Avaliação da influência de surfactantes químico e biológico na hidrólise enzimática de casca de coco verde após pré-tratamento ácido/alcalino e com peróxido de hidrogênio alcalino

In Brazil many types of bioproducts and agroindustrial waste are generated currently, such as cacashew apple bagasse and coconut husk, for example. The final disposal of these wastes causes serious environmental issues. In this sense, waste lignocellulosic content, as the shell of the coconut is a renewable and abundant raw material in which its use has an increased interest mainly for the 2nd generation ethanol production. The hydrolysis of cellulose to reducing sugars such as glucose and xylose is catalysed by a group of enzymes called cellulases. However, the main bottleneck in the enzymatic hydrolysis of cellulose is the significant deactivation of the enzyme that shows irreversible adsorption mechanism leading to reduction of the cellulose adsorption onto cellulose. Studies have shown that the use of surfactants can modify the surface property of the cellulose therefore minimizing the irreversible binding. The main objective of the present study was to evaluate the influence of chemical and biological surfactants during the hydrolysis of coconut husk which was subjected to two pre-treatment in order to improve the accessibility of the enzymes to the cellulose, removing this way, part of the lignin and hemicellulose present in the structure of the material. The pre-treatments applied to coconut bagasse were: Acid/Alkaline using 0.6M H2SO4 followed by 1M NaOH, and the one with Alkaline Hydrogen Peroxide at a concentration of 7.35% (v/v) and pH 11.5. Both the material no treatment and pretreated were characterized using analysis of diffraction X-ray (XRD), Scanning Electron Microscopy (SEM) and methods established by NREL. The influence of both surfactants, chemical and biological, was used at concentrations below the critical micelle concentration (CMC), and the concentrations equal to the CMC. The application of pre-treatment with coconut residue was efficient for the conversion to glucose, as well as for the production of total reducing sugars, it was possible to observe that the pretreatment fragmented the structure as well as disordered the fibers. Regarding XRD analysis, a significant increase in crystallinity index was observed for pretreated bagasse acid/alkali (51.1%) compared to the no treatment (31.7%), while that for that treated with PHA, the crystallinity index was slightly lower, around 29%. In terms of total reducing sugars it was not possible to observe a significant difference between the hydrolysis carried out without the use of surfactant compared to the addition of Triton and rhamnolipid. However, by observing the conversions achieved during the hydrolysis, it was noted that the best conversion was using the rhamnolipíd for the husk pretreated with acid/alkali, reaching a value of 33%, whereas using Triton the higher conversion was 23.8%. The coconut husk is a residue which can present a high potential to the 2nd generation ethanol production, being the rhamonolipid a very efficient biosurfactant for use as an adjuvant in the enzymatic process in order to act on the material structure reducing its recalcitrance and therefore improving the conditions of access for enzymes to the substrate increasing thus the conversion of cellulose to glucose.

Obtenção de concentrado proteico do farelo de arroz e avaliação das propriedades químicas e funcionais

Esta dissertação é composta por 5 artigos.

Obtenção de polímeros de LCC para aplicação como antioxidante de biodiesel de soja

Biodiesel is a fuel obtained from vegetable oils, such as soy, castorbean, among others. The monoester of fatty acid of these oils have chains with mono, di and tri double connections. The presence of these insaturations are susceptible to oxidization. Antioxidants are substances able to prevent oxidization from oils, fats, fat foods, as well as esters of Alquila( biodiesel). The objective of this work is to summarize a new antioxidant from the Cashew Nut Shell Liquid (CNSL) using the electrolysis technique. A current of 2 amperes was used in a single cell of only one group and two eletrodos of stainless steel 304 in a solution of methanol, together with the eletrolits: acetic acid, sodium chloride and sodium hydroxide, for two hours of agitation. The electrolysis products are characterized by the techniques of cromatography in a thin layer, spectroscopy of infrared and gravimetric analysis. The material was submitted to tests of oxidative stability made by the techniques of spectropy of impendancy and Rancimat (EN 14112). The analyses of characterization suggest that the polimerization of the electrolytic material ocurred. The application results of these materials as antioxidants of soy biodiesel showed that the order of the oxidative stability was obtained by both techniques used

Carbon and nitrogen stocks of an arenosol under irrigated fruit orchards in semiarid Brazil.

O acúmulo de matéria orgânica beneficia a produtividade do solo e reduz a concentração do carbono atmosférico. Entretanto, pouco é sabido a respeito do acúmulo de C e N em solos arenosos cultivados com culturas perenes irrigadas em regiões semi-áridas. O objetivo deste trabalho foi avaliar o efeito do cultivo de fruteiras irrigadas nos estoques de C e N de um Neossolo Quartzarênico em Paraipaba, CE. Foram coletadas amostras na linha e na entrelinha de pomares irrigados de banana, caju, manga, sapoti, goiaba e graviola. Em áreas adjacentes sob vegetação de Caatinga hipoxerófila e após dois anos de desmatamento foram coletadas amostras adicionais. As profundidades de amostragem foram: 0 a 0.1, 0.1 a 0.2 e 0.2 a 0.4 m. Os teores de C e N no solo foram mais altos na camada superficial (0 a 0.1 m). Não houve efeito da profundidade de amostragem sobre a densidade do solo. Os estoques de C e N na área com vegetação de Caatinga na camada de 0 a 0.4 m foram de 27,6 and 2,4 Mg ha-1, respectivamente. A retirada da vegetação natural e o cultivo das fruteiras levaram a reduções de 5 a 23% e 4 a 21% nos estoques de C e N do solo, respectivamente. As culturas do sapoti e da graviola contribuíram para o aumento dos estoques de C e N após a retirada da vegetação natural. As culturas da goiaba, graviola, manga e sapoti contribuíram para a melhoria do índice de estratificação deste solo.

Heritability, repeatability, and genetic gains in a improvement population of cajuízeiro.

Cajuí (Anacardium spp) is an endemic fruit trees species in the Northeastern region of Brazil. Thus, this trial aimed to estimate the repeatability coefficients of the agrotechnological variables and to predict the genetic gains of a cajuí improvement population located at the Embrapa Meio-Norte.