Molecular evolution of two vertebrate aryl hydrocarbon (dioxin) receptors (AHR1 and AHR2) and the PAS family

The aryl hydrocarbon receptor (AHR) is a ligand-activated transcription factor through which halogenated aromatic hydrocarbons such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) cause altered gene expression and toxicity. The AHR belongs to the basic helix–loop–helix/Per-ARNT-Sim (bHLH-PAS) family of transcriptional regulatory proteins, whose members play key roles in development, circadian rhythmicity, and environmental homeostasis; however, the normal cellular function of the AHR is not yet known. As part of a phylogenetic approach to understanding the function and evolutionary origin of the AHR, we sequenced the PAS homology domain of AHRs from several species of early vertebrates and performed phylogenetic analyses of these AHR amino acid sequences in relation to mammalian AHRs and 24 other members of the PAS family. AHR sequences were identified in a teleost (the killifish Fundulus heteroclitus), two elasmobranch species (the skate Raja erinacea and the dogfish Mustelus canis), and a jawless fish (the lamprey Petromyzon marinus). Two putative AHR genes, designated AHR1 and AHR2, were found both in Fundulus and Mustelus. Phylogenetic analyses indicate that the AHR2 genes in these two species are orthologous, suggesting that an AHR gene duplication occurred early in vertebrate evolution and that multiple AHR genes may be present in other vertebrates. Database searches and phylogenetic analyses identified four putative PAS proteins in the nematode Caenorhabditis elegans, including possible AHR and ARNT homologs. Phylogenetic analysis of the PAS gene family reveals distinct clades containing both invertebrate and vertebrate PAS family members; the latter include paralogous sequences that we propose have arisen by gene duplication early in vertebrate evolution. Overall, our analyses indicate that the AHR is a phylogenetically ancient protein present in all living vertebrate groups (with a possible invertebrate homolog), thus providing an evolutionary perspective to the study of dioxin toxicity and AHR function.

Micro-meso-macro

Building on the ontology of evolutionary realism recently proposed by Dopfer and Potts (forthcoming), we develop an analytical framework for evolutionary economics with a micro-meso-macro architecture. The motive for reconception is to make clear the highly complex and emergent nature of existence and change in economic evolution. For us, the central insight is that an economic system is a population of rules, a structure of rules, and a process of rules. The economic system is a rule-system contained in what we call the meso. From the evolutionary perspective, one cannot directly sum micro into macro. Instead, we conceive of an economic system as a set of meso units, where each meso consists of a rule and its population of actualizations. The proper analytical structure of evolutionary economics is in terms of micro-meso-macro. Micro refers to the individual carriers of rules and the systems they organize, and macro consists of the population structure of systems of meso. Micro structure is between the elements of the meso, and macro structure is between meso elements. The upshot is an ontologically coherent framework for analysis of economic evolution as change in the meso domain - in the form of what we call a meso trajectory - and a way of understanding the micro-processes and macro-consequences involved. We believe that the micro-meso-macro analytical framework can greatly enhance the focus, clarity, and, ultimately, power, of evolutionary economic theory.

Testing the energetic equivalence rule with helminth endoparasites of vertebrates

As a general test of the energetic equivalence rule, we examined macroecological relationships among abundance, density and host body mass in a comparative analysis of the assemblages of trophically transmitted endoparasitic helminths of 131 species of vertebrate hosts. Both the numbers and total volume of parasites per gram of host decreased allometrically with host body mass, with slopes roughly consistent with those expected from the allometric relationship between host basal metabolic rate and body mass. From an evolutionary perspective, large body size may therefore allow hosts to escape from the deleterious effects of parasitism.

