Models for Internal Quality Fruit Sorting, Based on Time- Domain Laser Reflectance Spectroscopy (TDRS)

Time domain laser reflectance spectroscopy (TDRS) was applied for the first time to evaluate internal fruit quality. This technique, known in medicine-related knowledge areas, has not been used before in agricultural or food research. It allows the simultaneous non-destructive measuring of two optical characteristics of the tissues: light scattering and absorption. Models to measure firmness, sugar & acid contents in kiwifruit, tomato, apple, peach, nectarine and other fruits were built using sequential statistical techniques: principal component analysis, multiple stepwise linear regression, clustering and discriminant analysis. Consistent correlations were established between the two parameters measured with TDRS, i.e. absorption & transport scattering coefficients, with chemical constituents (sugars and acids) and firmness, respectively. Classification models were built to sort fruits into three quality grades, according to their firmness, soluble solids and acidity.

Decelerator elements for ramp transfer points in fruit packing lines

Two different decelerator elements used to reduce impacts on fruits on ramp transfer points in fruit packing lines were designed and tested. The performance of these elements, a powered decelerator and a multiple curtain, was compared to commercial decelerators (blankets). A ramp of length 60 cm was placed at an angle of 30º in an experimental fruit packing line between a roller transporter and a conveyor. The decelerators were placed on top of the ramp. Different tests were carried out to study the performance of the decelerators using instrumented spheres (IS 100) of various sizes. Results showed that decelerators can reduce the impact intensity down to safe thresholds. The powered decelerator was the most effective because it reduced the speed of fruits and did not cause retention of the fruit, when correctly regulated.

Fruit quality sensing:Post-harvest ripeness

Firmness sensing of selected varieties of apples, pears and avocado fruits has been developed using a nondestructive impact technique. In addition to firmness measurements, postharvest ripeness of apples and pears was monitored by spectrophotometric reflectance measurements, and that of avocadoes by Hunter colour measurements. The data obtained from firmness sensing were analyzed by three analytical procedures: principal component, correlation and regression, and stepwise discriminant analysis. A new software was developed to control the impact test, analyse the data, and sort the fruit into specified classes, based on the criteria obtained from a training procedure. Similar procedures were used to analyse the reflectance and colour data. Both sensing systems were able to classify fruits w i th good accuracy.

Insect growth regulators as potential insecticides to control olive fruit fly (Bactrocera oleae Rossi): insect toxicity bioassays and molecular docking approach

Olive fruit fly, Bactrocera oleae (Rossi), is a key pest in olive orchards, causing serious economic damage. To date, the pest has already developed resistance to the insecticides commonly applied to control it. Thus, in searching for new products for an accurate resistance management programme, targeting the ecdysone receptor (EcR)might provide alternative compounds for use in such programmes. RESULTS: Residual contact and oral exposure in the laboratory of B. oleae adults to the dibenzoylhydrazine-based compounds methoxyfenozide, tebufenozide and RH-5849 showed different results. Methoxyfenozide and tebufenozide did not provoke anynegative effectsontheadults,but RH-5849 killed98-100%of the treated insects15 days after treatment. Theligand-binding domain (LBD) of the EcR of B. oleae (BoEcR-LBD) was sequenced, and a homology protein model was constructed. Owing to a restricted extent of the ligand-binding cavity of the BoEcR-LBD, docking experiments with the three tested insecticides showed a severe steric clash in the case of methoxyfenozide and tebufenozide, while this was not the case with RH-5849. CONCLUSION: IGR molecules similar to the RH-5849 molecule, and different from methoxyfenozide and tebufenozide, might have potential in controlling this pest.

Relationship between solar radiation on watercore on apple fruit assessed with MRI

This work is a preliminary studio of the possibility of assess a relationship between solar radiation and watercore development on apple fruit, during maturation, using a non destructive method such as Magnetic Resonance Imaging (MRI). For such purpose, several low cost solar radiation sensors were designed for the trial and placed at 2 different heights (1.5 and 2.5 m) on 6 adult ?Esperiega? apple trees, in a commercial orchard in Ademuz (Valencia). Sensors were connected along 27 days, during the end of the growth period and start of the fruit maturation process, and radiation measurements of the a-Si sensors were recorded every 1 minute. At the end of this period, fruits from the upper and the lower part of the canopy of each tree were harvested. In all, 152 apples were collected and images with MRI. A Principal Component Analysis, perfomed over the histograms of the images, as well as segmentation methods were performed on the MR images in order to find a pattern involving solar radiation and watercore incidence.

Canopy fruit location can affect olive oil quality in Arbequina hedgerow orchards.

En este trabajo se recogieron muestras de aceituna procedente de distintas alturas de setos cultivados en diferentes condiciones para evaluar el efecto en la calidad del aceite.

Influence of climate change on the flowering of temperate fruit trees

It is well known that winter chilling is necessary for the flowering of temperate trees. The chilling requirement is a criterion for choosing a species or variety at a given location. Also chemistry products can be used for reducing the chilling-hours needs but make our production more expensive. This study first analysed the observed values of chilling hours for some representative agricultural locations in Spain for the last three decades and their projected changes under climate change scenarios. Usually the chilling is measured and calculated as chilling-hours, and different methods have been used to calculate them (e.g. Richarson et al., 1974 among others) according to the species considered. For our objective North Carolina method (Shaltout and Unrath, 1983) was applied for apples, Utah method (Richardson et al. 1974) for peach and grapevine and the approach used by De Melo-Abreu et al. (2004) for olive trees. The influence of climate change in temperate trees was studied by calculating projections of chilling-hours with climate data from Regional Climate Models (RCMs) at high resolution (25 km) from the European Project ENSEMBLES (http://www.ensembles-eu.org/). These projections will allow for analysing the modelled variations of chill-hours between 2nd half of 20C and 1st half of 21C at the study locations.

