Avaliação de seleções mutantes de figueira cv. Roxo-de-Valinhos

A figueira (Ficus carica L.), pertencente à família das Moráceas, constitui-se numa das mais importantes frutíferas cultivadas, elevando o Brasil à condição de décimo maior produtor e exportador de figos do mundo. Porém, a ficicultura apresenta alguns problemas fitossanitários, além de, no Brasil, estar toda implantada com uma única cultivar, a Roxo-de-Valinhos, que produz frutos sem sementes, inviabilizando o melhoramento convencional. Nesse sentido, o melhoramento genético, com o uso de mutagênicos, passa a ser uma linha de pesquisa altamente importante, podendo contribuir enormemente para o desenvolvimento da cultura. Diante disto, o objetivo do presente trabalho foi avaliar seleções mutantes de figueira originadas de plantas formadas por estacas provenientes de gemas irradiadas com raio gama, bem como compará-las com as principais cultivares da cultura em plantio comercial. Utilizou-se de plantas originadas de estacas de 5 cultivares tradicionais de figueira e de cinco seleções de figueiras mutantes (formadas por estacas originadas de gemas da cultivar Roxo-de-Valinhos irradiadas com raio gama, na dose de 30 Gy), cultivadas em espaçamento de 2,5 x 1,5 m. O delineamento experimental utilizado foi o de blocos inteiramente casualizados, contendo 10 tratamentos, com 3 repetições e 5 plantas por parcela. As avaliações foram realizadas a partir do desenvolvimento vegetativo das plantas, bem como as características dos frutos. da análise dos dados, conclui-se que há variabilidade entre as seleções mutantes e destas com as cultivares tradicionais, e que o tratamento PI-189 foi superior à cultivar-padrão Roxo-de-Valinhos em características de grande importância comercial, como número de frutos por planta, massa média por fruto e produtividade, igualando-se nas demais características, mostrando-se, assim, com enorme potencial para posteriores estudos.

O Turismo como um tema de pesquisa: um diálogo epistemológico a partir das produções dos programas de pós-graduação stricto sensu da Universidade Federal do Rio Grande do Norte, no período de 2007 a 2011

The study consists in an analysis of researches with tourism issues, in Master s and Doctoral level, investigating their epistemological trends, regarding the type of study that is being produced and the methods used. It is characterized as exploratory, documental and bibliographical, having undertaken a cross-sectional study which covers the period from 2007 to 2011. It analyzes the context of academically production in tourism, the nature of these researches, the methodological aspects adopted in these theses and dissertations, the philosophical inclinations and the coherence and contribution of the same. This research uses, the Digital Library of Theses and Dissertations (BDTD) and the secretariats of the Academic Centers of the Federal University of Rio Grande do Norte (UFRN) as means to collect data. It employs the technique of sampling by judgment and undertakes the research in various Academic Centers in UFRN, using the method of content analysis. It shows that, from the 643 studies analyzed, 43 of them have themes related to tourism, which characterized this study sample. This study realizes that PPGA is the largest producer of researches on tourism issues, ahead of PPGTur, which ranks the third position, in this aspect. Also realizes that all surveys analyzed are crosssectional and the theme further investigated, with 21% of the sample, is related to the marketing aspects of tourism, with the theme Directors of Hotels and Restaurants (DHR) . This research demonstrates that all sample surveys make use of interviews, and these are, mostly, structured. Also realizes that most of these studies, with 21% of the sample, employ the method of content analysis. The references in Portuguese are surveyed about five times more than the ones in other languages. It estimates that more than half of the sample is characterized as positivist, associated with aspects of functionalism. A minority of 21% of these researches seeks to identify and recommend solutions to the tourism issues, or even represent part of them. This research concludes that the studies in tourism are mostly unifocal, being driven by issues related to the touristic trade. PPGTur produces a number still unrepresentative of studies. The theses and dissertations of the sample are limited and/or unable to analyze the changes of the phenomenon caused by the time. The studies from the sample present criteria and make use of other methods and techniques to associate with the interviews and content analysis to obtain more solid and reliable data and results. They use a low number of references in languages other than Portuguese. Most of these studies, characterized as positivist-functionalist, investigate the functioning, planning, coordination and expectations in organizations, leaving aside other issues, also relevant for tourism. The number of studies aimed at identifying solutions to the problems of tourism, or that even represents part of them, is not expressive

