Caracterização dos genes scMUTM1 e scMUTM2 de cana-de-açúcar

Plants are organisms sessile and because of this they are susceptible to genotoxic effects due to environmental exposure such as light [including ultraviolet (UV)], heat, drought and chemicals agents. Therefore, there are differents pathways in order to detect a lesion and correct. These pathways are not well known in plants. The MutM/Fpg protein is a DNA glycosylase that is responsible for detect and correct oxidative lesions. In the sugarcane genome, it was found two possible cDNAs that had homology to this protein: scMUTM1 and scMUTM2. The aim of this work was to characterize the role of these cDNAs in plants. In order to do this, the expression level after oxidative stress was evaluated by semiquantitative RT-PCR. Another point analyzed in order to obtain the full-length gene, it was to use a sugarcane genomic library that was hybridized with both cDNAs as a probe. It was found two clones that will bought and sequenced. The promoter region was also cloned. It was obtained sequences only for scMUTM2 promoter region. The sequences obtained were divided into six groups. It was found regulatory motifs such as TATA-box, CAAT-box, oxidative stress element response and regulatory regions that response to light. The other point analyzed was to characterize the N-terminal region by PCR constructs. These constructs have deletions at 5 region. These sequences were introduce into Escherichia coli wild type strain (CC104) and double mutant (CC104mutMmutY). The results showed that proteins with deletions of scMUTM1 N-terminal region were able to complement the Fpg and MutY-glycosylase deficiency in CC104 mutMmutY reducing the spontaneous mutation frequency

Interação entre professora e alunos em salas de aula com proposta pedagógica de educação inclusiva

Correntes teóricas presentes na literatura científica têm demonstrado a importância das relações interpessoais para o processo de construção do conhecimento. É no contexto da interação professor e aluno que se configura a relação entre as necessidades educacionais dos alunos e as respostas pedagógicas a elas disponibilizadas, o que envolve o domínio do conhecimento pelo professor, sua capacitação técnico-científica, a competência de ensinar pesquisando, as características sócio-culturais e o perfil psicológico dos atores sociais envolvidos- professor e aluno. Buscando melhor compreender este universo, elaborou-se este estudo que teve como objetivo descrever as interações ocorridas entre uma professora, e seus alunos, em classes em que se propunha adotar uma prática pedagógica inclusiva. Os dados foram coletados em 2 salas de aula, em escola estadual de Ensino Fundamental, no município de Bauru. O processo de coleta de dados se deu através do registro da realidade de sala de aula em vídeo tape. Optou-se por este método, por permitir a recuperação posterior dos dados. A coleta se deu no transcorrer do 1º semestre do ano letivo de 2001. A análise fundamentou-se em sistema prévio de categorias, e tratou os dados quantitativa e qualitativamente. Os resultados demonstraram peculiaridades e diferenças nas interações da professora com os seus alunos, em função da presença ou ausência da deficiência. Indicaram, também, que a interação vem demonstrando avanços na prática educacional, no que diz respeito à atenção pedagógica, da professora, ao aluno com deficiência.

Trypanocidal action of eupomatenoid-5 is related to mitochondrion dysfunction and oxidative damage in Trypanosoma cruzi

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Percepção de fala dos deficientes auditivos usando aparelho de amplificação com algoritmo de redução de ruído

Frequentemente, os indivíduos com perda auditiva têm dificuldade de entender a fala no ambiente ruidoso. OBJETIVO: O objetivo deste estudo foi avaliar clinicamente o desempenho dos indivíduos adultos com deficiência auditiva neurossensorial, com relação à percepção da fala, utilizando o aparelho de amplificação sonora individual digital com o algoritmo de redução de ruído denominado Speech Sensitive Processing, ativado e desativado na presença de um ruído. MATERIAL E MÉTODO: Este estudo de casos foi realizado em 32 indivíduos com deficiência auditiva neurossensorial de graus leve, moderado ou leve a moderado. Foi realizada a avaliação por meio de um teste de percepção de fala, onde se pesquisou o reconhecimento de sentenças na presença de um ruído, para obter a relação sinal/ruído, utilizando o aparelho auditivo digital. RESULTADOS: O algoritmo pôde proporcionar benefício para a maioria dos indivíduos deficientes auditivos, na pesquisa da relação sinal/ruído e os resultados apontaram diferença estatisticamente significante na condição em que o algoritmo encontrava-se ativado, comparado quando o algoritmo não se encontrava ativado. CONCLUSÃO: O uso do algoritmo de redução de ruído deve ser pensado como alternativa clínica, pois observamos a eficácia desse sistema na redução do ruído, melhorando a percepção da fala.

