Dielectrophoretic approaches to sample preparation and analysis

Dielectrophoresis (DEP) has been used to manipulate cells in low-conductivity suspending media using AC electrical fields generated on micro-fabricated electrode arrays. This has created the possibility of performing automatically on a micro-scale more sophisticated cell processing than that currently requiring substantial laboratory equipment, reagent volumes, time, and human intervention. In this research the manipulation of aqueous droplets in an immiscible, low-permittivity suspending medium is described to complement previous work on dielectrophoretic cell manipulation. Such droplets can be used as carriers not only for air- and water-borne samples, contaminants, chemical reagents, viral and gene products, and cells, but also the reagents to process and characterize these samples. A long-term goal of this area of research is to perform chemical and biological assays on automated, micro-scaled devices at or near the point-of-care, which will increase the availability of modern medicine to people who do not have ready access to large medical institutions and decrease the cost and delays associated with that lack of access. In this research I present proofs-of-concept for droplet manipulation and droplet-based biochemical analysis using dielectrophoresis as the motive force. Proofs-of-concept developed for the first time in this research include: (1) showing droplet movement on a two-dimensional array of electrodes, (2) achieving controlled dielectric droplet injection, (3) fusing and reacting droplets, and (4) demonstrating a protein fluorescence assay using micro-droplets. ^

Voltage control on an uninhabited autonomous vehicle electrical distribution system

More-electric vehicle technology is becoming prevalent in a number of transportation systems because of its ability to improve efficiency and reduce costs. This paper examines the specific case of an Uninhabited Autonomous Vehicle (UAV), and the system topology and control elements required to achieve adequate dc distribution voltage bus regulation. Voltage control methods are investigated and a droop control scheme is implemented on the system. Simulation results are also presented.

Mobile case-based decision support for intelligent patient knowledge management

Hospitals everywhere are integrating health data using electronic health record (EHR) systems, and disparate and multimedia patient data can be input by different caregivers at different locations as encapsulated patient profiles. Healthcare institutions are also using the flexibility and speed of wireless computing to improve quality and reduce costs. We are developing a mobile application that allows doctors to efficiently record and access complete and accurate real-time patient information. The system integrates medical imagery with textual patient profiles as well as expert interactions by healthcare personnel using knowledge management and case-based reasoning techniques. The application can assist other caregivers in searching large repositories of previous patient cases. Patients' symptoms can be input to a portable device and the application can quickly retrieve similar profiles which can be used to support effective diagnoses and prognoses by comparing symptoms, treatments, diagnosis, test results and other patient information. © 2007 Sage Publications.

The effects of trans-generational exposure to ocean warming and acidification on life history and physiological traits in a marine polychaete

Human-assisted, trans-generational exposure to ocean warming and acidification has been proposed as a conservation and/or restoration tool to produce resilient offspring. To improve our understanding of the need for and the efficacy of this approach, we characterised life history and physiological responses in offspring of the marine polychaete Ophryotrocha labronica exposed to predicted ocean warming (OW: + 3 °C), ocean acidification (OA: pH -0.5) and their combination (OWA: + 3 °C, pH -0.5), following the exposure of their parents to either control conditions (within-generational exposure) or the same conditions (trans-generational exposure). Trans-generational exposure to OW fully alleviated the negative effects of within-generational exposure to OW on fecundity and egg volume and was accompanied by increased metabolic activity. While within-generational exposure to OA reduced juvenile growth rates and egg volume, trans-generational exposure alleviated the former but could not restore the latter. Surprisingly, exposure to OWA had no negative impacts within- or trans-generationally. Our results highlight the potential for trans-generational laboratory experiments in producing offspring that are resilient to OW and OA. However, trans-generational exposure does not always appear to improve traits, and therefore may not be a universally useful tool for all species in the face of global change.

