自动导引车多Agent系统研究

多AGV系统的研究是移动机器人研究的一个重要方向，随着工厂自动化的不断发展及实际需求的推动，对多AGV系统的研究日益广泛和深入。多Agent理论是本文的核心指导理论，它来源于分布式人工智能（DAI）。分布式人工智能分为分布式问题求解（DPS）和多Agent系统（MAS）两个方向。由于MAS比DPS更能体现人类社会智能，具有更大的灵活性和适应性，更适合开放、动态的世界环境，因而越来越受到人们的重视。本文对Agent理论、Agent的基本结构、Agent的应用思想、多Agent系统的协调与协作等进行了详细地介绍。在总结了大量前人研究成果的基础上，本文认为有序是社会性的重要内涵，自主和有序是一个问题的两个方面，不能偏执一端，自主性体现了系统的灵活性，有序则体现了系统的效率。因此，我们提出了集中与分布相结合的控制机制，并成功地应用于多AGV系统的运行控制。其中涉及到了任务调度、路径规划、车辆的最优数量、通信机制、与生产系统的集成等AGES研究中的基本问题。

基于Multi-Agent的生产过程建模与仿真技术研究

某些流程行业由于采用按配方进行分组加工的模式组织生产,在排产时存在多条路径调度优化的问题,应用一般的优化算法对于现场在线调度难以给出满意结果,而基于Agent的过程仿真在解决离散、非线性系统模拟方面有显著的优势,本文采用Agent的方法对生产过程建模,然后对方案组内的备选方案进行仿真,通过对比各方案的仿真结果找到最优的方案作为执行方案,为现场的优化排产提供决策支持。

基于多Agent制造过程的建模方法

分析了制造系统与制造过程之间的关系;论证了从过程的角度对制造进行建模更恰当;结合Agent和π演算的特点,给出Agent制造系统描述模型及基于π演算的单个Agent的BDI模型,并指出Agent和π演算结合的制造过程模型有利于进行优化目标在不同制造过程层次的分解,不论从方法的角度还是实现的角度,都适合复杂系统建模。Agent和π演算相结合可以有效分析并解决离散事件的建模与仿真中的问题。

Drug-protein interaction studied by technique of microdialysis combined with high performance liquid chromatography

Drug-protein binding is an important process in determining the activity and fate of a pharmaceutical agent once it has entered the body. This review examines the method of microdialysis combined with high-performance liquid chromatography (HPLC) that has been developed;by ours to study such interactions, in which the microdialysis was applied to sample the free drug in the mixed solution of drug with protein, and HPLC to quantify the concentration of free drug in the microdialysate. This technique has successfully been used for determining various types of binding interactions between the low affinity drugs, high affinity drugs and enantiomers to HSA. For the case of competitive binding of two drugs to a protein in solution, a displacement equation has been derived and examined with four nonsteroidal anti-inflammatory drugs and HSA as model drugs and protein, respectively. Microdialysis with HPLC was adopted to determine simultaneously the free solute and displacing agent in drug-protein solutions. The method is able to locate the binding site and determine affinity constants even up to 10(7) L/mol accurately.

Determination of total iron binding capacity of serum by capillary electrophoresis

A capillary electrophoresis (CE) technique for determining total iron binding capacity (TIBC) of serum has been developed. The optimum serum pretreatment involves the following major steps: at first, saturate serum transferrin with Fe+3; then, dissociate them completely after removing excess unbound Fe. Finally, complex the released iron with phenanthroline, a chromophore, to make suitable for the CE analysis. Ammonium acetate (pH = 5.0) was used as CE background electrolyte solution. In this system, a good linear correlation coefficient was maintained over the range 0.5 similar to 10 mu M (r = 0.9979, n =12). Seven adult serum samples were studied and the TIBC parameters measured. In the present system, 10 similar to 30 mu L serum is sufficient for determination. The study shows that the CE technique described is a powerful method for rapid, efficient, sensitive and reliable analysis and hence particularly suitable for clinical application.

Capillary electrophoresis study of human serum albumin binding to basic drugs

The applicability of capillary electrophoresis/frontal analysis (CE/FA) for determining the binding constants of the drugs propranolol (PRO) and verapamil (VER) to human serum albumin (HSA) was investigated. After direct hydrodynamic injection of a drug-HAS mixture solution into a coated capillary (32 cm x 50 mu m i.d.), the basic drug was eluted as a zonal peak with a plateau region under condition of phosphate buffer (pH 7.4; ionic strength 0.17) at 12 kV positive running voltage. The unbound drug concentrations measured from the plateau peak heights had good correlation coefficients, r > 0.999. Employing the Scatchard plot, the Klotz plot and nonlinear regression, the drug protein binding parameters, the binding constant and the number of binding sites on one protein molecule, were obtained. The binding constant obtained was compared to a reported equilibrium dialysis result and they are basically in good agreement.