Ecualización y optimización de la codificación para la simulación de sistemas OFDM

Los sistemas basados en la técnica OFDM (Multiplexación por División de Frecuencias Ortogonales) son una evolución de los tradicionales sistemas FDM (Multiplexación por División de Frecuencia), gracias a la cual se consigue un mejor aprovechamiento del ancho de banda. En la actualidad los sistemas OFDM y sus variantes ocupan un lugar muy importante en las comunicaciones, estando implementados en diversos estándares como pueden ser: DVB-T (estándar de la TDT), ADSL, LTE, WIMAX, DAB (radio digital), etc. Debido a ello, en este proyecto se implementa un sistema OFDM en el que poder realizar diversas simulaciones para entender mejor su funcionamiento. Para ello nos vamos a valer de la herramienta Matlab. Los objetivos fundamentales dentro de la simulación del sistema es poner a prueba el empleo de turbo códigos (comparándolo con los códigos convolucionales tradicionales) y de un ecualizador. Todo ello con la intención de mejorar la calidad de nuestro sistema (recibir menos bits erróneos) en condiciones cada vez más adversas: relaciones señal a ruido bajas y multitrayectos. Para ello se han implementado las funciones necesarias en Matlab, así como una interfaz gráfica para que sea más sencillo de utilizar el programa y más didáctico. En los capítulos segundo y tercero de este proyecto se efectúa un estudio de las bases de los sistemas OFDM. En el segundo nos centramos más en un estudio teórico puro para después pasar en el tercero a centrarnos únicamente en la teoría de los bloques implementados en el sistema OFDM que se desarrolla en este proyecto. En el capítulo cuarto se explican las distintas opciones que se pueden llevar a cabo mediante la interfaz implementada, a la vez que se elabora un manual para el correcto uso de la misma. El quinto capítulo se divide en dos partes, en la primera se muestran las representaciones que puede realizar el programa, y en la segunda únicamente se realizan simulaciones para comprobar que tal responde nuestra sistema a distintas configuraciones de canal, y las a distintas configuraciones que hagamos nosotros de nuestro sistema (utilicemos una codificación u otra, utilicemos el ecualizador o el prefijo cíclico, etc…). Para finalizar, en el último capítulo se exponen las conclusiones obtenidas en este proyecto, así como posibles líneas de trabajo que seguir en próximas versiones del mismo. ABSTRACT. Systems based on OFDM (Orthogonal Frequency Division Multiplexing) technique are an evolution of traditional FDM (Frequency Division Multiplexing). Due to the use of OFDM systems are achieved by more efficient use of bandwidth. Nowadays, OFDM systems and variants of OFDM systems occupy a very important place in the world of communications, being implemented in standards such as DVB-T, ADSL, LTE, WiMAX, DAB (digital radio) and another more. For all these reasons, this project implements a OFDM system for performing various simulations for better understanding of OFDM system operation. The system has been simulated using Matlab. With system simulation we search to get two key objectives: to test the use of turbo codes (compared to traditional convolutional codes) and an equalizer. We do so with the intention of improving the quality of our system (receive fewer rates of bit error) in increasingly adverse conditions: lower signal-to-noise and multipath. For these reasons necessaries Matlab´s functions have been developed, and a GUI (User Graphical Interface) has been integrated so the program can be used in a easier and more didactic way. This project is divided into five chapters. In the second and third chapter of this project are developed the basis of OFDM systems. Being developed in the second one a pure theoretical study, while focusing only on block theory implemented in the OFDM system in the third one. The fourth chapter describes the options that can be carried out by the interface implemented. Furthermore the chapter is developed for the correct use of the interface. The fifth chapter is divided into two parts, the first part shows to us the representations that the program can perform, and the second one just makes simulations to check that our system responds to differents channel configurations (use of convolutional codes or turbo codes, the use of equalizer or cyclic prefix…). Finally, the last chapter presents the conclusions of this project and possible lines of work to follow in future versions.

