Elaboração e aplicação de um Programa de Treino de Técnica de Pontas para os alunos do 3º ano de Dança, da Academia de Dança do Vale do Sousa, com vista à preparação de Variações de Repertório Clássico

Relatório Final de Estágio apresentado à Escola Superior de Dança, com vista à obtenção do grau de Mestre em Ensino de Dança.

Sprachförderpraxis unter den Bedingungen der Diglossie. Zur "Sprachentrennung" bzw. "Sprachenmischung" als Normalität im Kindergartenalltag der deutschsprachigen Schweiz

Im vorliegenden Beitrag wird auf die pädagogische Qualität sprachlicher Förderpraxis im Feld des Kindergartens unter den curricularen Vorgaben und den vorgegebenen Diglossie-Bedingungen in der deutschsprachigen Schweiz deskriptiv-analytisch eingegangen. Durch unsere ethnographisch angelegte Feldstudie konnten wir aufzeigen, dass die sprachliche Förderung in ihrer praktischen Realisierung sowohl Sprachenmischung als auch Sprachentrennung voraussetzt und zugleich hervorbringt. Wir diskutieren unsere Ergebnisse auf der Basis von neueren soziolinguistisch fundierten pädagogisch-didaktischen Ansätzen, die auf das gesamte linguistische Repertoire mehrsprachiger Individuen (Lehrpersonen und Kinder) fokussieren und somit zur Qualitätsentwicklung des (vor-)schulischen Sprachunterrichts in der deutschsprachigen Schweiz beitragen könnten. (DIPF/Orig.)

Les plasmocytes et leur niche: Étude de la génération de plasmocytes humains in vitro

Chez l’humain, les lymphocytes B mémoires IgG+ et IgA+ sont des cellules clés de l’immunité humorale. Ces cellules mémoires sont maintenues à long-terme dans notre organisme. Elles représentent une défense rapide et efficace contre toutes les infections que nous avons déjà vaincues pendant notre vie. Ces cellules mémoires qui rencontrent à nouveau leur antigène se différencient rapidement en plasmocytes à courte vie, et permettent la sécrétion massive d’immunoglobuline (Ig). La contrepartie mémoire de ces cellules sont les plasmocytes à longue vie qui sont présents dans les niches de la moelle osseuse et y sécrètent en permanence des anticorps protecteurs qui circulent dans le sang. Ces cellules sécrétrices peuvent avoir une durée de vie allant de dizaines d’années à la vie entière de l’individu. Les patients qui reçoivent des traitements de chimiothérapie ou de radiothérapie sont privés de ces cellules mémoires détruites par ces traitements au même titre que les cellules cancéreuses. Ces patients deviennent vulnérables aux infections et leur survie dépend de la régénération rapide de leur système hématopoïétique. Notre équipe a déjà mis au point une méthode pour préparer de grandes quantités des cellules mémoires capables de sécréter des IgG et des IgA. Les présents travaux visent à générer des plasmocytes fonctionnels et capables de survivre à long terme in vitro. La stratégie expérimentale visait à établir des conditions permettant de se rapprocher de l’environnement de la moelle osseuse. Dans un premier temps, nous avons étudié les paramètres permettant la différenciation des lymphocytes B mémoires en plasmocytes. Étant donné l’importance du potentiel redox dans l’environnement de la moelle osseuse, nous avons d’abord tenté d’en contrôler l’impact avec un antioxydant, le N-acétyle cystéine (NAC). Nos résultats ont démontré que le NAC avait un effet significatif et diminuait la phosphorylation de la protéine STAT3 en raison d’une inhibition des kinases JAK2 et JAK3. Étonnamment, cet antioxydant retardait la différenciation de nos lymphocytes B qui étaient stimulés avec une forte interaction CD40-CD154. Par la suite, la comparaison des interactions CD40-CD154 et CD27-CD70 a permis de conclure qu’il était essentiel de réduire à son minimum l’interaction CD40-CD154 et qu’il fallait ajouter les cytokines IL-6 et IL-10. Les cellules CD31+CD38+CD138+ générées présentaient un phénotype similaire à celui des plasmocytes de la moelle osseuse. Malheureusement la fréquence de ces cellules était faible et leur viabilité insuffisante. Afin d’augmenter la survie de ces cellules le dernier volet de nos travaux visait à se rapprocher des niches de la moelle osseuse. Notre but a été atteint en ajoutant des cellules mésenchymateuses issues de la moelle osseuse en présence de 8% de dioxygène (O2). Les cellules CD31+CD38+CD138+ générées ont une excellente viabilité et représentent plus de 50% des cellules totales en culture. De plus, le modèle de culture est maintenant établi dans un milieu exempt de sérum et de protéines animales. Dans l’ensemble, nos résultats permettent de proposer la production ex vivo de plasmocytes autologues avec une perspective thérapeutique pour réduire les risques d’infections des patients devenues immunodéficients, suite à un traitement de radiothérapie ou de chimiothérapie.