Canopy fruit location can affect olive oil quality in Arbequina hedgerow orchards.

The effect of location of fruit in canopies of hedgerow olive trees (Olea europaea L., cv. ‘Arbequina’) on quality of virgin oil was tested by analyzing oils extracted from different height layers and faces of nine olive hedgerows (6 North–South oriented and 3 East– West). Although sensory attributes were not different, other oil quality parameters may be significantly modified by fruit position. Oils extracted from fruits harvested from higher layers exhibited significantly higher stability against oxidation, along with higher palmitic acid, linoleic acid and phenol contents, but lower oleic acid content. Oils extracted from fruits harvested from East and North facing hedgerows oriented North–South and East–West, respectively, exhibited higher oleic contents and lower saturated and polyunsaturated fatty acid contents. The mean phenol content of oils extracted from fruits from a North–South oriented hedgerow was significantly greater from one of the East–West oriented hedgerows. These findings may be relevant for the design of future olive hedgerows destined for olive oil production.

Relations between resistance of fruit and skin characteristics in tomato varieties.

Varietal differences in physical properties of processing tomato fruits related with resistance are determined. Mean values of skin resistance and firmness are highly correlated with rupture of the fruits by quasi-static compression and by impact. Skin resistance is dependent on the features of the epidermis, studied microscopically.

Fruit firmness and skin resistance of processing tomato varieties tested in Badajoz (Spain), related to mechanical harvesting.

Determinations of resistance to damage were carried out in a total of 31 tomato varieties for processing, with the purpose of choosing the most suitable ones for mechanical harvesting. The characteristics studied include: puncture, deformation and rupture of the fruits, the ease of detachment of the fruits also being determined. Seventeen varieties were chosen, for further tests, with values 0.76 to 1.7 2 N of resistance to puncture; 3 to 9 N/mm of resistance to compression and 2.16 to 29.40 N resistance to detachment.

Impact parameters in relation to bruising and other fruit properties.

Impact response in fruits, primarily appl.es and pears (Pomaceae fruits), has been studied during the last five years. Using a laboratory impact testing device and also free-fall tests of instrumented apples, a significant body of results has been established, relative to the parameters which best characterize the impact response of these materials, and to their correlation with bruise damage, variety and ripeness level of the fruits. Bruise damage, measured as the size and/or volume of the affected fruit tissue is related primarily to applied energy (i.e. mainly drop height) for a given variety at a given ripeness stage. The relevant impact response parameters are maximum deformation (DM), permanent deformation (DP), maximum impulse (IM), maximum impact force (FM), maximum value of the force/time slope during impact (F/T) and impact time (T). The effect of ripeness differences was also studied in selected varieties of pears, being the most relevant parameters: maximum force (FM) and F/T slope.

Use of electronic fruits to evaluate fruit damage along the handling process.

Two electronic fruits (SEP-1, Simulated Electronic Product, developed in Scotland, and Techmark IS-100, Instrumented Sphere, developed in USA) have been compared in laboratory tests and then used to evaluate handling operations, in several cooperatives of two areas of Spain: Lérida (pome fruits) and Valencia (stone fruits). Advantages of each device were evaluated. Harvest, mechanical bin unloading, and grading line transfers and sizers were identified as operations causing fruit damage.

Neural bruise prediction models for fruit handling and machinery evaluation.

Some neural bruise prediction models have been implemented in the laboratory, for the most traded fruit species and varieties, allowing the prediction of the acceptability or rejectability for damages, with respect to the EC Standards. Different models have been built for both quasi-static (compression) and dynamic (impact) loads covering the whole commercial ripening period of fruits. A simulation process has been developed gathering the information on laboratory bruise models and load sensor calibrations for different electronic devices (IS-100 and DEA-1, for impact and compression loads respectively). Some evaluation methodology has been designed gathering the information on the mechanical properties of fruits and the loading records of electronic devices. The evaluation system allows to determine the current stage of fruit handling process and machinery.

Influence of fruit turgidity and firmness on apple bruise susceptibility.

Bruise damage is a major cause of quality loss for apples. It would be very useful to establish a method of characterizing bruise susceptibility in order to improve fruit handling, sometimes Magness-Taylor firmness is used as an indirect guide to handling requirements. The objective of the present work was to achieve a better bruise susceptibility prediction.

A quality assessment system for fresh fruits in hypermarkets and fruit trading centres.

When the fresh fruit reaches the final markets from the suppliers, its quality is not always as good as it should, either because it has been mishandled during transportation or because it lacks an adequate quality control at the producer level, before being shipped. This is why it is necessary for the final markets to establish their own quality assessment system if they want to ensure to their customers the quality they want to sell. In this work, a system to control fruit quality at the last level of the distribution channel has been designed. The system combines rapid control techniques with laboratory equipment and statistical sampling protocols, to obtain a dynamic, objective process, which can substitute advantageously the quality control inspections carried out visually by human experts at the reception platform of most hypermarkets. Portable measuring equipment have been chosen (firmness tester, temperature and humidity sensors...) as well as easy-to-use laboratory equipment (texturometer, colorimeter, refractometer..,) combining them to control the most important fruit quality parameters (firmness, colour, sugars, acids). A complete computer network has been designed to control all the processes and store the collected data in real time, and to perform the computations. The sampling methods have been also defined to guarantee the confidence of the results. Some of the advantages of a quality assessment system as the proposed one are: the minimisation of human subjectivity, the ability to use modern measuring techniques, and the possibility of using it also as a supplier's quality control system. It can be also a way to clarify the quality limits of fruits among members of the commercial channel, as well as the first step in the standardisation of quality control procedures.