cuando se insemina artificialmente se busca calidad y congelabilidad seminal que será proporcionada por un buen carnero independientemente de su ranking social en el grupo. (Guerrero et al., 2009). El objetivo de este trabajo fue evaluar el efecto de la jerarquía social sobre la calidad seminal y la congelabilidad del eyaculado de carneros alfa y omega.