Variability in the Stability and Productivity of Transfected Genes in Chinese Hamster Ovary (CHO) cells

In the field of biologics production, productivity and stability of the transfected gene of interest are two very important attributes that dictate if a production process is viable. To further understand and improve these two traits, we would need to further our understanding of the factors affecting them. These would include integration site of the gene, gene copy number, cell phenotypic variation and cell environment. As these factors play different parts in the development process, they lead to variable productivity and stability of the transfected gene between clones, the well-known phenomenon of “clonal variation”. A study of this phenomenon and how the various factors contribute to it will thus shed light on strategies to improve productivity and stability in the production cell line. Of the four factors, the site of gene integration appears to be one of the most important. Hence, it is proposed that work is done on studying how different integration sites affect the productivity and stability of transfected genes in the development process. For the study to be more industrially relevant, it is proposed that the Chinese Hamster Ovary dhfr-deficient cell line, CHO-DG44, is used as the model system.

Bayesian tools for zero counts in compositional data

The log-ratio methodology makes available powerful tools for analyzing compositional data. Nevertheless, the use of this methodology is only possible for those data sets without null values. Consequently, in those data sets where the zeros are present, a previous treatment becomes necessary. Last advances in the treatment of compositional zeros have been centered especially in the zeros of structural nature and in the rounded zeros. These tools do not contemplate the particular case of count compositional data sets with null values. In this work we deal with \count zeros" and we introduce a treatment based on a mixed Bayesian-multiplicative estimation. We use the Dirichlet probability distribution as a prior and we estimate the posterior probabilities. Then we apply a multiplicative modi¯cation for the non-zero values. We present a case study where this new methodology is applied. Key words: count data, multiplicative replacement, composition, log-ratio analysis

Enhancing learning beyond the classroom: blogging and other social networking tools

Blogging has become one of the key ingredients of the so-called socials networks. This phenomenon has indeed invaded the world of education. Connections between people, comments on each other posts, and assessment of innovation are usually interesting characteristics of blogs related to students and scholars. Blogs have become a kind of new form of authority, bringing about (divergent) discussions which lead to creation of knowledge. The use of blogs as an innovative, educational tool is not at all new. However, their use in universities is not very widespread yet. Blogging for personal affairs is rather commonplace, but blogging for professional affairs – teaching, research and service, is scarce, despite the availability of ready-to-use, free tools. Unfortunately, Information Society has not reached yet enough some universities: not only are (student) blogs scarcely used as an educational tool, but it is quite rare to find a blog written by University professors. The Institute of Computational Chemistry of the University of Girona and the Department of Chemistry of the Universitat Autònoma de Barcelona has joined forces to create “InnoCiència”, a new Group on Digital Science Communitation. This group, formed by ca. ten researchers, has promoted the use of blogs, twitters. wikis and other tools of Web 2.0 in activities in Catalonia concerning the dissemination of Science, like Science Week, Open Day or Researchers’ Night. Likewise, its members promote use of social networking tools in chemistry- and communication-related courses. This communication explains the outcome of social-network experiences with teaching undergraduate students and organizing research communication events. We provide live, hands-on examples and interactive ground to show how blogs and twitters can be used to enhance the yield of teaching and research. Impact of blogging and other social networking tools on the outcome of the learning process is very depending on the target audience and the environmental conditions. A few examples are provided and some proposals to use these techniques efficiently to help students are hinted

Neurocognition in schizophrenia : A study of the productivity and visibility of Spanish authors

Resumen tomado de la publicaci??n

Analysis and regeneration of hypermedia contents through Java and XML tools

This paper presents a tool for the analysis and regeneration of Web contents, implemented through XML and Java. At the moment, the Web content delivery from server to clients is carried out without taking into account clients' characteristics. Heterogeneous and diverse characteristics, such as user's preferences, different capacities of the client's devices, different types of access, state of the network and current load on the server, directly affect the behavior of Web services. On the other hand, the growing use of multimedia objects in the design of Web contents is made without taking into account this diversity and heterogeneity. It affects, even more, the appropriate content delivery. Thus, the objective of the presented tool is the treatment of Web pages taking into account the mentioned heterogeneity and adapting contents in order to improve the performance on the Web

A productivity map of cognitive pretest methods for improving survey questions

Resumen tomado de la publicaci??n

Inputs for the incorporation of the UNESCO guidelines on ICT competency standards for teachers : the training of teachers of mathematics in Central America

Resumen tomado de la publicaci??n

Implementación del Total Productive Management (TPM) como tecnología de gestión para el desarrollo de los procesos de MAQUIAVICOLA LTDA.