Estructura y pruebas con Labmu

La implantación de la televisión digital en España ha supuesto un conjunto de desafíos técnicos y de orden práctico que se han ido acometiendo en multitud de ámbitos, desde la legislación que normaliza las infraestructuras comunes de telecomunicación hasta los cambios en las instalaciones y receptores donde el usuario final recibe los servicios. Por la complejidad y el carácter interdisciplinar de los conocimientos necesarios el aprendizaje de los titulados dentro del ámbito de la Telecomunicación supone también un reto importante. Este proyecto realiza una primera aproximación a un conjunto de herramientas hardware y software de ayuda a la enseñanza de esta amplia disciplina. El proyecto se ha realizado en torno a Labmu laboratorio multiusuario para prácticas de televisión digital de la empresa Xpertia. Se ha realizado para conocer y documentar sus posibilidades. También se ha documentado la tarjeta moduladora DTA-111 y el software para Windows StreamXpress. Estos sistemas ofrecen muchas posibilidades para la docencia de la televisión digital en todas las áreas desde la codificación fuente hasta la decodificación en el usuario final. En particular para ambos sistemas se han realizado pruebas en radiofrecuencia de emisiones de TDT. También se han establecido algunas ideas para trabajo futuro con estos sistemas. El proyecto se divide en seis capítulos: Capitulo 1: En el primer capítulo titulado Introducción se presenta el proyecto. Capítulo 2: En el segundo capítulo titulado Composición Hardware Labmu se presentan todos los componentes del laboratorio Labmu con la descripción de cada componente y sus características técnicas. Así mismo se presenta el interconexionado y configuración con que se ha trabajado. Capítulo 3: En el tercer capítulo titulado Software Labmu se describe como manual de usuario todos los componentes y posibilidades de software de Labmu. Capítulo 4: En el cuarto capítulo titulado Medidas con Labmu se realiza las medidas de MER, CBER, VBER, C/N y potencia de canal de los canales emitidos en la Comunidad de Madrid, comparando estas medidas con el analizador Promax Prodig-5. Capítulo 5: En el quinto capítulo titulado Tarjeta receptora y software se describe la tarjeta DTA-111 y el software StreamXpress, realizando medidas con el analizador Promax Prodig-5 introduciendo errores a la señal emitida por la tarjeta, y estudiando los niveles límites de visualización correcta. Capítulo 6: En el sexto y último capítulo titulado Conclusiones se presentan las conclusiones del proyecto y un plan de trabajo futuro ABSTRACT. The implantation of Digital Television in Spain (TDT) has implied a number of technical and practical challenges in several scopes. These challenges range from recommendations that standardize common telecommunications infrastructure to the changes in facilities where the end user receives digital services. The complexity and the interdisciplinary nature of skills that graduate Telecommunications students need to learn, is also a major challenge. This project is a first approach of a set of hard ware and software tools to help in the task of teaching this broad range discipline. The project has been carried out on Labmu, a multiuser Digital Television laboratory created by the Xpertia company. Its objectives are to understand and document this range of possibilities. DTA-111 modulator card and software for Windows StreamXpress have also been documented. These systems offer many options for teaching digital television in all areas, from source coding to end user decoding. In particular, both systems were tested on RF emissions in TDT. More over some ideas for future work with these systems have also foreseen. The project is structured in six chapters: Chapter1: This section Introduces the project. Chapter2: Titled “The Composition Hardware Labmu” presents Labmu lab components and provides descriptions for each component and its technical characteristics. It also presents the interconnection and configuration we have been using. Chapter3: Titled “Software Labmu” is a user manual, describing all components and software possibilities Labmu offers. Chapter4: Titled “Measures to Labmu” presents MER, CBER, VBER, C /N and channel power measures provided by Labmu in comparison with Promax Prodig-5 measures for all channels broad casting digital television services in the Community of Madrid. Chapter 5 Titled “Receiver card and software” describes DTA-111 card and software StreamXpress. Also the effects of errors insertion performed by this card are measured with the PromaxProdig-5 meter. Threes hold levels for correct reception are also studied. Chapter6: Entitled Conclusions presents the conclusions of the project and a plan for future work.

