Turystyka i rekreacja a jakość życia mieszkańców Leszna

The quality of life (QOL) is currently one of the main fields of study for researchers around the world. Its complex nature makes it a topic of interest for many different fields, such as philosophy, ethics, medicine, economics, sociology and physical culture sciences. Despite the differences in the way various researches define the QOL, the general agreement is that any measurement of QOL should encompass the following dimensions: objective QOL and subjective QOL (well-being). Particularly noteworthy, for any complex research is the mutual relationship between the QOL and tourism and recreation. The increase of tourism and recreation should entail a simultaneous development of local communities, as emphasized by Crouch and Ritchie this implies that the inhabitants of an area that serves a function of tourism and recreation should profit from the increased number of tourists. This benefits should be reflected in the objective QOL conditions and the subjective sense of satisfaction of the residents. The city of Leszno and it’s tourism and recreation-oriented development strategy were used to form a basis for theoretical study on QOL and to calculate the Tourism-Recreation QOL Index.

Metabolite profiling of somatic embryos of Cyclamen persicum in comparison to zygotic embryos, endosperm, and testa

Somatic embryogenesis has been shown to be an efficient in vitro plant regeneration system for many crops such as the important ornamental plant Cyclamen persicum, for which this regeneration pathway of somatic embryogenesis is of interest for the vegetative propagation of parental lines as well as elite plants. However, somatic embryogenesis is not commercially used in many crops due to several unsolved problems, such as malformations, asynchronous development, deficiencies in maturation and germination of somatic embryos. In contrast, zygotic embryos in seeds develop and germinate without abnormalities in most cases. Instead of time-consuming and labor-intensive experiments involving tests of different in vitro culture conditions and plant growth regulator supplements, we follow a more directed approach. Zygotic embryos served as a reference and were compared to somatic embryos in metabolomic analyses allowing the future optimization of the in vitro system. The aims of this study were to detect differences in the metabolite profiles of torpedo stage somatic and zygotic embryos of C. persicum. Moreover, major metabolites in endosperm and testa were identified and quantified. Two sets of extracts of two to four biological replicates each were analyzed. In total 52 metabolites were identified and quantified in the different tissues. One of the most significant differences between somatic and zygotic embryos was that the proline concentration in the zygotic embryos was about 40 times higher than that found in somatic embryos. Epicatechin, a scavenger for reactive oxygen species, was found in highest abundance in the testa. Sucrose, the most abundant metabolite was detected in significantly higher concentrations in zygotic embryos. Also, a yet unknown trisaccharide, was significantly enriched in zygotic embryos.

Evaluation of the cytotoxic and genotoxic effects of benchmark multi-walled carbon nanotubes in relation to their physicochemical properties

To contribute with scientific evidence to the grouping strategy for the safety assessment of multi-walled carbon nanotubes (MWCNTs), this work describes the investigation of the cytotoxic and genotoxic effects of four benchmark MWCNTs in relation to their physicochemical characteristics, using two types of human respiratory cells. The cytotoxic effects were analysed using the clonogenic assay and replication index determination. A 48h-exposure of cells revealed that NM-401 was the only cytotoxic MWCNT in both cell lines, but after 8-days exposure, the clonogenic assay in A549 cells showed cytotoxic effects for all the tested MWCNTs. Correlation analysis suggested an association between the MWCNTs size in cell culture medium and cytotoxicity. No induction of DNA damage was observed after any MWCNTs in any cell line by the comet assay, while the micronucleus assay revealed that both NM-401 and NM-402 were genotoxic in A549 cells. NM-401 and NM-402 are the two longest MWCNTs analyzed in this work, suggesting that length may be determinant for genotoxicity. No induction of micronuclei was observed in Beas-2B cell line and the different effect in both cell lines is explained in view of the size-distribution of MWCNTs in the cell culture medium, rather than cell's specificities.