Smoking during pregnancy by duration of residence among immigrants in Sweden 1991-2012 : A study on health inequalities

This study revisits the effect of duration by residence in relation to smoking during pregnancy. It contributes to the literature by incorporating a health inequity perspective, and discusses whether immigrants tend to converge with Swedish women’s smoking. The study is based on Swedish Medical Birth Register and includes 1 1864 52 pregnancies between 1991 and 2012. Logistic regression was performed to attain crude and adjusted Odds Ratios and 95 % confidence intervals. Immigrants’ are divided by categorizing countries of origin depending on levels of Human Development (IHDI). Overall immigrant women show low levels of smoking during pregnancy when they arrive to Sweden, by duration of residence levels of smoking increase and converge with smoking patterns of Swedish women. I found that there are differences in smoking patterns depending on IHDI of the country. Immigrant women of higher categories of IHDI show higher levels of smoking although the increase of smoking is higher among immigrant women from categories of lower IHDI. However, immigrant women’s smoking during pregnancy is affected by duration of residence, and the increased smoking is associated with health inequalities related to their country of origins IHDI, and by socioeconomic inequalities in Sweden. 

Suspecting Renal Artery Thrombosis

Renal artery thrombosis is a threatening clinical diagnosis, in which renal infarction may occur. Often misdiagnosed, for mimicking other common diseases, it should be considered in persistent flank pain to improve care and reduce morbidity. We review a case of a healthy, 57 year-old woman with renal artery thrombosis mimicking pyelonephritis and renal calculus obstruction, highlighting features of this clinical condition. An accurate diagnosis is essential for optimal management and prompts treatment, which still remains to be defined.

A comparison of the physiological demands of rugby union match-play when determined by absolute (ABS) or individual (IND) velocity bands in Global Positioning System (GPS) analysis

Since turning professional in 1995 there have been considerable advances in the research on the demands of rugby union, largely using Global Positioning System (GPS) analysis over the last 10 years. A systematic review on the use of GPS, particularly the setting of absolute (ABS) and individual (IND) velocity bands in field based, intermittent, high-intensity (HI) team sports was undertaken. From 3669 records identified, 38 studies were included for qualitative analysis. Little agreement on the definition of movement intensities within team sports was found, only three papers, all on rugby union, had used IND bands, with only one comparing ABS and IND methods. Thus, the aim of this study was to determine if there is a difference in the demands within positions when comparing ABS and IND methods for GPS analysis and if these differences are significantly different between the forward and back positional groups. A total of 214 data files were recorded from 26 players in 17 matches of the 2015/2016 Scottish BT Premiership. ABS velocity zones 1-7 were set at 1) 0-6, 2) 6.1-11, 3) 11.1-15, 4) 15.1-18, 5) 18.1-21, 6) 21.1-15 and 7) 25.1-40km.h-1 while IND zones 1-7 were 1) <20, 2) 20-40, 3) 40-50, 4) 50-70, 5) 70-80, 6) 80-95 and 7) 95-100% of player’s individually determined maximum velocity (Vmax). A 40m sprint test measured Vmax using OptaPro S4 10 Hz (catapult, Australia) GPS units to derive IND bands. The same GPS units were worn during matches. GPS outputs analysed were % distance, % time, high intensity efforts (HIEs) over 18.1 km.h-1 / 70% max velocity and repeated high intensity efforts (RHIEs) which consists of three HIEs in 21secs. General linear model (GLM) analysis identified a significant difference in the measurement of % total distance covered, between the ABS and IND methods in all zones for forwards (p<0.05) and backs (p<0.05). This difference was also significant between forwards and backs in zones 1, shown as mean difference ± standard deviation (3.7±0.7%), 6 (1.2±0.4%) and 7 (1.0±0.0%) respectively (p<0.05). Percentage time estimations were significantly different between ABS and IND analysis within forwards in zones 1 (1.7±1.7%), 2 (-2.9±1.3%), 3 (1.9±0.8%), 4 (-1.4±0.8%) and 5 (0.2±0.4%), and within backs in zones 1 (-10±1.5%), 2 (-1.2±1.1%), 3 (1.8±0.9%) and 5 (0.6±0.5%) (p<0.05). The difference between groups was significant in zones 1, 2, 4 and 5 (p<0.05). The number of HIEs was significantly different between forwards and backs in zones 6 (6±2) and 7 (3±2). RHIEs were significantly different between ABS and IND for forwards (1±2, p<0.05) although not between groups. Until more research on the differences in ABS and IND methods is carried out, then neither can be deemed a criterion method. In conclusion, there are significant differences between the ABS and IND methods of GPS analysis of the physical demands of rugby union, which must be considered when used to inform training load and recovery to improve performance and reduce injuries.