Data compilation on the biological response to ocean acidification: environmental and experimental context of data sets and related literature

The exponential growth of studies on the biological response to ocean acidification over the last few decades has generated a large amount of data. To facilitate data comparison, a data compilation hosted at the data publisher PANGAEA was initiated in 2008 and is updated on a regular basis (doi:10.1594/PANGAEA.149999). By January 2015, a total of 581 data sets (over 4 000 000 data points) from 539 papers had been archived. Here we present the developments of this data compilation five years since its first description by Nisumaa et al. (2010). Most of study sites from which data archived are still in the Northern Hemisphere and the number of archived data from studies from the Southern Hemisphere and polar oceans are still relatively low. Data from 60 studies that investigated the response of a mix of organisms or natural communities were all added after 2010, indicating a welcomed shift from the study of individual organisms to communities and ecosystems. The initial imbalance of considerably more data archived on calcification and primary production than on other processes has improved. There is also a clear tendency towards more data archived from multifactorial studies after 2010. For easier and more effective access to ocean acidification data, the ocean acidification community is strongly encouraged to contribute to the data archiving effort, and help develop standard vocabularies describing the variables and define best practices for archiving ocean acidification data.

Filogenia molecular das enzimas isocitrato liase e malato sintase e sua evolução em Viridiplanta

The plant metabolism consists of a complex network of physical and chemical events resulting in photosynthesis, respiration, synthesis and degradation of organic compounds. This is only possible due to the different kinds of responses to many environmental variations that a plant could be subject through evolution, leading also to conquering new surroundings. The glyoxylate cycle is a metabolic pathway found in glyoxysomes plant, which has unique role in the seedling establishment. Considered as a variation of the citric acid cycle, it uses an acetyl coenzyme A molecule, derived from lipids beta-oxidation to synthesize compounds which are used in carbohydrate synthesis. The Malate synthase (MLS) and Isocitrate lyase (ICL) enzyme of this cycle are unique and essential in regulating the biosynthesis of carbohydrates. Because of the absence of decarboxylation steps as rate-limiting steps, detailed studies of molecular phylogeny and evolution of these proteins enables the elucidation of the effects of this route presence in the evolutionary processes involved in their distribution across the genome from different plant species. Therefore, the aim of this study was to establish a relationship between the molecular evolution of the characteristics of enzymes from the glyoxylate cycle (isocitrate lyase and malate synthase) and their molecular phylogeny, among green plants (Viridiplantae). For this, amino acid and nucleotide sequences were used, from online repositories as UniProt and Genbank. Sequences were aligned and then subjected to an analysis of the best-fit substitution models. The phylogeny was rebuilt by distance methods (neighbor-joining) and discrete methods (maximum likelihood, maximum parsimony and Bayesian analysis). The identification of structural patterns in the evolution of the enzymes was made through homology modeling and structure prediction from protein sequences. Based on comparative analyzes of in silico models and from the results of phylogenetic inferences, both enzymes show significant structure conservation and their topologies in agreement with two processes of selection and specialization of the genes. Thus, confirming the relevance of new studies to elucidate the plant metabolism from an evolutionary perspective

O comportamento pró-social de crianças com sintomatologia do transtorno da conduta

Pro-social behaviors are seen regularly throughout our daily lives, as we often witness people giving alms, helping a neighbor move, donating blood, or taking care of a friend's children, among others. From an evolutionary perspective, such behaviors occur because they have a high adaptive value to our species, precisely due to our high degree of dependence on group living for survival. Probably, for this same reason, since children have shown a preference for prosocial behaviors over antisocial behaviors, this preference becomes more visible as we grow. However, children with symptoms of conduct disorder show a pattern of aggressive, impulsive and more selfish behaviors than children without such symptoms. Furthermore, these children also experience environments in which antisocial behaviors are more frequent and intense compared to the general population. Priming experiments are one way of measuring the influence of simple environmental cues on our behavior. For example, driving faster when listening to music, religious people help more on religious elements, like the bible, and children are more cooperative after playing games of an educational nature. Thus, the objectives of the current study were to: evaluate whether there is any difference in generosity, through sharing behavior, among children with and without symptoms of conduct disorder; analyze the influence of prosocial priming on sharing behavior on children with and without symptoms of conduct disorder; and finally, analyze from an evolutionary perspective, the reasons given by children with and without symptoms of conduct disorder for sharing or not sharing with their best friend in a classroom environment. To address this question, the teachers of these children were asked to respond to an inventory that was designed to signal the presence or absence of symptoms of conduct disorder. Children identified as having or not having symptoms of conduct disorder could then undergo an experimental (with priming) or control (no priming) condition. Under the experimental condition, the children were asked to watch two short videos showing scenes of helping and sharing among peers, to perform a distraction activity, and finally to chose two of four different materials presented by the researcher and decide how much of these two materials they would like to share with their best friend in the classroom. Then the children were asked about their reasons for sharing or not sharing. Children subjected to the control condition performed the same activities as in the xi experimental condition, but did not watch the video first. The results showed a notable difference in the effect of priming in accordance with the child's stage of development; a difference in the amount of material donated to a best friend by children with and without symptoms of conduct disorder, and a change in this observed difference with the influence of pro-social priming; and finally, a convergence in the thinking of children regarding their reasons for sharing with evolutionary theory. The results of this study also indicate the importance of individual factors, developmental stage, environmental and evolutionary conditions in the pro-social behavior of children with and without symptoms of conduct disorder.

