Variation at Diabetes- and Obesity-Associated Loci May Mirror Neutral Patterns of Human Population Diversity and Diabetes Prevalence in India

South Asian populations harbor a high degree of genetic diversity, due in part to demographic history. Two studies on genome-wide variation in Indian populations have shown that most Indian populations show varying degrees of admixture between ancestral north Indian and ancestral south Indian components. As a result of this structure, genetic variation in India appears to follow a geographic cline. Similarly, Indian populations seem to show detectable differences in diabetes and obesity prevalence between different geographic regions of the country. We tested the hypothesis that genetic variation at diabetes-and obesity-associated loci may be potentially related to different genetic ancestries. We genotyped 2977 individuals from 61 populations across India for 18 SNPs in genes implicated in T2D and obesity. We examined patterns of variation in allele frequency across different geographical gradients and considered state of origin and language affiliation. Our results show that most of the 18 SNPs show no significant correlation with latitude, the geographic cline reported in previous studies, or by language family. Exceptions include KCNQ1 with latitude and THADA and JAK1 with language, which suggests that genetic variation at previously ascertained diabetes-associated loci may only partly mirror geographic patterns of genome-wide diversity in Indian populations.

De los yeux-partis franceses a los debates de la poesía cancioneril

Integran este número de la revista ponencias presentadas en Studia Hispanica Medievalia VIII : Actas de las X Jornadas Internacionales de Literatura Española Medieval, 2011, y de Homenaje al Quinto Centenario del Cancionero General de Hernando del Castillo.

De la métrica y versificación de las Cantigas de Santa María de Alfonso X, El Sabio, y la de los Cancioneros

Integran este número de la revista ponencias presentadas en Studia Hispanica Medievalia VIII : Actas de las X Jornadas Internacionales de Literatura Española Medieval, 2011, y de Homenaje al Quinto Centenario del Cancionero General de Hernando del Castillo.

Las relaciones entre texto y música en la producción del rey Don Denis de Portugal

Integran este número de la revista ponencias presentadas en Studia Hispanica Medievalia VIII: Actas de las IX Jornadas Internacionales de Literatura Española Medieval, 2008, y de Homenaje al Quinto Centenario de Amadis de Gaula.

Contrafactum y adaptación de la canción de Malmaridada en la Península Ibérica : del Cancionero del rey Don Denis al Cancionero para cantar la noche de Navidad de Francisco de Ocaña

Integran este número de la revista ponencias presentadas en Studia Hispanica Medievalia VIII : Actas de las X Jornadas Internacionales de Literatura Española Medieval, 2011, y de Homenaje al Quinto Centenario del Cancionero General de Hernando del Castillo.

Un caso de auto-reescritura : El astrólogo fingido de Calderón

Resumen: La pulcra edición de la Segunda parte de comedias de Pedro Calderón de la Barca por Santiago Fernández Mosquera (Biblioteca Castro, 2007) y la reciente tesis de Fernando Rodríguez-Gallego (2009) sobre las dos versiones de El astrólogo fingido (Zaragoza, 1632 y Madrid, 1637) obligan hoy a una revisión de uno de los casos, tan frecuentes en la producción del dramaturgo madrileño, de reescritura —con miras esta vez a la publicación impresa— de una de sus obras. El examen se centrará, más allá de consideraciones formales y estéticas, en la valoración del alcance ético de las profundas transformaciones sufridas por el personaje del primer protagonista, el falso astrólogo, don Diego de Luna, así como en el análisis de las dos versiones del desenlace.

Letras, 1985, nº 14 (número completo)

Contenido: El aparente estilo sin estilo en “El gallego y su cuadrilla”, de Camilo José Cela / Norma Susana Alioto – Notas para una evaluación del pensamiento de Bakhtin en el “Rabelais” / Susana G. Artal – La India y sus caminos de liberación / Carmen Balzer -- ¿Usó el moly Odiseo? / Pablo A. Cavallero – La historia y la novela: “El banquete de Severo Arcángelo”, de Leopoldo Marechal / Graciela Maturo – Las Confesiones de San Agustín, obra concluída, según el análisis de estructura y estilo / Amalia S. Nocito – El texto del Poema de Mio Cid ante el proceso de la tradicionalidad oral y escrita / Germán Orduna – La conciencia moral en una novela de H. James / Rosa E. M. D. Penna – El mundo medieval en la poesía de Enrique Banchs / Lía Noemí Uriarte Rebaudi – La construcción condicional en español con + infinitivo / Hilda Albano Vázquez – Ensayo etnológico de “La tierra permanece”, de George Stewart // Pablo Wright – Nota – Reseñas bibliográficas

Regulación alfa1-adrenérgica de la corriente IKr cardiaca

149 p. : il., graf.

Influencia de las bajas temperaturas en la supervivencia y el perfil proteico de Vibrio harveyi

Poster presentado al XXII Congreso Nacional de Microbiología celebrado en Salamanca los días 11-14 julio de 2011.