Planificación de una red 4G

La intensa evolución tecnológica que está experimentando nuestra sociedad en las últimas décadas hace que se estén desarrollando continuamente nuevas tecnologías que proporcionan mejoras tanto en la calidad como en la seguridad del servicio, este es el caso del 4G. A día de hoy, en España, la cuarta generación de comunicaciones móviles se ve encabezada por LTE, mientras que LTE-Advanced sólo se está implantando en las principales ciudades de nuestro país durante los últimos meses. Por este motivo, se ha creído interesante realizar una planificación sobre una zona que, hasta el momento, no está cubierta por cobertura LTE-Advanced. Además hay que tener en cuenta la naturaleza del terreno en el que trabajaremos, ya que se aleja del suelo urbano que encontramos en las principales ciudades con LTE-Advanced, como Madrid, Barcelona o Valencia. El estudio de esta zona semirural es de gran interés ya que uno de los objetivos de la cuarta generación es hacer llegar conexión a internet de calidad a lugares en los que no puede llegar la fibra óptica, como por ejemplo estas zonas semirurales. Para añadir aún más interés en el estudio, se ha decidido utilizar la banda de 800 MHz para el despliegue de la red. Esta banda que anteriormente era utilizada para la transmisión TDT, recientemente ha quedado liberada, en el conocido como Dividendo Digital para su uso en comunicaciones móviles. La tecnología LTE-Advanced se está empezando a desplegar en esta banda aunque realmente hasta Noviembre del año 2015 no tendremos un uso real de la misma, por lo que en estos momentos las redes 4G están utilizando la banda de 2.6 GHz. La utilización de la banda de 800 MHz conllevará mejoras tanto al usuario como a las operadoras, las cuales iremos viendo a lo largo del desarrollo del proyecto. La planificación pasará por distintas fases de optimización y expansión en las que se analizaran tanto la parte radioeléctrica como su capacidad. Se analizaran señales del tipo RSRP, RSSI o RSRQ y para el análisis de capacidad se definirá un conjunto de usuarios, distribuidos adecuadamente por toda la zona, que permitirá estudiar en detalle la capacidad de nuestra red. Para finalizar, se realizarán varias pruebas que demostrarán lo importante que es la tecnología MIMO tanto en LTE como en LTE-Advanced. ABSTRACT. Nowadays, our society is experiencing an intense pace of technological evolution which causes the constant development of new technologies. In the network planning area, these new technologies are focused on improving both quality and safety of service, with the recent deployment of 4G technologies in our networks. This project focuses on Spain, where the fourth generation of mobile communications is led by LTE, because LTE-Advanced has only been deployed in the largest cities, so far. The goal of this project is to plan, deploy and simulate LTE-Advanced network, of an area that hasn´t yet been covered. Furthermore, it will be taken into account the nature of the terrain where the network will be developed, as it moves away from urban areas in the major cities with LTE-Advanced, including Madrid, Barcelona and Valencia. The study of these semi-rural areas is extremely important because one of the main objectives of the fourth generation technologies is to get high-speed internet access to places that can be reached through other technologies, such as optical fiber. In order to adjust to the actual needs, the project was developed for the 800 MHz band. Those frequencies used to be assigned for digital terrestrial TV, but they have recently been released through the Digital Dividend in 2015 to use with mobile communications. That is the reason why, the LTE-Advanced technology in Spain is starting to be deployed in those frequencies. Despite the freeing of the 800 MHz band, it is not allowed to use it until November 2015, so 4G networks are currently using the 2.6 GHz band. The use of the 800 MHz band will led to advantages and improvements to users and operators, which will be detailed over the project. Each step of the planning of the 4G network is detailed. It is analyzed the optimization and expansion of the network, based on the radio and capacity premises. RSRP, RSSI or RSRQ signals were analyzed and an analysis of the network capacity was carried out. Finally, several tests are developed to show the importance of MIMO in LTE and LTE-Advanced.

