Antifungal activity of extracts from Atacama Desert fungi against Paracoccidioides brasiliensis and identification of Aspergillus felis as a promising source of natural bioactive compounds


Autoria(s): Mendes, Graziele; Gonçalves, Vívian N.; Souza-Fagundes, Elaine M.; Kohlhoff, Markus; Rosa, Carlos A.; Zani, Carlos L.; Cota, Betania B.; Rosa, Luiz H.; Johann, Susana
Cobertura

Origin of publication: Brazil

Data(s)

06/07/2016

Resumo

Fungi of the genus Paracoccidioides are responsible for paracoccidioidomycosis. The occurrence of drug toxicity and relapse in this disease justify the development of new antifungal agents. Compounds extracted from fungal extract have showing antifungal activity. Extracts of 78 fungi isolated from rocks of the Atacama Desert were tested in a microdilution assay against Paracoccidioides brasiliensis Pb18. Approximately 18% (5) of the extracts showed minimum inhibitory concentration (MIC) values ≤ 125.0 μg/mL. Among these, extract from the fungus UFMGCB 8030 demonstrated the best results, with an MIC of 15.6 μg/mL. This isolate was identified as Aspergillus felis (by macro and micromorphologies, and internal transcribed spacer, β-tubulin, and ribosomal polymerase II gene analyses) and was grown in five different culture media and extracted with various solvents to optimise its antifungal activity. Potato dextrose agar culture and dichloromethane extraction resulted in an MIC of 1.9 μg/mL against P. brasiliensis and did not show cytotoxicity at the concentrations tested in normal mammalian cell (Vero). This extract was subjected to bioassay-guided fractionation using analytical C18RP-high-performance liquid chromatography (HPLC) and an antifungal assay using P. brasiliensis. Analysis of the active fractions by HPLC-high resolution mass spectrometry allowed us to identify the antifungal agents present in the A. felis extracts cytochalasins. These results reveal the potential of A. felis as a producer of bioactive compounds with antifungal activity.

Formato

html

Identificador

http://www.bioline.org.br/abstract?id=oc16029

Idioma(s)

en

Publicador

Fundação Oswaldo Cruz, Fiocruz

Relação

http://memorias.ioc.fiocruz.br; http://www.bioline.org.br/oc

Direitos

Copyright 2016 - Memórias do Instituto Oswaldo Cruz

Fonte

Memórias do Instituto Oswaldo Cruz (ISSN: 1678-8060) Vol 111 Num 3

Palavras-Chave #rock-inhabiting fungi; Atacama Desert; Paracoccidioides brasiliensis; antifungal; Aspergillus felis
Tipo

AA