Caracterização da caprinocultura nas microrregiões da chapada do Apodi e de Angicos do Estado do Rio Grande do Norte

The objectives of this study were to characterize the goat production systems and to identify the factors limiting the production chain and develop solutions to improve the goat industry in the Rio Grande do Norte State, Brazil. One hundred and sixty two questionnaires were applied to goat farmers looking for productive, economic and social information about the utilized systems in the micro regions of Chapada do Apodi and Angicos. The majority producers interviewed were male, aging from 31 to 50 years old, attended elementary school, associated to farm organizations and living with his family on farm. Their main concerns are feeding the heard and animal theft. Their average farmlands areas range from 11 to 50 hectares. Goat meat is the main production in Chapada do Apodi whereas milk is produced by farmers in Angicos micro region. The majority of goat production works with an extensive system, with low technological inputs. It was detected that the meat and milk production as the marketing of goat products is not well conducted. Other point to be improved is to increase the meat and dairy goat products to be available for consumers. Goat systems for meat and milk production has great to potential for this micro regions, although public policies for an organized supply chain is needed

A memória de curto prazo e a síndrome de down: a relação entre contextos de desenvolvimento

Down syndrome (DS) is one of the most frequent causes of intellectual disability, affecting one in every 600 to 1000 live births. Studies have demonstrated that people with DS have a lower capacity for short-term memory (STM) and working memory (WM), which affects their capability to learn new words and to follow spoken instructions, specially when they involve multiple information or consecutive orders/orientations. It seems that the basis of the learning process, as it happens with language and mathematics comprehension and reasoning, relies in the STM and WM systems. Individuals with DS are increasingly included in mainstream education, and yet, very few researches have been conducted to investigate the influence of memory development and the type of enrollment (regular school and special school). This study investigated the relationship between the type of school enrollment with the performance on STM tests and also, the relationship of this performance with early stimulation (ES). The tests used in the first research were the digit span, free recall, word recognition and subtests of the Wechsler Intelligence Scale for Children Third Edition (WISC-III). Individuals enrolled in the regular schools group had higher scores on the digit span test and the subtests of the WISC-III. In the free recall and recognition tests, no differences were found. This study indicates that the type of enrollment might influence the memory development of individuals with DS and clearly points the need for future investigations. In the second research, the tests used were the digit span, free word recall and subtests of the WISC-III. The test results showed better performance by adults that received ES before six months of age. The studies showed improvement in STM both in people who attended or were attending regular school, as well as those who benefited from ES before six months of age. However, some issues still need to be better understood. What is the relation between this stimulation with the individual s education? Since ES may reflect a greater family involvement with the individual, what is the role of emotional components derived from this involvement in the cognitive improvement? These and other questions are part of the continuity of this study

Violência doméstica contra adolescentes: o olhar dos educadores sociais

Domestic violence as a specific expression of violence against adolescents, is historically constructed from phenomenon of power relations that permeate the gender, ethnicity and social class, requiring differentiated attention. Sorting in A mass grave of what is understood by a child and teenager under the doctrines of the law of the minor and of irregular situation resulted, in General, in the preparation for the teenage audience, of policies and actions, fragile and inefficient with regard to domestic violence. Despite the status of children and adolescents clearly define what is meant by child and teenager, break with the menoristas doctrines and embrace the doctrine of integral protection, even if there is little guidance for the public actions and policies geared to the theme. Such deficiency contributes to characterize the current practice of social educators about domestic violence, marked by the absence of specific training; by inadequate working conditions; lack of preparation of the network of care. Thus, with the objective to start the discussion regarding the elements that make up the practice focused on this issue, the present study proposes to problematizing the design held by social educators working in public Social assistance of the city of Natal/RN, regarding the issue of domestic violence against adolescents, by paying attention to such things as their vocational integration, their training and their working conditions. Adopts norteadoras references as having its roots in the Pedagogia Problematizadora, Paulo Freire, and socio-historical perspective. The methodological procedure of nature quali-quantitative was lifting censitário and psychosocial characterization of educators together to State and local Departments of assistance; the application of questionnaire to educators, composed of open and closed issues; and observation and field journaling activities of their work. Of network professionals, 111 64 replied to the questionnaire, which represents more than 50% of the total. Psychosocial characterization exposes data as the predominance of low family income and female professionals; the note identified that do not have a professional technical parameter, and the activities carried out in accordance with the representations of each professional the respect of labour, problematic and adolescents involved, revealing an individual perspective of action. And a preliminary analysis of responses to the questionnaire pointed out that such professionals are subjected to precarious working conditions, as well as one realizes a relative ignorance about the network of care for adolescents at risk and ACE, all this resulting in restricted marginalizantes conceptions, and misleading information regarding domestic violence against teenagers.