Association of terminal deoxynucleotidyl transferase with Ku

Terminal deoxynucleotidyl transferase (TdT) catalyzes the addition of nucleotides at the junctions of rearranging Ig and T cell receptor gene segments, thereby generating antigen receptor diversity. Ku is a heterodimeric protein composed of 70- and 86-kDa subunits that binds DNA ends and is required for V(D)J recombination and DNA double-strand break (DSB) repair. We provide evidence for a direct interaction between TdT and Ku proteins. Studies with a baculovirus expression system show that TdT can interact specifically with each of the Ku subunits and with the heterodimer. The interaction between Ku and TdT is also observed in pre-T cells with endogenously expressed proteins. The protein–protein interaction is DNA independent and occurs at physiological salt concentrations. Deletion mutagenesis experiments reveal that the N-terminal region of TdT (131 amino acids) is essential for interaction with the Ku heterodimer. This region, although not important for TdT polymerization activity, contains a BRCA1 C-terminal domain that has been shown to mediate interactions of proteins involved in DNA repair. The induction of DSBs in Cos-7 cells transfected with a human TdT expression construct resulted in the appearance of discrete nuclear foci in which TdT and Ku colocalize. The physical association of TdT with Ku suggests a possible mechanism by which TdT is recruited to the sites of DSBs such as V(D)J recombination intermediates.

Ku80 is required for addition of N nucleotides to V(D)J recombination junctions by terminal deoxynucleotidyl transferase

V(D)J recombination generates a remarkably diverse repertoire of antigen receptors through the rearrangement of germline DNA. Terminal deoxynucleotidyl transferase (TdT), a polymerase that adds random nucleotides (N regions) to recombination junctions, is a key enzyme contributing to this diversity. The current model is that TdT adds N regions during V(D)J recombination by random collision with the DNA ends, without a dependence on other cellular factors. We previously demonstrated, however, that V(D)J junctions from Ku80-deficient mice unexpectedly lack N regions, although the mechanism responsible for this effect remains undefined in the mouse system. One possibility is that junctions are formed in these mice during a stage in development when TdT is not expressed. Alternatively, Ku80 may be required for the expression, nuclear localization or enzymatic activity of TdT. Here we show that V(D)J junctions isolated from Ku80-deficient fibroblasts are devoid of N regions, as were junctions in Ku80-deficient mice. In these cells TdT protein is abundant at the time of recombination, localizes properly to the nucleus and is enzymatically active. Based on these data, we propose that TdT does not add to recombination junctions through random collision but is actively recruited to the V(D)J recombinase complex by Ku80.

Transcription of the lymphocyte-specific terminal deoxynucleotidyltransferase gene requires a specific core promoter structure.

The terminal deoxynucleotidyltransferase (TdT) gene encodes a template-independent DNA polymerase that is expressed exclusively in immature lymphocytes. The TdT promoter lacks a TATA box, but an initiator element (Inr) overlaps the transcription start site. The Inr directs basal transcription and also mediates activated transcription in conjunction with an upstream element called D'. We have begun to address the fundamental question of why the TdT promoter contains an Inr rather than a TATA box. First, we tested the possibility that the TdT promoter lacks a TATA box because the -30 region is needed for the binding of an essential regulator. Mutations were introduced into the -30 region, and the mutants were tested in transient transfection and in vitro transcription assays. The mutations had only minor effects on promoter strength, suggesting that this first hypothesis is incorrect. Next, the effect of inserting a TATA box within the -30 region was tested. Although the TATA box enhanced promoter strength, appropriate regulation appeared to be maintained, as transcription in lymphocytes remained dependent on the D' element. Finally, a promoter variant containing a TATA box at -30, but a mutant Inr, was tested. Surprisingly, transcription from this variant, both in vitro and in vivo, was dramatically reduced. These results suggest that the TdT promoter, and possibly other natural promoters, contain an Inr element because one or more activator proteins that interact with surrounding control elements preferentially function in its presence.

Morbilidade global das colostomias derivativas temporárias na abordagem cirúrgica do cancro do recto

Trabalho Final do Curso de Mestrado Integrado em Medicina, Faculdade de Medicina, Universidade de Lisboa, 2014

Lack of association between genetic markers on chromosome 16q22-Q24 and type 1 diabetes in Russian affected families

