990 resultados para atividade específica de esterase


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Pós-graduação em Biotecnologia - IQ

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This paper is the result of a research based on the school culture concept, which object is the specific school culture of the physical education (PE) within a school for youth and adults situated in Curitiba - PR. Methodologically we followed the principles and concepts of the ethnomethodology, that led us to a long immersion in the research field. The main results of the research show the relevance of the teacher identity for constituting the school culture of the PE, as well the paradox resulting of a mere theoretical approach: on the one hand this school culture show us the importance of the disruption with the PE's tradition based on the notion of "activity", and on the other hand reveal the necessity of the body experience as a fundament to build a theoretical construct

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This paper is the result of a research based on the school culture concept, which object is the specific school culture of the physical education (PE) within a school for youth and adults situated in Curitiba - PR. Methodologically we followed the principles and concepts of the ethnomethodology, that led us to a long immersion in the research field. The main results of the research show the relevance of the teacher identity for constituting the school culture of the PE, as well the paradox resulting of a mere theoretical approach: on the one hand this school culture show us the importance of the disruption with the PE's tradition based on the notion of "activity", and on the other hand reveal the necessity of the body experience as a fundament to build a theoretical construct

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This paper is the result of a research based on the school culture concept, which object is the specific school culture of the physical education (PE) within a school for youth and adults situated in Curitiba - PR. Methodologically we followed the principles and concepts of the ethnomethodology, that led us to a long immersion in the research field. The main results of the research show the relevance of the teacher identity for constituting the school culture of the PE, as well the paradox resulting of a mere theoretical approach: on the one hand this school culture show us the importance of the disruption with the PE's tradition based on the notion of "activity", and on the other hand reveal the necessity of the body experience as a fundament to build a theoretical construct

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Historically, man has empirically acquired knowledge about the therapeutic applications of extracted elements of the natural environment in which belonged. Such knowledge over time culminated in the formation of traditional health systems. Among its features, the use of bioactive plant species - medicinal plants - stands out for its efficiency and high popular acceptance. Despite its importance for public health, the population still has in the open-air fairs the main source for the acquisition of the species used. In these spaces, the trade generally occurs informally, under unfavorable conditions to the quality of the products and to the financial sustainability of the business. In this context, this study aimed to characterize the socioeconomic, cultural and sanitary aspects related to the trade of medicinal plants in municipalities of a semiarid region of Rio Grande do Norte, and additionally, proposing a specific legislation to the activity. Socioeconomic data were collected through on-site interviews, guided by structured form. The observations about the hygienic and sanitary adequacy of physical facilities and practices employed at the point of sale /environment were conducted and recorded with the use of assessment tool developed for use in open markets. The adequacy of medicinal plants to consumption was determined by microbiological analysis. The activity was carried out by individuals who are aged between 21 and 81 years of age, low educational level and low-income, predominantly males. The data showed a tendency to extinction of the activity in all the districts studied. It was observed in all the fairs studied hygiene and sanitation inadequacies that characterized very high health risk, representing in this way, the high probability of Food Transmitted Diseases outbreaks Such conditions were reflected in the high percentage of inadequacy to the consumption of the analyzed medicinal plants samples, illustrating the potential health risk to consumers. To contribute to the correction of hygiene and sanitation inadequacies observed in the studied open-air fairs, educational interventions were made to the training of traders in Good Practices. As a complement, was drafted a specific legislation for the marketing of folk medicine's products in open-air fairs. Such actions, products and its developments will contribute significantly to improving the quality of products available to the population and the preservation of activity, potentially reducing the risks to public health.