La croyance au Père Noël a une date de péremption

La plupart des enfants en Occident sont en contact avec le Père Noël au cours de leur enfance. Pour la plupart des parents, il s’agit d’une croyance anodine destinée à conférer à la fête de Noël un certain mystère. Certains parents considèrent toutefois que promouvoir la croyance au Père Noël, c’est valoriser le mensonge. L’objectif de ce texte est de passer en revue les arguments des uns et des autres. Nous mettons d’abord en évidence quelques variables susceptibles de favoriser ou non cette croyance. Par la suite, nous présentons les travaux qui se sont intéressés à l’impact de la croyance au Père Noël et de la découverte de la vérité. Nous considérerons alors le point de vue des enfants et celui des parents. Nous évoquons enfin le caractère adaptatif du mensonge dans une perspective évolutionniste.

La croyance au Père Noël a une date de péremption

La plupart des enfants en Occident sont en contact avec le Père Noël au cours de leur enfance. Pour la plupart des parents, il s’agit d’une croyance anodine destinée à conférer à la fête de Noël un certain mystère. Certains parents considèrent toutefois que promouvoir la croyance au Père Noël, c’est valoriser le mensonge. L’objectif de ce texte est de passer en revue les arguments des uns et des autres. Nous mettons d’abord en évidence quelques variables susceptibles de favoriser ou non cette croyance. Par la suite, nous présentons les travaux qui se sont intéressés à l’impact de la croyance au Père Noël et de la découverte de la vérité. Nous considérerons alors le point de vue des enfants et celui des parents. Nous évoquons enfin le caractère adaptatif du mensonge dans une perspective évolutionniste.

A internacionalização de PME: o caso da Carvalheira, Bastos Lda.

Atualmente, a internacionalização é uma estratégia adotada por muitas empresas, independentemente da sua dimensão com o intuito de enfrentar o abrandamento económico no seu mercado doméstico e impulsionar o seu crescimento. No caso das PME, as principais razões que as levam a sair do seu mercado doméstico e a arriscar em mercados externos prendem-se com motivações reativas e muitas vezes exógenas, como sejam a saturação do mercado doméstico, as pressões da concorrência e as relações com os clientes. Assim, a crise financeira no país, aliada à crise no setor da construção civil, leva esta PME portuguesa, do ramo da carpintaria de limpo, a procurar novas oportunidades no exterior. No entanto, como tem consciência da falta de experiência e conhecimento nos mercados internacionais, começa por abordar os mercados numa lógica de procura de novas oportunidades, aproveitando as oportunidades que lhe são dirigidas e vendo nestas uma forma de aprofundar relacionamentos e ganhar experiência neste âmbito. Posteriormente, este conhecimento e experiência permitir-lhe-á enveredar por uma estratégia de internacionalização mais ativa, abandonando/diminuindo a lógica de oportunidade e apostando numa lógica mais estruturada e planeada. Este trabalho, ao tirar proveito de um único estudo de caso, pretende analisar a perspetiva evolutiva da empresa, e como uma estratégia empreendedora, a confiança e a incorporação em redes de relacionamentos facilitam o processo de abertura aos mercados externos.

