The use of unmanned aerial vehicles and wireless sensor networks for spraying pesticides

The application of pesticides and fertilizers in agricultural areas is of crucial importance for crop yields. The use of aircrafts is becoming increasingly common in carrying out this task mainly because of their speed and effectiveness in the spraying operation. However, some factors may reduce the yield, or even cause damage (e.g., crop areas not covered in the spraying process, overlapping spraying of crop areas, applying pesticides on the outer edge of the crop). Weather conditions, such as the intensity and direction of the wind while spraying, add further complexity to the problem of maintaining control. In this paper, we describe an architecture to address the problem of self-adjustment of the UAV routes when spraying chemicals in a crop field. We propose and evaluate an algorithm to adjust the UAV route to changes in wind intensity and direction. The algorithm to adapt the path runs in the UAV and its input is the feedback obtained from the wireless sensor network (WSN) deployed in the crop field. Moreover, we evaluate the impact of the number of communication messages between the UAV and the WSN. The results show that the use of the feedback information from the sensors to make adjustments to the routes could significantly reduce the waste of pesticides and fertilizers.

Hepatitis C: a changing epidemic.

Approximately 3% of the world population is estimated to have a chronic hepatitis C virus (HCV) infection and 500,000 individuals die from its consequences yearly. Persons who inject drugs (PWID) bear the majority of the disease burden in high-income countries. Drug substitution programmes have helped reduce HCV transmissions among PWID. However, recent epidemics of sexually transmitted HCV infections in HIV-infected men who have sex with men demonstrated the changing nature of the HCV epidemic. HCV therapy is undergoing a revolution, as new interferon-free, oral treatments eradicate HCV infections in almost all treated patients. As a consequence, the eradication of HCV has become a matter of debate and is becoming an important future public health target. However, for this to be achieved, many challenges need to be addressed, including the poor uptake of HCV testing, the high cost of the new antiviral combinations and the high frequency of re-infections after treatment in some populations.

HIV and human herpesvirus 8 co-infection across the globe: Systematic review and meta-analysis.

HIV-infection is an important risk factor for developing Kaposi sarcoma (KS), but it is unclear whether HIV-positive persons are also at increased risk of co-infection with human herpesvirus 8 (HHV-8), the infectious cause of KS. We systematically searched literature up to December 2012 and included studies reporting HHV-8 seroprevalence for HIV-positive and HIV-negative persons. We used random-effects meta-analysis to combine odds ratios (ORs) of the association between HIV and HHV-8 seropositivity and conducted random-effects meta-regression to identify sources of heterogeneity. We included 93 studies with 58,357 participants from 32 countries in sub-Saharan Africa, North and South America, Europe, Asia, and Australia. Overall, HIV-positive persons were more likely to be HHV-8 seropositive than HIV-negative persons (OR 1.99, 95% confidence interval [CI] 1.70-2.34) with considerable heterogeneity among studies (I(2) 84%). The association was strongest in men who have sex with men (MSM, OR 3.95, 95% CI 2.92-5.35), patients with hemophilia (OR 3.11, 95% CI 1.19-8.11), and children (OR 2.45, 95% CI 1.58-3.81), but weaker in heterosexuals who engage in low-risk (OR 1.42, 95% CI 1.16-1.74) or high-risk sexual behavior (OR 1.66, 95% CI 1.27-2.17), persons who inject drugs (OR 1.66, 95% CI 1.28-2.14), and pregnant women (OR 1.68, 95% CI 1.15-2.47), p value for interaction <0.001. In conclusion, HIV-infection was associated with an increased HHV-8 seroprevalence in all population groups examined. A better understanding of HHV-8 transmission in different age and behavioral groups is needed to develop strategies to prevent HHV-8 transmission.