Iron Transformation Pathways and Redox Micro-Environments in Seafloor Sulfide-Mineral Deposits: Spatially Resolved Fe XAS and delta Fe-57/54 Observations

Hydrothermal sulfide chimneys located along the global system of oceanic spreading centers are habitats for microbial life during active venting. Hydrothermally extinct, or inactive, sulfide deposits also host microbial communities at globally distributed sites. The main goal of this study is to describe Fe transformation pathways, through precipitation and oxidation-reduction (redox) reactions, and examine transformation products for signatures of biological activity using Fe mineralogy and stable isotope approaches. The study includes active and inactive sulfides from the East Pacific Rise 9 degrees 50'N vent field. First, the mineralogy of Fe(III)-bearing precipitates is investigated using microprobe X-ray absorption spectroscopy (RXAS) and X-ray diffraction (mu XRD). Second, laser-ablation (LA) and micro-drilling (MD) are used to obtain spatially-resolved Fe stable isotope analysis by multicollector-inductively coupled plasma-mass spectrometry (MC-ICP-MS). Eight Fe -bearing minerals representing three mineralogical classes are present in the samples: oxyhydroxides, secondary phyllosilicates, and sulfides. For Fe oxyhydroxides within chimney walls and layers of Si-rich material, enrichments in both heavy and light Fe isotopes relative to pyrite are observed, yielding a range of delta Fe-57 values up to 6 parts per thousand. Overall, several pathways for Fe transformation are observed. Pathway 1 is characterized by precipitation of primary sulfide minerals from Fe(II)aq-rich fluids in zones of mixing between vent fluids and seawater. Pathway 2 is also consistent with zones of mixing but involves precipitation of sulfide minerals from Fe(II)aq generated by Fe(III) reduction. Pathway 3 is direct oxidation of Fe(II) aq from hydrothermal fluids to form Fe(III) precipitates. Finally, Pathway 4 involves oxidative alteration of pre-existing sulfide minerals to form Fe(III). The Fe mineralogy and isotope data do not support or refute a unique biological role in sulfide alteration. The findings reveal a dynamic range of Fe transformation pathways consistent with a continuum of micro-environments having variable redox conditions. These micro-environments likely support redox cycling of Fe and S and are consistent with culture-dependent and -independent assessments of microbial physiology and genetic diversity of hydrothermal sulfide deposits.

Towards an understanding of Pakistani undergraduates’ current attitudes towards learning and speaking English

The English language has an important place in Pakistan and in its education system, not least because of the global status of English and its role in employment. Realising the need to enhance language learning outcomes, especially at the tertiary level, the Higher Education Commission (HEC) of Pakistan has put in place some important measures to improve the quality of English language teaching practice through its English Language Teaching Reforms (ELTR) project. However, there is a complex linguistic, educational and ethnic diversity in Pakistan and that diversity, alongside the historical and current role of English in the country, makes any language teaching reform particularly challenging. I argue, in this thesis, that reform to date has largely ignored the issues of learner readiness to learn and learner perceptions of the use of English. I argue that studying learner attitudes is important if we are to understand how learners perceive the practice of learning and the use of English in their lives. This study focuses on the attitudes of undergraduate learners of English as a foreign language at two universities in the provinces of Sindh and Balochistan in Pakistan. These provinces have experienced long struggles and movements related to linguistic and ethnic rights and both educate students from all of the districts of their respective provinces. Drawing on debates around linguistic imperialism, economic necessity, and linguistic and educational diversity, I focus on learners’ perceptions about learning and speaking English, asking what their attitudes are towards learning and speaking English with particular reference to socio-psychological factors at a given time and context, including perceived threats to their culture, religion, and mother tongue. I ask how they make choices about learning and speaking English in different domains of language use and question their motivation to learn and speak English. Additionally, I explore issues of anxiety with reference to their use of English. Following a predominantly qualitative mixed methods research approach, the study employs two research tools: an adapted Likert Scale questionnaire completed by 300 students and semi-structured interviews with 20 participants from the two universities. The data were analysed through descriptive statistics and qualitative content analysis, with each set of data synthesised for interpretation. The findings suggest that, compared with the past, the majority of participants hold positive attitudes towards learning and speaking English regardless of their ethnic or linguistic backgrounds. Most of these undergraduate students do not perceive the use of English as a threat to their culture, mother tongue or religious values but, instead, they have a pragmatic and, at the same time, aspirational attitude to the learning and use of English. I present these results and conclude this thesis with reference to ways in which this small-scale study contributes to a better understanding of learner attitudes and perceptions. Acknowledging the limitations of this study, I suggest ways in which the study, enhanced and extended by further research, might have implications for practice, theory and policy in English language teaching and learning in Pakistan.