Peripheral communities of the Eastern Lau Spreading Center and Valu Fa Ridge: community composition, temporal change and comparison to near-vent communities

Western Pacific hydrothermal vents will soon be subjected to deep-sea mining and peripheral sites are considered the most practical targets. The limited information on community dynamics and temporal change in these communities makes it difficult to anticipate the impact of mining activities and recovery trajectories. We studied community composition of peripheral communities along a cline in hydrothermal chemistry on the Eastern Lau Spreading Center and Valu Fa Ridge (ELSC-VFR) and also studied patterns of temporal change. Peripheral communities located in the northern vent fields of the ELSC-VFR are significantly different from those in the southern vent fields. Higher abundances of zoanthids and anemones were found in northern peripheral sites and the symbiont-containing mussel Bathymodiolus brevior, brisingid seastars and polynoids were only present in the northern peripheral sites. By contrast, certain faunal groups were seen only in the southern peripheral sites, such as lollipop sponges, pycnogonids and ophiuroids. Taxonomic richness of the peripheral communities was similar to that of active vent communities, due to the presence of non-vent endemic species that balanced the absence of species found in areas of active venting. The communities present at waning active sites resemble those of peripheral sites, indicating that peripheral species can colonize previously active vent sites in addition to settling in the periphery of areas of venting. Growth and mortality were observed in a number of the normally slow-growing cladorhizid stick sponges, indicating that these animals may exhibit life history strategies in the vicinity of vents that differ from those previously recorded. A novel facultative association between polynoids and anemones is proposed based on their correlated distributions.

O serviço de referência nas bibliotecas públicas em Portugal: modelo de desenvolvimento

O Serviço de Referência (SR), incorporando na sua organização, funcionamento e comunicação com o utilizador um conjunto de potencialidades proporcionadas pela tecnologia, assume um papel fundamental na luta contra a desigualdade e o analfabetismo digital, no fomento da literacia da informação e na criação de conhecimento. A percepção do valor e das fragilidades do SR nas bibliotecas públicas em Portugal levanta a questão sobre como contribuir para o seu desenvolvimento. A presente investigação, visando a construção de alicerces numa perspectiva de evolução, parte da análise de documentos orientadores internacionais da RUSA/ALA (Reference and User Services Association / American Library Association) e da IFLA (International Federation of Library Association) e da observação de características estruturais da Rede Nacional de Biblioteca Públicas (RNBP), para a concepção de soluções de desenvolvimento do SR a aplicar a esta realidade concreta. Seguindo um paradigma compreensivo indutivo, com uma orientação baseada na Grounded Theory, esta pesquisa privilegia o contexto da descoberta e a construção de uma teoria. No plano empírico cumpre uma complementaridade de técnicas qualitativas e quantitativas, com recurso a uma triangulação metodológica. O campo de análise abrange a totalidade das bibliotecas públicas portuguesas integradas na RNBP em Portugal. Visando aspectos funcionais e materiais, são analisados dados preexistentes e aplicadas técnicas de observação directa e inquérito por questionário. Visando aspectos sociais, são efectuadas seis entrevistas semi-estruturadas. Os resultados apontam para uma situação de estagnação das bibliotecas da RNBP e para um funcionamento não estruturado e informal do SR, na maioria dos casos em que se verifica a sua disponibilização. Fundamentada a necessidade de evolução, e do investimento que nele possa ser feito, é construído um modelo, seguindo uma estratégia por níveis, flexíveis e adaptáveis às necessidades e aos recursos de cada biblioteca, que visa o desenvolvimento incremental e congruente daquele serviço; Abstract: The Reference Service in public libraries in Portugal. Development model from international guidelines. Incorporating in its organization, procedures and communication with the users a set of tools brought by technology, Reference Service (RS) plays a key role in the struggle against inequality and digital illiteracy, promoting information literacy and creating knowledge. The perception of the value of RS and the weaknesses in public libraries in Portugal raise the question on how to contribute to its development. In order to build RS foundations on an evolutionary perspective, this research analyzes RUSA / ALA (Reference and User Services Association / American Library Association) and IFLA (International Federation of Library Association) international guidelines and the structural characteristics of the Portuguese National Public Library Network (RNBP), with the objective of creating a solution for the development of that service to be applied to this reality. Following an inductive comprehensive paradigm, with an orientation based on Grounded Theory, this research focuses on the context of discovery and the construction of a theory. Empirically it fulfils complementary qualitative and quantitative techniques, using a methodological triangulation. The field of analysis covers Portuguese public libraries integrating the RNBP. Aiming at functional and material aspects, direct observation techniques and a survey by questionnaire are applied. Targeting social aspects, six semistructured interviews are carried out. The results point to a situation of stagnation in the RNBP as well as the unstructured and informal performance of the SR, which justifies the need for evolution and investment. A model is constructed, following a strategy based on levels, flexible and adaptable to the needs and resources of each library, with the perspective of an incremental and consistent development of this service.