Interactions of climate and land use documented in the varved sediments of Seebergsee in the Swiss Alps

This paper presents a multiproxy high-resolution study of the past 2600 years for Seebergsee, a small Swiss lake with varved sediments at the present tree-line ecotone. The laminae were identified as varves by a numerical analysis of diatom counts in the thin-sections. The hypothesis of two diatom blooms per year was corroborated by the 210Pb and 137Cs chronology. A period of intensive pasturing during the ‘Little Ice Age’ between ad 1346 and ad 1595 is suggested by coprophilous fungal spores, as well as by pollen indicators of grazing, by the diatom-inferred total phosphorus, by geochemistry and by documentary data. The subsequent re-oligotrophication of the lake took about 88 years, as determined by the timelag between the decline of coprophile fungal spores and the restoration of pre-eutrophic nutrient conditions. According to previous studies of latewood densities from the same region, cold summers around ad 1600 limited the pasturing at this altitude. This demonstrated the socio-economic impact of a single climatic event. However, the variance partitioning between the effects of land use and climate, which was applied for the whole core, revealed that climate independent of land use and time explained only 1.32% of the diatom data, while land use independent of climate and time explained 15.7%. Clearly land use in‘ uenced the lake, but land use was not always driven by climate. Other factors beside climate, such as politics or the introduction of fertilizers in the seventeenth and eighteenth centuries also in‘ uenced the development of Alpine pasturing.

Polydnavirus DNA of the braconid wasp Chelonus inanitus is integrated in the wasp's genome and excised only in later pupal and adult stages of the female

Many endoparasitic wasps inject, along with the egg, polydnavirus into their insect hosts, the virus being a prerequisite for successful parasitoid development. The genome of polydnaviruses consists of multiple circular dsDNA molecules of variable size. We show for a 12 kbp segment of the braconid Chelonus inanitus (CiV12) that it is integrated into the wasp genome. This is the first direct demonstration of integration for a bracovirus. PCR data indicated that the integrated form of CiV12 was present in all male and female stages investigated while the excised circular virus DNA only appeared in females after a specific stage in pupal-adult development. The data also indicated that after excision of virus DNA the genomic DNA was rejoined. This has not yet been reported for any polydnavirus. Sequence analyses in the junction regions revealed the presence of an imperfect consensus sequence of 15 nucleotides in CiV12, in each terminus of the integrated virus DNA and in the rejoined genomic DNA. Within these repeats two sequence types (ATA, TAC) were observed in the various virus clones and in the clones encompassing the rejoined genomic DNA; they corresponded to the sequence type in the right and left junction, respectively. To explain this, we propose a model of virus DNA replication in which the genomic DNA is folded to juxtapose the direct repeat of the left with that of the right junction; recombination at specific sites would then yield the two types of virus and rejoined genomic DNA.

A photopolymerized composite hydrogel and surgical implanting tool for a nucleus pulposus replacement

Nucleus pulposus replacements have been subjected to highly controversial discussions over the last 40 years. Their use has not yet resulted in a positive outcome to treat herniated disc or degenerated disc disease. The main reason is that not a single implant or tissue replacement was able to withstand the loads within an intervertebral disc. Here, we report on the development of a photo-polymerizable poly(ethylene glycol)dimethacrylate nano-fibrillated cellulose composite hydrogel which was tuned according to native tissue properties. Using a customized minimally-invasive medical device to inject and photopolymerize the hydrogel insitu, samples were implanted through an incision of 1 mm into an intervertebral disc of a bovine organ model to evaluate their long-term performance. When implanted into the bovine disc model, the composite hydrogel implant was able to significantly re-establish disc height after surgery (p < 0.0025). The height was maintained after 0.5 million loading cycles (p < 0.025). The mechanical resistance of the novel composite hydrogel material combined with the minimally invasive implantation procedure into a bovine disc resulted in a promising functional orthopedic implant for the replacement of the nucleus pulposus.

Late presentation for HIV care across Europe: update from the Collaboration of Observational HIV Epidemiological Research Europe (COHERE) study, 2010 to 2013.