Aim To evaluate whether the T1D susceptibility locus on chromosome 16q contributes to the genetic susceptibility to T1D in Russian patients. Method Thirteen microsatellite markers, spanning a 47-centimorgan genomic region on 16q22-q24 were evaluated for linkage to T1D in 98 Russian multiplex families. Multipoint logarithm of odds (LOD) ratio (MLS) and nonparametric LOD (NPL) values were computed for each marker, using GENEHUNTER 2.1 software. Four microsatellites (D16S422, D16S504, D16S3037, and D16S3098) and 6 biallelic markers in 2 positional candidate genes, ICSBP1 and NQO1, were additionally tested for association with T1D in 114 simplex families, using transmission disequilibrium test (TDT). Results A peak of linkage (MLS = 1.35, NPL = 0.91) was shown for marker D16S750, but this was not significant (P = 0.18). The subsequent linkage analysis in the subset of 46 multiplex families carrying a common risk HLA-DR4 haplotype increased peak MLS and NPL values to 1.77 and 1.22, respectively, but showed no significant linkage (P = 0.11) to T1D in the 16q22-q24 genomic region. TDT analysis failed to find significant association between these markers and disease, even after the conditioning for the predisposing HLA-DR4 haplotype. Conclusion Our results did not support the evidence for the susceptibility locus to T1D on chromosome 16q22-24 in the Russian family data set. The lack of association could reflect genetic heterogeneity of type 1 diabetes in diverse ethnic groups.

Association between polymorphisms in the progesterone receptor gene and endometriosis

The progesterone receptor (PR) is a candidate gene for the development of endometriosis, a complex disease with strong hormonal features, common in women of reproductive age. We typed the 306 base pair Alu insertion (AluIns) polymorphism in intron G of PR in 101 individuals, estimated linkage disequilibrium (LD) between five single-nucleotide polymorphisms (SNPs) across the PR locus in 980 Australian triads (endometriosis case and two parents) and used transmission disequilibrium testing (TDT) for association with endometriosis. The five SNPs showed strong pairwise LD, and the AluIns was highly correlated with proximal SNPs rs1042839 ({Delta}2 = 0.877, D9 = 1.00, P < 0.0001) and rs500760 ({Delta}2 = 0.438, D9 = 0.942, P < 0.0001). TDT showed weak evidence of allelic association between endometriosis and rs500760 (P = 0.027) but not in the expected direction. We identified a common susceptibility haplotype GGGCA across the five SNPs (P = 0.0167) in the whole sample, but likelihood ratio testing of haplotype transmission and non-transmission of the AluIns and flanking SNPs showed no significant pattern. Further, analysis of our results pooled with those from two previous studies suggested that neither the T2 allele of the AluIns nor the T1/T2 genotype was associated with endometriosis.

New concepts for distinguishing the hidden patterns of linkage disequilibrium which underlie association between genotypes and complex phenotypes

A disappointing feature of conventional methods for detecting association between DNA variation and a phenotype of interest is that they tell us little about the hidden pattern of linkage disequilibrium (LD) with the functional variant that is actually responsible for the association. This limitation applies to case-control studies and also to the transmission/disequilibrium test (TDT) and other family-based association methods. Here we present a fresh perspective on genetic association based on two novel concepts called 'LD squares' and 'equi-risk alleles'. These describe and characterize the different patterns of gametic LD which underlie genetic association. These concepts lead to a general principle - the Equi-Risk Allele Segregation Principle - which captures the way in which underlying LD patterns affect the transmission patterns of genetic variants associated with a phenotype. This provides a basis for distinguishing the hidden LD patterns and might help to locate the functional variants responsible for the association.

Facilitating soil-and plant-water research through the use of TDR

The development of TDR for measurement of soil water content and electrical conductivity has resulted in a large shift in measurement methods for a breadth of soil and hydrological characterization efforts. TDR has also opened new possibilities for soil and plant research. Five examples show how TDR has enhanced our ability to conduct our soil- and plant-water research. (i) Oxygen is necessary for healthy root growth and plant development but quantitative evaluation of the factors controlling oxygen supply in soil depends on knowledge of the soil water content by TDR. With water content information we have modeled successfully some impact of tillage methods on oxygen supply to roots and their growth response. (ii) For field assessment of soil mechanical properties influencing crop growth, water content capability was added to two portable soil strength measuring devices; (a) A TDT (Time Domain Transmittivity)-equipped soil cone penetrometer was used to evaluate seasonal soil strengthwater content relationships. In conventional tillage systems the relationships are dynamic and achieve the more stable no-tillage relationships only relatively late in each growing season; (b) A small TDR transmission line was added to a modified sheargraph that allowed shear strength and water content to be measured simultaneously on the same sample. In addition, the conventional graphing procedure for data acquisition was converted to datalogging using strain gauges. Data acquisition rate was improved by more than a factor of three with improved data quality. (iii) How do drought tolerant plants maintain leaf water content? Non-destructive measurement of TDR water content using a flat serpentine triple wire transmission line replaces more lengthy procedures of measuring relative water content. Two challenges remain: drought-stressed leaves alter salt content, changing electrical conductivity, and drought induced changes in leaf morphology affect TDR measurements. (iv) Remote radar signals are reflected from within the first 2 cm of soil. Appropriate calibration of radar imaging for soil water content can be achieved by a parallel pair of blades separated by 8 cm, reaching 1.7 cm into soil and forming a 20 cm TDR transmission line. The correlation between apparent relative permittivity from TDR and synthetic aperture radar (SAR) backscatter coefficient was 0.57 from an airborne flyover. These five examples highlight the diversity in the application of TDR in soil and plant research.