Interoperability in pedagogical e-learning services

The ultimate goal of this research plan is to improve the learning experience of students through the combination of pedagogical eLearning services. Service oriented architectures are already being used in eLearning but in this work the focus is on services of pedagogical value, rather then on generic services adapted from other business systems. This approach to the architecture of eLearning platforms raises challenges addressed by this work, namely: conceptual modeling of the pedagogical eLearning services domain; interoperability and coordination of pedagogical eLearning service; conversion of existing eLearning systems to pedagogical services; adaptation of eLearning services to individual learners. An improved eLearning platform will incorporate learning tools adequate to the domains it covers and will focus on the individual learner that uses it. With this approach we expect to raise the pedagogical value of eLearning platforms.

Review of the health inequalities infant mortality PSA target

This review was established to consider why, despite a general improvement in infant mortality rates, health inequalities in infant mortality between different social groups remain. It identifies a range of issues and makes recommendations relevant to the health inequalities 2010 PSA target. Its recommendations are being shared with the NHS and others before the department issues further guidance.

Recerca de l'eficiència en aplicacions SIG per Android

A causa de l'increment del nombre de consumidors de dades geogràfiques, sobretot de dispositius mòbils, els sistemes d'informació geogràfica es troben davant el repte de reduir el coll d'ampolla que suposa l'arquitectura client-servidor clàssica. Una de les solucions a aquest problema és la translació de part de la intel·ligència al node client, així com la creació de xarxes descentralitzades (peer to peer),L'objectiu d'aquesta recerca és demostrar la viabilitat d'una infraestructura client mòbil-servidor, on el client no és únicament un consumidor més, si no que es torna un node intel·ligent.Per fer aquest estudi s'ha desenvolupat una aplicació pel sistema operatiu Android que consumeix dades d'OpenStreetMap. Aquesta aplicació utilitza tècniques de tessel·lat, catching i descàrrega de dades en background segons la posició de l'usuari, per facilitar el consum i reduir el flux de dades intercanviades entre el client i el servidor. També s'ha creat un servei web intern al dispositiu mòbil per a la creació de xarxes peer to peer, les quals permetin un intercanvi de dades entre els terminals mòbils. En aquest treball s'ha demostrat la viabilitat de la infraestructura a través del impacte de l'ús de les tècniques comentades anteriorment sobre el dispositiu client. Per mesurar l'impacte s'ha tingut en compte la càrrega de la CPU (la qual repercuteix al consum de la bateria) i el temps de resposta del sistema. Els resultats de les proves realitzades indiquen que aquestes tècniques redueixen el temps de cerca de punts d'interès d'una manera dràstica, però que també tenen un alt impacte a la CPU i en el temps de càrrega, sobretot en dispositius amb menys capacitats.

Oliotietokannan muuttaminen relaatiotietokannaksi

Diplomityön tavoitteena oli parantaa oliotietokantaan perustuvan mökkivarausjärjestelmän pitkiä vasteaikoja ja epävakaata toimintaa sekä luoda tietokantaperusta uusille toiminnoille. Ratkaisua etsittiin korvaamalla oliotietokanta relaatiotietokannalla. Diplomityö on osa koko varausjärjestelmän uusintaa. Työn teoreettinen osuus käsittelee sekä olio- että relaatiotietokannan rakennetta ja oliomallin muuttamista relaatiomalliksi. Käytännön osassa esitellään relaatiotietokannan luominen vaiheittain ja kerrotaan tietojen siirron periaatteesta. Relaatiotietokannan havaittiin olevan vakaampi ja tehokkaampi vasteajoiltaan. Samoin sen vaatima levytilan ja muistin määrä oli pienempi kuin oliotietokannalla. Lisäksi siihen todettiin uusien järjestelmien liittämisen olevan yksinkertaisempaa.