Late presentation (LP) for HIV care across Europe remains a significant issue. We provide a cross-European update from 34 countries on the prevalence and risk factors of LP for 2010-2013. People aged ≥ 16 presenting for HIV care (earliest of HIV-diagnosis, first clinic visit or cohort enrollment) after 1 January 2010 with available CD4 count within six months of presentation were included. LP was defined as presentation with a CD4 count < 350/mm(3) or an AIDS defining event (at any CD4), in the six months following HIV diagnosis. Logistic regression investigated changes in LP over time. A total of 30,454 people were included. The median CD4 count at presentation was 368/mm(3) (interquartile range (IQR) 193-555/mm(3)), with no change over time (p = 0.70). In 2010, 4,775/10,766 (47.5%) were LP whereas in 2013, 1,642/3,375 (48.7%) were LP (p = 0.63). LP was most common in central Europe (4,791/9,625, 49.8%), followed by northern (5,704/11,692; 48.8%), southern (3,550/7,760; 45.8%) and eastern Europe (541/1,377; 38.3%; p < 0.0001). There was a significant increase in LP in male and female people who inject drugs (PWID) (adjusted odds ratio (aOR)/year later 1.16; 95% confidence interval (CI): 1.02-1.32), and a significant decline in LP in northern Europe (aOR/year later 0.89; 95% CI: 0.85-0.94). Further improvements in effective HIV testing strategies, with a focus on vulnerable groups, are required across the European continent.

Anion exchange membrane soil nitrate predicts turfgrass color and yield.

Desirable nitrogen (N) management practices for turfgrass supply sufficient N for high quality turf while limiting excess soil N. Previous studies suggested the potential of anion exchange membranes (AEMs) for predicting turfgrass color, quality, or yield. However, these studies suggested a wide range of critical soil nitrate-nitrogen (NO3-N) values across sample dates. A field experiment, in randomized complete block design with treatments consisting of nine N application rates, was conducted on a mixed species cool-season turfgrass lawn across two growing seasons. Every 2 wk from May to October, turfgrass color was assessed with three different reflectance meters, and soil NO3-N was measured with in situ AEMs. Cate-Nelson models were developed comparing relative reflectance value and yield to AEM desorbed soil NO3-N pooled across all sample dates. These models predicted critical AEM soil NO3-N values from 0. 45 to 1.4 micro g cm-2 d-1. Turf had a low probability of further positive response to AEM soil NO3-N greater than these critical values. These results suggest that soil NO3-N critical values from AEMs may be applicable across sample dates and years and may serve to guide N fertilization to limit excess soil NO3-N.

Nitrogen Fertilizer Form and Associated Nitrate Leaching from Cool-Season Lawn Turf

Various N fertilizer sources are available for lawn turf. Few field studies, however, have determined the losses of nitrate (NO3-N) from lawns receiving different formulations of N fertilizers. The objectives of this study were to determine the differences in NO3-N leaching losses among various N fertilizer sources and to ascertain when losses were most likely to occur. The field experiment was set out in a completely random design on a turf typical of the lawns in southern New England. Treatments consisted of four fertilizer sources with fast- and slow-release N formulations: (i) ammonium nitrate (AN), (ii) polymer-coated sulfur-coated urea (PCSCU), (iii) organic product, and (iv) a nonfertilized control. The experiment was conducted across three years and fertilized to supply a total of 147 kg N ha-1 yr-1. Percolate was collected with zero-tension lysimeters. Flow-weighted NO3-N concentrations were 4.6, 0.57, 0.31, and 0.18 mg L-1 for AN, PCSCU, organic, and the control, respectively. After correcting for control losses, average annual NO3-N leaching losses as a percentage of N applied were 16.8% for AN, 1.7% for PCSCU, and 0.6% for organic. Results indicate that NO3-N leaching losses from lawn turf in southern New England occur primarily during the late fall through the early spring. To reduce the threat of NO3-N leaching losses, lawn turf fertilizers should be formulated with a larger percentage of slow-release N than soluble N.