Estratégias de aprendizagem utilizadas por estudantes cegos

Learning strategies can be understood as a planned sequence of procedures or activities, selected in order to facilitate the acquisition, storage and use of information. Although important to the learning process, the knowledge on these strategies is still insufficient, especially with regard to students with visual impairments. Therefore, this study aimed to characterize learning strategies used by blind and sighted students, registered in elementary education in schools and special institutions. Were participants 23 teachers, male and female, aged between 26 and 51 years, and 102 students, of whom 25 were blind and 77 seers, of both genders, registered on the 3rd to the 9th grade of elementary school, aged 7 to 16 years old. The instruments used were: field diary (students and teachers); structured questionnaire (teachers); sociodemographic questionnaire, interview and Assessment Scale of Learning Strategies for Elementary School (students). Initially were made observations in the classroom and the teachers received the questionnaires, with instructions for completion. Then were made the interviews with blind students and the scale was administered with these and with the seers. All instruments were administered individually. We conducted content analysis of the questionnaires with teachers and the interviews with blind students. The scale of strategies and sociodemographic questionnaire were analyzed with the help of descriptive and inferential statistics. It was noticed that the blind students use few learning strategies in the classroom, regardless of city, series, sex or age. It was found that teachers didn t receive training to deal with blind students, either during graduation or after have completed it, in such a way that few of them were able to inform about the learning strategies the students use, and demonstrated low ability to deal with these inefficiencies. It was also found that the blind and the sighted use cognitive and metacognitive strategies during learning, but those used by the blind seem to be more basic, low complexity, given that the seers have achieved higher scores on all subscales. We conclude that the repertoire of learning strategies for blind students is inflexible, requiring increments so that they can achieve significant results. It is important that teachers receive training to understand the learning strategies and how they positively influence learning

Manejo de irrigação por aspersão com base no Kc e adubação mineral na cultura de arroz de terras altas

A irrigação por aspersão diminui bastante o risco de perda da lavoura por deficiência hídrica e aumenta a produtividade de grãos, incentivando maior uso de tecnologias como adubação mineral. Com o objetivo de avaliar o efeito de diferentes manejos da água da irrigação por aspersão com base no coeficiente de cultura (Kc) e da adubação mineral sobre a cultura do arroz cv. IAC 201, foram instalados dois experimentos em Latossolo Vermelho Distrófico, em Selvíria (MS). O delineamento foi em blocos casualizados, com quatro repetições. Os tratamentos constituíram-se de precipitação pluvial natural e três manejos de água fornecidos por aspersão. O manejo (M2) foi realizado com base no Kc do arroz de terras altas. Os manejos M1 e M3 foram definidos como 0,5 e 1,5 vezes os Kcs utilizados em M2 respectivamente. em 1995/96, utilizou-se o esquema de parcelas subdivididas, sendo as subparcelas constituídas por dois níveis de adubação: AD1 - 12 kg de N, 90 kg de P2O5 e 30 kg de K2O ha-1, e AD2 - 24 kg de N, 180 kg de P2O5 e 60 kg de K2O ha-1. A deficiência hídrica da emergência da plântula até a diferenciação do primórdio da panícula provocou aumento do ciclo e redução do porte da planta. A deficiência hídrica entre os estádios de diferenciação do primórdio da panícula e os de emborrachamento reduziu o número de espiguetas por panícula. A utilização de 1,5 vezes os valores de Kc recomendados, no manejo da irrigação por aspersão proporcionou maior produtividade de grãos. Os níveis de adubação utilizados não influenciaram a resposta da cultura ao manejo da irrigação por aspersão.