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This study involved the synthesis of photocatalysts based on titanium dioxide (TiO2). The photocatalysts were synthesized by the sol-gel method using three different proportions of acetone (25%, 50% and 75% v/v) in water/acetone mixtures, in order to control the hydrolysis of the precursor of titanium (titanium tetraisopropoxide). Aiming to investigate the structural, morphological and electronic changes provoked by the use of the solvent mixtures, different methodologies were used to characterize the oxides, such as X-ray diffraction (XRD), RAMAN spectroscopy, UV-Vis diffuse reflectance spectroscopy, and measurements of specific surface area (BET). XRD combined to RAMAN analyses revealed that the products are two-phase highly crystalline oxides involving anatase as main phase and brookite. Besides, the refined XRD using the method of Rietveld demonstrated that the presence of acetone during the synthesis influenced in the composition of the crystalline phases, increasing the proportion of the brookite phase between 13 and 22%. The band gap energy of these oxides practically did not suffer changes as function of the synthesis conditions. As shown by the isotherm, these photocatalysts are mesoporous materials with mean diameter of pores of 7 nm and approximately 20% of porosity. The surface area of the oxides prepared by hydrolysis in presence of acetone was 12% higher compared to the bare oxide. After characterized, these oxides had their photocatalytic activities evaluated by photodegradation of the azo dyes Ponceau 4R (P4R), Tartrazine (TTZ) and Reactive Red 120 (RR120), and also by the ability to mediate the photocatalytic production of hydrogen. Using the most efficient photocatalyst, the mineralization achieved for the dyes P4R, RR120 and TTZ was of respectively 83%, 79% and 56% in 120 minutes of reaction, while the discoloration of P4R e RR120 reached 100% and 94% for TTZ. In addition, the same photocatalyst in the presence of 0.5% w/w of Platinum and suspended in a 5:1 v/v water/methanol mixture, produced 56 mmol of gaseous hydrogen in five hours of experiment, corresponding to a specific rate of hydrogen production of 139.5 mmol h-1 g-1.

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Este estudo surgiu com o intuito de compreender se os mais velhos continuam, após a transição para a reforma, a participar na esfera da economia, formal e informal, e da sociabilidade, analisando com mais pormenor no que se refere a esta última faceta o sentimento de solidão. Partindo dos diferentes enquadramentos teóricos sobre o contributo socioeconómico dos idosos o objetivo foi compreender, no campo empírico, a posição dos sujeitos relativamente à transição para a reforma e de que forma os mesmos se percecionavam em áreas ligadas à produtividade e sociabilidade. A amostra foi intencional (N=30) e abrangeu sujeitos com idade igual ou superior a sessenta e cinco anos, com pelo menos um ano de reforma e residentes na Alta de Coimbra. A entrevista semiestruturada e a observação direta foram os instrumentos selecionados para aprofundar as vivências dos participantes. No que respeita ao tratamento dos dados recorremos à técnica da análise de conteúdo, com base em Bardin, que nos permitiu captar a singularidade de cada sujeito, sem descurar a totalidade das observações. No final concluiu-se que os sujeitos entrevistados vivenciam a transição para a reforma de maneiras distintas, reforçando assim a heterogeneidade deste grupo, e que são dotados de um capital social e económico que urge potencializar, quer no seio da sociedade civil, quer de forma específica no âmbito da Gestão de Recursos Humanos. / This study aims to understand if, after they retire, the elderly continue to get involved in areas like economy, formal and informal, and sociability, analyzing in detail the feeling of loneliness. Bearing in mind the different theories about the elderly’s social and economic contribution, the goal was to empirically understand the attitude of these individuals towards their transition to retirement, and to see how they perceive themselves and their role in productivity and sociability. The sample was intentional (N=30) and has included people aged sixty five and over sixty five, who have been retired for at least one year, and living in Alta de Coimbra. The semisstructured interview and direct observation were used to know the participants’ way of live. In what comes to data processing, we followed the content analysis technique, based on Bardin, which allows us to capture the singularity of each individual, without forgetting the whole set of observations. In the end we have concluded that the interviewed individuals live the transition into retirement in different ways, thus reinforcing the heterogeneity of this group and their social and economic capital, which needs to be put to use, both in civil society and more specifically in the sphere of the human management resources.

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Four different sponge species were screened using Ouchterlony agarose gel and immunodiffusion tests to identify cross-reactivity with the polyclonal antibody IgG anti-deglicosilated CvL, a lectin from Cliona varians. Crude extract from the sponge Cinachyrella apion showed cross-reactivity and also a strong haemmaglutinating activity towards human erythrocytes of all ABO groups. Thus, it was submitted to acetone fractionation, IgG anti-deglicosilated CvL Sepharose affinity chromatography, and Fast Protein Liquid Chromatography (FPLC-AKTA) gel filtration on a Superose 6 10 300 column to purify a novel lectin. C. apion lectin (CaL) agglutinated all types of human erythrocytes with preference for papainized type A and O erythrocytes. The haemagglutinating activity is independent of Ca2+, Mg2+ and Mn2+ ions, and it was strongly inhibited by the disaccharide D-lactose, up to a minimum concentration of 6.25 mM. CaL molecular mass determined by FPLC-AKTA gel filtration on a Superose 12 10 300 column and SDS gel electrophoresis was approximately 124 kDa, consisting of eight subunits of 15.5 kDa, assembled by hydrophobic interactions. The lectin was relatively heat- and pH-stable. Leishmania chagasi romastigotes were agglutinated by CaL, indicating that lactose receptors could be presented in this parasite stage. These findings are indicative of the physiological defense roles of CaL and its possible use in the antibiosis of pathogenic protozoa