Factors associated with needle sharing among Black male injection drug users in Harris County, Texas

Background. Injection drug users (IDUs) are at increased risk for HIV transmission due to unique risk behaviors, such as sharing needles. In Houston, IDUs account for 18% of all HIV/AIDS cases among Black males. ^ Objectives. This analysis compared demographic, behavioral, and psychosocial characteristics of needle sharing and non-sharing IDUs in a population of Black males in Harris County, Texas. ^ Methods. Data used for this analysis were from the second IDU cycle of the National HIV Behavioral Surveillance System. This dataset included a sample of 288 Black male IDUs. Univariate and multivariate statistical analysis were performed to determine statistically significant associations of needle sharing in this population and to create a functional model to inform local HIV prevention programs. ^ Results. Half of the participants in this analysis shared needles in the past 12 months. Compared to non-sharers, sharers were more likely to be homeless (OR=3.70, p<0.01) or arrested in the past year (OR=2.31, p<0.01), inject cocaine (OR=2.07, p<0.01), report male-to-male sex in the past year (OR=6.97, p<0.01), and to exchange sex for money or drugs. Sharers were less likely than non-sharers to graduate high school (OR=0.36, p<0.01), earn $5,000 or more a year (OR=1.15, p=0.05), get needles from a medical source (OR=0.59, p=0.03), and ever test for HIV (OR=0.17, p<0.01). Sharers were more likely to report depressive symptoms (OR=3.49, p<0.01), lower scores on the family support scale (mean difference 0.41, p=0.01) and decision-making confidence scale (mean difference 0.38, p<0.01), and greater risk-taking (mean difference -0.49, p<0.01) than non-sharers. In a multivariable logistic regression, sharers were less likely to have graduated high school (OR=0.33, p<0.01) and have been tested for HIV (OR=0.12, p<0.01) and were more likely to have been arrested in the past year (OR=2.3, p<0.01), get needles from a street source (OR=3.87, p<0.01), report male-to-male sex (OR=7.01, p<0.01), and have depressive symptoms (OR=2.36, p=0.02) and increased risk-taking (OR=1.78, p=0.01). ^ Conclusions. IDUs that shared needles are different from those that did not, reporting lower socioeconomic status, increased sexual and risk behaviors, increased depressive symptoms and increased risk-taking. These findings suggest that intervention programs that also address these demographic, behavioral, and psychosocial factors may be more successful in decreasing needle sharing among this population.^

Phosphorus and Potassium Rates and Placement Methods for Corn and Soybean Managed with No-till or Tillage

No-till management for corn and soybean results in little or no incorporation of crop residues and fertilizer with soil. Subsurface banding phosphorus (P) and potassium (K) fertilizers with planter attachments could be more effective than broadcast fertilization, because in no-till with broadcast fertilizer, both nutrients accumulate at or near the soil surface. A long-term study was initiated in 1994 at the ISU Northwest Research Farm to evaluate P and K fertilizer placement for corn and soybean managed with no-till and chiselplow tillage.

Desarrollo de malezas en cultivos regados con efluentes domésticos tratados : Mendoza (Argentina)

La incidencia en el crecimiento de malezas por la utilización de efluentes domésticos para riego es uno de los aspectos económicos negativos del aprovechamiento de este tipo de aguas. El objetivo de este trabajo es comparar el crecimiento de malezas en cultivos regados con: a. efluentes domésticos tratados b. agua de perforación y agregado de fertilizante nitrogenado c. agua de perforación sin fertilizante La información se obtuvo de un ensayo de riego con cinco bloques al azar, realizado en Mendoza, Argentina, utilizando los efluentes de una planta depuradora con zanja de oxidación y se determinó el número y el peso de malezas (peso seco al aire). Se concluyó que el crecimiento de malezas (peso seco) es mayor cuando se utilizan efluentes que cuando se usa agua de perforación sin agregado de fertilizante: 1 200 % y también -pero en menor grado: 180 %- cuando se utiliza agua de perforación fertilizada En cuanto al número de malezas, no se encontraron diferencias significativas entre las medias de los tratamientos. Se interpretó que el aporte de nitrógeno y fósforo fue responsable del mayor crecimiento de malezas en parcelas regadas con efluentes.