En el desarrollo de este trabajo de grado se describe la filosofía de calidad Total Productive Managment (TPM) y se desarrolla el plan de implementación de esta en una empresa del sector avícola colombiano conocida como MAQUIAVICOLA LTDA. Esta implementación tiene por objetivo optimizar la ejecución de los procesos productivos que se desarrollan dentro de la empresa con el fin de incrementar su productividad. A partir de la descripción de los conceptos y herramientas que contempla esta filosofía, se diseña un plan de implementación que va a permitir aplicar este modelo al desarrollo de los procesos diarios de la empresa, contribuyendo a la mejora de indicadores de gestión.

Análisis de los factores de productividad y talento humano como determinantes de la perdurabilidad en los grupos privados de investigación en salud

La perdurabilidad y la estrategia son temas que influyen de manera significativa en los sectores productivos. Los grupos de investigación, como eje principal de la ciencia, tecnología e innovación no son ajenos esto. Es así que requieren fortalecer su estructura logrando desempeño superior y entenderse como empresas. Esta investigación busca diseñar, para los factores de talento humano y productividad, un escenario deseado y sus estrategias de dirección para favorecer la perdurabilidad de los grupos privados de investigación en salud. La hipótesis planteada a partir de la cual se realizó el análisis es que los grupos de investigación privados, tienen mayores amenazas para perdurar porque no cuentan con una estructura propia de talento humano financiada o con soporte estructural de procesos para competir por las iniciativas de investigación disponibles. Esta hipótesis se aborda para los grupos privados de investigación que pertenecen al Programa Nacional de Ciencia y Tecnología de la Salud, área del conocimiento en ciencias de la salud y a través de la pregunta: ¿Cuáles pueden ser los escenarios posibles para lograr la perdurabilidad de los grupos privados de investigación en salud, a partir de estrategias relacionadas con los factores de talento humano y productividad? El desarrollo se hizo a través de 2 fases: Un estudio piloto donde se exploró el sector estratégico a través de las herramientas de prospectiva: MICMAC y SMIC y la fase de intervención a través de una metodología conjugada entre aplicación de AESE, un análisis DEA y una simulación de los escenarios encontrados y deseados.

Tools to Create a Poster Using PowerPoint 2010

This step-by-step guide introduces the PowerPoint 2010 features that can be used to create attractive posters. For example, guidelines to help elements align neatly and text boxes with margins.

Tools for Communication, Collaboration �and for Mobile Learning

Introduction as part of UG TEL course

Tools to use when writing a thesis in Word 2010/2013 (PC)

This collection of videos shows you how to use a range of time-saving tools when writing a thesis in MS Word 2010/2013. See the full SupportGuide at http://www.go.soton.ac.uk/thesispc. There are videos on using styles; creating tables of contents and tables of figures; using the Navigation Pane; using the Browse Object tool and many more. There is an equivelent collection for use with Word 2011 which is for use with Apple computers.

Tools to use when writing a thesis using Word 2011 (for Apple computers)

A collection of videos on time saving features when using MS Word 2011 to write a thesis. Learn how to use styles, make table of contents, make table of figures, use the document map, use the browse object tool and keep a count of the words in your file and many more useful features of Word 2011. Word 2011 is for Apple computers, there is a collection of similar video for use with the PC version Word 2010.

Tidying up tools for writing a thesis in MS Word 2010 (PC) Manual

This manual shows you how to use a range of useful smaller features Word 2010 - cross referencing, track changes, word count, endnotes and footnotes, spell checker, autocorrect, screen split, comments and find and replace.

Tidying up tools in MS Word 2011 (Mac) Manual

This manual shows you how to use a range of useful smaller features Word 2011 - cross-referencing, track changes, word count, endnotes and footnotes, spell checker, autocorrect, screen split, comments and find and replace.