O imposto sobre as transações financeiras : uma iniciativa europeia não consensual

Mestrado em Contabilidade, Fiscalidade e Finanças Empresariais

Molecular biology of prolactin and prolactin receptor in a marine teleost: the sea bream(Sparus aurata)

Tese de dout. em Biologia, especialidade de Biologia Molecular, Unidade de Ciências e Tecnologias dos Recursos Aquáticos, Univ. do Algarve

Características preferidas por hombres y mujeres que buscan pareja estable de su mismo sexo

Las preferencias en elección de pareja de hombres y mujeres interesados en establecer relaciones a largo plazo con personas de su mismo sexo biológico ha sido un tema de interés para el análisis evolucionista, ya que existe debate frente a los resultados de las investigaciones y los planteamientos de las teorías de inversión parental y estrategias sexuales. Con el objetivo de identificar qué características son preferidas por hombres y mujeres interesados en establecer una relación a largo plazo con personas de su mismo sexo biológico y contribuir a esta discusión, se llevó a cabo un estudio descriptivo en el que analizamos el contenido de 732 perfiles de hombres y mujeres (H=491; M=241) que buscaban una relación estable con parejas de su mismo sexo biológico. Las categorías analizadas fueron: edad, atractivo físico (apariencia, contextura, estatura y peso), estatus socioeconómico (situación laboral, nivel educativo y zona de residencia buscada), estado civil, número de hijos y hábitos saludables (fumar y beber). Los resultados encontrados muestran que los hombres presentan rangos amplios en las características deseadas en una pareja (edad=16.87; estatura=11.37; peso=15.23) y además buscan personas menores a la edad ofrecida (M=-4.17 años). En las mujeres se encontró que los rangos son más restringidos (edad=13.85; estatura=9.83; peso=12.77) y además prefieren parejas mayores (M=2.89 años). A nivel general, se evidencia que los resultados encontrados en la mayoría de las variables podrían indicar congruencia con los planteamientos de la teoría de inversión parental y estrategias sexuales; sin embargo, en otras variables los resultados no son claros.

El rol de las redes de apoyo transnacional en la evolución del régimen de trata de personas en la región del mekong, 2004-2015

Con el fin de la unipolaridad no sólo se fortalecieron mecanismos de gobernanza global como los Regímenes Internacionales, sino también se fortalecieron actores no estatales. A pesar de la importancia que tomaron estos dos elementos aún no existe una teoría que explique exhaustivamente la relación que existe entre ellos. Es por lo anterior que, la investigación busca responder de qué manera el rol de las Redes de Apoyo Transnacional ha incidido en la evolución del régimen de tráfico de personas en la Región del Mekong. Asimismo tiene como objetivo comprender las relación entre el Régimen y las Redes de Apoyo Transnacional a través de la formulación de un caso de estudio basado en metodologías cualitativas, específicamente, en el análisis teórico-constructivista y el análisis de contenido de documentos producidos por actores estatales y no estatales.