Serviço de videochamada utilizando o padrão o HbbTV: myPhoneOnTV

Nos últimos anos tem-se verificado que a população portuguesa está cada vez mais envelhecida. Este fator agrava-se em ambientes rurais, onde a desertificação populacional é mais frequente, derivado, entre outros, da migração da população mais jovem para os grandes centros urbanos, em busca de melhores condições de vida. Tendo como consequência a exclusão social da população residente nestas zonas. O reduzido foco populacional das zonas rurais não é um fator atrativo para as entidades que realizam investimentos em serviços e infraestruturas tecnológicas, devido ao retorno financeiro obtido não ser, à partida, rentável. Levando a uma exclusão tecnológica de quem reside nestas zonas. Este fator, agrava-se na população sénior dado que raramente são estimulados a interagir com a tecnologia. Com o objetivo de contribuir para a melhoria desta problemática, combatendo simultaneamente os dois tipos de exclusões, tecnológica e social, propõem-se através desta dissertação, um serviço de videochamada, baseado no conceito do padrão Hybrid Broadcast Broadband TV (HbbTV). Um dos fatores considerados, durante o estudo deste serviço, foi a barreira tecnológica existente entre os idosos e a tecnologia. De modo a aproximar ambos, optou-se por aproveitar os conhecimentos que os idosos já possuem na utilização de equipamentos do seu quotidiano. Sendo a televisão, o equipamento selecionado para integrar no serviço de videochamada, permitindo ao idoso contactar facilmente com outras pessoas e serviços. Através da implementação de um protótipo e dos resultados obtidos, conclui-se que o serviço desenvolvido é uma solução válida para combater a problemática apresentada, contribuindo positivamente para a redução da exclusão social e da iliteracia tecnológica

Enzymatic Polymerization of High Molecular Weight DNA

The use of DNA as a polymeric building material transcends its function in biology and is exciting in bionanotechnology for applications ranging from biosensing, to diagnostics, and to targeted drug delivery. These applications are enabled by DNA’s unique structural and chemical properties, embodied as a directional polyanion that exhibits molecular recognition capabilities. Hence, the efficient and precise synthesis of high molecular weight DNA materials has become key to advance DNA bionanotechnology. Current synthesis methods largely rely on either solid phase chemical synthesis or template-dependent polymerase amplification. The inherent step-by-step fashion of solid phase synthesis limits the length of the resulting DNA to typically less than 150 nucleotides. In contrast, polymerase based enzymatic synthesis methods (e.g., polymerase chain reaction) are not limited by product length, but require a DNA template to guide the synthesis. Furthermore, advanced DNA bionanotechnology requires tailorable structural and self-assembly properties. Current synthesis methods, however, often involve multiple conjugating reactions and extensive purification steps.

The research described in this dissertation aims to develop a facile method to synthesize high molecular weight, single stranded DNA (or polynucleotide) with versatile functionalities. We exploit the ability of a template-independent DNA polymerase−terminal deoxynucleotidyl transferase (TdT) to catalyze the polymerization of 2’-deoxyribonucleoside 5’-triphosphates (dNTP, monomer) from the 3’-hydroxyl group of an oligodeoxyribonucleotide (initiator). We termed this enzymatic synthesis method: TdT catalyzed enzymatic polymerization, or TcEP.