Prevalência e fatores associados ao risco e a desnutrição em idosos institucionalizados

One of the most important problems in the elderly is a nutritional deficiency. Several physiological changes and the use of multiple drugs interfere with appetite, food intake and absorption of nutrients, which can lead to the risk and malnutrition in the elderly, especially among institutionalized. The present study aimed to evaluate the prevalence of malnutrition and risk for malnutrition and its associated factors in institutionalized elderly. The same can be characterized by the type individual, observational and cross-sectional. Obtaining the sample was through the records of individuals of long-stay institutions for the elderly in the city of Natal, RN. The elderly were evaluated through the Mini Nutritional Assessment (MNA) and triceps skinfold (TSF) and each senior or caregiver answered a questionnaire about information like type and dietary restrictions, accessibility to food, use of alcohol and tobacco, practice physical activity and appetite. Variables such as age, gender, education, marital status, time that the elderly living in the institution, the reason for the institutionalization and comorbidities were taken from the records of each senior. The frequency of food consumption of various food groups was assessed from the questionnaire frequency of feeding study Health, Wellbeing and Aging (HWA). Data were presented as means and standard deviations, absolute and relative frequencies. To analyze the frequency of consumption, there was a factor analysis with extraction of factors from the principal components analysis with varimax rotation. A bivariate analysis was performed using the chi-square and the magnitude of the effect observed by prevalence ratio (95% CI). The Poisson regression assessed the net effect of independent variables on the two outcomes, considering a significance level of 5%. We studied twelve Homes for the Aged totaling 381 seniors eligible for the study. The prevalence of risk of malnutrition was 46.1% (45.9 to 46.2) and malnutrition was 31.4% (31.2 to 31.5). The risk of malnutrition was significantly associated with the presence of urinary incontinence (RP = 1.444, 1.113 to 1.874) was associated with malnutrition and lack of appetite (RP = 1.757, 1.246 to 2.476), the fact that the individuals do not have access to food outside the institution (RP = 0.565, 0.337 to 0.946), low water consumption (RP = 1.646, 1.101 to 2.459) and dementia (PR = 1.537, 1.072 to 2.204). The high prevalence of malnutrition and risk of malnutrition in the study suggests that we should pay attention to information related to eating habits and the presence of comorbidities, as these can influence the nutritional status of this population

Produtividade e componentes da produção do amendoim da seca em razão da época de semeadura e da aplicação de cálcio

Este trabalho teve por objetivo estudar o efeito do calcário, como fornecedor de cálcio, em diferentes épocas de semeadura, na cultura do amendoim cultivar Tatu, sobre os componentes da produção e a produtividade de vagens em semeadura na época considerada seca. O experimento foi conduzido em um Latossolo Vermelho-Escuro, argiloso, em Selvíria, MS. Foram estudadas quatro épocas de semeadura do amendoim da seca (21/1, 4/2, 18/2 e 4/3), combinadas com quatro doses de Ca (0, 45, 90 e 135 kg ha-1 de Ca), aplicadas no sulco de semeadura, usando como fonte calcário dolomítico com PRNT de 90,1% e com teores de 21% de CaO e 18% de MgO. Foram avaliados o número de vagens por planta, o número de grãos por vagem e por planta, o peso de 100 grãos, o rendimento e a produtividade das vagens. Conclui-se que: 1) à medida que se atrasa a semeadura, há a probabilidade de ocorrer deficiência hídrica nos períodos vegetativo e reprodutivo, reduzindo a produtividade do amendoim da seca; 2) há boa produtividade de vagens quando a semeadura é realizada até o início do mês de fevereiro; 3) a resposta do amendoim da seca ao Ca é baixa em solos onde inicialmente se tem alto teor de Ca e saturação por bases considerada média, principalmente, quando a disponibilidade hídrica também é um fator limitante; 4) a aplicação de calcário no sulco de semeadura como meio de fornecimento de Ca para a cultura do amendoim é uma prática viável.

A assistência técnica e extensão rural no assentamento modelo: proposição e realidade

This discourse analyzes the technical assistance concerning the rural settlement, which is seen as a demand of the social movement that claims for agrarian reformation, and is a goal of II PNRA, launched in may 2004, as a essential national public politics on process of building and consolidation of settlement and familiar agriculture, proposing the return of technical assistance service and rural extension in Brazil, which were started in the and of 1940 s. We analyze, in particular, the technical assistance program, social and environmental agrarian reformation, on model settlement, a program created, especially, to the rural settlements, coordinated by INCRA. Based on the documental analyze and local study, it is noticed that the experience of technical assistance implemented on model settlement shows the non continuation and fragility on technical assistance actions to rural workers. This context goes against the lately technical assistance governmental apparatus, which ensures to make settlements stronger, to support and to get important the familiar agriculture system. This way, technical assistance execution, trough the tertiary service, follows the neoliberal strategy and, the State, besides decreases the estimate to public politics, takes its actions control from the State to the shared control through partnership and transferring of responsibility, expressing its historic lack of attention to the worker class demands. In spite of workers resistance, expectative and hope, the lack of these services, as well as the other politics deficiencies, which are necessary to the settlements, are endangering its activities and threatening its survival in the settlement

