Preparation and evaluation of atrazine immunoconjugate

Funda����o de Amparo �� Pesquisa do Estado de S��o Paulo (FAPESP)

Validated HPLC method for the standardization of Phyllanthus niruri (herb and commercial extracts) using corilagin as a phytochemical marker

Conselho Nacional de Desenvolvimento Cient��fico e Tecnol��gico (CNPq)

Development of an enzyme-linked immunosorbent assay for detection of IgM antibodies to Babesia bigemina in cattle

A crude antigenic preparation of Babesia bigemina was used to develop an ELISA for the detection of IgM antibodies. Optimal dilutions of the antigen, using positive and negative reference sera, were determined by checkerboard titrations. Negative sera from cattle imported from tick-free areas, serum samples collected from infected B. bigemina cattle were used to validate the test. The specificity was 94% and sensitivity of the Elisa 87.5%. Sera from 385 cattle deriving from areas free from tick-borne diseases, which were submitted to a preimmunization process, were screened by this technique. The Elisa detected seroconversion on the 14th day post-inoculation in animals either infested with Boophilus microplus ticks (infected with B. bigemina), or inoculated with B. bigemina infected blood. Antibody titers decreased after day 33; however, all animals remained positive until the end of the experiment (124 days). The ELISA described may prove to be an appropriate serological test for the detection of IgM antibodies against B. bigemina.

Determina����o de carbaril utilizando testes ELISA (Enzyme-linked immunosorbent assay) e CLAE com detec����o por arranjo de diodos

Funda����o de Amparo �� Pesquisa do Estado de S��o Paulo (FAPESP)

Callus cell culture of Pothomorphe umbellata (L.) under stress condition leads to high content of peroxidase enzyme

Funda����o de Amparo �� Pesquisa do Estado de S��o Paulo (FAPESP)

Maternal protein restriction affects gene expression and enzyme activity of intestinal disaccharidases in adult rat offspring

This study investigated the consequences of intrauterine protein restriction on the gastrointestinal tract and particularly on the gene expression and activity of intestinal disaccharidases in the adult offspring. Wistar rat dams were fed isocaloric diets containing 6% protein (restricted, n = 8) or 17% protein (control, n = 8) throughout gestation. Male offspring (n = 5-8 in each group) were evaluated at 3 or 16 weeks of age. Maternal protein restriction during pregnancy produced offspring with growth restriction from birth (5.7 �� 0.1 vs 6.3 �� 0.1���g; mean �� SE) to weaning (42.4 �� 1.3 vs 49.1 �� 1.6���g), although at 16 weeks of age their body weight was similar to control (421.7 �� 8.9 and 428.5 �� 8.5���g). Maternal protein restriction also increased lactase activity in the proximal (0.23 �� 0.02 vs 0.15 �� 0.02), medial (0.30 �� 0.06 vs 0.14 �� 0.01) and distal (0.43 �� 0.07 vs 0.07 �� 0.02 U��g-1��min-1) small intestine, and mRNA lactase abundance in the proximal intestine (7.96 �� 1.11 vs 2.38 �� 0.47 relative units) of 3-week-old offspring rats. In addition, maternal protein restriction increased sucrase activity (1.20 �� 0.02 vs 0.91 �� 0.02 U��g-1��min-1) and sucrase mRNA abundance (4.48 �� 0.51 vs 1.95 �� 0.17 relative units) in the duodenum of 16-week-old rats. In conclusion, the present study shows for the first time that intrauterine protein restriction affects gene expression of intestinal enzymes in offspring.

Xilanase e ��-glucanase na digestibilidade aparente de nutrientes do triticale pela Til��pia-do-nilo