Prótidos y nitratos en A. Tricolor L. fertilizado con NPK

Se evaluó la incidencia de la fertilización de nitrógeno (N), fósforo (P) y potasio (K) sobre el contenido de prótido crudo (Pr.C.) y acumulación de nitratos (NO3 -) en hojas de Amaranthus tricolor L. El ensayo, realizado en el invernáculo de la Facultad de Agronomía (UNLPam, Santa Rosa, Argentina), se efectuó en macetas utilizando un suelo con baja fertilidad. Las dosis de fertilizantes agregados -en kg/ha- fueron: 50 y 100 para N; 60 y 90, para P y 120, para K. Se practicaron todas las combinaciones posibles, originando 18 tratamientos, incluido el testigo. Para el análisis estadístico se empleó ANOVA triple y los tests de Tukey y Tukey- Kramer. Las variables analizadas fueron % Pr.C., base seca (b.s.) y contenido de NO3 -,expresado en % NO3 - (b.s.) y NO3 - mg/kg fresco. ¬ Para % Pr.C. se encontraron diferencias altamente significativas (p < 0.01) para las distintas dosis de N y diferencias significativas (p < 0.05) para la interacción PxK. ¬ Para NO3 - expresado como % (b.s.) y mg/kg fresco se encontraron diferencias altamente significativas para el factor N, significativas para P y no significativas para K. ¬ Las diferencias fueron altamente significativas para % (b.s.) en las interacciones dobles NxP y PxK y significativas, para NxK , así como diferencias significativas para todas las interacciones dobles en mg/kg fresco. ¬No se encontró significancia en la interacción triple.

Metales pesados en fertilizantes fosfatados, nitrogenados y mixtos

Para cuantificar los niveles de metales contaminantes: plomo y cadmio, en fertilizantes de uso común en Mendoza (Argentina) se analizaron 44 muestras de los principales productos comercializados por empresas agroquímicas; 61 % de ellos correspondieron a fertilizantes fosfatados; 32 %, a nitrogenados y el resto abarcó potásicos, magnésicos simples y orgánicos. Las muestras, molidas a polvo impalpable, se extractaron con ClH p.a. 1:5 para obtener extractos finales límpidos de dilución 1:50. Mediante espectrofotometría de absorción atómica y de llama aire-acetileno se cuantificó los tenores metálicos contrastándolos con patrones de alta confiabilidad. Valores detectados (mg/kg) • Pb: máximo: 21,5 en un fertilizante completo. mínimo: 0 en productos de fertirriego y una urea. • Cd: máximo: 30,30 en un superfosfato triple. mínimo: 0,25 en un producto de fertirriego. Valores medios para el total de las muestras: 9,55 mg Pb/kg y 8,20 mg Cd/kg. Finalmente se plantearon intervalos de confianza del 95 y 99 % para ambas determinaciones.

Reseña sobre las metodologías de campo, analíticas y estadísticas empleadas en la determinación y manejo de datos de los elementos de tierras raras en el sistema suelo-planta

El presente artículo es una revisión detallada de estudios científicos publicados que tratan el tema relacionado con la determinación de los elementos de las tierras raras (REEs) en el sistema suelo-planta. Los estudios han sido llevados a cabo principalmente en países europeos y asiáticos. Cabe señalar que la investigación en los países latinoamericanos es muy escasa; sin embargo, es creciente el interés de analizar la aportación de estos elementos al suelo y la planta, lo cual se debe a la aplicación de fertilizantes que contienen dosis elevadas de estos elementos en su composición. Diversas técnicas de muestreo, experimentación y análisis han sido empleadas para la determinación de los REEs. No obstante, se considera que el manejo de los datos ha sido incorrecto estadísticamente. El contenido del presente artículo aborda: (i) las generalidades de los REEs; (ii) el análisis de la bibliografía disponible con el fin de conocer las metodologías de muestreo y análisis más utilizadas en 37 artículos en total, señalando algunos puntos que se consideran todavía deficientes; (iii) dos ejemplos de la aplicación de técnicas estadísticas (intervalo de confianza de la media y pruebas de significancia de la relación F de Fisher y t de Student) utilizando datos reportados en dos artículos. Los resultados mostraron, con los datos del primer artículo analizado, que: a) no se aplicó una metodología estadística para evaluar la calidad de datos; b) al aplicar estadística se encontró que existen diferencias sistemáticas entre los datos determinados en el laboratorio y los certificados. En el segundo artículo analizado se demostró, mediante pruebas de significancia, que existen diferencias significativas en las medias de Ce y Eu (los dos elementos tomados como ejemplos) en las plantas de un sitio a otro.