O turismo de base local como um possível indutor de desenvolvimento rural: uma análise da realidade e das potencialidades no distrito de dondo-moçambique

The current debate on rural development focuses on the territorial approach, recognizing the multifunctional character of rural areas. Emphasize the use of endogenous forces, the need of implement policies which valorize local specificities, the participation of social actors in revitalization of rural areas. In this context tourism enters as a means to induce a new dynamic to rural areas, since the activity is regarded as a channel for the upgrading of the natural and cultural resources and the rural way of life. This study focuses on local-based tourism as a promoter of rural development, giving an analysis of the social reality and potential for this tourism in the district of Dondo. In it, is proposed to analyze the potential for development of local-based tourism in the district of Dondo, assessing the extent to which local reality enables the inclusion of the community in the management of local tourism resources. For this, was showed the inclusion of local-based tourism in the current context of rural development, was identified and organized systematically the factors that influence the implementation of local-based tourism in the study area and was verified as far as possible the study area corresponds to the constraints identified, showing how local-based tourism can best been encouraged. Was adopted a qualitative methodological approach to both the procedures and data processing, but also by critical approach, using bibliographical research, semi-structured interviews that fell on the group of public administrators who work in tourism and community leaders. The critical analysis was based on the principles of sustainability o Sachs particular ecological, social, cultural, economic, space, beyond the analysis of political and institutional aspects. The survey results show that the district of Dondo has a potential for implementing a model of tourism development on a local basis, since it is rich in natural and cultural research, benefits from good network accessibility in the context of the market in the region, the population live according to their habits and customs and value their identity. There is also a good cooperation between community members, although not in tourism, a fact which is observed through the lever of community participation in associations, cooperatives, working in areas such as agriculture, fishing, environment, civic education etc. It also has public funding sources to augment or stimulate local investment. Despite obstacles such as inexistence of policies and local plans for tourism development, lack of awareness of tourism, lack of basic infrastructure, as well as equipment and facilities to support tourism, the results shows that these problems can be solved through a public commitment from both government, and community through a joint planning and participation

Turismo e economia solidária : uma análise nas cooperativas e associações de artesanato do Roteiro Seridó norte-rio-grandense

The present study has addressed the issues surrounding the Solidarity Economy and Tourism, their perspectives and contributions to the development process of local communities, as well as the connection point between the two. This study evaluated the extent to which social economy through cooperatives and associations in tourism has generated socio-economic improvements for the artisans of the tourist Seridó. The study made as to their objectives, was exploratory and descriptive, since it involved both standard techniques of data collection, questionnaires and systematic observation, and secondary research and case studies, which characterizes an exploratory research, according to Castro (2008 ). The results indicate significant improvements offered by the inclusion of members in groups (associations and cooperatives) in matters concerning health, education, interpersonal relationships, and access to consumer credit to the artisans. Through this study, one might also note that the inclusion of products and services in tourism is not a relevant factor for the socio-economic improvements observed for the elevated presence of middlemen in the marketing process

O sensemaking e o Comitê Regional das Associações e Cooperativas do Artesanato Seridoense :buscando compreender um complexo de relações

The generation of direction, sensemaking, is the process where the actors start to perceive the events around them establishing and creating meanings in their actions that they play daily. At the moment where they happen the interactions in a net business-oriented between the actors are that sensemaking is generated. A business-oriented example of a relationship net is the work developed for the Committee of Associations and Regional Cooperatives of Handcraft of Seridó - CRACAS. This organization, with headquarters in the city of Caicó - Rio Grande of North exists with the objective to manage the net of craftsmen of the region of the norteriograndense Seridó. The present inquiry had as objective generality to understand the generation of sensible of the activities carried through for the organizational, inserted actors in the CRACAS in Caicó/RN, directed toward the use of the resources in its daily business-oriented. The specific objectives had been) To identify the resources used for the organizational actors of the CRACAS; b) To apprehend the way for which the craftsmen play activities directed toward the control and use of the available resources in its environment business-oriented; c) To identify the role played for the organizational actors (craftsmen) in its business-oriented daily activities; d) To understand as the organizational actors of the CRACAS they generate sensible of its business-oriented activities and finally e) To verify the principles that guide the interactions of the craftsmen. Of this process of empirical inquiry, the methodology used in the inquiry consisted of a Study of Case in the CRACAS and the seven Associations of the Embroidering it. It was concluded that sensemaking happens during the accomplishment of the activity as during the confection of the products. In accordance with craftsman the financial resources do not come from the CRACAS. One evidenced that the seven cities of the business-oriented embroidering interact as a net of the handcraft and that it exists a lack of resources and infrastructure in the associations