O Processo de revisão do benefício de prestação continuada-BPC: uma lógica de exclusão ou inclusão social

This research comprises a study about the social assessment performed by the Social Worker in the review process of the Benefit of Continued Installment. The Benefit of Continued Installment was implemented in 1996 and guarantees a minimum salary to the deficiency person and to an elder with sixty five years or more and that proves not to have ways to support neither himself/herself nor his/her own family. It is a demand to include in the BPC that the maximum income of a family does not exceed ¼ of minimum salary and that every two years this benefit to be revised to evaluate its continuity based in its original conditions. This study was carried out in the municipality of Natal/RN, with thirteen social workers, being the collection of data performed through interviews and social assessments of the users that count with the benefit. The results show that the users selected by the criterion of the income, present a profile of poverty and deprivations demonstrated through several situations survived in its daily life, indicative of vulnerability. It was demonstrated that the Social workers has relative autonomy in the evaluations along with the users and that it denotes the necessity of inclusion. However, by following the imposed criteria, it corroborates with the logic of exclusion. So, it is identified in the Municipality of Natal/RN, following the orientation given the politics of social work at national level, the implementation of revision of the BPC, for the social workers, from rigorous processes of selection and exclusions

O Benefício da Prestação Continuada BCP: as condições de vida dos usuários no Município de Macau/RN

This rescearch comprises a study of the life condictions of the users of the Continuous Installment Allowance in the city of Macau, RN. This benefit has been predicted by the Federal 1988 Constitution and regulated by the Social Assistance Organic Law (LOAS), but it has been carried out only since january 1996. It consists of an amount of half a minimum salary given to old-age and handicapped peoplewhose families mathch the per capita income criterion of less than 0,25 minimum salary. It is a least-income policy relud by the principle of the smallest elegibility. The study focused on aged or handicapped people who were receiving the BPC from 1996 to 2004. Through a quatitative analysis, the life reality of these users has been critically examined. In order to achieve this, it has been necessary to trace their social and economical profile. The research revealed the precarious life conditions of the aged and handicapeed people after they were given the BPC, as well as their interpretation of this politcal directive, and also the need for revision of the eligibility criteria so that a public policy of integral service to the basic needs of the social being can be accomplished

Uma estratégia dirigida a modelos e baseada em linguagem de descrição arquitetural para linhas de produtos de software

Model-oriented strategies have been used to facilitate products customization in the software products lines (SPL) context and to generate the source code of these derived products through variability management. Most of these strategies use an UML (Unified Modeling Language)-based model specification. Despite its wide application, the UML-based model specification has some limitations such as the fact that it is essentially graphic, presents deficiencies regarding the precise description of the system architecture semantic representation, and generates a large model, thus hampering the visualization and comprehension of the system elements. In contrast, architecture description languages (ADLs) provide graphic and textual support for the structural representation of architectural elements, their constraints and interactions. This thesis introduces ArchSPL-MDD, a model-driven strategy in which models are specified and configured by using the LightPL-ACME ADL. Such strategy is associated to a generic process with systematic activities that enable to automatically generate customized source code from the product model. ArchSPLMDD strategy integrates aspect-oriented software development (AOSD), modeldriven development (MDD) and SPL, thus enabling the explicit modeling as well as the modularization of variabilities and crosscutting concerns. The process is instantiated by the ArchSPL-MDD tool, which supports the specification of domain models (the focus of the development) in LightPL-ACME. The ArchSPL-MDD uses the Ginga Digital TV middleware as case study. In order to evaluate the efficiency, applicability, expressiveness, and complexity of the ArchSPL-MDD strategy, a controlled experiment was carried out in order to evaluate and compare the ArchSPL-MDD tool with the GingaForAll tool, which instantiates the process that is part of the GingaForAll UML-based strategy. Both tools were used for configuring the products of Ginga SPL and generating the product source code