Starch analysis as a method to discover subsistence strategies in prehistoric Finland and Estonia

Ancient starch analysis is a microbotanical method in which starch granules are extracted from archaeological residues and the botanical source is identified. The method is an important addition to established palaeoethnobotanical research, as it can reveal ancient microremains of starchy staples such as cereal grains and seeds. In addition, starch analysis can detect starch originating from underground storage organs, which are rarely discovered using other methods. Because starch is tolerant of acidic soils, unlike most organic matter, starch analysis can be successful in northern boreal regions. Starch analysis has potential in the study of cultivation, plant domestication, wild plant usage and tool function, as well as in locating activity areas at sites and discovering human impact on the environment. The aim of this study was to experiment with the starch analysis method in Finnish and Estonian archaeology by building a starch reference collection from cultivated and native plant species, by developing sampling, measuring and analysis protocols, by extracting starch residues from archaeological artefacts and soils, and by identifying their origin. The purpose of this experiment was to evaluate the suitability of the method for the study of subsistence strategies in prehistoric Finland and Estonia. A total of 64 archaeological samples were analysed from four Late Neolithic sites in Finland and Estonia, with radiocarbon dates ranging between 2904 calBC and 1770 calBC. The samples yielded starch granules, which were compared with the starch reference collection and descriptions in the literature. Cereal-type starch was identified from the Finnish Kiukainen culture site and from the Estonian Corded Ware site. The samples from the Finnish Corded Ware site yielded underground storage organ starch, which may be the first evidence of the use of rhizomes as food in Finland. No cereal-type starch was observed. Although the sample sets were limited, the experiment confirmed that starch granules have been preserved well in the archaeological material of Finland and Estonia, and that differences between subsistence patterns, as well as evidence of cultivation and wild plant gathering, can be discovered using starch analysis. By collecting large sample sets and addressing the three most important issues – preventing contamination, collecting adequate references and understanding taphonomic processes – starch analysis can substantially contribute to research on ancient subsistence in Finland and Estonia.

Sistema de gestión y guía turística para dispositivos móviles en El Salvador

Este documento, refiere al trabajo realizado para la Alcaldía Municipal de San Vicente, con el propósito de ayudar a la promoción del turismo en el Municipio de San Vicente y en toda la Zona paracentral, se desarrolló una aplicación web que se compone de tres módulos principales a los cuales se puede acceder desde un dispositivo móvil con acceso a internet y un módulo para administradores del sistema para la gestión de centros turísticos y turistas los cuales serán operados desde un administrador principal, el sistema se desarrolló para poder contribuir a la promoción del turismo en El Salvador. Esta herramienta se desarrolló usando las tecnologías existentes para dispositivos móviles de HTML5 y PHP. Al finalizar el aplicativo web se han logrado obtener beneficios para los dueños de destinos turísticos ya que pueden promocionarse de manera gratuita, además la Institución beneficiaria cuenta con un recurso más para poder catalogar y mapear destinos existentes y así sugerirlos. Al finalizar este trabajo se logró cumplir con el objetivo planteado que fue ayudar a la promoción del turismo en El Salvador. SUMMARY This document refers to work done for the Municipality of San Vicente, in order to help promote tourism in the municipality of San Vicente and throughout the paracentral area, is developing a web application that consists of three main modules to which can be accessed from a mobile device with internet access and a module for system administrators to manage resorts and tourists which will be operated from a main administrator, the system development to contribute to the promotion of tourism in El Salvador. This tool was developed using existing technologies for mobile devices HTML5 and PHP. At the end of the web application has been made a profit for the owners of tourist destinations because they can promote themselves for free, also the beneficiary institution has a resource to catalog and map existing and destinations and suggest them. Upon completion of this work failed to meet the stated objective was to help the promotion of tourism in El Salvador.