Estratégias de Produção in vitro de Bioinseticida Viral: influências do Isolado, da Cinética e do Modo de operação

Societal concerns about environmental sustainability has lead to the development of ecologically-friendly alternatives to chemical insecticides for crop protection. One such alternative is biological pest control. In particular, baculoviruses are well suited as insect biopesticides due to their narrow host specificity and relative ease of propagation. In Brazil, the baculovirus Anticarsia gemmatalis nucleopolyhedrovirus (AgMNPV) is the main biological control agent employed for the soybean pest, Anticarsia gemmatalis. This baculovirus biopesticide is currently produced using caterpillars, but increasing market demand for the product has encouraged the development of an in vitro manufacturing process, which can be scaled up to much higher virus productivities. In this study, three wild-type AgMNPV isolates (AgMNPV-2D, AgMNPV-MP2 and AgMNPV-MP5) and a recombinant form (vAgEGT-LacZ) were characterised in terms of occlusion body (OB) production and infection kinetics, to enable future optimisation of the in vitro production process. These viruses were propagated using a Spodoptera frugiperda (IPLB-SF21) insect cell line grown in shaker-flask batch cultures. Among the virus isolates tested, AgMNPV-MP5 was found to be the best producer, yielding (5.3±0.85)x108 OB/mL after 8 days post infection. The characterisation of vAgEGT-LacZ propagation in suspension cell cultures has not been previously reported in the literature; hence it became the main focus for this thesis. In particular, it was carried out a study on the effect of the multiplicity of infection (MOI) on OB production. Five successive batches were performed getting a final production (8.9±1.42)x1014 occlusion bodies, considering that production is related for a bioreactor with final volume of 10m3. A low MOI associated with a fed-batch process for vAgEGT-LacZ production was found to support a 3-fold higher OB yield when compared to the default batch process (1.8x107 and 5.3x107 OB/mL, respectively). This yield is competitive with regards to the production process.

Assessment of cytokine values in serum by RT-PCR in HIV-1 infected individuals with and without highly active anti-retroviral therapy (HAART)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Estudo geoquímico e geocronológico Rb-Sr e Sm-Nd em zonas de cisalhamento mineralizadas em ouro e suas relações com as rochas encaixantes e geocronológico Sm-Nd em mineralizações de scheelita na faixa Seridó