Aihiovalmistustoiminnan kehittäminen ohutlevytuotetehtaassa

Työ käsittelee aihiovalmistustoiminnan kehittämistä ohutlevytuotetehtaassa. Tutkimuksen tavoitteena oli kehittää aihiovalmistustiimin toiminnan edellytyksiä uudistuvan laitekannan tuomien mahdollisuuksien avulla muuttuvassa johtamiskulttuurissa. Kirjallisuusosassa tarkastellaan konepajatoiminnan tunnuslukuja ja ohutlevytekniikoiden teoriaa. Konepajatekniikan ohella käsitellään mm. johtamismenetelmien kehitystä yleisesti sekä erityisesti prosessijohtamista. Kokeellisessa osassa tarkastellaan eri raaka-ainevaihtoehtojen soveltuvuutta ohutlevytuotteille ja –rakenteille sekä materiaalikäytön kehittämisen kustannusvaikutuksia. Laiteinvestoinnin vaikutuksia tarkastellaan käytössä olevan konekapasiteetin hyödyntämisen kannalta. Aihiovalmistustoiminnan kehittämistä tarkastellaan kahden vuoden aikajänteellä, jona aikana kohdeyrityksessä ollaan implementoimassa prosessijohtamiseen perustuvaa prosessiorganisaatiomallia. Tutkimustuloksia tullaan hyödyntämään kohdeyrityksen operatiivisessa työskentelyssä.

Hankinnan kehittäminen puunjalostusteollisuuden yrityksessä

Tämän työn tavoitteena on kehittää kohdeyrityksen hankintaa ja erityisesti poikkiorganisatorisesti toimivan hankintaelimen toimintaa. Hankinnan kehittäminen paketoidaan tässä työssä kehittämisohjelmaksi, joka muodostuu havaittujen heikkouksien ja merkittävimpien puutteiden pohjalta. Tässä työssä empiriaosa pohjautuu teoriaan, joka koostuu pääasiassa hankinnan organisoinnin ja hankintaprosessin käsittelystä. Hankinnan organisoinnin avulla luodaan pohjaa tämän työn lähtökohdalle eli edellä mainitun hankintaelimen perustamiselle ja hankintaprosessin käsittelyn avulla luodaan pohjaa kehittämisohjelmalle. Empiriaosan taustalla vaikuttaa vahvasti myös kohdeyrityksessä tehdyt haastattelut sekä osallistuvan havainnoinnin kautta saadut tiedot. Hankinnasta saatavien hyötyjen mittaaminen on hyvin hankalaa, myös tästä työstä saatavia hyötyjä voidaan tuloksellisesti todeta vasta kun kehittämisohjelmaa on hyödynnetty tietty ajanjakso. Saatavia hyötyjä ei kuitenkaan voida tällöinkään mitata, mikäli hankinnalla ei ole käytettävissä sille suunniteltuja mittareita.

Key factors in U.S. Government support of business internationalization in Finland Case: U.S. Embassy in Helsinki

This study discusses the importance of government intervention for companies and the expansion of national innovation systems. The purpose of the study is to examine the impact which the U.S. Embassy in Helsinki can have on Finnish businesses through their business support programs and events. The embassy has shifted focus in recent years with the creation of an innovation center and increased business services. The study has sub-objectives to discover the critical factors for producing impact, host and home based factor interaction, and effects produced by these initiatives. The theoretical background of the study consists of literature relating to the concepts of national innovation systems and government intervention. The empirical research conduct for this study is based on interviews with experts from the environment surrounding the U.S. Embassy in Helsinki, Finland and participation in embassy events. The data was collected between March 2014 and September 2015. Seven interviews were conducted; five with representatives of the U.S. Embassy and two with related organizations. Thematic analysis was used to categorize and interpret interview and observation data. The use of an impact radar was implemented as a basis for analysis. This study finds that the internationalization of national innovation systems provides interesting opportunities and challenges for national governments. The opportunity to provide services to foreign companies by an embassy in a stable environment opens the possibility to create positive notice and relations with the host country. The increased connections and inputs to the national innovation system of the home country have the potential to increase knowledge absorption and create positive growth. The most effective way for governments to encourage businesses is to create incentives and reduce barriers. The services are best aimed at small to medium sized companies in the early stages of development. The findings of this report suggest that the most critical factors for producing impact on companies are the ability to disseminate information effectively, the ability to create a positive image of the country, the ability to foster effective networks between the two countries, and the ability to facilitate the internationalization of companies. In the best cases, the embassy is able to create incentives to internationalize to the United States and reduce barriers which are encountered by companies. Future research is necessary to fully understand the impact of business services provided by an embassy can have on the political and economic relations of countries, and on particular industry sectors. The institutional setting provided by the embassy’s focus on business relations provides a rich environment for further study in a number of areas.