Avaliou-se o efeito de diferentes n��veis do composto enzim��tico Natugrain Blend L��, que cont��m endo-xilanase e endo-beta-glucanase, sobre a digestibilidade dos nutrientes e a energia do triticale pela til��pia-do-nilo. O m��todo para a determina����o da digestibilidade foi o indireto, utilizando-se o ��xido de cr��mio III (0,10%). O delineamento experimental foi inteiramente ao acaso, com cinco tratamentos e tr��s repeti����es. O n��vel de substitui����o da dieta-refer��ncia foi 50,0% pelo triticale. Os tratamentos foram 0,0; 150,0; 300,0; 450,0 e 600,0mg kg-1 de Natugrain Blend L, que cont��m 800 unidades g-1 de endo-1,3(4)-��-glucanase (BGU) e 36.600 unidades g-1 de endo-1,4-��-xylanase (EXU). Os coeficientes de digestibilidade aparente foram: da mat��ria seca, 76,42; 74,01; 83,39; 82,97 e 78,34%; da prote��na bruta 88,19; 88,39; 90,52; 92,05 e 88,34%, da energia bruta 75,93; 71,31; 81,78; 80,27 e 78,62%, respectivamente, para os n��veis de inclus��o na dieta 0,0; 150,0; 300,0; 450,0 e 600,0mg kg-1 de Natugrain Blend L.Os resultados demonstram que 300mg kg-1 do complexo de enzimas foi suficiente para aumentar o coeficiente de digestibilidade aparente da mat��ria seca. O composto de enzimas pode ser utilizado para aumentar a efici��ncia de aproveitamento dos nutrientes do triticale.

Teste imunoenzim��tico (enzyme-linked immunosorbent assay) para diagn��stico da cisitcercose bovina e estudo da cin��tica de produ����o de anticorpos contra-Cysticercus bovis

Conselho Nacional de Desenvolvimento Cient��fico e Tecnol��gico (CNPq)

C��dmio e a atividade de peroxidase durante a germina����o de sementes de feijoeiro

Funda����o de Amparo �� Pesquisa do Estado de S��o Paulo (FAPESP)

Enzyme activity and microbial biomass in an Oxisol amended with sewage sludge contaminated with nickel

Funda����o de Amparo �� Pesquisa do Estado de S��o Paulo (FAPESP)

Preparation of silica with controlled pore sizes for enzyme immobilization

Funda����o de Amparo �� Pesquisa do Estado de S��o Paulo (FAPESP)

A deterministic simulation study of embryo marker-assisted selection for age at first calving in Nellore (Bos indicus) beef cattle

Funda����o de Amparo �� Pesquisa do Estado de S��o Paulo (FAPESP)

Exploitation of mitochondrial nad6 as a complementary marker for studying population variability in Lepidoptera

Conselho Nacional de Desenvolvimento Cient��fico e Tecnol��gico (CNPq)

Lipoperoxidation and cyclooxygenase enzyme inhibitory piperidine alkaloids from Cassia spectabilis green fruits

Phytochernical work in the search for bioactive metabolites from the methanolic extract of Senna spectabilis green fruits led to the isolation of a new piperidine alkaloid, (+)-3-O-feruloylcassine (1), in addition to the known (-)-spectaline (2) and (-)-3-O-acetylspectaline (3). The isolates were submitted to in vitro evaluation of lipoperoxidation (LPO) and cyclooxygenase enzymes (COX-1 and -2) inhibitory properties and showed moderate antioxidant activities (40-70%) at 100 ppm when compared to commercial standards BHT and vitamin E and moderate inhibition of COX-1 (ca. 40%) and marginal inhibition of COX-2 enzymes (< 10%) at 100 ppm when compared to nonsteroidal anti-inflammatory drugs (NSAIDs) aspirin, rofecoxib, and celecoxib, respectively.

Atrazine interaction with tropical humic substances by Enzyme Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay (ELISA) has been evaluated by analyzing rich-humic water samples from tropical rivers. The samples were spiked with atrazine at ppb level Different pHs (4 to 9) and humic concentrations (2.5 to 40 mg L-1) were investigated. The assay performance showed a strong dependence on the pH values and amount of humic matter at low atrazine concentration. From all the conditions studied the low pH (pH 4) and high humic substances concentrations (40 mg L-1) showed the greatest influence. The IC50 value to control sample (no humic) diminished from 0.28 nmol L-1 to 0.64 nmol L-1 to humic acid solution. This effect is specially noted for the humic acid fractions, since fulvic acid fractions showed no significant change on the immunoassay results. Additionally, it has been demonstrated that at basic pH the matrix effect produced by the natural Brazilian water sample containing humic substances even at 40 mg L-1 disappears. Therefore, the ELISA method used to determine atrazine, can be employed to determine this pesticide in water samples containing humic substances without prior preparation.