O city marketing em Natal/RN e a construção da imagem da cidade

The growing importance of tourism in overall economic activity worldwide has favored the intensification of competition among cities that seek to create environments attractive to tourists and potential investors. It has been common practice to import characteristics of the business environment in the public management of cities. The city marketing is a key tool used by public leaders to promote a linkage between the tourism image and urban image and involves, in addition to promoting the image of the city, the planning of interventions in urban space, trying to formulate a positive image of the city able to facilitate the deployment of capital. This research seeks to understand the nature of city marketing as part of contemporary urban management and analyzes how is its application in decisions concerning the promotion of tourism in Natal/RN. The approach of this research is qualitative, exploratory and descriptive, in which respondents were the main leaders of two of the official tourism site, the Empresa Potiguar de Promoção Turística and the Secretaria Municipal de Turismo e Desenvolvimento Econômico. It was found that there is a strong articulation of public power with private enterprise in the design and conduct of the actions of urban marketing, that from the survey data show that the behavior of target markets provide guidelines for taking strategic decisions relating to tourism. Sun and sea are some key elements explored to form the image of Natal and to authorize the sale of the city as a tropical paradise. However, there is an increase in the diversification of tourism products, seeking to increase flow to the segments of ecotourism, adventure, business and culture. It s also growing the use of local culture as a tourism product, however, the cultural representation focuses on superficial values and does not bring to light the social and historical richness that the city has. Public authorities use the city marketing strategies as a means able to maximize the attractiveness of Natal urban space to investors, business groups and tourists. It can be observed that urban managers seek solutions that can continuously increase the tours, which often manifests in interventions that focus the tourist areas of the city, in oposition of those who do not contribute to a positive reading of the city, which ultimately generate the worsening of spacial and social inequalities

Antimicrobial Compounds from Eukaryotic Microalgae against Human Pathogens and Diseases in Aquaculture

The search for novel compounds of marine origin has increased in the last decades for their application in various areas such as pharmaceutical, human or animal nutrition, cosmetics or bioenergy. In this context of blue technology development, microalgae are of particular interest due to their immense biodiversity and their relatively simple growth needs. In this review, we discuss about the promising use of microalgae and microalgal compounds as sources of natural antibiotics against human pathogens but also about their potential to limit microbial infections in aquaculture. An alternative to conventional antibiotics is needed as the microbial resistance to these drugs is increasing in humans and animals. Furthermore, using natural antibiotics for livestock could meet the consumer demand to avoid chemicals in food, would support a sustainable aquaculture and present the advantage of being environmentally friendly. Using natural and renewable microalgal compounds is still in its early days, but considering the important research development and rapid improvement in culture, extraction and purification processes, the valorization of microalgae will surely extend in the future.

Visit Cabo Verde

A indústria turística tem-se focado na diversificação, de forma contínua, tornando-se um dos motores fundamentais para o progresso dos setores económicos de um país. Para tal, o desenvolvimento de um Produto Turístico de sucesso que visa oferecer ao público-alvo uma experiência memorável para satisfazer o seu desejo é essencial para que este consumidor venha a visitar um determinado destino. A nova era digital transformou a estratégia de marketing das empresas e esta nova conjuntura tem vindo a contribuir para o crescimento exponencial e para o desenvolvimento das técnicas e ferramentas que permitem promover a comunicação entre a empresa/marca e o consumidor, aspeto em que o marketing digital tem desempenhado um papel importante. Em tempos de valorização da experiência turística, o setor turístico de Cabo Verde pouco tem explorado e não tem tirado grande partido do marketing digital para dar a conhecer as potencialidades e as maravilhas do destino enquanto produto turístico, que ainda tem muito por descobrir por parte dos consumidores nacionais e internacionais. Como objetivos deste plano de comunicação Visit Cabo Verde pretende-se aumentar a notoriedade da marca Cabo Verde e captar novos turistas através da promoção de 4 ilhas: Santiago, São Vicente, Santo Antão e Boavista dividindo-as em 3 (três) subprodutos - cultura, natureza e praia. Com a realização deste projeto prevê-se que Cabo Verde atinja o público-alvo definido através da conceção das campanhas relevantes para os potenciais turistas e aumentar o fluxo dos turistas no arquipélago. As limitações deste plano de comunicação incidem sobre a variável produto que não é controlável, procurando este plano uma linha de orientação para a variável comunicação.