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INTRODUÇÃO E OBJETIVOS: O fisioterapeuta é um profissional que presta serviços de saúde a indivíduos e populações com o objetivo de desenvolver, manter e restaurar o movimento e a capacidade funcional máxima ao longo da vida. Os programas de prevenção primária e secundária têm como principal objetivo a promoção da saúde, devendo centrar-se na consolidação da relação entre a saúde e os componentes fisiológicos da aptidão física relacionada com a saúde (ApFRS). O presente estudo teve como principal objetivo estimar a contribuição das caraterísticas individuais, dos componentes da ApFRS e da atividade física (AF) no estado de saúde de adultos jovens. Foram ainda objetivos do estudo avaliar a contribuição das caraterísticas individuais e de cada componente da ApFRS na ApFRS de adultos jovens e estudar as diferenças significativas entre os diferentes grupos género, tabagismo, hipertensão, dislipidémia, glicémia em jejum alterada, obesidade, sedentarismo, colesterol HDL elevado e estratificação do risco de doença cardiovascular (DCV) relativamente às variáveis correspondentes às caraterísticas individuais, estado de saúde e ApFRS. MATERIAL E MÉTODOS: Foram avaliados 146 adultos jovens (idade 20,1 ± 2,4 anos, peso 61,8 ± 10,5 kg, estatura 165,3 ± 7,4 cm). O protocolo de avaliação incluiu o preenchimento de um questionário de caraterização e dos questionários MOS Short Form Health Survey – 36 Item (SF-36) e o International Physical Activity Questionnaire (IPAQ). A bateria de testes para avaliação da ApFRS incluiu a avaliação da composição corporal: rácio cintura/anca (RCA) e percentagem de massa gorda (%MG); aptidão cardiorrespiratória (ACR): teste do degrau (TD); força muscular: força de preensão manual (FPM); resistência muscular: teste de força de braços (TFB) e teste de força abdominal (TFA) e flexibilidade: teste do sentar e alcançar (TSA). RESULTADOS: Embora a AF seja preditora da qualidade de vida relacionada com a saúde (QVRS), só por si não explica muitas das suas dimensões. Os componentes da ApFRS mostram ser melhores preditores do estado de saúde do que o nível de AF. A AF afecta a ApFRS, mas esta é muito mais influenciada pelas caraterísticas individuais e pela inter-relação dos diferentes componentes da ApFRS. O género, dislipidémia, glicémia em jejum alterada sedentarismo, colesterol HDL elevado e estratificação do risco de DCV têm um efeito significativo sobre as caraterísticas individuais, QVRS e ApFRS. CONCLUSÕES: O fisioterapeuta deve individualizar e personalizar cada programa de exercícios em função de cada indivíduo ou população específica, com ou sem patologia associada, uma vez que as caraterísticas individuais, ApFRS e AF interferem no tipo de exercícios indicados para cada indivíduo, e consequentemente nos resultados esperados do plano de exercícios prescrito.

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Dissertação de mestrado com vista à obtenção do Grau de Mestre em Educação Social e Intervenção Comunitária.

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Dissertação de mestrado com vista à obtenção do Grau de Mestre em Educação Social e Intervenção Comunitária.

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A partir de uma pesquisa histórica sobre a regulação da atividade petrolífera nacional, constatou-se que as jazidas de petróleo e de gás natural são bens públicos cuja exploração é constitucionalmente reservada ao Estado. Ademais, a delegação da exploração desses bens, por meio de concessão ou de partilha de produção, mantém a propriedade estatal desses recursos naturais.

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Consultoria Legislativa - Área XIX - Ciência Política, Sociologia Política, História, Relações Internacionais.

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Consultoria Legislativa - Área XI - Meio Ambiente e Direito Ambiental, Organização Territorial e Desenvolvimento Urbano e Regional.