Introducción a los sistemas de cómputo paralelo

Duración (en horas): Más de 50 horas. Destinatario: Estudiante y Docente

Notas para una historia de la traducción literaria en Galicia

Eterio Pajares, Raquel Merino y José Miguel Santamaría (eds.)

Increased opioid dependence in a mouse model of panic disorder

[EN] Panic disorder is a highly prevalent neuropsychiatric disorder that shows co-occurrence with substance abuse. Here, we demonstrate that TrkC, the high-affinity receptor for neurotrophin-3, is a key molecule involved in panic disorder and opiate dependence, using a transgenic mouse model (TgNTRK3). Constitutive TrkC overexpression in TgNTRK3 mice dramatically alters spontaneous firing rates of locus coeruleus (LC) neurons and the response of the noradrenergic system to chronic opiate exposure, possibly related to the altered regulation of neurotrophic peptides observed. Notably, TgNTRK3 LC neurons showed an increased firing rate in saline-treated conditions and profound abnormalities in their response to met5-enkephalin. Behaviorally, chronic morphine administration induced a significantly increased withdrawal syndrome in TgNTRK3 mice. In conclusion, we show here that the NT-3/TrkC system is an important regulator of neuronal firing in LC and could contribute to the adaptations of the noradrenergic system in response to chronic opiate exposure. Moreover, our results indicate that TrkC is involved in the molecular and cellular changes in noradrenergic neurons underlying both panic attacks and opiate dependence and support a functional endogenous opioid deficit in panic disorder patients.

Multi-drug resistance profiles and the genetic features of Acinetobacter baumannii isolates from Bolivia

Introduction: Acinetobacter baumannii is opportunistic in debilitated hospitalised patients. Because information from some South American countries was previously lacking, this study examined the emergence of multi-resistant A. baumannii in three hospitals in Cochabamba, Bolivia, from 2008 to 2009. Methodology: Multiplex PCR was used to identify the main resistance genes in 15 multi-resistant A. baumannii isolates. RT-PCR was used to measure gene expression. The genetic environment of these genes was also analysed by PCR amplification and sequencing. Minimum inhibitory concentrations were determined for key antibiotics and some were determined in the presence of an efflux pump inhibitor, 1-(1-napthylmethyl) piperazine. Results: Fourteen strains were found to be multi-resistant. Each strain was found to have the bla(OXA-58) gene with the ISAba3-like element upstream, responsible for over-expression of the latter and subsequent carbapenem resistance. Similarly, ISAba1, upstream of the bla(ADC) gene caused over-expression of the latter and cephalosporin resistance; mutations in the gyrA(Ser83 to Leu) and parC (Ser-80 to Phe) genes were commensurate with fluoroquinolone resistance. In addition, the adeA, adeB efflux genes were over-expressed. All 15 isolates were positive for at least two aminoglycoside resistance genes. Conclusion: This is one of the first reports analyzing the multi-drug resistance profile of A. baumannii strains isolated in Bolivia and shows that the over-expression of thebla(OXA-58), bla(ADC) and efflux genes together with aminoglycoside modifying enzymes and mutations in DNA topoisomerases are responsible for the multi-resistance of the bacteria and the subsequent difficulty in treating infections caused by them.

Mechanisms Responsible for the Trophic Effect of Beta-Adrenoceptors on the I-to Current Density in Type 1 Diabetic Rat Cardiomyocytes

Background/Aims: In diabetic ventricular myocytes, transient outward potassium current (I-to) amplitude is severely reduced because of the impaired catecholamine release that characterizes diabetic autonomic neuropathy. Sympathetic nervous system exhibits a trophic effect on I-to since incubation of myocytes with noradrenaline restores current amplitude via beta-adrenoceptor (beta AR) stimulation. Here, we investigate the intracellular signalling pathway though which incubation of diabetic cardiomyocytes with the beta AR agonist isoproterenol recovers I-to amplitude to normal values. Methods: Experiments were performed in ventricular myocytes isolated from streptozotocin-diabetic rats. I-to current was recorded by using the patch-clamp technique. Kv4 channel expression was determined by immunofluorescence. Protein-protein interaction was determined by coimmunoprecipitation. Results: Stimulation of beta AR activates first a G alpha s protein, adenylyl cyclase and Protein Kinase A. PKA-phosphorylated receptor then switches to the G alpha i protein. This leads to the activation of the beta AR-Kinase-1 and further receptor phosphorylation and arrestin dependent internalization. The internalized receptor-arrestin complex recruits and activates cSrc and the MAPK cascade, where Ras, c-Raf1 and finally ERK1/2 mediate the increase in Kv4.2 and Kv4.3 protein abundance in the plasma membrane. Conclusion: beta(2)AR stimulation activates a G alpha s and G alpha i protein dependent pathway where the ERK1/2 modulates the Ito current amplitude and the density of the Kv4.2 and Kv4.2 channels in the plasma membrane upon sympathetic stimulation in diabetic heart.