Great part of the gold mineralizations are associated with shearing zones through which circulate a great volume of fluids, that interact with the host rocks, originating leaching or precipitation of chemical elements, including gold. The studied mineralizations are inserted in the Seridó Belt. The tungsten mineralization in Brejuí Mine is hosted in calcsilicate rocks from Jucurutu Formation. The São Francisco auriferous mineralization has as host rocks mica-schists from Seridó Formation, while the Ponta da Serra and Fazenda Simpático mineralizations are hosted in orthogneisses of this fold belt basement. The research conducted on these mineralizations had the purpose of integrate the data of chemical elements behavior during the shearing/mineralizing event, and its influence on the isotopic systems Rb-Sr and Sm-Nd. The studies of chemical mobility in the auriferous mineralizations showed that elements that during the shearing displayed in general an immobile behavior were Al, Ti and Zr. Among the elements that were mobilized during the event, K and Rb showed mass gain in ali belts of transformed rocks, while the elements Ca, Na and Sr normally lost mass. Petrographic studies showed that the minerais biotite and plagioclase, in all investigated mineralizations, played an important role in the chemical reactions occurred in the transformed rocks to the generation of muscovite, cordierite and sillimanite, justifying the input of K to the formation of muscovite, and the release of Na and Ca from plagioclase to the fluid phase. In the São Francisco auriferous mineralization, the results of the Rb-Sr isotopic analysis yielded ages of 645 ± 19 Ma and 596 ± 17 Ma, with both samples, from original and transformed rocks. Two ages, 569 ± 20 Ma. and 554 ± 19 Ma., were obtained with samples frem the transformed rocks domain. These ages suggest that there were two metamorphic pulses during the emplacement of the mineralized shearing zone. The Sm-Nd data yielded TDM ages of 1,31 Ga and 1,26 Ga with 3Nd (0,6 Ga) of -0,26 e -0,40 for the original and final transformed rocks, respectively. In case of the orthogneisses of Caicó Complex, e.g. the Ponta da Serra and Fazenda Simpático mineralizations, the Rb-Sr data did not yield ages with geological significance. In the Ponta da Serra mineralization, the Sm-Nd isotopic data yielded T DM ages of 2,56 Ga and 2,63 Ga to the original rocks and of 2,71 Ga to the mineralized sheared rock, and values of 3Nd (2,0 Ga) between -3,70 e -5,42 to the original and sheared rock, respectively. In the Fazenda Simpático, Sm-Nd data yielded TDM between 2,65 and 2,69 Ga with values of 3Nd (2,0 Ga) between -5,25 e -5,52. Considering the Sm-Nd data, the TDM ages may be admitted as the age of the parental magma extraction, producer of the protoliths of the orthogneisses from Ponta da Serra and Fazenda Simpático mineralizations. The chemical mobility studies showed that in the basement hosted mineralizations, Rb achieved mass while Sr lost mass, as Sm as well as Nd were strongly mobilized. The Sm/Nd ratio remained constant, however, confirming the isochemical character of those elements. In the basement mineralizations, Rb-Sr ages are destituted of geological significance, because of the partial opening of the isotopic system during the tectono-metamorphic transformations. In the tungsten mineralization, the diagram Sm-Nd constructed with the whole-rock data of calcsilicatic and the high-temperature paragenesis (garnet, diopside and iron-pargasitic hornblende) indicated an 631 ± 24 Ma age, while with the whole-rock data and low-temperature paragenesis (vesuvianite, epidote and calcite), a 537 ± 107 Ma age was obtained. These ages, associated with the petrographic observations, suggest that there was a time gap among the hydrothernal events responsible by the formation of the high and low temperature paragenesis in the calcsilicatic rocks mineralized in scheelite

O processo de urbanização turística em Natal: a perspectiva do residente

The objective of the study was to analyze and discuss the process of urbanization in the tourist city of Natal focusing on the actions of the resident agent as a producer of space tourism. The production and expertise in the areas of the city for the performance of tourism have led to the intensification of social problems of nature that comes to few expelling the residents of the beaches. The cut space of the study included the four beaches of Natal / RN, the praias do Centro, Via Costeira, Ponta Negra beach in the Redinha. For this, was conducted a case study, using the technique of questioning, with the application of one hundred and sixteen (116) questionnaires to residents, with age from thirty-five years, between the four of the City Administrative Regions. The results show that the process of urbanization has interferido tourism on the practices of the recreation of residents in the areas as tourism has shown a trend seen in the intensifying problems of social order, such as prostitution, insecurity, pollution of beaches, etc. The combination of these problems are reflected negatively on the tourist areas and are gradually removing the residents. Through this framework problematic, given to tourist areas in the city, the resident still has not positioned the way criticizes the defense of these, choosing not express or adopting a form of discrete event, such as the gradual exit of the areas and electing tourist beaches in other municipalities. The results are of great significance for the direction of a trip planning committed with the participation of the resident in the planning process. The study is to corroborate with the importance of the participation of the resident, before the trip planning, as it is a fundamental agent for the sustainability of space tourism

A reprodução do espaço da pequena produção rural familiar em Canguaretama/RN

This study aimed to explore the process of reproduction of space from the small family farm production in the municipality of Canguaretama, specifically focused on foodstuffs of plant origin, seeking to understand the changes in agrarian space canguaretamense and its impact on small family farms the last 35 years. Since colonization, during the seventeenth century, the production of space agrarian Canguaretama was founded under a structure based on large ownership and cultivation of cane sugar. Secondly, it was being built a small space reserved for food production to meet both the consumption of property, but also for local marketing. In the centuries following the changes in the capitalist system imposed a new dynamic for small food production, mostly in the early twentieth century, with processing plants and mills in the area extending toward the cultivation of sugarcane. In the second half of that century, mainly in the 1980s, the cultivation of cane sugar was encouraged to produce alcohol, which led to a further expansion of sugar cane toward the areas targeted for the production of foodstuffs. Currently, the framework of small food production differs little from the period of colonization in relation to the difficulties faced by this segment of agriculture. Thus, we have a reality based on socio-spatial inequality, and the near absence of the Government, which requires urgent implementation of public policies for the production and organization of small producers into associations or cooperatives to improve the productivity and hence in their standards of living and their families