Antifungal activity of extracts from Atacama Desert fungi against Paracoccidioides brasiliensis and identification of Aspergillus felis as a promising source of natural bioactive compounds

Fungi of the genus Paracoccidioides are responsible for paracoccidioidomycosis. The occurrence of drug toxicity and relapse in this disease justify the development of new antifungal agents. Compounds extracted from fungal extract have showing antifungal activity. Extracts of 78 fungi isolated from rocks of the Atacama Desert were tested in a microdilution assay against Paracoccidioides brasiliensis Pb18. Approximately 18% (5) of the extracts showed minimum inhibitory concentration (MIC) values ≤ 125.0 μg/mL. Among these, extract from the fungus UFMGCB 8030 demonstrated the best results, with an MIC of 15.6 μg/mL. This isolate was identified as Aspergillus felis (by macro and micromorphologies, and internal transcribed spacer, β-tubulin, and ribosomal polymerase II gene analyses) and was grown in five different culture media and extracted with various solvents to optimise its antifungal activity. Potato dextrose agar culture and dichloromethane extraction resulted in an MIC of 1.9 μg/mL against P. brasiliensis and did not show cytotoxicity at the concentrations tested in normal mammalian cell (Vero). This extract was subjected to bioassay-guided fractionation using analytical C18RP-high-performance liquid chromatography (HPLC) and an antifungal assay using P. brasiliensis. Analysis of the active fractions by HPLC-high resolution mass spectrometry allowed us to identify the antifungal agents present in the A. felis extracts cytochalasins. These results reveal the potential of A. felis as a producer of bioactive compounds with antifungal activity.

Early consumption of liquids different to breast milk in Mexican infants under 1 year: results of the probabilistic National Health and Nutrition Survey 2012

Introduction: Studies on infant dietary intake do not generally focus on the types of liquids consumed. Objective: To document by age and breastfeeding status, the types of liquids present in the diet of Mexican children under 1 year of age (< 1 y) who participated in the National Health and Nutrition Survey 2012 (ENSANUT-2012). Methods: Analysis of the infant < 1 y feeding practices from the ENSANUT-2012 survey in non-breastfed (non-BF) and breastfed (BF) infants by status quo for the consumption of liquids grouped in: water, formula, fortified LICONSA milk, nutritive liquids (NL; thin cereal-based gruel with water or milk and coffee with milk) and non-nutritive liquids (non-NL) as sugared water, water-based drinks, tea, beans or chicken broth, aguamiel and coffee. In this infants < 1 y we analyzed the not grouped consumption of liquids in the first three days of life (newborns) from the mother's recall. Percentage and confidence intervals (95% CI) were calculated adjusting for survey design. Statistical differences were analyzed by Z test. Results: We observed a high consumption of human milk followed by formula (56.7%) and water (51.1%) in infants under 6 months of age (< 6 mo). The proportion of non-BF infants consuming non-NL was higher than for BF infants (p < 0.05). More than 60% of older infants (6 mo and < 1 y) consumed formula and were non-BF. In newborns formula consumption was predominant, followed by tea or infusion and water. Conclusions: Non-breast milk liquids are present undesirably in Mexican infants' diet and non-NL are consumed earlier than NL, revealing inadequate early dietary practices.

Eliciting nature and landscape writing through outdoor experiences

Nature and landscape writing includes creative writing about wild places. However, most authors have a literary background and are not outdoor ‘educators’. Using a hermeneutic phenomenological approach, the reasons suggested are a lack of framing of outdoor experiences for this intent, the need for learning the skills of interpretation and lexicon and the offer of prolonged, powerful experiences and time for creative thinking and responses, such as an extended solo. It is suggested that outdoor educators may be too busy ‘experiencing’ to write, that they do not go ‘slow’ enough or that they are encapsulated in the ‘edginess of existence’ through adventure and just pass through their surroundings rather than connect with them. Outdoor educators have much to offer as they experience metaphorical or literal journeys comprising ‘flow’ rather than episodic encounter through lived experience to create rich embodied stories with ideological and social aspects so often overlooked in narrative.