Introdução à música contemporânea portuguesa de viola d’arco : um estudo de caso

Esta dissertação baseia-se no estudo da introdução da música contemporânea portuguesa de viola d’arco num grupo de alunos de viola d’arco da Casa Pia de Lisboa, através de composições encomendadas a nove compositores portugueses. Incide também na análise das atitudes em relação à música contemporânea portuguesa por parte de professores de viola d’arco portugueses. Procurou-se ampliar o repertório da viola d’arco através da encomenda de obras didáticas, e o repertório expressivo dos alunos envolvidos. Verificou-se que a introdução tardia da música contemporânea no ensino da música e a desatualização dos programas podem condicionar a atitude dos músicos em relação à mesma, sendo importante uma mudança de paradigma. Parecem não existir dificuldades técnicas na abordagem a estas obras por parte dos alunos. Houve também uma aceitação por parte dos professores em introduzir estes conceitos, tendo existido igualmente uma abertura por parte dos compositores contemporâneos envolvidos, em criar obras didáticas para viola d’arco.

Evaluation of vegetation recovery after fire: a case study in Calde, central region of Portugal

Remote Sensing has been used for decades, and more and more applications are added to its repertoire. With this study we aim to show the use of Remote Sensing in the field of vegetation recovery monitoring in burned areas and the added value of data with a high spatial resolution. This was done by analysing both Landsat 7 and 8 scenes, after the forest fire of summer 2012 in the parish of Calde, in the central region of Portugal, as well as an orthophoto produced with images acquired by an unmanned aerial vehicle.

Arquitetura residencial em João Pessoa-PB a experiência moderna nos anos 1970

In the mid-1980s, the magazine Projeto published the Actual Brazilian Architecture catalogue presenting texts by Hugo Segawa and Ruth Verde Zein with a corpus of works and engaged architects of the 1960s and 1970s. To comprehend the Brazilian architectural production post-1964, in those years of the 1980s, became a significant mission to reactivate the Brazilian architectural debate weakened by the military dictatorship. In his doctoral thesis Spadoni (2003) deals with the different ways which characterizes the Brazilian architectural production of the 1970s. Marked by inventiveness, this production was in tune with the modern thinking and in the transition period between the 1970s and the 1980s it synchronized with the international debate about post-modern architecture. Considering Spadoni s doctoral thesis, this work deals with the modern experience observed in the one-family-houses built in the seventies in João Pessoa. Some modern experiences were not clear outside, to observe it, it was necessary to search for the type of experience into the spatial disposition and of the know-how constructive, because into the appearance some houses not make explicit the use of the modern language. Other observed experiences allude to the repertoire of the Brazilian period in the years 1940s-1960s, to the experience of the modern architecture in São Paulo of the 1960s, to the experiences in which the climate of the Northeastern region strongly influenced the architectural conception. We can also find in a reduced number of houses a particular experience: it refers to experiences that expose the constructive doing, which leave the material apparent and apply to the residential type the experience of the industrial pre-fabricated buildings

Casas modernas na orla marítima de João Pessoa 1960 a 1974

This paper studies modern houses built in the neighborhoods of Cabo Branco, Tambaú and Manaíra by the seafront in Joao Pessoa, built between 1960 and 1974. We start from the already widespread notion that Brazilian Modern Architecture was inspired by foreign ways, mainly European but also American here recast, adapted, often innovating repertoire and ideas received (Y. BRUAND, 2005; H. SEGAWA, 2002; C. E. COMAS, 2002; C. E. COMAS, 2002; M. M. ACAYABA e S. FICHER, 1982; M. B. C. ARANHA, 2008; F. C. L. LARA, 2001; R. V. ZEIN, 2005; L. E. AMORIM, 1999, C. V. STINCO, 2010). With this look, after a field study, we collected 61 specimens in the collection of the Central Archive of João Pessoa City Hall, in order to identify which reformulations, adaptations or innovations would exist in modern houses built along the coast in João Pessoa. As we try to analyze the houses by what the bibliography had suggested (G. C. ARGAN, 1992; L. CORBUSIER, 2002; C.E.D. e M. ADRIÀ, 2007; K. FRAMPTON, 1997; H. H. HITCHCOCK, 1976; L. BENEVOLO, 2004; R. DE FUSCO, 1992, N . PEVSNER, 2002; M. RAGON, 1986; B. RISEBERO, 1982; E. ROBBINS, 1997; W. J. R. CURTIS, 1982; V. SCULLY, 2003; B. ZEVI, 1984; D. DUNST, 1999; A. COLQUHOUN, 2002; R. WESTON, 2005; A. IÑAKI, 2006; J. PETER, 1994) the starting idea seemed to us not sufficiently developed. So we decided, first, to undertake a literature review comparing speech and image of modern houses most often cited by international and national literature, following a script freely inspired the Vitruvian triad: the functional and spatial (sectorization, guidance, spatiality, movement); constructive aspects (structural elements, modulation, deck, sealing), and aesthetic aspects (composition, apertures, ornaments) (Cap.1), then cast a look under this same route in 61 specimens obtained initially, trying to verify any specificities they would have (Cap.2). Failing to deepen the analysis of all these examples, we chose 10 projects which were redesigned and described in more detail to which we supplement with the aspects of place - location / lot location, access, axiality. (E. C. MAHFUZ, 2002; J. C. MIGUEL, 2000; E. C. CHEREGATI, 2007; M. COTRIM, 2007). (Cap. 3). The documentation and description resulting allowed us to approach some related questions about the canonical transcripts hybridizing, adoptions and any vernacular innovations of modern houses along the coast, We conclude that the appellants and anachronistic elements isolates found in each of the studied bind to the Brazilian Modern Architecture