Aspectos microbiológicos e de qualidade do leite bovino

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Avaliação da produção de leite e contagem de células somáticas em bovinos leiteiros suplementads com Saccharomyces cerevisiae como fonte de zinco orgânico

The aim of the evaluation of milk production and somatic cell count of dairy cow supplemented with Saccharomyces cerevisiae as a source of organic zinc for 180 days, 25 Holstein cows were selected, randomly chosen from a flock of 189 lactating cows. The animals were distributed in two groups, namely group 1 (G1) which holded 10 cows supplemented and group 2 (G2) with 15 animals without supplementation. The production of milk was measured by the control official milkman of the Assocition Paranaense of Creators of Bovine of the Holstein in seven moments during the 180 days. The samples of milk were collected of each animal, being submitted to the electronic counting of somatic cells. The results demonstrate that the supplemented of organic zinc didn't alter the production of milk, however it was capable to maintain low the counting of somatic cells. The data of the present work suggest that to use supplemented of organic zinc in the diet of cows milk, increase the quality of the produced milk and consequently the remuneration for the producer.

Desempenho zootécnico e percentual de consumo de alimento de rã-touro (Rana catesbeiana) na fase de recria (pós-metamorfose) do sistema anfigranja

Objetivou-se com este trabalho avaliar (em nível de campo) o desempenho zootécnico da rã-touro, Rana catesbeiana (Shaw, 1882), criada em ranários comerciais que utilizam o Sistema Anfigranja, e , simultaneamente, estimar os valores de consumo diário de alimento para compor uma tabela de referência para o arraçoamento dos animais. As avaliações demonstraram haver valores de produtividade com grandes variações, de acordo com a capacidade do produtor em assimilar a tecnologia e principalmente manejar os animais de forma padronizada, para atingir o ponto de equilíbrio no fluxo de produção de seu plantel. Os resultados encontrados nas unidades de observação monitoradas (aproximadamente 250 mil animais) atingiram os seguintes índices médios: a mortalidade (em %) 0,5 a 39,5 (média geral 12,0); o ganho de peso (g./dia) 0,4 a 2,5 (média geral 1,2); e a conversão alimentar 0,9 a 2,5 : 1,0 (média geral 1,4 : 1,0). É apresentada uma tabela com os percentuais estimados de consumo de alimento, em função do peso médio dos animais, para o cálculo da quantidade de ração a ser oferecida diariamente.

Detection of turkey coronavirus in commercial turkey poults in Brazil

Poult enteritis complex has been incriminated as a major cause of loss among turkey poults in other countries. We have observed this in Brazil, associated with diarrhoea, loss of weight gain and, commonly, high mortality In this study, we have used the reverse transcriptase polymerase chain reaction (RT-PCR) to detect turkey coronavirus (TCoV) in sick poults 30 to 120 days of age from a particular producer region in Brazil. The RT-PCR was applied to extracts of intestine tissue suspensions, and the respective intestinal contents, bursa of Fabricius, faecal droppings and cloacal swabs. Primers were used to amplify the conserved 3' untranslated region of the genome, and the nucleocapsid protein gene of TCoV Histo pathological and direct immunohistochemical examinations were performed to detect TCoV antigen in infected intestine and bursa slides. All the results from stained tissues revealed lesions as described previously for TCoV infection. The direct immunohistochemical positive signal was present in all intestine slides. However, all bursa of Fabricius tissues analysed were negative. RT-PCR findings were positive for TCoV in all faecal droppings samples, and in 27% of cloacal swabs. Finally, the best field material for TCoV diagnosis was faecal droppings and/or intestine suspensions.

Multiobjective 0-1 integer programming for the use of sugarcane residual biomass in energy cogeneration

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)