Specifically, this dissertation is structured to address three specific research aims. With the objective to generate high molecular weight polynucleotides, Specific Aim 1 studies the reaction kinetics of TcEP by investigating the polymerization of 2’-deoxythymidine 5’-triphosphates (monomer) from the 3’-hydroxyl group of oligodeoxyribothymidine (initiator) using in situ 1H NMR and fluorescent gel electrophoresis. We found that TcEP kinetics follows the “living” chain-growth polycondensation mechanism, and like in “living” polymerizations, the molecular weight of the final product is determined by the starting molar ratio of monomer to initiator. The distribution of the molecular weight is crucially influenced by the molar ratio of initiator to TdT. We developed a reaction kinetics model that allows us to quantitatively describe the reaction and predict the molecular weight of the reaction products.

Specific Aim 2 further explores TcEP’s ability to transcend homo-polynucleotide synthesis by varying the choices of initiators and monomers. We investigated the effects of initiator length and sequence on TcEP, and found that the minimum length of an effective initiator should be 10 nucleotides and that the formation of secondary structures close to the 3’-hydroxyl group can impede the polymerization reaction. We also demonstrated TcEP’s capacity to incorporate a wide range of unnatural dNTPs into the growing chain, such as, hydrophobic fluorescent dNTP and fluoro modified dNTP. By harnessing the encoded nucleotide sequence of an initiator and the chemical diversity of monomers, TcEP enables us to introduce molecular recognition capabilities and chemical functionalities on the 5’-terminus and 3’-terminus, respectively.

Building on TcEP’s synthesis capacities, in Specific Aim 3 we invented a two-step strategy to synthesize diblock amphiphilic polynucleotides, in which the first, hydrophilic block serves as a macro-initiator for the growth of the second block, comprised of natural and/or unnatural nucleotides. By tuning the hydrophilic length, we synthesized the amphiphilic diblock polynucleotides that can self-assemble into micellar structures ranging from star-like to crew-cut morphologies. The observed self-assembly behaviors agree with predictions from dissipative particle dynamics simulations as well as scaling law for polyelectrolyte block copolymers.

In summary, we developed an enzymatic synthesis method (i.e., TcEP) that enables the facile synthesis of high molecular weight polynucleotides with low polydispersity. Although we can control the nucleotide sequence only to a limited extent, TcEP offers a method to integrate an oligodeoxyribonucleotide with specific sequence at the 5’-terminus and to incorporate functional groups along the growing chains simultaneously. Additionally, we used TcEP to synthesize amphiphilic polynucleotides that display self-assemble ability. We anticipate that our facile synthesis method will not only advance molecular biology, but also invigorate materials science and bionanotechnology.

Diversity and television in Argentina: the case of the Audiovisual Technology Hub Programme

This paper describes and analyses the Audiovisual Technology Hub Programme (Programa Polos Audiovisuales Tecnológicos - PPAT), which has been implemented in Argentina between 2010 and 2015 as part of the public policy of former administration of Cristina Fernández de Kirchner. The main goal was to promote a television industry that reflects the cultural diversity of Argentina by dividing the national territory in nine into nine audiovisual technology hubs, where national public universities acted as centres that gathered a range of regional stakeholders. Considering the 18 TV seasons that were produced for television between 2013 and 2014, the text analyses the diversity of sources and genres / subgenres and its restricted marketing. The article closes with a brief set of conclusions about this initiative.

Fútbol y redes sociales. Análisis de la gestión de Relaciones Públicas 2.0 por los clubes de fútbol

Las relaciones públicas se configuran como una actividad esencial en la gestión de la comunicación con los públicos de los clubes de fútbol ya que son muy activos y su grado de reactividad es alto. Con este texto se analiza el papel que desempeñan las redes sociales en los clubes de fútbol que poseen mayor número de ingresos. Para ello, se desarrolla una metodología que estudia qué presencia tienen en las redes sociales, el número de seguidores, qué grado de interacción se produce entre clubes y públicos y los contenidos y las temáticas de los textos de las redes sociales. Los resultados muestran un público activo pero con intervenciones relacionadas con los resultados futbolísticos y una gestión comunicativa unidireccional por parte de los clubes.