NK Cells of Kidney Transplant Recipients Display an Activated Phenotype that Is Influenced by Immunosuppression and Pathological Staging

To explore phenotype and function of NK cells in kidney transplant recipients, we investigated the peripheral NK cell repertoire, capacity to respond to various stimuli and impact of immunosuppressive drugs on NK cell activity in kidney transplant recipients. CD56(dim) NK cells of kidney transplanted patients displayed an activated phenotype characterized by significantly decreased surface expression of CD16 (p=0.0003), CD226 (p<0.0001), CD161 (p=0.0139) and simultaneously increased expression of activation markers like HLA-DR (p=0.0011) and CD25 (p=0.0015). Upon in vitro stimulation via Ca++-dependent signals, down-modulation of CD16 was associated with induction of interferon (IFN)-gamma expression. CD16 modulation and secretion of NFAT-dependent cytokines such as IFN-gamma, TNF-alpha, IL-10 and IL-31 were significantly suppressed by treatment of isolated NK cells with calcineurin inhibitors but not with mTOR inhibitors. In kidney transplant recipients, IFN-gamma production was retained in response to HLA class I-negative target cells and to non-specific stimuli, respectively. However, secretion of other cytokines like IL-13, IL-17, IL-22 and IL-31 was significantly reduced compared to healthy donors. In contrast to suppression of cytokine expression at the transcriptional level, cytotoxin release, i.e. perforin, granzyme A/B, was not affected by immunosuppression in vitro and in vivo in patients as well as in healthy donors. Thus, immunosuppressive treatment affects NK cell function at the level of NFAT-dependent gene expression whereby calcineurin inhibitors primarily impair cytokine secretion while mTOR inhibitors have only marginal effects. Taken together, NK cells may serve as indicators for immunosuppression and may facilitate a personalized adjustment of immunosuppressive medication in kidney transplant recipients.

THREE AMERICAN INTERNATIONAL PIANO COMPETITIONS: THE PIANO WORKS THEY COMMISSIONED BETWEEN 1962 AND 2012

Over a period of 50 years—between 1962 and 2012—three preeminent American piano competitions, the Van Cliburn International Piano Competition, the University of Maryland International Piano Competition/William Kapell International Piano Competition and the San Antonio International Piano Competition, commissioned for inclusion on their required performance lists 26 piano works, almost all by American composers. These compositions, works of sufficient artistic depth and technical sophistication to serve as rigorous benchmarks for competition finalists, constitute a unique segment of the contemporary American piano repertoire. Although a limited number of these pieces have found their way into the performance repertoire of concert artists, too many have not been performed since their premières in the final rounds of the competitions for which they were designed. Such should not be the case. Some of the composers in question are innovative titans of 20th-century American music—Samuel Barber, Aaron Copland, Leonard Bernstein, John Cage, John Corigliano, William Schuman, Joan Tower and Ned Rorem, to name just a few—and many of the pieces themselves, as historical touchstones, deserve careful examination. This study includes, in addition to an introductory overview of the three competitions, a survey of all 26 compositions and an analysis of their expressive characteristics, from the point of view of the performing pianist. Numerous musical examples support the analysis. Biographical information about the composers, along with descriptions of their overall musical styles, place these pieces in historical context. Analytical and technical comprehension of this distinctive and rarely performed corner of the modern classical piano world could be of inestimable value to professional pianists, piano pedagogues and music educators alike.