Análisis, dignóstico y propuesta para fomentar el destino turístico Bolivia enfocado al turismo emisivo de la ciudad de Cuenca, con la ruta que efrece LAN hacia La Paz y Santa Cruz 2015-2016

El presente trabajo de investigación tiene como objetivo conocer la oferta y demanda del mercado turístico emisivo hacia Bolivia desde la ciudad de Cuenca, con la finalidad de fomentar el turismo hacia nuevos destinos, enfocado principalmente en las ciudades de: La Paz y Santa Cruz de la Sierra, con la ruta que ofrece la aerolínea LAN hacia estos destinos. Para el desarrollo de la tesis se investigó el comportamiento de los pasajeros que deciden comprar los boletos en las oficinas de LAN, y las particularidades de las agencias de viajes que tienen convenio con la mencionada aerolínea, con la finalidad de determinar si existe un potencial mercado emisivo en la ciudad de Cuenca que esté dispuesto a visitar Bolivia; los resultados que se obtuvieron en este análisis definenprincipalmente la oferta y la demanda actual; adicionalmente se obtuvo la información necesaria de las agencias de viajes para informar y capacitar a los agentes de viajes, sobre los diferentes atractivos turísticos y sitios de interés con los que cuenta Bolivia; en base de toda ésta información, se propone la creación de diferentes paquetes con alternativas de turismo para los pasajeros en la ciudad de Cuenca. El aporte que se suministra con el desarrollo de ésta investigación, principalmente a las agencias de viajes y promotores turísticos es el de demostrar que se pueden ofertar lugares nuevos, que aún no han sido explotados en el mercado emisivo de la ciudad de Cuenca, para que no se limiten únicamente a competir por los productos de siempre previamente estructurados y saturados.

Desporto e turismo : caraterização dos serviços desportivos e perceção da procura turística em unidades de alojamento do Algarve

O turismo desportivo é um fenómeno que tem sido estudado a partir da segunda metade do século XX, começando a existir uma oferta mais diversificada e planeada de férias em conjunto com a prática desportiva que, segundo Pereira & Carvalho (2004), favoreceu a consolidação do conceito Desporto e Turismo. Segundo Higham (2005), os destinos turísticos são lugares que atraem e satisfazem as necessidades dos visitantes, nomeadamente ao nível: dos recursos, das infra-estruturas e dos serviços necessários para facilitar as viagens para o destino, e da realização de experiências satisfatórias. Neste âmbito, os objetivos deste estudo são identificar a perceção dos gestores quanto à procura desportiva e analisar a oferta desportiva nas unidades de alojamento de 4 e 5 estrelas da região do Algarve. Para este estudo foi feita uma recolha de dados em 43 unidades de alojamento, através de um inquérito por questionário e utilizado como método de análise de dados a estatística descritiva para tratamento de questões fechadas e a análise de conteúdo nas questões abertas. Os resultados mostram que o perfil do turista do Algarve mudou, revelando que os turistas de nacionalidade inglesa pertencentes ao segmento de famílias se apresenta valores mais elevados de procura na época alta, enquanto que na época baixa o segmento de turismo sénior é o que surge em primeiro lugar da procura, sendo o tipo de turismo de sol e mar mais procurado em ambas as épocas. Conclui-se que não existem valores elevados de procura por parte de turistas cuja principal motivação seja a prática desportiva. Verificou-se ainda que tem havido uma evolução ao nível dos serviços desportivos com a existência de mais instalações e responsáveis de seção desportiva nas unidades de alojamento. Os gestores afirmam ter uma oferta adequada em matéria de desporto e que nos próximos 6 meses não irão investir. Relativamente à frequência de utilização das instalações é muito baixa. Ser pago ou gratuito também é um fator influenciador para haver uma maior frequência de utilização.